##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1262657
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.174470184697824	33.0	31.0	34.0	30.0	34.0
2	32.35350217834297	34.0	31.0	34.0	30.0	34.0
3	32.43537714517878	34.0	31.0	34.0	30.0	34.0
4	35.91836579530308	37.0	35.0	37.0	35.0	37.0
5	35.11981638718987	37.0	35.0	37.0	35.0	37.0
6	34.830950923330725	37.0	35.0	37.0	32.0	37.0
7	35.65317580308825	37.0	35.0	37.0	35.0	37.0
8	35.92212928768462	37.0	35.0	37.0	35.0	37.0
9	37.78932520866712	39.0	38.0	39.0	35.0	39.0
10	37.26760394944945	39.0	37.0	39.0	34.0	39.0
11	37.17632579552483	39.0	37.0	39.0	34.0	39.0
12	37.01342882508868	39.0	37.0	39.0	33.0	39.0
13	36.945339866646286	39.0	37.0	39.0	33.0	39.0
14	38.057030531648735	40.0	37.0	41.0	33.0	41.0
15	38.149700195698436	40.0	37.0	41.0	33.0	41.0
16	38.166026086260956	40.0	37.0	41.0	34.0	41.0
17	38.11915825121153	40.0	37.0	41.0	33.0	41.0
18	38.08102279558106	40.0	37.0	41.0	33.0	41.0
19	38.10014279412382	40.0	37.0	41.0	34.0	41.0
20	38.01731269853967	40.0	37.0	41.0	34.0	41.0
21	37.904103806496934	40.0	36.0	41.0	33.0	41.0
22	37.94656189289728	40.0	36.0	41.0	33.0	41.0
23	37.868971541756785	40.0	36.0	41.0	33.0	41.0
24	37.81500993539813	40.0	36.0	41.0	33.0	41.0
25	37.72106597436992	39.0	36.0	41.0	33.0	41.0
26	37.70405660444602	39.0	36.0	41.0	33.0	41.0
27	37.64030532440718	39.0	36.0	41.0	33.0	41.0
28	37.571890069908136	39.0	36.0	41.0	33.0	41.0
29	37.52558929305425	39.0	36.0	41.0	33.0	41.0
30	37.40547591309437	39.0	36.0	41.0	33.0	41.0
31	37.34794643359202	39.0	35.0	41.0	32.0	41.0
32	37.3450105610629	39.0	35.0	41.0	33.0	41.0
33	37.22031874056058	39.0	35.0	41.0	32.0	41.0
34	37.11945286803938	39.0	35.0	41.0	32.0	41.0
35	37.04138574450543	39.0	35.0	41.0	31.0	41.0
36	36.90484510045088	39.0	35.0	41.0	31.0	41.0
37	36.869183000609034	39.0	35.0	41.0	31.0	41.0
38	36.79331837545747	39.0	35.0	41.0	31.0	41.0
39	36.795076572655915	39.0	35.0	41.0	31.0	41.0
40	36.73342879340945	39.0	35.0	41.0	31.0	41.0
41	36.66740928058847	39.0	35.0	41.0	31.0	41.0
42	36.59966641772073	39.0	35.0	40.0	31.0	41.0
43	36.44708658012429	39.0	35.0	40.0	30.0	41.0
44	36.360376570992756	38.0	35.0	40.0	30.0	41.0
45	36.37861351103269	38.0	35.0	40.0	30.0	41.0
46	36.17761118023343	38.0	35.0	40.0	30.0	41.0
47	36.101356900567616	38.0	35.0	40.0	30.0	41.0
48	36.02928903098783	38.0	35.0	40.0	30.0	41.0
49	36.102049091716914	38.0	35.0	40.0	30.0	41.0
50	35.91445182658473	38.0	35.0	40.0	30.0	41.0
51	34.92842395044735	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	18.0
10	18.0
11	15.0
12	16.0
13	19.0
14	26.0
15	32.0
16	48.0
17	92.0
18	189.0
19	344.0
20	695.0
21	1121.0
22	1802.0
23	2779.0
24	3932.0
25	5755.0
26	8067.0
27	10286.0
28	12426.0
29	15145.0
30	19599.0
31	25745.0
32	35103.0
33	49911.0
34	84807.0
35	107510.0
36	112814.0
37	166870.0
38	273623.0
39	323747.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.98691251860165	4.151879726639935	54.22953343623803	16.63167431852039
2	19.95965650212211	4.794572080937262	53.16740809261739	22.078363324323234
3	21.51526503238805	5.221528887100773	50.98589719931857	22.277308881192596
