##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933167.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1168267
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26673525829284	33.0	31.0	34.0	30.0	34.0
2	32.45211240238747	34.0	31.0	34.0	31.0	34.0
3	32.52911449180709	34.0	31.0	34.0	31.0	34.0
4	35.98440510602457	37.0	35.0	37.0	35.0	37.0
5	35.17622341468174	37.0	35.0	37.0	35.0	37.0
6	34.879313547331215	37.0	35.0	37.0	32.0	37.0
7	35.67327246254495	37.0	35.0	37.0	35.0	37.0
8	35.957665499410666	37.0	35.0	37.0	35.0	37.0
9	37.84888214765974	39.0	38.0	39.0	35.0	39.0
10	37.331115233076	39.0	37.0	39.0	34.0	39.0
11	37.24358044864744	39.0	37.0	39.0	34.0	39.0
12	37.0299178184439	39.0	37.0	39.0	33.0	39.0
13	36.945120421958336	39.0	37.0	39.0	33.0	39.0
14	38.09603369777628	40.0	37.0	41.0	33.0	41.0
15	38.19411744061931	40.0	37.0	41.0	33.0	41.0
16	38.210099232452855	40.0	37.0	41.0	34.0	41.0
17	38.14968153684047	40.0	37.0	41.0	34.0	41.0
18	38.11376936950201	40.0	37.0	41.0	34.0	41.0
19	38.10210165998012	40.0	37.0	41.0	34.0	41.0
20	38.03263295120037	40.0	36.0	41.0	34.0	41.0
21	37.91032700572729	40.0	36.0	41.0	33.0	41.0
22	37.93351947799604	40.0	36.0	41.0	33.0	41.0
23	37.85618184884106	40.0	36.0	41.0	33.0	41.0
24	37.79536099196502	40.0	36.0	41.0	33.0	41.0
25	37.679750433762145	40.0	36.0	41.0	33.0	41.0
26	37.619895109593955	40.0	35.0	41.0	33.0	41.0
27	37.56975588628284	39.0	35.0	41.0	33.0	41.0
28	37.49071059954616	39.0	35.0	41.0	33.0	41.0
29	37.401781442084726	39.0	35.0	41.0	33.0	41.0
30	37.319078601038974	39.0	35.0	41.0	32.0	41.0
31	37.25522761492022	39.0	35.0	41.0	32.0	41.0
32	37.213785033729444	39.0	35.0	41.0	32.0	41.0
33	37.113361928394795	39.0	35.0	41.0	32.0	41.0
34	37.0026089926361	39.0	35.0	41.0	31.0	41.0
35	36.84194280930643	39.0	35.0	41.0	31.0	41.0
36	36.70046145273298	39.0	35.0	41.0	31.0	41.0
37	36.67094936345887	39.0	35.0	41.0	31.0	41.0
38	36.55894928128587	39.0	35.0	41.0	31.0	41.0
39	36.514349887482915	39.0	35.0	41.0	31.0	41.0
40	36.41371193400139	39.0	35.0	41.0	30.0	41.0
41	36.30182141582361	38.0	35.0	40.0	30.0	41.0
42	36.22061309615011	38.0	35.0	40.0	30.0	41.0
43	36.07588419428093	38.0	35.0	40.0	30.0	41.0
44	35.976430901497686	38.0	35.0	40.0	30.0	41.0
45	35.95980713312967	38.0	35.0	40.0	30.0	41.0
46	35.73739650268303	38.0	35.0	40.0	29.0	41.0
47	35.645565611285775	38.0	34.0	40.0	29.0	41.0
48	35.53378465710321	37.0	34.0	40.0	29.0	41.0
49	35.53639878555159	37.0	34.0	40.0	29.0	41.0
50	35.303047163020096	37.0	34.0	40.0	28.0	41.0
51	34.29152496817936	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	26.0
11	12.0
12	14.0
13	23.0
14	25.0
15	42.0
16	74.0
17	115.0
18	244.0
19	481.0
20	877.0
21	1382.0
22	2060.0
23	3045.0
24	4389.0
25	6324.0
26	8817.0
27	11154.0
28	13187.0
29	15817.0
30	19589.0
31	24837.0
32	32308.0
33	45604.0
34	78894.0
35	105622.0
36	104127.0
37	151894.0
38	238495.0
39	298662.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.085932410998513	4.423389516266401	54.00520600171023	17.48547207102486
