##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1741064
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11840977700992	33.0	31.0	34.0	30.0	34.0
2	32.378863154944334	34.0	31.0	34.0	30.0	34.0
3	32.30144670155721	33.0	31.0	34.0	30.0	34.0
4	35.93783399116862	37.0	35.0	37.0	35.0	37.0
5	35.1716364246231	37.0	35.0	37.0	35.0	37.0
6	34.978863499561186	37.0	35.0	37.0	33.0	37.0
7	35.76470480120202	37.0	35.0	37.0	35.0	37.0
8	36.0248767420382	37.0	35.0	37.0	35.0	37.0
9	37.905225769988924	39.0	38.0	39.0	35.0	39.0
10	37.42876941915978	39.0	37.0	39.0	35.0	39.0
11	37.29967824272973	39.0	37.0	39.0	34.0	39.0
12	37.302010724476524	39.0	37.0	39.0	34.0	39.0
13	37.34369155872501	39.0	37.0	39.0	34.0	39.0
14	38.592333193954964	40.0	38.0	41.0	34.0	41.0
15	38.650657299214735	40.0	38.0	41.0	34.0	41.0
16	38.6023041082924	40.0	38.0	41.0	34.0	41.0
17	38.53150429852091	40.0	38.0	41.0	34.0	41.0
18	38.24795814513424	39.0	38.0	41.0	34.0	41.0
19	38.00026535497833	39.0	37.0	41.0	34.0	41.0
20	37.66670495742833	39.0	35.0	41.0	34.0	41.0
21	37.542353985838545	39.0	35.0	41.0	33.0	41.0
22	37.58536906167723	39.0	35.0	41.0	33.0	41.0
23	37.499998276915726	39.0	35.0	41.0	33.0	41.0
24	37.42870509068018	39.0	35.0	41.0	33.0	41.0
25	37.341081660409955	39.0	35.0	41.0	33.0	41.0
26	37.32212543594032	39.0	35.0	41.0	33.0	41.0
27	37.29133736611635	39.0	35.0	41.0	33.0	41.0
28	37.19010673932721	39.0	35.0	41.0	33.0	41.0
29	37.09251698961095	39.0	35.0	41.0	33.0	41.0
30	36.88007046265962	39.0	35.0	41.0	32.0	41.0
31	36.554845198108744	39.0	35.0	41.0	31.0	41.0
32	36.06328429052579	39.0	35.0	41.0	30.0	41.0
33	35.3567668965644	39.0	35.0	41.0	23.0	41.0
34	34.7183877215312	39.0	35.0	41.0	18.0	41.0
35	34.28720138949516	38.0	34.0	40.0	15.0	41.0
36	34.019722996971964	38.0	34.0	40.0	15.0	41.0
37	33.93099851585008	38.0	34.0	40.0	12.0	41.0
38	33.79438550219866	38.0	33.0	40.0	11.0	41.0
39	33.714631397811914	38.0	33.0	40.0	10.0	41.0
40	33.61348979704365	38.0	33.0	40.0	10.0	41.0
41	33.5115595980389	38.0	33.0	40.0	10.0	41.0
42	33.41538909540373	38.0	33.0	40.0	10.0	41.0
43	33.28717841503816	38.0	33.0	40.0	10.0	41.0
44	33.22676995216718	38.0	33.0	40.0	10.0	41.0
45	33.26755937748411	38.0	33.0	40.0	10.0	41.0
46	33.102435062697296	38.0	32.0	40.0	10.0	41.0
47	33.05996792765803	38.0	32.0	40.0	10.0	41.0
48	32.96144713807189	38.0	32.0	40.0	10.0	41.0
49	32.993381058938674	38.0	32.0	40.0	10.0	41.0
50	32.80409852825628	37.0	32.0	40.0	10.0	41.0
51	31.930400031245263	36.0	30.0	40.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	7.0
11	10.0
12	9.0
13	11.0
14	21.0
15	40.0
16	69.0
17	231.0
18	530.0
19	1299.0
20	2457.0
21	4055.0
22	6463.0
23	11267.0
24	20596.0
25	39353.0
26	56587.0
27	55520.0
28	44741.0
29	36957.0
30	35094.0
31	37901.0
32	44910.0
33	57446.0
34	86054.0
35	112922.0
36	132755.0
37	196259.0
38	335704.0
39	421630.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.120340205759238	3.686251625442832	49.229206967693315	26.964201201104608
2	32.62671561757638	3.305105383834253	44.70094149324781	19.36723750534156
3	15.676390988498987	3.476207652332137	60.83475392059109	20.012647438577787
4	14.42416820978436	3.5639126419247082	60.16217669195388	21.849742456337047
