##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535114
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16355580306252	33.0	31.0	34.0	30.0	34.0
2	32.347843637056776	34.0	31.0	34.0	30.0	34.0
3	32.41607395807249	34.0	31.0	34.0	30.0	34.0
4	35.930741860612876	37.0	35.0	37.0	35.0	37.0
5	35.16511248070505	37.0	35.0	37.0	35.0	37.0
6	34.81525992592233	37.0	35.0	37.0	32.0	37.0
7	35.64149321452999	37.0	35.0	37.0	33.0	37.0
8	35.88985150827674	37.0	35.0	37.0	35.0	37.0
9	37.76305609645795	39.0	38.0	39.0	35.0	39.0
10	37.27950119040055	39.0	37.0	39.0	34.0	39.0
11	37.16992640820461	39.0	37.0	39.0	34.0	39.0
12	36.76940053895058	39.0	35.0	39.0	33.0	39.0
13	36.50046719016883	39.0	35.0	39.0	33.0	39.0
14	37.518341886028026	39.0	36.0	41.0	33.0	41.0
15	37.693612949763974	39.0	36.0	41.0	33.0	41.0
16	37.7814185388534	39.0	36.0	41.0	33.0	41.0
17	37.767367327335855	39.0	35.0	41.0	33.0	41.0
18	37.769357557455045	39.0	36.0	41.0	33.0	41.0
19	37.76918002519089	39.0	36.0	41.0	33.0	41.0
20	37.63800610710989	39.0	35.0	41.0	33.0	41.0
21	37.53126997237972	39.0	35.0	41.0	33.0	41.0
22	37.56843775345067	39.0	35.0	41.0	33.0	41.0
23	37.49395269045475	39.0	35.0	41.0	33.0	41.0
24	37.43856075527832	39.0	35.0	41.0	33.0	41.0
25	37.337567322103325	39.0	35.0	41.0	33.0	41.0
26	37.32155764939807	39.0	35.0	41.0	33.0	41.0
27	37.28040006428537	39.0	35.0	41.0	33.0	41.0
28	37.221537840534914	39.0	35.0	41.0	33.0	41.0
29	37.2215920345945	39.0	35.0	41.0	33.0	41.0
30	37.1836337677579	39.0	35.0	41.0	33.0	41.0
31	37.08068000463452	39.0	35.0	41.0	32.0	41.0
32	37.02617199325751	39.0	35.0	41.0	32.0	41.0
33	36.93886723202906	39.0	35.0	41.0	32.0	41.0
34	36.83848862111625	39.0	35.0	41.0	32.0	41.0
35	36.74121775920645	39.0	35.0	41.0	31.0	41.0
36	36.62881741086946	39.0	35.0	40.0	31.0	41.0
37	36.58457823940319	39.0	35.0	40.0	31.0	41.0
38	36.51021838337252	38.0	35.0	40.0	31.0	41.0
39	36.48860429740205	38.0	35.0	40.0	31.0	41.0
40	36.380879588274645	38.0	35.0	40.0	31.0	41.0
41	36.272551269449124	38.0	35.0	40.0	30.0	41.0
42	36.22895495165516	38.0	35.0	40.0	30.0	41.0
43	36.116634960027206	38.0	35.0	40.0	30.0	41.0
44	36.036149306502914	38.0	35.0	40.0	30.0	41.0
45	36.02964415059221	38.0	35.0	40.0	30.0	41.0
46	35.83799900581932	38.0	35.0	40.0	30.0	41.0
47	35.74867037677953	38.0	35.0	40.0	30.0	41.0
48	35.71164649028058	37.0	35.0	40.0	30.0	41.0
49	35.81137290371771	37.0	35.0	40.0	30.0	41.0
50	35.59705595443214	37.0	34.0	40.0	29.0	41.0
51	34.50533904924932	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	11.0
11	8.0
12	4.0
13	10.0
14	9.0
15	9.0
16	20.0
17	35.0
18	99.0
19	180.0
20	356.0
21	595.0
22	989.0
23	1415.0
24	2035.0
25	2970.0
26	3900.0
27	4826.0
28	5556.0
29	6968.0
30	8885.0
31	11720.0
32	16157.0
33	23284.0
34	43357.0
35	60660.0
36	45264.0
37	63595.0
38	104134.0
39	128014.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.03821615580979	4.397380745037506	53.49850686022044	17.065896238932265
2	19.28262015196762	6.146540737113961	52.985345178784335	21.585493932134085
3	19.436232279476897	6.438067402460036	52.362860997843455	21.76283932021962
