##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418002
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17991672463464	33.0	31.0	34.0	30.0	34.0
2	32.429031076070245	34.0	31.0	34.0	31.0	34.0
3	32.37796081227393	34.0	31.0	34.0	30.0	34.0
4	35.96912492214646	37.0	35.0	37.0	35.0	37.0
5	35.17935014114959	37.0	35.0	37.0	35.0	37.0
6	34.96559721621405	37.0	35.0	37.0	33.0	37.0
7	35.765081666599116	37.0	35.0	37.0	35.0	37.0
8	36.03385274288441	37.0	35.0	37.0	35.0	37.0
9	37.90406500904466	39.0	38.0	39.0	35.0	39.0
10	37.40729908411986	39.0	37.0	39.0	34.0	39.0
11	37.293879409529026	39.0	37.0	39.0	34.0	39.0
12	37.29457254377788	39.0	37.0	39.0	34.0	39.0
13	37.36103320013797	39.0	37.0	39.0	34.0	39.0
14	38.621158708719015	40.0	38.0	41.0	34.0	41.0
15	38.67755320301637	40.0	38.0	41.0	34.0	41.0
16	38.63251312447219	40.0	38.0	41.0	34.0	41.0
17	38.56134155389449	40.0	38.0	41.0	34.0	41.0
18	38.31726731408824	39.0	38.0	41.0	34.0	41.0
19	38.09694574280749	39.0	37.0	41.0	34.0	41.0
20	37.79572845680028	39.0	35.0	41.0	34.0	41.0
21	37.66937041408568	39.0	35.0	41.0	33.0	41.0
22	37.710025467307304	39.0	35.0	41.0	33.0	41.0
23	37.61833737110226	39.0	35.0	41.0	33.0	41.0
24	37.544851079527646	39.0	35.0	41.0	33.0	41.0
25	37.44933916514544	39.0	35.0	41.0	33.0	41.0
26	37.42274158582168	39.0	35.0	41.0	33.0	41.0
27	37.38013243992354	39.0	35.0	41.0	33.0	41.0
28	37.26068258008058	39.0	35.0	41.0	33.0	41.0
29	37.16069755111865	39.0	35.0	41.0	33.0	41.0
30	36.96803476589349	39.0	35.0	41.0	32.0	41.0
31	36.67845146530069	39.0	35.0	41.0	31.0	41.0
32	36.26565279929462	39.0	35.0	41.0	30.0	41.0
33	35.64474719210323	39.0	35.0	41.0	25.0	41.0
34	35.11531917674179	39.0	35.0	41.0	21.0	41.0
35	34.746023369707714	39.0	35.0	41.0	18.0	41.0
36	34.524195596198844	39.0	34.0	41.0	17.0	41.0
37	34.437772177194226	39.0	34.0	41.0	16.0	41.0
38	34.34227970034764	39.0	34.0	41.0	15.0	41.0
39	34.26713666903501	39.0	34.0	41.0	15.0	41.0
40	34.2144361336343	39.0	34.0	40.0	15.0	41.0
41	34.107625634718254	38.0	34.0	40.0	15.0	41.0
42	33.98879984383801	38.0	34.0	40.0	14.0	41.0
43	33.85748812449287	38.0	33.0	40.0	12.0	41.0
44	33.804407936800715	38.0	33.0	40.0	12.0	41.0
45	33.84990789916634	38.0	33.0	40.0	12.0	41.0
46	33.684899764350895	38.0	33.0	40.0	10.0	41.0
47	33.636507331259445	38.0	33.0	40.0	10.0	41.0
48	33.524426778803324	38.0	33.0	40.0	10.0	41.0
49	33.56233948524443	38.0	33.0	40.0	10.0	41.0
50	33.38557619059041	38.0	33.0	40.0	10.0	41.0
51	32.54254173487036	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	18.0
11	10.0
12	14.0
13	11.0
14	24.0
15	53.0
16	109.0
17	314.0
18	862.0
19	1658.0
20	3117.0
21	5055.0
22	8392.0
23	14128.0
24	25189.0
25	47997.0
26	69981.0
27	70656.0
28	58856.0
29	49260.0
30	47368.0
31	51163.0
32	60327.0
33	77192.0
34	114726.0
35	150554.0
36	178717.0
37	267406.0
38	465308.0
39	649260.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.06449953308558	3.7933798235071765	49.565550400702726	26.576570242704513
2	30.04298590323747	3.3993354844206083	46.53987052119891	20.017808091143017
3	16.664171493654678	3.6647198802978656	59.187502739865394	20.483605886182062