2	19.88312603197728	4.35593918171103	53.96300674417749	21.797928042134203
3	21.458964431932085	4.561457269613881	51.42018048956275	22.55939780889129
4	19.085705579289666	5.003736303430637	51.259515162201794	24.651042955077905
5	17.176724156378636	7.937483469104237	50.113116265374266	24.772676109142857
6	21.921444327366945	7.423987838396531	52.99601888951755	17.658548944718973
7	85.43406601401907	1.9341469030623992	8.502422819441104	4.1293642634774415
8	87.89086741301432	2.5264772522034775	6.146540131665108	3.436115203117095
9	82.90108339959957	4.579518209450408	7.99740127898845	4.521997111961563
10	41.381122637205365	27.84800049988573	15.287087626373081	15.483789236535825
11	33.00812228711416	24.784488477377174	22.646107439480872	19.561281796027792
12	28.206052212379536	21.585647801401564	29.41955905627737	20.78874092994153
13	24.339898328036313	23.776585318253446	31.40908713504704	20.474429218663197
14	22.18987611564822	25.12516402500456	30.735182967592166	21.94977689175505
15	20.224486354574765	22.935339267479097	34.856158737685824	21.984015640260317
16	22.983958290356572	20.89137157858606	33.054515791338794	23.070154339718574
17	22.357303595838964	21.19267256543239	29.7364386736936	26.71358516503505
18	23.804917882641554	21.49328877730861	31.021846889452497	23.67994645059734
19	25.845290502941538	23.896934519249452	27.597116070213403	22.66065890759561
20	29.06090816568473	23.02889664776973	27.221345805368124	20.68884938117742
21	27.868201361503836	24.284773942942838	26.40261173173598	21.444412963817346
22	26.012974773746073	23.342095599721638	26.047213522251333	24.597716104280956
23	24.98469955926171	24.840725621797073	25.926949918126592	24.24762490081463
24	23.70134566841313	22.81447648525551	29.096687657872728	24.38749018845863
25	24.08961307646283	24.337501615640946	27.50295951182392	24.069925796072305
26	24.032862350815353	25.15683486737193	26.382838854474187	24.42746392733853
27	23.427863664727326	24.705824952686328	27.63854495590477	24.227766426681573
28	22.465926025471916	25.044702966017184	29.067927109128306	23.42144389938259
29	23.57046805225175	24.404951950196317	28.07517459621816	23.94940540133377
30	24.49542784312148	24.806743663905596	27.629386090679613	23.068442402293314
31	25.800266548657113	26.585361051882835	25.12867349672635	22.48569890273371
32	26.03411720094807	26.26908061256545	25.340611349973933	22.356190836512543
33	25.0321202259415	26.188448359835554	25.704226859099844	23.0752045551231
34	23.41031630611838	24.82446221625707	27.62416468153256	24.141056796091988
35	23.74337373220334	25.069440461812242	27.14388063687496	24.04330516910946
36	25.758837662965746	27.18257042268591	25.333250019045305	21.725341895303043
37	25.226853108065196	27.3024916393256	26.12022765343881	21.350427599170395
38	23.018453829475625	28.066443715349315	26.035486750888282	22.879615704286778
39	22.58054023609329	26.917391315512635	27.255841344487173	23.246227103906897
