##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933164.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884665 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.126185290223994 33.0 31.0 34.0 30.0 34.0 2 32.37571292510871 34.0 31.0 34.0 30.0 34.0 3 32.31649497390783 33.0 31.0 34.0 30.0 34.0 4 35.93807387519798 37.0 35.0 37.0 35.0 37.0 5 35.164557096353995 37.0 35.0 37.0 35.0 37.0 6 34.94775039595896 37.0 35.0 37.0 33.0 37.0 7 35.76449766934707 37.0 35.0 37.0 35.0 37.0 8 36.028968543481206 37.0 35.0 37.0 35.0 37.0 9 37.910597904667405 39.0 38.0 39.0 35.0 39.0 10 37.35306062350603 39.0 37.0 39.0 34.0 39.0 11 37.220066696203304 39.0 37.0 39.0 34.0 39.0 12 37.23208846134459 39.0 37.0 39.0 34.0 39.0 13 37.27924273014037 39.0 37.0 39.0 34.0 39.0 14 38.52871040741989 40.0 38.0 41.0 34.0 41.0 15 38.58344427258956 40.0 38.0 41.0 34.0 41.0 16 38.54586783327541 40.0 38.0 41.0 34.0 41.0 17 38.47439306189694 40.0 38.0 41.0 34.0 41.0 18 38.23371262266769 39.0 38.0 41.0 34.0 41.0 19 38.006835697590816 39.0 37.0 41.0 34.0 41.0 20 37.6852549392067 39.0 35.0 41.0 34.0 41.0 21 37.562011285825335 39.0 35.0 41.0 33.0 41.0 22 37.597731161771456 39.0 35.0 41.0 33.0 41.0 23 37.50623957042764 39.0 35.0 41.0 33.0 41.0 24 37.422698994250965 39.0 35.0 41.0 33.0 41.0 25 37.34471802681113 39.0 35.0 41.0 33.0 41.0 26 37.315976048793814 39.0 35.0 41.0 33.0 41.0 27 37.28921532473941 39.0 35.0 41.0 33.0 41.0 28 37.194336394001056 39.0 35.0 41.0 33.0 41.0 29 37.08946152233951 39.0 35.0 41.0 33.0 41.0 30 36.89863291354166 39.0 35.0 41.0 32.0 41.0 31 36.608086317727555 39.0 35.0 41.0 31.0 41.0 32 36.14386429418491 39.0 35.0 41.0 30.0 41.0 33 35.488839130561665 39.0 35.0 41.0 24.0 41.0 34 34.91249638529924 39.0 35.0 41.0 19.0 41.0 35 34.52037470850257 39.0 34.0 41.0 16.0 41.0 36 34.280861054882436 39.0 34.0 41.0 15.0 41.0 37 34.22395810396012 39.0 34.0 41.0 15.0 41.0 38 34.11349868544277 39.0 34.0 41.0 13.0 41.0 39 34.073504840382775 39.0 34.0 40.0 12.0 41.0 40 33.993119201555714 39.0 34.0 40.0 12.0 41.0 41 33.865050287451616 38.0 33.0 40.0 10.0 41.0 42 33.737136308044136 38.0 33.0 40.0 10.0 41.0 43 33.62177416145575 38.0 33.0 40.0 10.0 41.0 44 33.56221185197369 38.0 33.0 40.0 10.0 41.0 45 33.599936858805144 38.0 33.0 40.0 10.0 41.0 46 33.42770571958412 38.0 33.0 40.0 10.0 41.0 47 33.37471062496518 38.0 33.0 40.0 10.0 41.0 48 33.277358045063714 38.0 33.0 40.0 10.0 41.0 49 33.32667078764661 38.0 33.0 40.0 10.0 41.0 50 33.15473041628088 38.0 33.0 40.0 10.0 41.0 51 32.22206758230242 37.0 31.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 4.0 10 7.0 11 3.0 12 4.0 13 6.0 14 16.0 15 48.0 16 100.0 17 266.0 18 748.0 19 1484.0 20 2660.0 21 4493.0 22 7286.0 23 12477.0 24 22652.0 25 42001.0 26 60484.0 27 57320.0 28 45412.0 29 37194.0 30 35746.0 31 39004.0 32 46407.0 33 60692.0 34 90846.0 35 118196.0 36 139512.0 37 207046.0 38 365256.0 39 487111.0 40 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.918712344103596 3.8622248516314572 51.02344448482887 25.19561831943608 2 31.077936927782922 3.7289385646786037 46.3960438592535 