##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933166.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855029
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17522212696879	33.0	31.0	34.0	30.0	34.0
2	32.35590839608949	34.0	31.0	34.0	30.0	34.0
3	32.43967397597041	34.0	31.0	34.0	30.0	34.0
4	35.932129787410716	37.0	35.0	37.0	35.0	37.0
5	35.15649293766644	37.0	35.0	37.0	35.0	37.0
6	34.857504248393916	37.0	35.0	37.0	32.0	37.0
7	35.662459401961804	37.0	35.0	37.0	35.0	37.0
8	35.93623140267757	37.0	35.0	37.0	35.0	37.0
9	37.78459210155445	39.0	38.0	39.0	35.0	39.0
10	37.27293577176914	39.0	37.0	39.0	34.0	39.0
11	37.18010968049037	39.0	37.0	39.0	34.0	39.0
12	36.921683358108325	39.0	35.0	39.0	33.0	39.0
13	36.80749658783503	39.0	35.0	39.0	33.0	39.0
14	37.91269535887087	40.0	37.0	41.0	33.0	41.0
15	38.02260508123116	40.0	37.0	41.0	33.0	41.0
16	38.060292691826824	40.0	37.0	41.0	33.0	41.0
17	38.02174312216311	40.0	36.0	41.0	33.0	41.0
18	38.00268178038406	40.0	36.0	41.0	33.0	41.0
19	38.002308693623256	40.0	36.0	41.0	34.0	41.0
20	37.90160801563456	40.0	36.0	41.0	33.0	41.0
21	37.769336478645755	40.0	36.0	41.0	33.0	41.0
22	37.78281321452255	40.0	36.0	41.0	33.0	41.0
23	37.73498910563267	39.0	36.0	41.0	33.0	41.0
24	37.66824867928456	39.0	35.0	41.0	33.0	41.0
25	37.556719128824874	39.0	35.0	41.0	33.0	41.0
26	37.51665499064944	39.0	35.0	41.0	33.0	41.0
27	37.44425393758574	39.0	35.0	41.0	33.0	41.0
28	37.387140085307045	39.0	35.0	41.0	33.0	41.0
29	37.31381274787171	39.0	35.0	41.0	32.0	41.0
30	37.25241950857807	39.0	35.0	41.0	32.0	41.0
31	37.177393983128056	39.0	35.0	41.0	32.0	41.0
32	37.13537552527458	39.0	35.0	41.0	32.0	41.0
33	37.003452514476116	39.0	35.0	41.0	32.0	41.0
34	36.8846717479758	39.0	35.0	41.0	31.0	41.0
35	36.73616918256574	39.0	35.0	41.0	31.0	41.0
36	36.61439319602025	39.0	35.0	41.0	31.0	41.0
37	36.56779828520436	39.0	35.0	40.0	31.0	41.0
38	36.49037985846094	39.0	35.0	40.0	31.0	41.0
39	36.467008721341614	39.0	35.0	40.0	31.0	41.0
40	36.364788796637306	38.0	35.0	40.0	30.0	41.0
41	36.21911303593212	38.0	35.0	40.0	30.0	41.0
42	36.119990082207735	38.0	35.0	40.0	30.0	41.0
43	35.97187463817017	38.0	35.0	40.0	30.0	41.0
44	35.91140768324817	38.0	35.0	40.0	30.0	41.0
45	35.90710607476471	38.0	35.0	40.0	30.0	41.0
46	35.66499498847408	38.0	34.0	40.0	29.0	41.0
47	35.56662054737325	37.0	34.0	40.0	29.0	41.0
48	35.49256224057898	37.0	34.0	40.0	29.0	41.0
49	35.54197576924292	37.0	34.0	40.0	29.0	41.0
50	35.32955724308766	37.0	34.0	40.0	28.0	41.0
51	34.24749452942532	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	11.0
11	15.0
12	12.0
13	15.0
14	17.0
15	28.0
16	49.0
17	103.0
18	190.0
19	352.0
20	573.0
21	956.0
22	1395.0
23	2159.0
24	3292.0
25	4718.0
26	6532.0
27	8019.0
28	9725.0
29	11918.0
30	14831.0
31	18638.0
32	24933.0
33	35735.0
34	62385.0
35	80284.0
36	76517.0
37	112755.0
38	175315.0
39	203476.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.90555408062183	4.5522432572462455	51.31170989521993	18.230492766912
2	20.027624794012837	4.51329720980224	52.491669873185586	22.967408122999338
3	20.637545627107386	4.652473775743279	52.36793138010524	22.3420492170441
4	19.320514274954416	5.105557823184944	50.851023766445344	24.72290413541529
5	17.710393448643263	8.047563299022606	49.78357459220681	24.458468660127316
6	21.798909744581763	7.370042419613837	53.24275550887748	17.588292326926926
7	84.52789320596143	1.9276539158320944	9.12986577063468	4.41458710757179
8	86.13661057110343	2.3005067664371617	7.044205518175407	4.518677144283996
9	80.1556438436591	4.743581796640816	9.798848927931099	5.30192543176898
10	42.16313130899654	25.78052908146975	16.58575323176173	15.470586377771983
11	32.70006046578537	24.1612857575591	23.284941212520277	19.85371256413525
12	28.2116746917356	21.479739283696812	29.993953421462898	20.31463260310469
13	23.781181690913407	24.130994387324876	31.521386993891436	20.56643692787028
