##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933175.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421222
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23841595472066	33.0	31.0	34.0	30.0	34.0
2	32.41433850587734	34.0	31.0	34.0	30.0	34.0
3	32.492943396598136	34.0	31.0	34.0	30.0	34.0
4	35.97102634211967	37.0	35.0	37.0	35.0	37.0
5	35.157239333475	37.0	35.0	37.0	35.0	37.0
6	34.79240892696567	37.0	35.0	37.0	32.0	37.0
7	35.623326264299315	37.0	35.0	37.0	33.0	37.0
8	35.86680968912668	37.0	35.0	37.0	35.0	37.0
9	37.77408033368467	39.0	38.0	39.0	35.0	39.0
10	37.312000517864206	39.0	37.0	39.0	34.0	39.0
11	37.25428961837067	39.0	37.0	39.0	34.0	39.0
12	37.12189439791954	39.0	37.0	39.0	34.0	39.0
13	37.0531282234584	39.0	37.0	39.0	33.0	39.0
14	38.23572179434318	40.0	37.0	41.0	33.0	41.0
15	38.30242425180584	40.0	38.0	41.0	34.0	41.0
16	38.322981912748325	40.0	37.0	41.0	34.0	41.0
17	38.24719572311715	40.0	37.0	41.0	34.0	41.0
18	38.207534783446924	40.0	37.0	41.0	34.0	41.0
19	38.20685367943924	40.0	37.0	41.0	34.0	41.0
20	38.076068341188076	40.0	37.0	41.0	34.0	41.0
21	38.00832100825909	40.0	37.0	41.0	33.0	41.0
22	38.046706285154606	40.0	37.0	41.0	34.0	41.0
23	37.936249931397064	40.0	36.0	41.0	33.0	41.0
24	37.8838928752862	40.0	36.0	41.0	33.0	41.0
25	37.78938195440262	40.0	36.0	41.0	33.0	41.0
26	37.76842745186889	40.0	36.0	41.0	33.0	41.0
27	37.70672843510725	40.0	36.0	41.0	33.0	41.0
28	37.6422768575212	40.0	36.0	41.0	33.0	41.0
29	37.57918326623145	40.0	36.0	41.0	33.0	41.0
30	37.49079946693761	40.0	36.0	41.0	33.0	41.0
31	37.40380179873377	40.0	36.0	41.0	33.0	41.0
32	37.345178304304326	40.0	35.0	41.0	32.0	41.0
33	37.24392881618776	39.0	35.0	41.0	32.0	41.0
34	37.06764741891133	39.0	35.0	41.0	31.0	41.0
35	36.939108035197876	39.0	35.0	41.0	31.0	41.0
36	36.82406126558694	39.0	35.0	41.0	31.0	41.0
37	36.76811996999765	39.0	35.0	41.0	31.0	41.0
38	36.679370992005474	39.0	35.0	41.0	31.0	41.0
39	36.60343282048829	39.0	35.0	41.0	31.0	41.0
40	36.45992322100277	39.0	35.0	41.0	30.0	41.0
41	36.307486796573656	39.0	35.0	41.0	30.0	41.0
42	36.305317536598785	39.0	35.0	40.0	30.0	41.0
43	36.210230351064084	39.0	35.0	40.0	30.0	41.0
44	36.1672898393073	38.0	35.0	40.0	30.0	41.0
45	36.1710190244733	38.0	35.0	40.0	30.0	41.0
46	35.928589622170215	38.0	35.0	40.0	29.0	41.0
47	35.78359538481673	38.0	35.0	40.0	29.0	41.0
48	35.72068473468607	38.0	35.0	40.0	29.0	41.0
49	35.78847780290482	38.0	35.0	40.0	29.0	41.0
50	35.57328411747074	38.0	34.0	40.0	28.0	41.0
51	34.51449034703938	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	37.0
11	29.0
12	20.0
13	22.0
14	28.0
15	58.0
16	59.0
17	164.0
18	295.0
19	556.0
20	1123.0
21	1885.0
22	3073.0
23	4133.0
24	5688.0
25	8023.0
26	10915.0
27	12835.0
28	14916.0
29	17895.0
30	22671.0
31	29559.0
32	38517.0
33	53872.0
34	89258.0
35	114777.0
36	123485.0
37	183422.0
38	295710.0
39	388008.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.18674492795636	4.218130594657274	52.6120479418416	15.983076535544763
2	20.559490354075578	7.234900669986814	51.549089445561634	20.656519530375974
3	21.42944592751871	7.199719677854691	49.76555386843153	21.605280526195063