18.797080648284975 3 16.05516099678192 3.830919553342371 60.064679929854904 20.0492395200208 4 14.663348658780206 3.6683442415495593 59.84511836321044 21.823188736459795 5 14.636977924458725 6.724006653702381 58.77904030689805 19.859975114940852 6 17.62090344968469 6.347122698198354 60.83192503707555 15.2000488150414 7 73.5732345005611 0.9704642469616616 21.813956326455898 3.6423449260213356 8 76.50701848869693 1.2556608203579946 19.094374862376075 3.142945828569003 9 71.2071376080099 3.8814325092257773 20.880368659682226 4.031061223082086 10 31.509154146758178 25.468876431620473 29.180995030947145 13.840974390674205 11 20.969190811099057 23.66452393396174 38.56202561197878 16.80425964296042 12 17.748406215428204 22.102707908302005 41.98783338152934 18.161052494740446 13 17.87903951100063 22.352301337372953 44.14163790381845 15.627021247807965 14 17.64324163710792 23.731220137265773 42.32566530391343 16.299872921712875 15 16.16908044665763 23.715143009500363 43.29904784139356 16.816728702448447 16 17.57983514311562 23.24413091981864 41.58818675998121 17.587847177084523 17 17.815420777697895 23.047862617494356 40.55251198488856 18.58420461991919 18 18.705552445660103 21.80297294214091 40.833676011386636 18.657798600812345 19 19.108064297899098 24.348252872526416 38.5805434918142 17.963139337760293 20 20.0037141879326 24.273014036977393 38.33683970360781 17.3864320714822 21 20.126229329880907 25.03484704178196 38.430967837785495 16.407955790551636 22 19.38668145267196 22.458049573796934 39.17927058654986 18.975998386981242 23 17.80889441890203 24.397120973753957 38.20121878424017 19.592765823103843 24 17.048334849960074 24.367460530120738 39.65712739399309 18.92707722592609 25 17.276067630056268 25.6407902730724 37.99184470449655 19.091297392374774 26 17.39720321648675 25.9984135111545 38.142906033698296 18.46147723866045 27 17.837016127534604 24.622572181262985 38.7818524777613 18.758559213441114 28 17.260308861256508 23.957573361844148 39.37437157266676 19.40774620423258 29 17.331302910596843 23.305415020706597 38.9979120957836 20.36536997291296 30 18.640978635460414 23.850286390419516 38.74338410274505 18.76535087137502 31 19.2878309938371 24.056211581368572 37.554101126725435 19.10185629806889 32 18.86950731297074 24.992876718143542 36.66741834755779 19.470197621327927 33 19.443826887006445 24.170396330382324 35.833795395998756 20.551981386612475 34 19.43480671631298 24.947298326227738 34.82534031246933 20.79255464498996 35 19.264909148310178 25.277383513780965 34.233829354288424 21.223877983620433 36 20.541740839884014 25.89935081300921 33.71872454786394 19.840183799242837 37 21.150708481348143 25.572926753560978 33.466478127412564 19.809886637678314 38 20.22417777164642 25.832760729360388 33.46663730689539 20.4764241920978 39 21.231783897934115 24.72725922113479 32.82816840128086 21.21278847965023 40 21.732933969697534 25.14303603027594 33.319820763902335 19.804209236124194 41 20.592359915422634 25.347422486224342 32.84196395645911 21.218253641893916 42 21.26972167467428 26.214791488142453 32.2965619884701 