14	20.1669183150513	26.219110696830167	31.35589553102877	22.25807545708976
15	19.086837990290388	22.752327698826587	37.19932306389608	20.961511246986944
16	20.723624578815457	21.433658975309612	33.73066878433363	24.112047661541304
17	20.445856222420524	20.755085500023977	30.91684609527864	27.88221218227686
18	22.723907610151233	21.2294553751978	31.190637978360968	24.855999036289997
19	24.70442523002144	22.749871641780572	28.544061078630083	24.00164204956791
20	27.670757366124427	23.37277449068979	27.407725352005603	21.54874279118018
21	26.620968411597733	24.211108629064043	27.23603526897918	21.93188769035904
22	23.7763865319188	22.96436729046617	27.370884496315327	25.8883616812997
23	24.326660265324335	24.335081032339254	26.387993857518282	24.95026484481813
24	23.997782531352737	22.596192643758283	28.525231307943944	24.88079351694504
25	22.436198070474802	24.101404747675225	27.643740738618224	25.818656443231752
26	23.193014505940734	25.65421757624595	26.84552219866227	24.307245719151048
27	22.229538413316977	25.252008996186092	27.947940947032208	24.570511643464723
28	21.122675371244718	24.332391065098378	29.678408568598257	24.866524995058647
29	21.527456963448024	24.093100935757736	28.421609091621452	25.957833009172788
30	22.36473850594541	24.22467542036586	28.163957011984387	25.24662906170434
31	23.420141305148714	27.25112247654758	23.929714664648802	25.399021553654904
32	24.271457459337636	27.54725278323893	24.176957740614647	24.004332016808785
33	23.69861139212822	27.9641977055749	24.53975245284078	23.797438449456102
34	22.614086773664987	26.116658031481972	25.99139912213504	25.277856072718002
35	22.510932377732217	27.545966277167206	25.252476816575815	24.69062452852476
36	24.059183957503198	27.812740854403767	24.48688874880267	23.64118643929036
37	21.63330132662167	29.41689697074602	25.402998026967506	23.5468036756648
38	20.460241699404346	29.901441939396207	24.115790224659047	25.522526136540396
39	20.897887673985327	28.02489740114078	25.763219727050195	25.3139951978237
40	23.145764646579238	26.423548207136832	26.394894208266624	24.035792938017305
41	19.90704408856308	27.27930865502807	26.143791614085604	26.669855642323242
42	22.50017250876871	27.221065016508213	25.135170853854078	25.143591620869
43	23.15418541359416	25.784622509879785	25.367326722251526	25.693865354274532
44	21.855165146445323	25.158327963145112	26.4055371221327	26.580969768276862
45	22.348949567792438	25.52299395693012	24.890851655323974	27.237204819953476
46	21.985803990274015	26.042859365003995	27.085280148392627	24.886056496329363
47	20.307732252356352	26.836633611257632	28.110508532459132	24.745125603926883
48	21.372023638964293	25.523578732417263	27.239894787194352	25.86450284142409
49	21.832241947349154	24.22268718370956	28.348512155728052	25.596558713213234
50	20.675205168479664	24.976111921350036	27.416263074117953	26.932419836052347
51	19.779329122170125	25.44042365814493	25.308849173536803	29.471398046148146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	533.5
2	956.0
3	2394.5
4	3833.0
5	2662.0
6	1491.0
7	1453.0
8	1415.0
9	1470.0
10	1525.0
11	1580.0
12	1635.0
13	1556.5
14	1478.0
15	1464.0
16	1450.0
17	1633.0
18	1816.0
19	1864.5
20	1913.0
21	2127.5
22	2342.0
23	2603.5
24	2865.0
25	3170.5
26	4069.0
27	4662.0
28	5578.0
29	6494.0
30	7951.5
31	9409.0
32	11415.0
33	13421.0
34	15624.0
35	17827.0
36	18608.0
37	19389.0
38	22923.0
39	26457.0
40	32183.0
41	37909.0
42	43175.5
43	48442.0
44	57766.0
45	67090.0
46	76588.0
47	86086.0
48	86413.0
49	86740.0
50	77058.5
51	67377.0
52	62726.5
53	58076.0
54	54520.5
55	50965.0
56	49163.0
57	47361.0
58	45993.5
59	44626.0
60	42243.5
61	39861.0
62	35032.0
63	30203.0
64	26334.5
65	22466.0
66	19039.0
67	15612.0
68	13296.0
69	10980.0
70	8962.0
71	6944.0
72	5664.0
73	4384.0
74	3828.5
75	2275.5
76	1278.0
77	1046.5
78	815.0
79	523.5
80	232.0
81	195.5
