##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933173.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1341451
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24221682342478	33.0	31.0	34.0	30.0	34.0
2	32.425695012341116	34.0	31.0	34.0	31.0	34.0
3	32.49721681969748	34.0	31.0	34.0	30.0	34.0
4	35.97121847909465	37.0	35.0	37.0	35.0	37.0
5	35.18654874460565	37.0	35.0	37.0	35.0	37.0
6	34.864769566685624	37.0	35.0	37.0	32.0	37.0
7	35.65519351806365	37.0	35.0	37.0	35.0	37.0
8	35.94450486823596	37.0	35.0	37.0	35.0	37.0
9	37.82056444849644	39.0	38.0	39.0	35.0	39.0
10	37.35191296588545	39.0	37.0	39.0	34.0	39.0
11	37.243437889270645	39.0	37.0	39.0	34.0	39.0
12	36.85555566323332	39.0	35.0	39.0	33.0	39.0
13	36.64811014341933	39.0	35.0	39.0	33.0	39.0
14	37.32232261931297	40.0	36.0	41.0	31.0	41.0
15	37.53547166463777	40.0	36.0	41.0	32.0	41.0
16	37.70052875580249	40.0	36.0	41.0	33.0	41.0
17	37.74398095793286	40.0	36.0	41.0	33.0	41.0
18	37.73911905839274	40.0	36.0	41.0	33.0	41.0
19	37.743795338033216	40.0	36.0	41.0	33.0	41.0
20	37.61812917505	39.0	35.0	41.0	33.0	41.0
21	37.51246523354189	39.0	35.0	41.0	33.0	41.0
22	37.63955448242239	39.0	35.0	41.0	33.0	41.0
23	37.59562294858329	39.0	35.0	41.0	33.0	41.0
24	37.51850645308699	39.0	35.0	41.0	33.0	41.0
25	37.38633017531017	39.0	35.0	41.0	33.0	41.0
26	37.35554783588815	39.0	35.0	41.0	33.0	41.0
27	37.30403048639123	39.0	35.0	41.0	33.0	41.0
28	37.232025620018916	39.0	35.0	41.0	33.0	41.0
29	37.14507276076428	39.0	35.0	41.0	32.0	41.0
30	37.03391998664133	39.0	35.0	41.0	32.0	41.0
31	36.901207722086006	39.0	35.0	41.0	31.0	41.0
32	36.88248843975665	39.0	35.0	41.0	32.0	41.0
33	36.78284857218042	39.0	35.0	41.0	31.0	41.0
34	36.591725676152166	39.0	35.0	41.0	31.0	41.0
35	36.45757839831645	39.0	35.0	41.0	31.0	41.0
36	36.35330176055629	38.0	35.0	40.0	30.0	41.0
37	36.29853419916195	38.0	35.0	40.0	30.0	41.0
38	36.19555466431498	38.0	35.0	40.0	30.0	41.0
39	36.13903228668062	38.0	35.0	40.0	30.0	41.0
40	36.02106599495621	38.0	35.0	40.0	30.0	41.0
41	35.86712597031125	38.0	35.0	40.0	30.0	41.0
42	35.81746929257945	38.0	35.0	40.0	30.0	41.0
43	35.699992023562544	38.0	35.0	40.0	29.0	41.0
44	35.64171632061104	37.0	34.0	40.0	29.0	41.0
45	35.66629642081597	37.0	34.0	40.0	30.0	41.0
46	35.513281513823465	37.0	34.0	40.0	29.0	41.0
47	35.41398977674175	37.0	34.0	40.0	29.0	41.0
48	35.3146302026686	37.0	34.0	40.0	29.0	41.0
49	35.35872424710258	37.0	34.0	40.0	29.0	41.0
50	35.177893191775176	36.0	34.0	40.0	29.0	41.0
51	34.07546753478137	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	20.0
10	33.0
11	26.0
12	17.0
13	32.0
14	29.0
15	54.0
16	84.0
17	204.0
18	409.0
19	712.0
20	1124.0
21	1849.0
22	2722.0
23	3940.0
24	5542.0
25	8064.0
26	10984.0
27	13654.0
28	16207.0
29	19780.0
30	24758.0
31	31836.0
32	44046.0
33	66347.0
34	117218.0
35	114954.0
36	118792.0
37	169993.0
38	259655.0
39	308254.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.446289875664487	4.38189691610055	55.12329559559015	16.048517612644815