4	18.089714344416283	4.74324208322134	52.86091828018423	24.30612529217814
5	19.422792498286686	8.171840852449511	48.30223568168801	24.10313096757579
6	20.869997790633693	9.553820585383564	52.8822379614163	16.69394366256644
7	81.85863995913377	2.2853572489027045	11.414965431157132	4.441037360806405
8	84.42333428556552	4.703698648064834	7.00946087240417	3.863506193965475
9	79.02481104289126	5.312892707824675	8.576140814031868	7.086155435252199
10	38.18066424527625	28.67553415300354	16.43754459190753	16.706257009812685
11	30.789841418159863	24.86479944723625	24.52537323514553	19.819985899458352
12	26.41276310104966	21.685352464287774	30.204500071065603	21.69738436359696
13	24.414975281834927	22.714818656057957	32.9491803532453	19.921025708861812
14	21.34156380917267	25.731729455356025	30.17016342274465	22.756543312726656
15	19.32787418151422	23.994703149824588	34.783376559045664	21.894046109615527
16	21.27957490103587	22.56325894195277	31.80333543950206	24.353830717509297
17	21.275564267932808	22.561781340283222	30.899746837580615	25.262907554203355
18	22.059959668510622	22.445191532357367	31.256341373831813	24.2385074253002
19	23.76884118033636	24.795070720830385	28.54107240107457	22.895015697758687
20	26.541314446300433	24.385141800506887	28.713529624506233	20.36001412868644
21	25.65524597846079	25.633433763338875	27.471007344383917	21.24031291381642
22	23.618266534010875	23.819290723053825	28.029259327536444	24.53318341539886
23	22.961718858841195	25.85957718076416	27.22467003747479	23.954033922919855
24	22.67590847875983	24.30612529217814	28.91033209449333	24.107634134568702
25	22.04321351625573	26.871382514484015	27.14903090439073	23.936373064869528
26	21.431627149030906	25.91614821611261	28.4772540813469	24.174970553509585
27	21.698087983439603	25.98489187473878	28.30993328276652	24.007086859055097
28	20.506718865877392	25.38505595888609	30.394125618657746	23.714099556578773
29	21.5558160512573	23.965362202386398	29.499050816832273	24.97977092952403
30	22.53004808538005	26.38074839820943	28.36643395613071	22.722769560279815
31	23.62403621672054	26.542088428127343	26.26598800187444	23.567887353277673
32	24.209799735720384	27.304953061520298	25.399831975581577	23.085415227177737
33	23.98541536790171	25.620838968155574	25.363525191701225	25.030220472241492
34	22.513442657093684	25.825873790301586	27.22481076144332	24.435872791161408
35	21.90066013613637	26.053213361459367	27.484094673457065	24.5620318289472
36	22.85969398165804	27.141009638184606	26.47207825378442	23.527218126372937
37	22.040750846806482	28.939250870025933	26.555668291090345	22.46432999207724
38	23.803107466673048	26.606962177618982	25.67649529770859	23.913435057999383
39	22.292576388488218	26.239320809838297	26.43380133434467	25.034301467328817
40	23.33498918536302	26.275346145781587	28.007306388446	22.382358280409395
41	20.134081797213945	27.10484357827278	27.56268900988023	25.19838561463304
42	20.674602560331884	26.741705377485008	27.407540834577564	25.17615122760554
43	21.27760476547647	24.79851845805933	27.78960640913242	26.134270367331773
44	22.200120741164998	24.488714641343858	28.18567401855586	25.125490598935286