4	18.849774721084188	4.631186458396857	51.61076998741543	24.908268833103527
5	18.04266716931043	7.8337188959471975	49.77266193431787	24.350952000424503
6	22.195655668958395	7.666769360166696	52.31983032605054	17.817744644824366
7	83.21373104493144	1.8995657569712123	10.204909171691124	4.681794026406221
8	84.94943599093023	2.719503396409318	7.537914097019222	4.793146515641223
9	78.95485472301662	5.338583637519928	9.828084745104965	5.878476894358483
10	39.908225274163925	27.342421576089155	17.183922474591277	15.565430675155644
11	29.084779160136126	24.735537838066872	25.687102673172525	20.49258032862448
12	24.581339191878712	21.877437815653817	32.170732035699324	21.370490956768148
13	23.062953755453776	23.25120757260285	33.590357476337594	20.095481195605775
14	20.85356514081021	26.219392915098876	31.520040676129778	21.40700126796113
15	19.07572682050628	23.0490940928534	36.162552458822944	21.71262662781737
16	21.115156372633265	22.716303794300433	31.789789309369056	24.378750523697253
17	20.584133299858948	21.958536641384	30.938330837274098	26.518999221482954
18	21.863340558837436	21.356393699951767	32.43810472677853	24.342161014432264
19	23.32414899691682	22.873115976864657	30.290253014080626	23.512482012137898
20	25.883276297521814	24.034793296991978	29.203021881635316	20.878908523850896
21	24.932978631568194	25.53710152480048	28.407714842589872	21.122205001041454
22	23.23251682761035	23.014326139244467	28.949350457012475	24.80380657613271
23	22.622374880905898	24.75961405195552	27.60646794814427	25.01154311899431
24	22.41321277274826	23.49482084208142	29.082957604480075	25.009008780690245
25	21.034928725695103	24.932820235424188	28.617352139179523	25.41489889970119
26	20.573599956282663	25.108085568764913	29.24768959424452	25.070624880707904
27	20.733421665582974	25.702229504924933	29.544999156540534	24.019349672951563
28	19.573249108823696	24.947551076816584	31.0238647550364	24.455335059323318
29	21.362175159207926	23.522144176922158	30.946329842546312	24.169350821323608
30	21.63097341558317	25.044647913091207	30.487139421077934	22.83723925024769
31	22.999199307492056	26.26334784506006	26.46744127660956	24.27001157083832
32	23.380062835750326	25.699774364692868	26.90398104948533	24.01618175007148
33	22.56630264592839	24.488677447636213	28.134006305750493	24.811013600684905
34	20.632681717996256	24.18503203958003	29.205318625723375	25.976967616700342
35	20.127714810910643	24.237619559389444	29.356507745175453	26.27815788452446
36	21.550983362861015	25.606399837802346	29.898460151886063	22.944156647450576
37	21.04783801143145	27.583975695695663	28.444541946070867	22.92364434680202
38	21.020989865022727	27.405859231762864	27.563542593119113	24.0096083100953
39	22.24380809673569	23.252316345610883	29.85498041035689	24.648895147296535
40	21.036908677495155	22.245867246607748	30.638090946314	26.07913312958309
41	18.45742747238561	23.74342359009612	28.53047185419318	29.268677083325084
42	20.64099751555648	23.16876237964863	29.10719221451273	27.08304789028216
