##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933163.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1451024
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.166528603248466	33.0	31.0	34.0	30.0	34.0
2	32.34671928238265	34.0	31.0	34.0	30.0	34.0
3	32.42781304788894	34.0	31.0	34.0	30.0	34.0
4	35.90657287543142	37.0	35.0	37.0	35.0	37.0
5	35.09647531674183	37.0	35.0	37.0	35.0	37.0
6	34.793476193364135	37.0	35.0	37.0	32.0	37.0
7	35.60357168454829	37.0	35.0	37.0	33.0	37.0
8	35.895546868969774	37.0	35.0	37.0	35.0	37.0
9	37.74564238772067	39.0	38.0	39.0	35.0	39.0
10	37.22037333634729	39.0	37.0	39.0	34.0	39.0
11	37.145898344892984	39.0	37.0	39.0	34.0	39.0
12	37.00395513788883	39.0	37.0	39.0	33.0	39.0
13	36.9496183384975	39.0	37.0	39.0	33.0	39.0
14	38.08095868848482	40.0	37.0	41.0	33.0	41.0
15	38.18034987705234	40.0	37.0	41.0	33.0	41.0
16	38.18232020972775	40.0	37.0	41.0	33.0	41.0
17	38.12660645171961	40.0	37.0	41.0	33.0	41.0
18	38.070765886711726	40.0	37.0	41.0	33.0	41.0
19	38.07085203277134	40.0	37.0	41.0	34.0	41.0
20	37.99003117798189	40.0	37.0	41.0	33.0	41.0
21	37.85929936375966	40.0	36.0	41.0	33.0	41.0
22	37.905888531133876	40.0	36.0	41.0	33.0	41.0
23	37.81122779499167	40.0	36.0	41.0	33.0	41.0
24	37.729967250713976	40.0	36.0	41.0	33.0	41.0
25	37.62015032142818	39.0	36.0	41.0	33.0	41.0
26	37.582891116894004	39.0	36.0	41.0	33.0	41.0
27	37.554394689543386	39.0	36.0	41.0	33.0	41.0
28	37.451488052575286	39.0	36.0	41.0	33.0	41.0
29	37.37247764337461	39.0	35.0	41.0	32.0	41.0
30	37.30141541421782	39.0	35.0	41.0	32.0	41.0
31	37.23038350847401	39.0	35.0	41.0	32.0	41.0
32	37.18126578195812	39.0	35.0	41.0	32.0	41.0
33	37.0801633880625	39.0	35.0	41.0	32.0	41.0
34	36.98679070780359	39.0	35.0	41.0	31.0	41.0
35	36.839802098380176	39.0	35.0	41.0	31.0	41.0
36	36.72765233380013	39.0	35.0	41.0	31.0	41.0
37	36.70330194400644	39.0	35.0	41.0	31.0	41.0
38	36.59723615874031	39.0	35.0	41.0	31.0	41.0
39	36.533479804606955	39.0	35.0	41.0	30.0	41.0
40	36.41602619942882	39.0	35.0	40.0	30.0	41.0
41	36.299888906041524	39.0	35.0	40.0	30.0	41.0
42	36.21438859729405	38.0	35.0	40.0	30.0	41.0
43	36.06578388779234	38.0	35.0	40.0	30.0	41.0
44	36.00518943863093	38.0	35.0	40.0	30.0	41.0
45	36.01354905225551	38.0	35.0	40.0	30.0	41.0
46	35.7952928414692	38.0	35.0	40.0	29.0	41.0
47	35.70982768031404	38.0	35.0	40.0	29.0	41.0
48	35.62547552624905	38.0	34.0	40.0	28.0	41.0
49	35.666024131923386	38.0	34.0	40.0	29.0	41.0
50	35.470391943896175	37.0	34.0	40.0	28.0	41.0
51	34.37161893945241	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	23.0
10	33.0
11	29.0
12	21.0
13	23.0
14	28.0
15	51.0
16	91.0
17	149.0
18	312.0
19	609.0
20	1024.0
21	1766.0
22	2553.0
23	3791.0
24	5550.0
25	8190.0
26	11356.0
27	14253.0
28	16797.0
29	20501.0
30	25242.0
31	32161.0
32	41891.0
33	58175.0
34	94615.0
35	119569.0
36	131089.0
37	192479.0
38	301977.0
39	366520.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.268017620659617	4.477596511153503	53.491741004973036	16.76264486321384