4	18.200420844904077	4.589489342457869	51.77644389793576	25.433645914702286
5	18.292363870128607	7.56362195719043	50.030647675074846	24.11336649760612
6	20.86676857641549	8.350370201489776	52.64859450509611	18.13426671699862
7	82.34563102441723	1.932485414322929	11.372903717712488	4.348979843547356
8	83.72197326177225	3.6799635217916182	7.734052930777367	4.864010285658757
9	77.7912743826549	5.385955142268751	9.25447661619767	7.568293858878669
10	43.24984956476564	24.283797471193054	17.705012389883276	14.76134057415803
11	35.72005217579806	22.6510612691875	23.003883284683262	18.62500327033118
12	30.381563554681808	20.260355737282147	29.815329070067314	19.542751637968735
13	23.83903243047276	27.879666762596383	30.117694547330103	18.16360625960076
14	18.767589709856217	30.791756522909136	29.955859872849523	20.484793894385124
15	17.11205462761206	23.101058839798622	39.47345799212878	20.31342854046054
16	18.599588125147164	20.743056619710938	35.74266417996913	24.91469107517277
17	18.531565236566415	19.681974308278235	31.287164977929937	30.499295477225413
18	21.18539227155335	20.726050897565752	31.852465082206784	26.236091748674113
19	23.555541436030452	23.607679858871194	29.29020732030932	23.546571384789036
20	26.895764267053373	23.220659523017524	28.88487312983775	20.998703080091342
21	24.293328150637063	26.352702414812544	27.06283147142478	22.291137963125614
22	22.704320948433416	23.60805361100625	27.453776204696567	26.233849235863758
23	20.6694274491043	26.64815347757674	26.701973785025245	25.98044528829371
24	21.739666687845954	23.1528235105043	30.783907728072897	24.323602073576843
25	19.317005348393053	24.944217493842434	30.124235209693634	25.61454194807088
26	19.084905272521368	26.342984859301012	29.35318455506677	25.21892531311085
27	19.24468431025912	25.34282414588293	30.968541282792078	24.443950261065865
28	17.07953819186192	24.250159779037737	35.155686451858855	23.514615577241486
29	17.49122616862949	21.378061497176304	34.84248216267935	26.288230171514854
30	20.33566679249655	24.208112663843593	31.953004406537673	23.50321613712218
31	23.448087697200968	24.609148704761978	27.411355337367365	24.53140826066969
32	24.910766677754648	24.817141767922347	27.54908299913663	22.72300855518637
33	22.94520419947899	23.79081840505014	26.65731040488569	26.606666990585182
34	19.855395298945645	22.31206808268892	30.927615424003108	26.904921194362323
35	19.502199531314822	21.50812724017686	31.346217815269267	27.64345541323905
36	22.979776271971954	20.91685136251341	31.061231812286728	25.04214055322791
37	20.448166185149333	23.334093296007953	32.623328860766115	23.5944116580766
38	19.666837346808343	25.025134831082724	27.704190135186145	27.603837686922787
39	19.82437387173574	23.542086359168326	30.682247147336827	25.9512926217591
40	22.744686179019798	21.956256050112685	29.077355479393173	26.22170229147434
41	18.878407217901234	21.429452415746926	29.47035584940779	30.221784516944055
42	21.727332867389006	21.078686036993986	29.56641014811797	27.627570947499038