4	14.657101193464687	3.7425113792296285	59.42422710982043	22.176160317485262
5	14.506894535240253	6.808307023732818	58.24751178865857	20.43728665236836
6	17.893120022233234	6.230764077118216	60.37542566135181	15.500690239296741
7	74.0020479718379	1.124151262075052	21.012803132503613	3.8609976335834295
8	76.6623435381774	1.3066573145927918	18.782201172703743	3.2487979745260755
9	71.05238126353906	4.2766714006026465	20.604532171602834	4.066415164255447
10	31.23248037015685	26.006760953878448	28.531324622560277	14.229434053404422
11	20.828229257047763	23.414372692826557	38.24649441977302	17.510903630352665
12	17.19080463953297	21.679221108998256	42.06270300851695	19.067271242951826
13	18.092127301797102	20.91449883002578	44.08333822718095	16.91003564099616
14	17.585510681959736	23.002834571683564	42.124117349778864	17.287537396577836
15	16.54365877282153	23.229550678618132	43.0161348088215	17.210655739738844
16	17.85995214230592	22.861974473139394	41.622298079157915	17.65577530539677
17	17.991672463463637	22.996961954539326	40.50191025483023	18.50945532716681
18	18.70048081018957	22.081578096296035	40.52002438376809	18.697916709746313
19	18.609744739665228	24.233230576318796	39.07308596105379	18.08393872296218
20	19.671075540880445	24.467556271665615	38.199182630949025	17.662185556504916
21	19.896344171758336	24.595182303405867	38.422507508265085	17.085966016570705
22	19.159041224945224	22.71416648952317	38.45538589298106	19.671406392550544
23	17.185800508022737	24.589888676684303	38.06179647494088	20.162514340352075
24	17.188323252007237	23.96718447710134	39.309893043926344	19.53459922696507
25	17.75833932312711	24.771319461274224	37.974203495282474	19.496137720316195
26	17.831540255136265	25.00725805851277	37.752450163399374	19.408751522951594
27	17.559497469398288	24.23401634903528	38.19810736302121	20.00837881854523
28	17.31743811626293	24.47082343190783	38.654227746709886	19.557510705119352
29	17.637206255412526	23.251428245303355	37.71063878359075	21.40072671569337
30	18.355692013488824	23.991295292559727	37.019282862462475	20.633729831488974
31	17.899819768552714	26.197207446478536	35.3700699999421	20.532902785026646
32	17.549820058047928	26.965941301950952	34.771021694771136	20.713216945229988
33	17.864873560898626	27.26358373566275	33.58777205312485	21.28377065031377
34	17.513881295383545	27.76461723356722	32.5839680860479	22.137533385001333
35	17.556313022073596	28.644889458321376	31.53793917457471	22.260858345030318
36	18.801597351863233	28.991911503795283	30.98913896679986	21.217352177541624
37	18.428355311534066	29.512878814823146	31.796954675802585	20.261811197840203
38	18.68972813091139	29.08000076095884	32.23984099268735	19.99043011544242
39	19.37223376986454	27.442078211680553	32.131611140106585	21.054076878348322
40	20.3218194195042	26.772599857237505	31.88066014833735	21.024920574920948
41	19.534227018836212	26.729465070748493	31.970610446145205	21.765697464270087
42	20.028478057503673	26.715403874769333	32.24728515526456	21.008832912462438