2	20.758788276417206	4.719218979148518	52.75550232111943	21.766490423314846
3	21.98295824190365	4.864151109836915	50.798953015249914	22.353937633009515
4	18.97439325607295	5.0233490279967805	50.66277332421794	25.339484391712336
5	17.80900936166459	8.523084387301658	48.866455689223606	24.801450561810142
6	22.42202747852551	7.822682464245939	52.61966721432588	17.135622842902666
7	84.48075290277762	2.3471010817188414	8.827765770931425	4.3443802445721085
8	86.4052558744721	2.812634387852992	6.758744169634685	4.023365568040226
9	80.35063513766829	5.609004399651556	9.03706623736065	5.0032942253194985
10	38.74443151870679	28.598010784108325	16.438597845383672	16.21895985180121
11	30.64167098545579	25.3749076514241	24.077272326301976	19.906149036818135
12	27.954189593004664	21.422181852264334	30.17917002061992	20.44445853411108
13	24.995106903814136	22.23030080825679	31.612020200906393	21.16257208702268
14	21.39461511318903	25.03128824885047	30.679299584293574	22.894797053666927
15	19.51380542292891	23.75632656661778	34.63016462856576	22.09970338188755
16	22.427265158949815	21.266981111270386	32.65707527925107	23.648678450528728
17	21.960284599014212	21.13466076370894	30.2162472846762	26.688807352600648
18	22.60141803305803	22.20900550232112	30.34140028007807	24.848176184542776
19	25.667459669860733	24.014833662296418	28.208561677822008	22.10914499002084
20	28.301737255896526	23.361708696754842	27.05999349424958	21.276560553099053
21	27.04552095623505	24.034612797582948	26.95186296022671	21.968003285955298
22	24.922468546350714	23.532002227392518	26.461933090010913	25.083596136245852
23	23.887613161463904	25.151754898609536	26.725264365027734	24.23536757489883
24	23.440342819967142	23.49878430680678	28.41917156435731	24.64170130886877
25	23.826759584955177	24.064315958936586	26.554143832217797	25.554780623890437
26	22.093225198204856	25.15830199913992	26.854138870204764	25.894333932450465
27	22.30645392495231	24.59084067527484	28.178582849077614	24.924122550695234
28	20.91281743100045	24.742664490732064	29.517637199660378	24.826880878607106
29	21.587444453020762	24.298633241076647	28.18361377895886	25.93030852694373
30	23.28011114909195	23.65860247659584	27.945988488129764	25.115297886182447
31	23.945985731455856	25.522045121238516	25.57676509830299	24.955204049002635
32	23.36942738369593	26.20632050193519	25.291311515178243	25.13294059919064
33	23.48134834434165	25.38021424869609	25.41984143611684	25.718595970845413
34	22.695351696457124	24.39132640121735	26.842974340879266	26.070347561446262
35	21.858563331826353	24.309315352468325	26.07875541686423	27.753365898841093
36	21.989367508738656	26.406868528708	25.079392208536866	26.524371754016475
37	22.322166966225232	26.854621288138585	26.333609919615387	24.489601826020795
38	22.079303994971827	26.591841347903273	26.41548313466903	24.913371522455865
39	23.033388834368004	25.55112803096296	25.83789103419378	25.57759210047525