40	23.342352390335428	25.624364978211318	28.612209366523235	22.42107326493002
41	21.254302312741867	25.335475537698144	28.090325242431742	25.319896907128253
42	23.18536772843879	24.93753568319571	26.597601404473465	25.27949518389204
43	23.3447491027308	23.993573386905563	27.638459359033508	25.023218151330134
44	23.2364690605829	24.48061958439295	27.276726981075388	25.006184373948763
45	23.288511958310902	25.04290543172066	25.751647525779635	25.916935084188804
46	23.517483588939857	25.843321774902485	26.92158556220453	23.717609073953128
47	22.432800036293074	24.99907983363392	29.016568986370412	23.551551143702596
48	22.936794414290567	23.649816351912705	29.394907157353583	24.018482076443142
49	22.437679057955073	24.502104399080004	29.457992051474534	23.602224491490386
50	22.040252784680213	25.03289059778287	28.55494505964818	24.371911557888737
51	21.51186329837272	25.01688398285666	27.251047919696443	26.220204799074182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	229.0
1	925.0
2	1621.0
3	3411.0
4	5201.0
5	3707.0
6	2213.0
7	2130.0
8	2047.0
9	2051.5
10	2056.0
11	2204.0
12	2352.0
13	2285.0
14	2218.0
15	2212.5
16	2207.0
17	2243.0
18	2279.0
19	2368.0
20	2457.0
21	2708.0
22	2959.0
23	3336.0
24	3713.0
25	4743.5
26	7021.5
27	8269.0
28	9942.5
29	11616.0
30	13572.5
31	15529.0
32	18109.0
33	20689.0
34	24151.0
35	27613.0
36	28636.5
37	29660.0
38	33825.0
39	37990.0
40	45300.5
41	52611.0
42	60368.0
43	68125.0
44	76918.5
45	85712.0
46	95458.5
47	105205.0
48	104121.0
49	103037.0
50	94356.5
51	85676.0
52	81358.5
53	77041.0
54	71871.5
55	66702.0
56	64404.5
57	62107.0
58	62606.5
59	63106.0
60	59195.5
61	55285.0
62	50653.0
63	46021.0
64	40331.0
65	34641.0
66	30007.5
67	25374.0
68	21287.0
69	17200.0
70	14315.0
71	11430.0
72	9899.5
73	8369.0
74	7189.0
75	4452.5
76	2896.0
77	2205.0
78	1514.0
79	1101.5
80	689.0
81	589.5
82	490.0
83	362.5
84	235.0
85	144.5
86	54.0
87	41.0
88	28.0
89	19.0
90	10.0
91	8.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1168267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.41498943666626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7207054140909	21.51603044478311
2	10.770299365820538	6.120758854190479
3	3.7471413998763143	3.1942494988554087
4	1.7224306505129663	1.9577139495886453
5	0.9371407795408857	1.3314422675661726
6	0.5844869391740931	0.9964914121500751
7	0.4134399728184951	0.8223524722233188
8	0.30060767422107804	0.6833411110058196
9	0.22241564059238517	0.5687944270183786
>10	4.030157300568798	31.074430294540285
>50	1.4667793749160183	26.52985654415746
>100	0.08086675666760391	3.8082948515837183
>500	0.0020584265333339472	0.37726158489540296
>1k	0.0014703046666671052	1.018982287441736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2639	0.22589014326348344	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	2544	0.21775844049348306	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2375	0.20329256925000877	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2339	0.20021108188453496	No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	2298	0.19670161016274532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7119374252632318E-4	0.0	0.0	0.23248110235074687	0.0