43	19.407924393776348	26.41896077836164	32.884464115414296	21.288650712447716
44	19.579140133052	26.930829668461815	31.566268348826842	21.923761849659346
45	19.654822452586888	26.407215544073164	30.679916724634637	23.25804527870531
46	20.06520259288454	26.285627555312196	30.753779359983984	22.895390491819278
47	19.26412798666006	26.682856341723454	31.668584227804608	22.384431443811874
48	19.002837880200264	26.233807912483115	32.32871602256739	22.43463818474923
49	19.6372873140717	25.194106539200543	32.4188317462103	22.749774400517452
50	19.28224211559792	24.676199606121088	32.56606073940386	23.47549753887714
51	18.581911842918245	24.538027677396464	32.05969225831905	24.820368221366234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	819.0
2	1443.0
3	36021.0
4	70599.0
5	49913.0
6	29227.0
7	29092.0
8	28957.0
9	29399.5
10	29842.0
11	29763.0
12	29684.0
13	29066.5
14	28449.0
15	27662.0
16	26875.0
17	25794.5
18	24714.0
19	23651.0
20	22588.0
21	21846.5
22	21105.0
23	21314.5
24	21524.0
25	22884.5
26	27090.5
27	29936.0
28	33019.5
29	36103.0
30	42209.0
31	48315.0
32	53263.0
33	58211.0
34	65513.0
35	72815.0
36	80778.5
37	88742.0
38	97291.5
39	105841.0
40	118590.0
41	131339.0
42	149766.0
43	168193.0
44	192802.5
45	217412.0
46	229023.5
47	240635.0
48	230471.0
49	220307.0
50	194125.5
51	167944.0
52	149552.0
53	131160.0
54	115482.0
55	99804.0
56	86865.5
57	73927.0
58	65588.5
59	57250.0
60	49644.0
61	42038.0
62	34832.5
63	27627.0
64	22589.0
65	17551.0
66	13780.0
67	10009.0
68	7800.5
69	5592.0
70	4367.0
71	3142.0
72	2426.5
73	1711.0
74	1479.0
75	1036.0
76	825.0
77	645.0
78	465.0
79	374.0
80	283.0
81	178.0
82	73.0
83	51.5
84	30.0
85	19.5
86	9.0
87	10.5
88	12.0
89	8.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2418002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.68771770615412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55143754701334	28.08497530725112
2	8.269774628263189	6.067983141104709
3	3.085446031998449	3.3959391905859735
4	1.7938713029113305	2.6325217584952707
5	1.2545037510908215	2.3012439740665744
6	0.9651285109465354	2.124501741586042
7	0.785322238870169	2.0168176425623585
8	0.6567576066047972	1.9275950137988975
9	0.5967815298727297	1.9705097070195736
>10	5.88203979983245	39.48393783916419
>50	0.10486206403092262	2.645381757194556
>100	0.04915120913540331	3.2527614211861735
>500	0.002977168957562943	0.7681915845455289
>1k	0.0018321039738848828	1.7190418702615653
>5k	0.0	0.0
>10k+	1.1450649836780517E-4	1.6085980511775562
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38291	1.5835801624647126	No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	4472	0.18494608358471168	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	3525	0.14578151713687582	No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	3325	0.13751022538442895	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT	3270	0.1352356201525061	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	3224	0.1333332230494433	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	3072	0.1270470413175837	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	2725	0.11269635012708838	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC	2636	0.10901562529724955	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	2512	0.10388742441073251	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6542583504893708E-4	0.0	0.0	0.20397005461533943	0.0