5	14.342034526013977	6.489077943142814	59.046249879384106	20.122637651459108
6	17.4128004484614	5.877785078549669	61.16771123864487	15.541703234344057
7	70.86402337880745	0.9349455275624561	23.85495306318435	4.34607803044575
8	73.40712346013703	1.1754306561964407	21.59288802709148	3.82455785657506
9	67.9963516562286	3.8816493822168514	23.57529648536757	4.546702476186976
10	30.422718521547743	25.729094392854023	30.212042750869582	13.636144334728648
11	20.254683343059188	22.255643675361732	40.70780855844472	16.78186442313436
12	17.67493900281667	20.416883009470073	43.6851258770499	18.223052110663364
13	18.00341630175571	20.413436840920266	45.246182793969666	16.336964063354362
14	16.727817013044895	22.756314529506096	43.7295814513424	16.78628700610661
15	15.361583491474178	22.510315531192422	44.910123924221054	17.21797705311235
16	16.87066069943437	22.583948665873283	43.079634062848925	17.465756571843425
17	16.92327220596141	22.16971920618656	41.751365831468576	19.155642756383454
18	18.087789995083465	21.285489792448757	41.85268318683288	18.7740370256349
19	18.1530374529598	23.652720405453216	40.30006938286014	17.89417275872685
20	19.069833159493275	23.667883547072364	39.384364963034095	17.87791833040026
21	18.870874361884457	24.585311051173306	39.930984731175876	16.61282985576636
22	18.56600331751159	22.476083590264345	39.39361218197608	19.564300910247987
23	16.824998966149433	23.920947190913143	39.072486709276625	20.181567133660796
24	16.81069736666774	23.096681109941965	40.602355800820646	19.490265722569646
25	17.238539192126197	24.040644111876418	39.09052165802061	19.63029503797678
26	17.3149292616469	24.274294339553286	39.2954538144491	19.115322584350718
27	17.861146976791204	22.86096318113521	40.223679313339424	19.054210528734153
28	17.159219879338153	21.992988195723996	40.867768215298234	19.98002370963962
29	17.397637306842253	21.109735196408632	40.66737351412699	20.825253982622122
30	18.85444762513038	21.934173585807297	40.222473154347	18.988905634715323
31	18.829807519999264	23.834965285595473	38.53896238162411	18.79626481278115
32	17.870853684873158	24.37877068275491	38.392327909829845	19.358047722542075
33	18.96518450786416	24.899716495200636	35.897876241195036	20.237222755740166
34	17.855575670969017	25.891064314694923	35.49794838099001	20.755411633346046
35	18.038567220963735	26.624466418236203	33.89708821731998	21.43987814348008
36	19.454827622649137	27.917296549696047	32.896493178883716	19.731382648771095
37	19.39876994757229	27.822871531431353	33.238927460449474	19.53943106054688
38	18.483237836173743	28.221018871218977	33.13628907380774	20.15945421879954
39	19.60370210400077	27.87404713439598	31.668221271590248	20.854029490013005
40	19.871699144890712	28.107582489787852	32.18491680949121	19.83580155583023
41	19.309341873704817	27.862100416756654	32.00755400146117	20.82100370807736
42	20.96602996788171	27.162757945715953	31.83220145841853	20.039010627983807
43	20.788150234569205	25.25030670900093	33.24886391310141	20.712679143328447
44	20.136364889515836	25.150655001769035	32.11817601190996	22.59480409680517