43	21.21828245943855	20.1512201138449	29.57444581902174	29.056051607694812
44	20.261663869754855	22.619852965910066	30.164974192415073	26.953508971920005
45	20.282220237183104	23.909484707931394	28.036082031118603	27.772213023766902
46	23.046304152012468	23.290924924408632	29.4634414349092	24.199329488669704
47	18.723113205784188	21.923178986159957	33.25422246474583	26.099485343310025
48	21.098121148017057	20.740627230833056	31.078798162634502	27.08245345851538
49	21.121293780390722	18.910736777583843	32.72199942442171	27.245970017603728
50	20.915169477905643	20.73558157700976	30.77904895031713	27.57019999476747
51	19.414741531710998	22.099029365705253	27.518995952264376	30.967233150319373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	242.0
2	444.0
3	1289.5
4	2135.0
5	1498.5
6	862.0
7	828.0
8	794.0
9	888.0
10	982.0
11	1146.5
12	1311.0
13	1171.5
14	1032.0
15	1004.5
16	977.0
17	1018.0
18	1059.0
19	1081.5
20	1104.0
21	1117.0
22	1130.0
23	1512.0
24	1894.0
25	2281.0
26	2702.0
27	2736.0
28	3282.5
29	3829.0
30	4926.5
31	6024.0
32	6661.0
33	7298.0
34	8428.0
35	9558.0
36	10860.0
37	12162.0
38	14650.5
39	17139.0
40	21137.5
41	25136.0
42	30845.5
43	36555.0
44	39598.0
45	42641.0
46	50626.0
47	58611.0
48	60663.0
49	62715.0
50	60346.0
51	57977.0
52	49389.5
53	40802.0
54	34815.0
55	28828.0
56	26221.5
57	23615.0
58	22254.5
59	20894.0
60	18287.5
61	15681.0
62	14926.5
63	14172.0
64	12415.5
65	10659.0
66	8981.5
67	7304.0
68	6402.0
69	5500.0
70	4307.0
71	3114.0
72	2760.5
73	2407.0
74	1900.0
75	1065.0
76	737.0
77	692.5
78	648.0
79	526.0
80	404.0
81	254.5
82	105.0
83	61.0
84	17.0
85	12.5
86	8.0
87	7.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	535114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.120458850740718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.6284132378751	19.42595433761643
2	12.086407697160157	6.5557784520821585
3	5.049227793155647	4.108121237768823
4	2.5186317517890155	2.732257951382521
5	1.433142294236194	1.9433738309044424
6	0.9357506133220493	1.522679160189362
7	0.6087108321200937	1.15559619521592
8	0.4379343289840826	0.9501583958831649
9	0.343021210465728	0.8372603361030344
>10	3.210209521413147	21.362661715195806
>50	1.581923308093099	29.535961459645726
>100	0.15902201086600207	6.741390629505467
>500	0.0020742001417304616	0.35308295937246315
>1k	0.005531200377947899	2.7757233391346636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2384	0.4455125449904133	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC	2053	0.3836565666381369	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1972	0.3685196051682445	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	1959	0.3660902162903606	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG	1905	0.35599890864376565	No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1867	0.3488976180776433	TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	1492	0.27881909275406735	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1171	0.21883187507708637	No Hit
TCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	801	0.14968773009115816	No Hit
GTTCTGGGAGCTTTGTACATCAGCACCAAGAAGGAAGACGCTTGGGAGAGA	550	0.10278183714124466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8687606752953577E-4	0.0	0.0	0.3879547161913162	0.0
2	1.8687606752953577E-4	0.0	0.0	1.659272603594748	0.0
3	1.8687606752953577E-4	0.0	0.0	2.3224957672570703	0.0
4	1.8687606752953577E-4	0.0	0.0	3.0821469817646334	0.0
5	1.8687606752953577E-4	0.0	0.0	4.506890120609814	0.0
6	1.8687606752953577E-4	0.0	0.0	5.300926531542811	0.0
7	1.8687606752953577E-4	0.0	0.0	6.1409344550880745	0.0
8	1.8687606752953577E-4	0.0	0.0	7.471678931965899	0.0
9	1.8687606752953577E-4	0.0	0.0	8.249830877158885	0.0
10	3.7375213505907155E-4	0.0	0.0	9.870046382639961	0.0
11	3.7375213505907155E-4	0.0	0.0	11.691527412850347	0.0
12	3.7375213505907155E-4	0.0	0.0	13.87106298844732	0.0
13	3.7375213505907155E-4	0.0	0.0	14.484763994214317	0.0
14	3.7375213505907155E-4	0.0	0.0	14.674069450621737	0.0
15	3.7375213505907155E-4	0.0	0.0	15.085570551321775	0.0
16	3.7375213505907155E-4	0.0	0.0	15.820741000982968	0.0
17	3.7375213505907155E-4	0.0	0.0	16.973766337640203	0.0
18	3.7375213505907155E-4	0.0	0.0	18.36449803219501	0.0
19	3.7375213505907155E-4	0.0	0.0	19.194975276296265	0.0
20	3.7375213505907155E-4	0.0	0.0	19.969950328341252	0.0
21	3.7375213505907155E-4	0.0	0.0	20.941892755562368	0.0
22	3.7375213505907155E-4	0.0	0.0	21.848989187350732	0.0
23	3.7375213505907155E-4	0.0	0.0	22.793834584780065	0.0
24	3.7375213505907155E-4	0.0	0.0	23.46845718856169	0.0
25	3.7375213505907155E-4	0.0	0.0	24.05263177565902	0.0
26	3.7375213505907155E-4	0.0	0.0	24.638488247364112	0.0
27	3.7375213505907155E-4	0.0	0.0	25.1673475184727	0.0
28	3.7375213505907155E-4	0.0	0.0	25.69078738362293	0.0
29	3.7375213505907155E-4	0.0	0.0	26.299255859499098	0.0
30	3.7375213505907155E-4	0.0	0.0	27.00658177509839	0.0
31	3.7375213505907155E-4	0.0	0.0	27.598605157031958	0.0
32	3.7375213505907155E-4	0.0	0.0	28.176799709968343	0.0
33	3.7375213505907155E-4	0.0	0.0	28.69706268197057	0.0
34	3.7375213505907155E-4	0.0	0.0	29.243114551291875	0.0
35	3.7375213505907155E-4	0.0	0.0	29.90484270641396	0.0
36	3.7375213505907155E-4	0.0	0.0	30.47163781923104	0.0
37	3.7375213505907155E-4	0.0	0.0	30.99638581685398	0.0
38	3.7375213505907155E-4	0.0	0.0	31.473480417256884	0.0
39	5.606282025886073E-4	0.0	0.0	32.05466498727374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	45	3.8380676E-10	45.000004	5
CGTTAGG	45	3.8380676E-10	45.000004	2
AATGCGA	90	0.0	45.000004	25
TGCGAAT	90	0.0	45.000004	27
ATGCGAA	90	0.0	45.000004	26
GGTACGT	110	0.0	45.0	11
ACAAACG	20	7.0299034E-4	45.0	31
AGATCAA	20	7.0299034E-4	45.0	25
CCCTCGT	20	7.0299034E-4	45.0	31
CGAGTGA	20	7.0299034E-4	45.0	20
GGTCGAC	20	7.0299034E-4	45.0	8
CGTTGAA	20	7.0299034E-4	45.0	27
TAGCGGC	20	7.0299034E-4	45.0	20
GCTATGC	20	7.0299034E-4	45.0	28
ACTCTAT	20	7.0299034E-4	45.0	24
TTCGTGA	20	7.0299034E-4	45.0	20
CGTCTCA	20	7.0299034E-4	45.0	35
ATTCGAC	35	1.210301E-7	45.0	26
GCGTTTC	20	7.0299034E-4	45.0	10
CGCGAAT	40	6.8030204E-9	45.0	16
>>END_MODULE