FastQCFastQC Report
Sat 14 Jan 2017
SRR2933165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418002
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382911.5835801624647126No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT44720.18494608358471168Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT35250.14578151713687582No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC33250.13751022538442895Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT32700.1352356201525061No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC32240.1333332230494433Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC30720.1270470413175837Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC27250.11269635012708838No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC26360.10901562529724955No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG25120.10388742441073251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT344600.044.334011
CGTTATT8700.041.379311
GTTTTTT385000.039.7694782
ACACGCG5400.038.75000436
ACTCGCG356.25115E-638.571431
TTGCGCG3100.038.467741
TCTCGCG2500.037.81
GACACGA3400.037.7205925
GCGAGAC3450.037.17391221
CCGTACT1350.036.66666844
TGGGCGA39650.036.3745276
ATGGGCG3300.036.1363645
TACACGC5800.036.07758335
CGTTTAT5150.035.825241
TTTGGGA132500.035.558494
TAGGGCG4050.035.5555535
CACGACC3550.035.49295827
TTGGGCG30000.035.4755
GGCCGTA1400.035.35714342
TTGGGCA38400.035.2734385
Produced by FastQC (version 0.11.5)