45	19.954522062371055	24.707420290121444	31.930704442800494	23.407353204707007
46	20.07525283389927	25.256050323250612	32.13540685465899	22.53328998819113
47	19.12887751397996	25.880955553615493	33.19602266200438	21.79414427040017
48	19.06242389711119	24.916143231954713	33.665505690773	22.355927180161096
49	20.023617741794673	23.993833655741547	33.41755386361444	22.564994738849347
50	18.816080281942536	24.0106624454931	33.414682056489596	23.758575216074767
51	18.120126543309148	24.021230695712507	32.68116508066331	25.177477680315025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	576.5
2	1030.0
3	28544.5
4	56059.0
5	39725.0
6	23391.0
7	23488.5
8	23586.0
9	24029.0
10	24472.0
11	24598.5
12	24725.0
13	24163.0
14	23601.0
15	22897.5
16	22194.0
17	21385.0
18	20576.0
19	19539.0
20	18502.0
21	18218.0
22	17934.0
23	17365.0
24	16796.0
25	17308.0
26	19017.0
27	20214.0
28	21889.5
29	23565.0
30	26519.5
31	29474.0
32	33415.5
33	37357.0
34	43411.0
35	49465.0
36	54386.5
37	59308.0
38	64973.5
39	70639.0
40	81753.5
41	92868.0
42	105865.0
43	118862.0
44	140665.5
45	162469.0
46	176049.0
47	189629.0
48	178264.5
49	166900.0
50	143132.5
51	119365.0
52	103704.0
53	88043.0
54	75770.5
55	63498.0
56	55437.0
57	47376.0
58	41593.0
59	35810.0
60	31474.0
61	27138.0
62	22978.0
63	18818.0
64	14930.0
65	11042.0
66	8904.0
67	6766.0
68	5628.5
69	4491.0
70	3508.0
71	2525.0
72	2006.0
73	1487.0
74	1397.0
75	1157.5
76	1008.0
77	728.5
78	449.0
79	321.0
80	193.0
81	143.5
82	94.0
83	75.5
84	57.0
85	46.0
86	35.0
87	18.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1741064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.83841773962947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7185751263432	32.51622212264723
2	9.034750350657994	7.559993186881858
3	3.410798907726037	4.281072885819415
4	1.8635407963358899	3.118703932477709
5	1.2064548683682417	2.523808138340009
6	0.9000350705831692	2.2593625958025316
7	0.688633298381992	2.016792932498728
8	0.5738684849407025	1.9207799520445898
9	0.4838092047565984	1.821763045339604
>10	3.965240986416642	30.0123476423005
>50	0.10160875995967533	2.952512775899156
>100	0.04709443869765866	3.541697692617072
>500	0.003074338757742972	0.8132052960796334
>1k	0.0022358827329039795	2.484400937976264
>5k	1.3974267080649872E-4	0.3125014240812165
>10k+	1.3974267080649872E-4	1.8648354391944775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31896	1.8319832010770425	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	5345	0.30699618164524684	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	4684	0.26903089145488046	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	4180	0.24008307563650735	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	3988	0.22905533627712707	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3892	0.22354146659743696	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3579	0.20556395399594732	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	2869	0.16478429282323911	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	2830	0.162544283265865	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2797	0.16064889056347154	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	2578	0.14807037535667844	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGT	2212	0.12704874720285986	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	1946	0.11177073329871848	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1487228499354418E-4	0.0	0.0	0.3253183111017171	0.0