2	1.7119374252632318E-4	0.0	0.0	0.9009070700447758	0.0
3	1.7119374252632318E-4	0.0	0.0	1.2765061411475287	0.0
4	1.7119374252632318E-4	0.0	0.0	1.6964443915645995	0.0
5	1.7119374252632318E-4	0.0	0.0	2.384386445906629	0.0
6	1.7119374252632318E-4	0.0	0.0	2.8692927216124398	0.0
7	1.7119374252632318E-4	0.0	0.0	3.2823832223284573	0.0
8	1.7119374252632318E-4	0.0	0.0	4.031013458396068	0.0
9	1.7119374252632318E-4	0.0	0.0	4.414573038526296	0.0
10	1.7119374252632318E-4	0.0	0.0	5.147624644024011	0.0
11	1.7119374252632318E-4	0.0	0.0	6.287518178635534	0.0
12	1.7119374252632318E-4	0.0	0.0	7.352343257149265	0.0
13	1.7119374252632318E-4	0.0	0.0	7.697041857726016	0.0
14	1.7119374252632318E-4	0.0	0.0	7.807205031041705	0.0
15	1.7119374252632318E-4	0.0	0.0	7.998428441443608	0.0
16	2.5679061378948475E-4	0.0	0.0	8.46578735854047	0.0
17	2.5679061378948475E-4	0.0	0.0	9.123856104811656	0.0
18	2.5679061378948475E-4	0.0	0.0	9.869319256642532	0.0
19	2.5679061378948475E-4	0.0	0.0	10.325379386732656	0.0
20	2.5679061378948475E-4	0.0	0.0	10.823809968098047	0.0
21	2.5679061378948475E-4	0.0	0.0	11.475972530252074	0.0
22	2.5679061378948475E-4	0.0	0.0	12.201662804821158	0.0
23	2.5679061378948475E-4	0.0	0.0	12.899362902487189	0.0
24	2.5679061378948475E-4	0.0	0.0	13.473204327435424	0.0
25	2.5679061378948475E-4	0.0	0.0	13.963674399773339	0.0
26	2.5679061378948475E-4	0.0	0.0	14.382071906507674	0.0
27	2.5679061378948475E-4	0.0	0.0	14.800897397598323	0.0
28	2.5679061378948475E-4	0.0	0.0	15.237441441040447	0.0
29	2.5679061378948475E-4	0.0	0.0	15.71455840146131	0.0
30	2.5679061378948475E-4	0.0	0.0	16.267428592950072	0.0
31	2.5679061378948475E-4	0.0	0.0	16.763205671306302	0.0
32	2.5679061378948475E-4	0.0	0.0	17.25350454990169	0.0
33	2.5679061378948475E-4	0.0	0.0	17.730621510322553	0.0
34	2.5679061378948475E-4	0.0	0.0	18.196782071221733	0.0
35	2.5679061378948475E-4	0.0	0.0	18.747255550315124	0.0
36	2.5679061378948475E-4	0.0	0.0	19.233188988476094	0.0
37	2.5679061378948475E-4	0.0	0.0	19.711589901965905	0.0
38	2.5679061378948475E-4	0.0	0.0	20.19367148092003	0.0
39	2.5679061378948475E-4	0.0	0.0	20.70245928370826	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.033335E-4	45.0	19
TGCGCTA	20	7.033335E-4	45.0	1
GAGCGTA	35	1.2119563E-7	45.0	9
TTGACGT	20	7.033335E-4	45.0	23
TCGTCGT	20	7.033335E-4	45.0	31
CGTACTC	25	3.890845E-5	45.0	45
CGTCGTA	20	7.033335E-4	45.0	32
CGTAACG	20	7.033335E-4	45.0	16
ACCCGTT	20	7.033335E-4	45.0	34
TTCGCAC	25	3.890845E-5	45.0	40
CGCCCGA	20	7.033335E-4	45.0	27
AATCGGC	20	7.033335E-4	45.0	32
AGTCGAA	25	3.890845E-5	45.0	25
AACCCGA	70	0.0	41.785713	24
TTACGGG	360	0.0	41.250004	3
AATGCGA	50	1.0822987E-9	40.5	25
GCTACGA	90	0.0	40.000004	10
TATGGGA	650	0.0	39.461536	4
TCGTTTG	545	0.0	39.22018	1
TTGGGAC	2335	0.0	39.122055	5
>>END_MODULE