20.218924848713165 43 21.22472694086217 25.445423987817463 32.48757736786113 20.842271703459236 44 20.64913393096386 25.79323115779197 31.25913623906636 22.298498672177814 45 20.384365391196845 24.929629403634067 31.378945329806623 23.307059875362466 46 20.88901741158243 25.161288610973305 31.997251500929874 21.952442476514396 47 20.09375671538443 25.328267888457628 33.03568538705818 21.54229000909976 48 20.066377844338383 24.501383535004894 33.5011792546686 21.93105936598812 49 21.083853098561285 23.54450260391104 33.06656620672639 22.305078090801285 50 20.161991653689117 23.345475190551106 33.13241345278869 23.360119702971087 51 19.523575807902198 23.272517927589252 32.42486065162774 24.779045612880804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 122.0 1 526.0 2 930.0 3 23929.0 4 46928.0 5 34626.5 6 22325.0 7 22544.5 8 22764.0 9 23436.0 10 24108.0 11 24341.5 12 24575.0 13 24016.0 14 23457.0 15 22950.5 16 22444.0 17 21839.0 18 21234.0 19 20493.5 20 19753.0 21 19043.5 22 18334.0 23 18637.0 24 18940.0 25 19598.0 26 21632.5 27 23009.0 28 25130.5 29 27252.0 30 30834.0 31 34416.0 32 39188.5 33 43961.0 34 50046.5 35 56132.0 36 63855.5 37 71579.0 38 79394.0 39 87209.0 40 94584.5 41 101960.0 42 110708.0 43 119456.0 44 131482.5 45 143509.0 46 161384.5 47 179260.0 48 184129.5 49 188999.0 50 176103.0 51 163207.0 52 139965.0 53 116723.0 54 97672.0 55 78621.0 56 66761.5 57 54902.0 58 46996.5 59 39091.0 60 32583.5 61 26076.0 62 21716.5 63 17357.0 64 14084.0 65 10811.0 66 8164.5 67 5518.0 68 4349.0 69 3180.0 70 2610.5 71 2041.0 72 1698.0 73 1355.0 74 1272.5 75 1090.5 76 991.0 77 754.5 78 518.0 79 319.5 80 121.0 81 75.5 82 30.0 83 18.5 84 7.0 85 8.0 86 9.0 87 6.0 88 3.0 89 1.5 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1884665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.31225478756468 #Duplication Level Percentage of deduplicated Percentage of total 1 77.46320864891393 30.452533950683886 2 9.051458969219636 7.116665223942999 3 3.4010530356635185 4.011091904520736 4 1.83216677744595 2.8810642867306604 5 1.1486933609786227 2.257886303978781 6 0.8303827323963293 1.9586530528295187 7 0.6710114426741849 1.8465280959845236 8 0.5527184708185637 1.7382887480490012 9 0.4442259393949057 1.571717098146402 >10 4.4051079954485886 35.160544127012045 >50 0.14399239900345814 3.6967325197702223 >100 0.05087881801588755 3.393761686864006 >500 0.002757518933186531 0.7115657390997655 >1k 0.0022060151465492246 1.8190781360395516 >5k 0.0 0.0 >10k+ 1.3787594665932653E-4 1.3838891263478985 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25532 1.3547235185032884 No Hit CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT 4113 0.21823507095425446 TruSeq Adapter, Index 15 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 3321 0.17621168748822735 TruSeq Adapter, Index 14 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 2926 0.1552530555828224 TruSeq Adapter, Index 14 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 2637 0.1399187654039312 TruSeq Adapter, Index 14 (95% over 