40	23.932960447242774	23.414636835779422	27.823178665549296	24.829224051428508
41	20.902066402761086	24.696421285933244	26.997072412310203	27.40443989899547
42	21.953599664788452	25.766699930531818	25.77379836584371	26.50590203883602
43	21.996328110355172	24.94913936640607	27.146897639184463	25.907634884054296
44	22.060420778705243	24.743353659208942	27.093004664292252	26.103220897793562
45	22.716164584458976	23.53703315727376	25.539412166855957	28.207390091411305
46	22.21913627893129	24.239089084673996	27.02050414052421	26.521270495870503
47	20.924050877173634	25.066918259105293	28.104910738898877	25.904120124822192
48	20.777051241054593	23.832272932770238	28.352597889490454	27.03807793668471
49	20.947689355930706	24.050946090485066	29.022952066954094	25.97841248663013
50	20.989177298239035	23.418013761316146	27.544478933498002	28.04833000694682
51	20.26465447849243	23.72255731125054	26.40073492926375	29.612053280993283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	935.0
2	1615.0
3	4134.5
4	6654.0
5	4647.0
6	2640.0
7	2581.0
8	2522.0
9	2592.5
10	2663.0
11	2748.5
12	2834.0
13	2922.0
14	3010.0
15	2941.0
16	2872.0
17	2957.5
18	3043.0
19	2920.5
20	2798.0
21	3421.0
22	4044.0
23	4401.0
24	4758.0
25	5735.5
26	8480.0
27	10247.0
28	11951.5
29	13656.0
30	16185.0
31	18714.0
32	22385.5
33	26057.0
34	30488.0
35	34919.0
36	36571.0
37	38223.0
38	43345.0
39	48467.0
40	53611.0
41	58755.0
42	63109.0
43	67463.0
44	73669.0
45	79875.0
46	88448.0
47	97021.0
48	107050.0
49	117079.0
50	127323.0
51	137567.0
52	134679.0
53	131791.0
54	119070.0
55	106349.0
56	99714.0
57	93079.0
58	86833.5
59	80588.0
60	75098.5
61	69609.0
62	61422.0
63	53235.0
64	46383.5
65	39532.0
66	33185.0
67	26838.0
68	22391.5
69	17945.0
70	15402.0
71	12859.0
72	11037.5
73	9216.0
74	7973.0
75	5664.0
76	4598.0
77	3392.5
78	2187.0
79	1749.5
80	1312.0
81	906.0
82	500.0
83	309.5
84	119.0
85	74.5
86	30.0
87	25.5
88	21.0
89	16.5
90	12.0
91	8.5
92	5.0
93	5.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1451024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.16574428254981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5311699409629	22.784539578008996
2	10.28358637037569	6.204240735125353
3	3.716756045920602	3.363561373255856
4	1.7655910760112359	2.1304147562602758
5	1.032969637775543	1.5580148972387569
6	0.6667873368043569	1.2068481775729585
7	0.49228966165619703	1.0395198832524664
8	0.3839124718421008	0.9264804361976334
9	0.30693769359233625	0.8333103578023837
>10	4.931944345370415	39.29905439295007
>50	0.8078949778952318	14.862942229413347
>100	0.07560587133480604	4.07291742973915
>500	0.002960470798094882	0.5601472156832223
>1k	0.0015940996605126288	1.1580085374996416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3416	0.23541995170307314	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	3272	0.22549592563596466	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3144	0.21667456913186828	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2858	0.1969643506930278	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2119	0.14603480025140866	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0675054306475978E-4	0.0	0.0	0.2344551158354376	0.0