43	21.513918665164013	23.42290899270348	29.9554827637276	25.1076895784049
44	21.028355285718924	23.64735632875753	29.5213981310839	25.802890254439646
45	21.901118039182453	23.07158634530201	28.095832835045464	26.93146278047007
46	22.0549206950106	23.70105262157498	29.938455178247143	24.30557150516728
47	19.082933845058474	24.195723779300316	31.196595750073058	25.524746625568145
48	19.59360301332824	23.857864804139208	30.696697519595585	25.851834662936966
49	21.7025684726731	21.98783992802479	32.118936496609926	24.19065510269218
50	20.932604816668345	21.610777907222626	30.062558557074485	27.394058719034543
51	19.778292917237223	21.539895632780713	28.953072766396577	29.728738683585487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	761.5
2	1258.0
3	3408.0
4	5558.0
5	3774.0
6	1990.0
7	1961.0
8	1932.0
9	2095.5
10	2259.0
11	2210.5
12	2162.0
13	2173.0
14	2184.0
15	2163.5
16	2143.0
17	2081.0
18	2019.0
19	2228.5
20	2438.0
21	2867.0
22	3296.0
23	3824.0
24	4352.0
25	5346.0
26	7768.0
27	9196.0
28	10601.5
29	12007.0
30	13747.0
31	15487.0
32	17769.0
33	20051.0
34	24895.5
35	29740.0
36	31026.0
37	32312.0
38	37743.5
39	43175.0
40	52757.5
41	62340.0
42	72952.5
43	83565.0
44	101996.0
45	120427.0
46	132809.0
47	145191.0
48	141141.0
49	137091.0
50	118418.0
51	99745.0
52	88775.5
53	77806.0
54	72599.0
55	67392.0
56	62299.0
57	57206.0
58	54076.0
59	50946.0
60	47957.0
61	44968.0
62	39624.5
63	34281.0
64	30225.0
65	26169.0
66	22165.0
67	18161.0
68	15321.5
69	12482.0
70	10628.0
71	8774.0
72	7654.0
73	6534.0
74	5232.0
75	3362.0
76	2794.0
77	2063.0
78	1332.0
79	1047.0
80	762.0
81	517.5
82	273.0
83	245.0
84	217.0
85	147.0
86	77.0
87	44.5
88	12.0
89	8.0
90	4.0
91	5.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	3.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1262657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.341007832459344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95248814682915	23.177455102396294
2	11.261996881577163	7.059246649492852
3	4.406412127806033	4.143041910318382
4	2.128123239495494	2.6679010846986815
5	1.2481588856665489	1.9559278705914518
6	0.7879312987661181	1.4816736603641263
7	0.5245548134771691	1.1508053562419547
8	0.4075336357512692	1.0218011896056927
9	0.3143175907900533	0.8865927067347731
>10	4.084936131048788	33.725097530466094
>50	0.8034530220648715	15.518671139040654
>100	0.07467246370356495	4.219046468889973
>500	0.0027108815119245336	0.5357225075727219
>1k	0.0024644377381132124	2.0683553965239216
>5k	2.464437738113212E-4	0.38866142706256296
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	5032	0.39852469831474424	No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	4714	0.3733397114180652	No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	4641	0.36755825216190935	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	4350	0.344511613209288	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3066	0.24282128875854647	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC	2154	0.17059264709259916	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1941	0.1537234577561444	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	1842	0.14588284862793302	No Hit
TCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	1728	0.1368542684196896	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.919807200213518E-5	0.0	0.0	0.4221257237713805	0.0
2	1.5839614400427036E-4	0.0	0.0	1.6149278861955385	0.0
3	1.5839614400427036E-4	0.0	0.0	2.2763901835573717	0.0
4	1.5839614400427036E-4	0.0	0.0	3.059579917586486	0.0
5	1.5839614400427036E-4	0.0	0.0	4.405392755118769	0.0
6	1.5839614400427036E-4	0.0	0.0	5.1876321122838585	0.0
7	1.5839614400427036E-4	0.0	0.0	5.9699506675209495	0.0
8	1.5839614400427036E-4	0.0	0.0	7.2250817126107885	0.0
9	1.5839614400427036E-4	0.0	0.0	7.893275846092803	0.0
10	1.5839614400427036E-4	0.0	0.0	9.20661747410421	0.0
11	1.5839614400427036E-4	0.0	0.0	11.193063516061764	0.0
12	1.5839614400427036E-4	0.0	0.0	13.225840430140568	0.0
13	1.5839614400427036E-4	0.0	0.0	13.81119338030835	0.0
14	1.5839614400427036E-4	0.0	0.0	14.005624647073592	0.0
15	1.5839614400427036E-4	0.0	0.0	14.346176356682774	0.0
16	1.5839614400427036E-4	0.0	0.0	15.115664824255518	0.0
17	1.5839614400427036E-4	0.0	0.0	16.216676421229202	0.0
18	1.5839614400427036E-4	0.0	0.0	17.441870595102234	0.0
19	1.5839614400427036E-4	0.0	0.0	18.27741025472476	0.0
20	1.5839614400427036E-4	0.0	0.0	19.125542407795624	0.0
21	1.5839614400427036E-4	0.0	0.0	20.202636187024662	0.0
22	1.5839614400427036E-4	0.0	0.0	21.316557069734696	0.0
23	1.5839614400427036E-4	0.0	0.0	22.40917367107615	0.0
24	1.5839614400427036E-4	0.0	0.0	23.275283786491503	0.0
25	1.5839614400427036E-4	0.0	0.0	23.994323082198886	0.0
26	1.5839614400427036E-4	0.0	0.0	24.648578355008524	0.0
27	1.5839614400427036E-4	0.0	0.0	25.275351896833424	0.0
28	1.5839614400427036E-4	0.0	0.0	25.92992396193107	0.0
29	1.5839614400427036E-4	0.0	0.0	26.624728647605803	0.0
30	1.5839614400427036E-4	0.0	0.0	27.44252793909985	0.0
31	2.3759421600640554E-4	0.0	0.0	28.14976672207892	0.0
32	3.167922880085407E-4	0.0	0.0	28.810199444504722	0.0
33	3.167922880085407E-4	0.0	0.0	29.498985076707292	0.0
34	3.167922880085407E-4	0.0	0.0	30.17327746173347	0.0
35	3.167922880085407E-4	0.0	0.0	30.899048593561037	0.0
36	3.167922880085407E-4	0.0	0.0	31.595912429107827	0.0
37	3.167922880085407E-4	0.0	0.0	32.25642434960563	0.0
38	3.167922880085407E-4	0.0	0.0	32.91986659876752	0.0
39	3.167922880085407E-4	0.0	0.0	33.619660762978384	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	25	3.8910242E-5	45.000004	38
ATGCGTA	25	3.8910242E-5	45.000004	34
CGTTAGT	20	7.033552E-4	45.0	31
GTATCGT	20	7.033552E-4	45.0	24
CGCAAAT	20	7.033552E-4	45.0	44
TAAGCGA	20	7.033552E-4	45.0	16
TTATTCG	20	7.033552E-4	45.0	1
CGATAAG	20	7.033552E-4	45.0	18
TCGTTAG	65	0.0	44.999996	1
CCCGTCA	70	0.0	41.785713	5
TCGTTTG	440	0.0	40.397728	1
CTAGCGG	80	0.0	39.375	2
AATCGTT	75	0.0	39.0	22
TATGCGT	35	6.2487798E-6	38.571426	11
TATCGAT	35	6.2487798E-6	38.571426	8
TGGGCGA	1990	0.0	38.55527	6
ACGGGAC	170	0.0	38.38235	5
CACGGGA	165	0.0	38.181816	4
TTGGGAC	2960	0.0	38.158787	5
TATGCGG	95	0.0	37.894737	2
>>END_MODULE