2	1.6542583504893708E-4	0.0	0.0	0.6605453593504058	0.0
3	1.6542583504893708E-4	0.0	0.0	1.06377083228219	0.0
4	1.6542583504893708E-4	0.0	0.0	1.6011153009799	0.0
5	1.6542583504893708E-4	0.0	0.0	2.531594266671409	0.0
6	1.6542583504893708E-4	0.0	0.0	3.925472352793753	0.0
7	1.6542583504893708E-4	0.0	0.0	5.103138872507136	0.0
8	1.6542583504893708E-4	0.0	0.0	6.560623192205797	0.0
9	1.6542583504893708E-4	0.0	0.0	7.655287299183375	0.0
10	2.0678229381117138E-4	0.0	0.0	8.838412871453373	0.0
11	2.0678229381117138E-4	0.0	0.0	10.259048586394883	0.0
12	2.0678229381117138E-4	0.0	0.0	11.474018631911802	0.0
13	2.0678229381117138E-4	0.0	0.0	12.030304358722615	0.0
14	2.0678229381117138E-4	0.0	0.0	12.293827713955572	0.0
15	2.894952113356399E-4	0.0	0.0	12.534315521657964	0.0
16	2.894952113356399E-4	0.0	0.0	12.937830489801083	0.0
17	3.3085167009787417E-4	0.0	0.0	13.523644728168133	0.0
18	3.3085167009787417E-4	0.0	0.0	14.185844345869027	0.0
19	3.3085167009787417E-4	0.0	0.0	14.66566198042847	0.0
20	3.3085167009787417E-4	0.0	0.0	15.136505263436506	0.0
21	3.3085167009787417E-4	0.0	0.0	15.715040765061403	0.0
22	3.3085167009787417E-4	0.0	0.0	16.31826607256735	0.0
23	3.3085167009787417E-4	0.0	0.0	16.928439265145357	0.0
24	3.3085167009787417E-4	0.0	0.0	17.405775512179062	0.0
25	3.3085167009787417E-4	0.0	0.0	17.842044795661874	0.0
26	3.7220812886010847E-4	0.0	0.0	18.234310807021664	0.0
27	4.1356458762234277E-4	0.0	0.0	18.646345205669807	0.0
28	4.962775051468113E-4	0.0	0.0	19.082449063317565	0.0
29	4.962775051468113E-4	0.0	0.0	19.56272161892339	0.0
30	4.962775051468113E-4	0.0	0.0	20.092208360456276	0.0
31	4.962775051468113E-4	0.0	0.0	20.557675303825224	0.0
32	4.962775051468113E-4	0.0	0.0	21.01648385733345	0.0
33	4.962775051468113E-4	0.0	0.0	21.47884906629523	0.0
34	5.376339639090456E-4	0.0	0.0	21.926863584066513	0.0
35	5.376339639090456E-4	0.0	0.0	22.387450465301516	0.0
36	5.789904226712799E-4	0.0	0.0	22.84026233228922	0.0
37	5.789904226712799E-4	0.0	0.0	23.284099847725518	0.0
38	5.789904226712799E-4	0.0	0.0	23.75870656848092	0.0
39	5.789904226712799E-4	0.0	0.0	24.24030253076714	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34460	0.0	44.33401	1
CGTTATT	870	0.0	41.37931	1
GTTTTTT	38500	0.0	39.769478	2
ACACGCG	540	0.0	38.750004	36
ACTCGCG	35	6.25115E-6	38.57143	1
TTGCGCG	310	0.0	38.46774	1
TCTCGCG	250	0.0	37.8	1
GACACGA	340	0.0	37.72059	25
GCGAGAC	345	0.0	37.173912	21
CCGTACT	135	0.0	36.666668	44
TGGGCGA	3965	0.0	36.374527	6
ATGGGCG	330	0.0	36.136364	5
TACACGC	580	0.0	36.077583	35
CGTTTAT	515	0.0	35.82524	1
TTTGGGA	13250	0.0	35.55849	4
TAGGGCG	405	0.0	35.555553	5
CACGACC	355	0.0	35.492958	27
TTGGGCG	3000	0.0	35.475	5
GGCCGTA	140	0.0	35.357143	42
TTGGGCA	3840	0.0	35.273438	5
>>END_MODULE