2	2.0675054306475978E-4	0.0	0.0	0.8712467884748978	0.0
3	2.0675054306475978E-4	0.0	0.0	1.2568365512906747	0.0
4	2.0675054306475978E-4	0.0	0.0	1.738289649240812	0.0
5	2.0675054306475978E-4	0.0	0.0	2.5099515928061837	0.0
6	2.0675054306475978E-4	0.0	0.0	3.081754678075621	0.0
7	2.0675054306475978E-4	0.0	0.0	3.607796976480058	0.0
8	2.0675054306475978E-4	0.0	0.0	4.479250515498021	0.0
9	2.0675054306475978E-4	0.0	0.0	5.017491095943279	0.0
10	2.7566739075301306E-4	0.0	0.0	5.87757335509268	0.0
11	2.7566739075301306E-4	0.0	0.0	7.358734245608619	0.0
12	2.7566739075301306E-4	0.0	0.0	8.734107774923089	0.0
13	2.7566739075301306E-4	0.0	0.0	9.167870414272954	0.0
14	2.7566739075301306E-4	0.0	0.0	9.306186527583279	0.0
15	2.7566739075301306E-4	0.0	0.0	9.534508043974462	0.0
16	3.445842384412663E-4	0.0	0.0	10.068958197796865	0.0
17	4.1350108612951957E-4	0.0	0.0	10.844134900594339	0.0
18	4.1350108612951957E-4	0.0	0.0	11.674307247847038	0.0
19	4.1350108612951957E-4	0.0	0.0	12.240528068453726	0.0
20	4.1350108612951957E-4	0.0	0.0	12.8602283628665	0.0
21	4.1350108612951957E-4	0.0	0.0	13.711282514968739	0.0
22	4.1350108612951957E-4	0.0	0.0	14.642831545170859	0.0
23	4.1350108612951957E-4	0.0	0.0	15.52579419775276	0.0
24	4.1350108612951957E-4	0.0	0.0	16.173819316565403	0.0
25	4.8241793381777285E-4	0.0	0.0	16.73852396652295	0.0
26	4.8241793381777285E-4	0.0	0.0	17.230176757930952	0.0
27	4.8241793381777285E-4	0.0	0.0	17.726653728677128	0.0
28	4.8241793381777285E-4	0.0	0.0	18.23753432059015	0.0
29	4.8241793381777285E-4	0.0	0.0	18.82139785420503	0.0
30	4.8241793381777285E-4	0.0	0.0	19.46184211977197	0.0
31	5.513347815060261E-4	0.0	0.0	20.057076933255413	0.0
32	5.513347815060261E-4	0.0	0.0	20.643697140777824	0.0
33	5.513347815060261E-4	0.0	0.0	21.20688562008623	0.0
34	5.513347815060261E-4	0.0	0.0	21.768902512983935	0.0
35	5.513347815060261E-4	0.0	0.0	22.375715356879002	0.0
36	5.513347815060261E-4	0.0	0.0	22.96371390135518	0.0
37	6.202516291942793E-4	0.0	0.0	23.52318087088842	0.0
38	6.202516291942793E-4	0.0	0.0	24.081751921401715	0.0
39	6.891684768825326E-4	0.0	0.0	24.692079524528886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACA	20	7.0339E-4	45.0	21
CGATAGA	20	7.0339E-4	45.0	27
TACGTCC	25	3.8913142E-5	45.0	24
CGCTTAC	20	7.0339E-4	45.0	42
CGAACGA	60	3.6379788E-12	41.249996	22
CGATAGG	90	0.0	40.0	10
TACGTAG	40	3.459063E-7	39.375	1
TCACGTC	40	3.459063E-7	39.375	39
GCGATAG	105	0.0	38.57143	9
TACGCGA	35	6.2494237E-6	38.571426	37
CTACGTT	35	6.2494237E-6	38.571426	13
CCGATAA	65	9.094947E-12	38.07692	15
CGTTTTT	2215	0.0	37.78781	1
TTTGGGA	7630	0.0	37.77523	4
TCAAGCG	450	0.0	37.5	17
TACGAGT	30	1.14006965E-4	37.499996	31
TTGGGAT	3550	0.0	37.204227	5
TTTGGGC	4095	0.0	36.978024	4
TTGGGAC	2795	0.0	36.94991	5
ACATACG	250	0.0	36.9	17
>>END_MODULE