45	22.339437470008207	24.520377534262767	26.80073908228271	26.339445913446312
46	22.746974082866718	26.724255605387476	27.133551267852596	23.395219043893213
47	20.30682046858267	26.3302284935077	29.55217411495178	23.81077692295785
48	21.02416089815666	25.29829963228827	28.417868566627874	25.2596709029272
49	21.684367396508076	24.018063328600316	29.928258920844176	24.369310354047432
50	21.722855401900617	24.198190008316786	28.19376564674625	25.885188943036347
51	20.63773288057742	24.705359190893468	26.77139813484452	27.885509793684587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	279.0
1	1058.5
2	1838.0
3	4509.0
4	7180.0
5	5012.5
6	2845.0
7	2851.5
8	2858.0
9	2931.0
10	3004.0
11	3168.0
12	3332.0
13	3289.0
14	3246.0
15	3283.0
16	3320.0
17	3364.5
18	3409.0
19	3410.0
20	3411.0
21	4241.0
22	5071.0
23	5570.0
24	6069.0
25	7157.5
26	10094.5
27	11943.0
28	14304.5
29	16666.0
30	19504.0
31	22342.0
32	26331.0
33	30320.0
34	34855.0
35	39390.0
36	40729.0
37	42068.0
38	47033.0
39	51998.0
40	59133.5
41	66269.0
42	73689.5
43	81110.0
44	87824.5
45	94539.0
46	114215.0
47	133891.0
48	133172.5
49	132454.0
50	127342.0
51	122230.0
52	110446.0
53	98662.0
54	89188.0
55	79714.0
56	75565.5
57	71417.0
58	67322.0
59	63227.0
60	60375.0
61	57523.0
62	51404.0
63	45285.0
64	38530.0
65	31775.0
66	27649.5
67	23524.0
68	20181.5
69	16839.0
70	14739.0
71	12639.0
72	10717.0
73	8795.0
74	7126.5
75	4354.0
76	3250.0
77	2526.0
78	1802.0
79	1402.5
80	1003.0
81	767.0
82	531.0
83	402.5
84	274.0
85	196.5
86	119.0
87	76.5
88	34.0
89	22.0
90	10.0
91	6.0
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1421222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.404988030687363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35054685988264	22.450869165512835
2	9.70310538468612	5.706393953943869
3	3.4336780762991492	3.0290178820443026
4	1.5995052142578774	1.8813372672109963
5	0.9710687189204833	1.427713202841586
6	0.6400018535180877	1.1291548105390257
7	0.47301947369982306	0.9736392373697734
8	0.3556475812191871	0.8366250295114486
9	0.3121218168810068	0.8260144460552158
>10	5.361138337645244	41.82159335408195
>50	0.7257396924516324	12.92362158774932
>100	0.06941748160787076	3.632389938647319
>500	0.001669836310279727	0.33325900846054857
>1k	0.0026240284875824285	1.6381668877263
>5k	7.15644132977026E-4	1.390204228305538
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	7553	0.5314440671478489	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	6498	0.4572121737490695	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	5768	0.40584792523617	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3783	0.266179386471642	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	3337	0.23479794148978833	RNA PCR Primer, Index 33 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	2921	0.20552735603586209	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	2352	0.16549138698950622	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCGAACT	1878	0.13213980644825368	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1872	0.13171763454266822	Illumina Paired End PCR Primer 2 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1636	0.11511220625630621	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTC	1471	0.10350247885270562	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	1463	0.10293958297859167	RNA PCR Primer, Index 33 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.407239685284917E-4	0.0	0.0	0.22220314630648835	0.0