2	20.901098884715132	4.422673657107118	52.54347717508877	22.132750283088985
3	21.368428664185274	4.610082664219566	52.040290700144844	21.981197971450317
4	19.53943901044466	4.9480003369485726	50.86879804033095	24.643762612275815
5	17.974044523430226	7.780381094799586	49.80703730512706	24.438537076643126
6	22.20133273597023	7.640681620126266	52.362777320975574	17.795208322927934
7	83.9527496718106	2.190165723533696	9.259003869690357	4.598080734965347
8	86.63805088668911	2.445411722083028	6.863090787512924	4.053446603714932
9	81.45493201018897	4.636844730072139	9.024556245438706	4.883667014300187
10	46.19587297635172	22.440700405754665	15.790587952895782	15.572838664997827
11	36.88930866651111	21.154630321942435	22.66284791617435	19.2932130953721
12	31.782674134202445	19.264587375908622	29.20345208285655	19.749286407032386
13	28.570853501171495	20.414610746124907	31.732355486708048	19.282180265995553
14	22.769523448862465	21.44901304632074	35.46622276922526	20.315240735591537
15	20.967966776274345	19.352030003332214	37.9747750756457	21.70522814474774
16	27.124807391399315	19.181170240284587	31.051078272706196	22.642944095609906
17	27.901950947146037	19.055187256187516	27.687630781892146	25.355231014774297
18	28.696985577557438	18.48051102872934	30.106280438122603	22.716222955590627
19	28.332380385120292	21.746377616476487	26.867697739239077	23.053544259164145
20	26.975864194815912	25.951152893396777	26.35981485719568	20.713168054591634
21	26.294437888525184	23.436040526265963	29.545618885818413	20.723902699390436
22	24.582560227693744	20.60440522985931	30.625941610986906	24.18709293146004
23	23.295222859426097	22.75088691275343	29.806754029778205	24.14713619804227
24	23.97158002789517	24.100321219336376	28.24404320396347	23.684055548804988
25	26.14355649218645	23.050935144108877	25.482332191037916	25.323176172666763
26	23.13211589539983	22.110759170480325	27.053615823462803	27.703509110657045
27	22.13468848284432	21.806759993469758	30.551917289561825	25.506634234124093
28	20.77459407760701	22.601869170025594	29.118842208921535	27.50469454344587
29	23.472046314028617	24.182694708938307	27.333387503531625	25.011871473501458
30	25.952792908574374	22.394929073070877	28.606859288934146	23.045418729420604
31	26.29801610345812	22.657778778352693	28.65456882137327	22.389636296815908
32	28.000202765512867	22.517408388379447	25.647079170241778	23.835309675865908
33	27.37341878309383	22.923684875556393	25.404729654679898	24.29816668666988
34	22.680142621683537	22.162419648574566	30.841081783829598	24.3163559459123
35	22.420722039045778	25.3321217099991	28.134982194653404	24.112174056301722
36	25.706566993501813	25.812273426312256	25.524450762644328	22.956708817541603
37	23.386318247927058	27.604437284701415	26.307334371512635	22.70191009585889
38	25.693372325936615	25.042733577298016	26.053579295852032	23.21031480091334
39	24.05410260978597	22.31747562900173	26.133940039554183	27.494481721658115
40	25.25735192712965	21.20658898461442	28.06326880370584	25.47279028455009