82	159.0
83	138.0
84	117.0
85	75.5
86	34.0
87	29.0
88	24.0
89	15.5
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	855029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.71489286392992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.946601859869	21.973153519176886
2	11.476983257730472	6.820746558091568
3	4.196209596305729	3.740697557664581
4	1.9709244080822788	2.3426323051627764
5	1.0729891256988233	1.5941878457151184
6	0.72791835659817	1.2978009588001533
7	0.5006316000433376	1.0413350051709915
8	0.3475730609255203	0.8262477014232019
9	0.28573933942658786	0.7641642472264047
>10	4.430583274964194	36.41084818606073
>50	0.967590496747152	17.644524667049613
>100	0.07314314917573927	3.7439830665730782
>500	0.001167177912378818	0.24855839410628744
>1k	0.0019452965206313638	1.551119987778632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	3275	0.3830279440814288	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	3221	0.37671236882023884	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	2514	0.29402511493762196	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	2341	0.2737918830823282	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2066	0.24162923128923114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40279335554700485	0.0
2	0.0	0.0	0.0	1.4612369872834723	0.0
3	0.0	0.0	0.0	2.0206332182885025	0.0
4	0.0	0.0	0.0	2.606695211507446	0.0
5	0.0	0.0	0.0	3.6135616452775285	0.0
6	0.0	0.0	0.0	4.263598076790378	0.0
7	0.0	0.0	0.0	4.8773784281001	0.0
8	0.0	0.0	0.0	5.9420206800003275	0.0
9	0.0	0.0	0.0	6.521299277568363	0.0
10	0.0	0.0	0.0	7.552726281798629	0.0
11	0.0	0.0	0.0	9.173606977073293	0.0
12	0.0	0.0	0.0	10.626657107536703	0.0
13	1.1695509742944391E-4	0.0	0.0	11.124651912391275	0.0
14	1.1695509742944391E-4	0.0	0.0	11.293067252689674	0.0
15	1.1695509742944391E-4	0.0	0.0	11.588028008406733	0.0
16	1.1695509742944391E-4	0.0	0.0	12.230930178976386	0.0
17	1.1695509742944391E-4	0.0	0.0	13.136981318762288	0.0
18	1.1695509742944391E-4	0.0	0.0	14.156011082665032	0.0
19	1.1695509742944391E-4	0.0	0.0	14.758680699718957	0.0
20	1.1695509742944391E-4	0.0	0.0	15.433979432276566	0.0
21	1.1695509742944391E-4	0.0	0.0	16.268571007533076	0.0
22	1.1695509742944391E-4	0.0	0.0	17.154388915463684	0.0
23	2.3391019485888783E-4	0.0	0.0	18.051083647455233	0.0
24	2.3391019485888783E-4	0.0	0.0	18.732230134884315	0.0
25	3.5086529228833173E-4	0.0	0.0	19.37033714645936	0.0
26	3.5086529228833173E-4	0.0	0.0	19.910435786388533	0.0
27	3.5086529228833173E-4	0.0	0.0	20.444569716348802	0.0
28	3.5086529228833173E-4	0.0	0.0	20.974844128093903	0.0
29	4.6782038971777566E-4	0.0	0.0	21.547807150400747	0.0
30	4.6782038971777566E-4	0.0	0.0	22.233514886629575	0.0
31	4.6782038971777566E-4	0.0	0.0	22.810220472054166	0.0
32	4.6782038971777566E-4	0.0	0.0	23.39581464488339	0.0
33	4.6782038971777566E-4	0.0	0.0	23.96819289170309	0.0
34	4.6782038971777566E-4	0.0	0.0	24.51706316393947	0.0
35	4.6782038971777566E-4	0.0	0.0	25.165228313893447	0.0
36	4.6782038971777566E-4	0.0	0.0	25.72836710801622	0.0
37	4.6782038971777566E-4	0.0	0.0	26.342264414423372	0.0
38	5.847754871472195E-4	0.0	0.0	26.917449583581377	0.0
39	5.847754871472195E-4	0.0	0.0	27.497546866831417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	30	2.1648466E-6	45.000004	30
TCGTTCA	80	0.0	45.0	16
TCGCAAT	20	7.032271E-4	45.0	30
ACCGGAT	20	7.032271E-4	45.0	13
GATCGAG	20	7.032271E-4	45.0	9
TTTCGTC	25	3.889964E-5	45.0	16
ATTAACG	20	7.032271E-4	45.0	15
CGTTCTA	25	3.889964E-5	45.0	34
ACTTACG	20	7.032271E-4	45.0	25
CTACGCG	25	3.889964E-5	45.0	1
CGAATAT	50	2.1827873E-11	45.0	14
CATACTA	20	7.032271E-4	45.0	26
CGTAAAG	20	7.032271E-4	45.0	17
CACGTTA	20	7.032271E-4	45.0	10
TCGACGG	25	3.889964E-5	45.0	2
TCGGTCA	25	3.889964E-5	45.0	24
TACGTTG	20	7.032271E-4	45.0	1
ATCGAAA	20	7.032271E-4	45.0	25
GGCTACG	35	1.211447E-7	45.0	1
TACGGGA	155	0.0	43.548386	4
>>END_MODULE