2	1.1487228499354418E-4	0.0	0.0	0.9832493234022414	0.0
3	1.1487228499354418E-4	0.0	0.0	1.6596747735867263	0.0
4	1.1487228499354418E-4	0.0	0.0	2.376937321086416	0.0
5	1.1487228499354418E-4	0.0	0.0	3.58131579310123	0.0
6	1.7230842749031626E-4	0.0	0.0	5.283321003708077	0.0
7	1.7230842749031626E-4	0.0	0.0	6.6481186217163755	0.0
8	1.7230842749031626E-4	0.0	0.0	8.34501201564101	0.0
9	1.7230842749031626E-4	0.0	0.0	9.458469074083434	0.0
10	1.7230842749031626E-4	0.0	0.0	10.790642963153566	0.0
11	1.7230842749031626E-4	0.0	0.0	12.642499069534491	0.0
12	1.7230842749031626E-4	0.0	0.0	14.172540469506004	0.0
13	1.7230842749031626E-4	0.0	0.0	14.869413186419338	0.0
14	1.7230842749031626E-4	0.0	0.0	15.195420731231017	0.0
15	1.7230842749031626E-4	0.0	0.0	15.47634090418273	0.0
16	1.7230842749031626E-4	0.0	0.0	15.991945155376253	0.0
17	1.7230842749031626E-4	0.0	0.0	16.751653012181055	0.0
18	1.7230842749031626E-4	0.0	0.0	17.609232055800362	0.0
19	1.7230842749031626E-4	0.0	0.0	18.185431437327978	0.0
20	2.2974456998708836E-4	0.0	0.0	18.78219295786944	0.0
21	2.2974456998708836E-4	0.0	0.0	19.54569160007903	0.0
22	2.2974456998708836E-4	0.0	0.0	20.362720727095613	0.0
23	2.2974456998708836E-4	0.0	0.0	21.208065872363107	0.0
24	2.2974456998708836E-4	0.0	0.0	21.87047690377838	0.0
25	2.2974456998708836E-4	0.0	0.0	22.45356862240561	0.0
26	2.2974456998708836E-4	0.0	0.0	22.9839913983633	0.0
27	2.2974456998708836E-4	0.0	0.0	23.530726038790075	0.0
28	2.2974456998708836E-4	0.0	0.0	24.093140746118465	0.0
29	2.2974456998708836E-4	0.0	0.0	24.71494442478852	0.0
30	2.2974456998708836E-4	0.0	0.0	25.405671474454703	0.0
31	2.2974456998708836E-4	0.0	0.0	26.002777611851144	0.0
32	2.2974456998708836E-4	0.0	0.0	26.578172887383808	0.0
33	2.2974456998708836E-4	0.0	0.0	27.135705522599974	0.0
34	2.2974456998708836E-4	0.0	0.0	27.689102755556373	0.0
35	2.8718071248386043E-4	0.0	0.0	28.272022165756113	0.0
36	2.8718071248386043E-4	0.0	0.0	28.82369631443761	0.0
37	4.020529974774046E-4	0.0	0.0	29.397827994835342	0.0
38	4.020529974774046E-4	0.0	0.0	29.956337044473955	0.0
39	4.020529974774046E-4	0.0	0.0	30.52989436344672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAC	20	7.0342876E-4	45.000004	20
ACGTGCG	20	7.0342876E-4	45.000004	1
CGATCGG	30	2.166149E-6	45.000004	10
TAGTGCG	45	3.8562575E-10	45.0	1
CGTTTTT	27945	0.0	44.412235	1
CGTTATT	800	0.0	41.625004	1
TACGGGA	320	0.0	40.07813	4
GCGATGC	335	0.0	39.626865	9
GTTTTTT	31885	0.0	39.05833	2
CGTTTGG	1025	0.0	38.414635	2
GTCTTCG	195	0.0	38.076923	1
TTGTTCG	260	0.0	38.07692	1
GCCGATC	95	0.0	37.894737	9
GACCGAT	835	0.0	37.724552	9
ACTAGCG	30	1.14016315E-4	37.500004	20
TATCGCG	30	1.14016315E-4	37.500004	1
ACGACGA	30	1.14016315E-4	37.500004	22
CTAGCGG	90	0.0	37.5	2
GACACGA	230	0.0	37.173916	25
CGTTTCT	585	0.0	36.923077	1
>>END_MODULE