41	21.41636183505771	22.015041921024324	26.859050386484483	29.70954585743348
42	21.19637616282667	23.153510638853003	28.76467347670545	26.88543972161488
43	21.868186016485133	22.71532840185739	29.71998231765454	25.696503264002935
44	23.717303129223506	21.6597549966417	28.68878550166946	25.93415637246534
45	22.784656316183	21.270549576540628	26.653750304707362	29.29104380256901
46	22.10367728675889	21.578946976072924	29.15097159717351	27.166404139994675
47	19.922158915979786	23.67555728833927	31.955844827727585	24.44643896795336
48	20.62855818065662	25.57134028749466	28.828410430198343	24.97169110165038
49	23.912763119935054	22.66098426256345	29.199426591056998	24.2268260264445
50	21.753981323208972	22.650995079209004	27.601380892779538	27.993642704802486
51	20.362204806586302	23.107366575447035	26.69579433016935	29.834634287797318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	860.5
2	1492.0
3	3937.5
4	6383.0
5	4292.0
6	2201.0
7	2116.0
8	2031.0
9	2159.0
10	2287.0
11	2282.5
12	2278.0
13	2306.5
14	2335.0
15	2235.0
16	2135.0
17	2122.0
18	2109.0
19	2160.0
20	2211.0
21	2666.0
22	3121.0
23	3584.0
24	4047.0
25	4194.0
26	5517.0
27	6693.0
28	8196.5
29	9700.0
30	10644.0
31	11588.0
32	15155.0
33	18722.0
34	22465.0
35	26208.0
36	26905.0
37	27602.0
38	31357.5
39	35113.0
40	41022.5
41	46932.0
42	54961.0
43	62990.0
44	75533.0
45	88076.0
46	102956.0
47	117836.0
48	123508.0
49	129180.0
50	117302.5
51	105425.0
52	101366.5
53	97308.0
54	94515.0
55	91722.0
56	90605.5
57	89489.0
58	85064.0
59	80639.0
60	72966.0
61	65293.0
62	59882.5
63	54472.0
64	48558.5
65	42645.0
66	37222.5
67	31800.0
68	27312.0
69	22824.0
70	19306.5
71	15789.0
72	12938.0
73	10087.0
74	8943.5
75	5935.5
76	4071.0
77	3129.5
78	2188.0
79	1636.0
80	1084.0
81	788.0
82	492.0
83	361.5
84	231.0
85	194.5
86	158.0
87	108.5
88	59.0
89	36.5
90	14.0
91	14.0
92	14.0
93	10.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1341451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.49590555007866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01104259400122	22.125186275655874
2	10.75445946468723	6.34425041225128
3	3.795455211667192	3.3585116532866786
4	1.8063792399839251	2.131231658007549
5	1.06408163325498	1.5693025676031165
6	0.6826632358768248	1.2081462196760338
7	0.5237544743531507	1.0814028754866138
8	0.40015927881324526	0.9442448234290458
9	0.3237299810515665	0.8593838050343184
>10	4.5517868726003865	35.5667387185392
>50	0.9964261953335987	18.12382986114838
>100	0.08536078940882044	4.6674058297496135
>500	0.0024742257725606323	0.4742209839712282
>1k	0.002226803195304569	1.5461443161610406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3710	0.27656619585806713	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	3078	0.22945303257442873	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2911	0.21700382645359392	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2909	0.21685473416472165	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2847	0.21223287320968118	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2015	0.15021048103881543	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1422	0.10600461738818638	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.454614443613669E-5	0.0	0.0	0.24272224628406106	0.0