2	1.407239685284917E-4	0.0	0.0	0.820561460489635	0.0
3	1.407239685284917E-4	0.0	0.0	1.1622392560768127	0.0
4	1.407239685284917E-4	0.0	0.0	1.6836919214591386	0.0
5	1.407239685284917E-4	0.0	0.0	2.7641001898366335	0.0
6	1.407239685284917E-4	0.0	0.0	3.309405567884539	0.0
7	1.407239685284917E-4	0.0	0.0	3.8903844719544165	0.0
8	1.407239685284917E-4	0.0	0.0	4.74239773941017	0.0
9	1.407239685284917E-4	0.0	0.0	5.182863760904349	0.0
10	1.407239685284917E-4	0.0	0.0	6.44930911567651	0.0
11	1.407239685284917E-4	0.0	0.0	7.7433363682802545	0.0
12	1.407239685284917E-4	0.0	0.0	9.516669457692043	0.0
13	1.407239685284917E-4	0.0	0.0	9.918577111809414	0.0
14	1.407239685284917E-4	0.0	0.0	10.077173024341025	0.0
15	1.407239685284917E-4	0.0	0.0	10.371567566502629	0.0
16	2.1108595279273752E-4	0.0	0.0	10.865156886116313	0.0
17	2.1108595279273752E-4	0.0	0.0	11.53641021599722	0.0
18	2.1108595279273752E-4	0.0	0.0	12.262194083682916	0.0
19	2.814479370569834E-4	0.0	0.0	12.905584067795179	0.0
20	2.814479370569834E-4	0.0	0.0	13.44519012511768	0.0
21	2.814479370569834E-4	0.0	0.0	14.174421730032325	0.0
22	2.814479370569834E-4	0.0	0.0	14.952203104089298	0.0
23	4.2217190558547504E-4	0.0	0.0	15.688893079335951	0.0
24	4.925338898497209E-4	0.0	0.0	16.270434879279943	0.0
25	5.628958741139668E-4	0.0	0.0	16.795616729828275	0.0
26	5.628958741139668E-4	0.0	0.0	17.23516804552702	0.0
27	5.628958741139668E-4	0.0	0.0	17.67704130670648	0.0
28	5.628958741139668E-4	0.0	0.0	18.14382271031549	0.0
29	5.628958741139668E-4	0.0	0.0	18.643392798591634	0.0
30	5.628958741139668E-4	0.0	0.0	19.237318307766134	0.0
31	5.628958741139668E-4	0.0	0.0	19.74188409692504	0.0
32	5.628958741139668E-4	0.0	0.0	20.237936085987975	0.0
33	5.628958741139668E-4	0.0	0.0	20.739124499902196	0.0
34	5.628958741139668E-4	0.0	0.0	21.232432371578824	0.0
35	5.628958741139668E-4	0.0	0.0	21.760569425466255	0.0
36	5.628958741139668E-4	0.0	0.0	22.263235441050025	0.0
37	5.628958741139668E-4	0.0	0.0	22.76231299543632	0.0
38	5.628958741139668E-4	0.0	0.0	23.2328939461956	0.0
39	5.628958741139668E-4	0.0	0.0	23.73584140971643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	20	7.033851E-4	45.0	18
ATCGCTA	20	7.033851E-4	45.0	24
TATACGT	30	2.165867E-6	44.999996	13
CGATGAA	495	0.0	43.181816	19
TTACGGG	345	0.0	41.739132	3
CGGTCTA	60	3.6379788E-12	41.249996	31
TATTGCG	55	6.184564E-11	40.909092	1
CGGGTAC	95	0.0	40.263157	6
TCCGATA	45	1.9288564E-8	40.000004	14
TACGGGT	90	0.0	40.000004	4
CGTTAGG	155	0.0	39.19355	2
TTTGGGC	3835	0.0	38.898308	4
CCGATGA	550	0.0	38.454544	18
TCGTTTG	430	0.0	38.197674	1
CGTTTGG	965	0.0	38.00518	2
TTTGGGA	7745	0.0	37.940605	4
TTGGGAC	3010	0.0	37.599667	5
CGACGGT	60	1.5643309E-10	37.499996	28
ATACGTC	30	1.140058E-4	37.499996	14
TGGGCGA	2710	0.0	37.44465	6
>>END_MODULE