21bp) CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT 2528 0.1341352441945916 No Hit CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT 2417 0.1282456033300348 No Hit CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG 2305 0.12230290263786933 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.652991380431005E-4 0.0 0.0 0.23224286544293019 0.0 2 2.652991380431005E-4 0.0 0.0 0.7514332785932778 0.0 3 2.652991380431005E-4 0.0 0.0 1.202813232059809 0.0 4 2.652991380431005E-4 0.0 0.0 1.7848795409263716 0.0 5 2.652991380431005E-4 0.0 0.0 2.7734371891025726 0.0 6 3.183589656517206E-4 0.0 0.0 4.0401875134307685 0.0 7 3.183589656517206E-4 0.0 0.0 5.034528682816309 0.0 8 3.183589656517206E-4 0.0 0.0 6.3349719976759795 0.0 9 3.183589656517206E-4 0.0 0.0 7.204887871319306 0.0 10 3.183589656517206E-4 0.0 0.0 8.34487826749051 0.0 11 3.714187932603407E-4 0.0 0.0 9.880111319518322 0.0 12 3.714187932603407E-4 0.0 0.0 11.219023009394242 0.0 13 3.714187932603407E-4 0.0 0.0 11.801832155847325 0.0 14 3.714187932603407E-4 0.0 0.0 12.057474405265657 0.0 15 3.714187932603407E-4 0.0 0.0 12.304520962611392 0.0 16 3.714187932603407E-4 0.0 0.0 12.775055513844636 0.0 17 3.714187932603407E-4 0.0 0.0 13.48282055431602 0.0 18 3.714187932603407E-4 0.0 0.0 14.24990648205384 0.0 19 3.714187932603407E-4 0.0 0.0 14.818071116086944 0.0 20 3.714187932603407E-4 0.0 0.0 15.375623784598323 0.0 21 3.714187932603407E-4 0.0 0.0 16.105780072320545 0.0 22 3.714187932603407E-4 0.0 0.0 16.86697635919381 0.0 23 3.714187932603407E-4 0.0 0.0 17.641225363658794 0.0 24 3.714187932603407E-4 0.0 0.0 18.249768526502056 0.0 25 3.714187932603407E-4 0.0 0.0 18.79071346897194 0.0 26 3.714187932603407E-4 0.0 0.0 19.275627233487118 0.0 27 4.244786208689608E-4 0.0 0.0 19.759214502312084 0.0 28 4.7753844847758087E-4 0.0 0.0 20.2677398901131 0.0 29 4.7753844847758087E-4 0.0 0.0 20.84009625052728 0.0 30 5.30598276086201E-4 0.0 0.0 21.47501014769203 0.0 31 5.30598276086201E-4 0.0 0.0 22.064982370872276 0.0 32 5.836581036948211E-4 0.0 0.0 22.625877808523 0.0 33 5.836581036948211E-4 0.0 0.0 23.15536182822942 0.0 34 5.836581036948211E-4 0.0 0.0 23.673597164482814 0.0 35 5.836581036948211E-4 0.0 0.0 24.228709080924197 0.0 36 5.836581036948211E-4 0.0 0.0 24.767001032013646 0.0 37 5.836581036948211E-4 0.0 0.0 25.31484375207265 0.0 38 6.367179313034412E-4 0.0 0.0 25.854833617645575 0.0 39 6.367179313034412E-4 0.0 0.0 26.40883127770718 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAACT 25 3.8917595E-5 45.0 34 CCGAAAC 20 7.034437E-4 45.0 10 CGTTTTT 27720 0.0 44.39123 1 TGTACGG 160 0.0 43.59375 2 CGTTATT 705 0.0 41.48936 1 TACGGGA 435 0.0 40.86207 4 TGTTACG 75 0.0 39.0 1 GTTTTTT 31735 0.0 38.98062 2 GTACGGG 250 0.0 38.699997 3 TCGCCCG 35 6.250415E-6 38.57143 1 GCGCTAG 35 6.250415E-6 38.57143 1 TAGCGGG 390 0.0 38.076923 3 GCGCGGC 30 1.1401992E-4 37.499996 3 GCGCGAC 30 1.1401992E-4 37.499996 9 TTTCGCG 535 0.0 37.009346 1 ACACGAC 425 0.0 36.52941 26 CGTTTAT 345 0.0 36.521736 1 CTAGCGG 185 0.0 36.48649 2 GTACGGC 105 0.0 36.42857 9 TTGGGAC 4120 0.0 36.152912 5 >>END_MODULE