2	7.454614443613669E-5	0.0	0.0	0.9412196196506618	0.0
3	7.454614443613669E-5	0.0	0.0	1.3914037859004913	0.0
4	7.454614443613669E-5	0.0	0.0	1.8741646172689126	0.0
5	7.454614443613669E-5	0.0	0.0	2.6911158141445344	0.0
6	7.454614443613669E-5	0.0	0.0	3.3140233970528925	0.0
7	7.454614443613669E-5	0.0	0.0	3.8940669469104723	0.0
8	7.454614443613669E-5	0.0	0.0	4.818140953340823	0.0
9	7.454614443613669E-5	0.0	0.0	5.36851513771282	0.0
10	7.454614443613669E-5	0.0	0.0	6.262696140224279	0.0
11	7.454614443613669E-5	0.0	0.0	7.787164793943274	0.0
12	7.454614443613669E-5	0.0	0.0	9.133468162459904	0.0
13	7.454614443613669E-5	0.0	0.0	9.562332131400998	0.0
14	7.454614443613669E-5	0.0	0.0	9.74713202345818	0.0
15	7.454614443613669E-5	0.0	0.0	9.9825487475875	0.0
16	7.454614443613669E-5	0.0	0.0	10.489387983608793	0.0
17	7.454614443613669E-5	0.0	0.0	11.16947245929967	0.0
18	7.454614443613669E-5	0.0	0.0	11.947063291913011	0.0
19	7.454614443613669E-5	0.0	0.0	12.464338988155363	0.0
20	7.454614443613669E-5	0.0	0.0	13.066970019777091	0.0
21	7.454614443613669E-5	0.0	0.0	13.857308243089014	0.0
22	7.454614443613669E-5	0.0	0.0	14.67783765489757	0.0
23	7.454614443613669E-5	0.0	0.0	15.441711996934663	0.0
24	7.454614443613669E-5	0.0	0.0	16.049263074089176	0.0
25	7.454614443613669E-5	0.0	0.0	16.591735367150942	0.0
26	7.454614443613669E-5	0.0	0.0	17.06107789252086	0.0
27	7.454614443613669E-5	0.0	0.0	17.650588802721828	0.0
28	7.454614443613669E-5	0.0	0.0	18.172411813774787	0.0
29	7.454614443613669E-5	0.0	0.0	18.79919579619382	0.0
30	7.454614443613669E-5	0.0	0.0	19.525647973723974	0.0
31	7.454614443613669E-5	0.0	0.0	20.048887361521217	0.0
32	7.454614443613669E-5	0.0	0.0	20.565343050174775	0.0
33	7.454614443613669E-5	0.0	0.0	21.107517158658794	0.0
34	7.454614443613669E-5	0.0	0.0	21.727517441934143	0.0
35	7.454614443613669E-5	0.0	0.0	22.473798893884307	0.0
36	7.454614443613669E-5	0.0	0.0	23.048773305920232	0.0
37	7.454614443613669E-5	0.0	0.0	23.643204261653985	0.0
38	7.454614443613669E-5	0.0	0.0	24.370923723639553	0.0
39	7.454614443613669E-5	0.0	0.0	25.902399714935544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	20	7.033708E-4	45.000004	15
ATTACGC	20	7.033708E-4	45.000004	38
TATGCGT	20	7.033708E-4	45.000004	11
ACGGGTA	20	7.033708E-4	45.000004	5
TTACGCC	20	7.033708E-4	45.000004	39
CGTCGAT	40	6.8157533E-9	45.000004	40
TCGAGTA	20	7.033708E-4	45.000004	32
CACGTTA	20	7.033708E-4	45.000004	44
TATACGT	20	7.033708E-4	45.000004	26
CGATAAC	20	7.033708E-4	45.000004	16
ATACCCG	20	7.033708E-4	45.000004	19
GTTATCG	25	3.8911556E-5	45.0	45
TAATGCG	50	1.0822987E-9	40.5	24
AAACACG	385	0.0	40.324673	40
TTATCGG	45	1.9286745E-8	40.0	2
CGTTTTT	1780	0.0	39.4382	1
AACACGT	400	0.0	39.375	41
TATGGGA	510	0.0	39.264706	4
ATGGGAC	270	0.0	39.166664	5
TTACGGG	370	0.0	38.918922	3
>>END_MODULE