##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819119
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25400216574148	33.0	31.0	34.0	30.0	34.0
2	32.43429709236387	34.0	31.0	34.0	31.0	34.0
3	32.52288251157646	34.0	31.0	34.0	31.0	34.0
4	35.98121396280638	37.0	35.0	37.0	35.0	37.0
5	35.17682656610334	37.0	35.0	37.0	35.0	37.0
6	34.846806141720556	37.0	35.0	37.0	32.0	37.0
7	35.659390149660794	37.0	35.0	37.0	35.0	37.0
8	35.93905403244217	37.0	35.0	37.0	35.0	37.0
9	37.818194914292064	39.0	38.0	39.0	35.0	39.0
10	37.35475309448322	39.0	37.0	39.0	34.0	39.0
11	37.268236971673225	39.0	37.0	39.0	34.0	39.0
12	37.04329041323666	39.0	37.0	39.0	33.0	39.0
13	36.93785762508256	39.0	37.0	39.0	33.0	39.0
14	38.06148068839814	40.0	37.0	41.0	33.0	41.0
15	38.19992943638226	40.0	37.0	41.0	33.0	41.0
16	38.21393961072811	40.0	37.0	41.0	34.0	41.0
17	38.18214813720595	40.0	37.0	41.0	34.0	41.0
18	38.12654571557979	40.0	37.0	41.0	34.0	41.0
19	38.120791972839115	40.0	37.0	41.0	34.0	41.0
20	38.016080691572284	40.0	36.0	41.0	34.0	41.0
21	37.91330441608606	40.0	36.0	41.0	33.0	41.0
22	37.94347097308206	40.0	36.0	41.0	33.0	41.0
23	37.87100531180451	40.0	36.0	41.0	33.0	41.0
24	37.82713622806943	40.0	36.0	41.0	33.0	41.0
25	37.69380517360725	40.0	36.0	41.0	33.0	41.0
26	37.66166698611557	40.0	35.0	41.0	33.0	41.0
27	37.61491919977439	39.0	35.0	41.0	33.0	41.0
28	37.543649945856465	39.0	35.0	41.0	33.0	41.0
29	37.490454988835566	39.0	35.0	41.0	33.0	41.0
30	37.40735228947198	39.0	35.0	41.0	33.0	41.0
31	37.35705800988623	39.0	35.0	41.0	33.0	41.0
32	37.318210174590014	39.0	35.0	41.0	33.0	41.0
33	37.188744248393704	39.0	35.0	41.0	32.0	41.0
34	37.09408523059531	39.0	35.0	41.0	32.0	41.0
35	36.970745398409754	39.0	35.0	41.0	32.0	41.0
36	36.84629461653313	39.0	35.0	41.0	31.0	41.0
37	36.813528925589566	39.0	35.0	41.0	31.0	41.0
38	36.71368018566289	39.0	35.0	41.0	31.0	41.0
39	36.67118330791985	39.0	35.0	41.0	31.0	41.0
40	36.60610607249984	39.0	35.0	41.0	31.0	41.0
41	36.51635964981889	39.0	35.0	40.0	31.0	41.0
42	36.43591834641853	39.0	35.0	40.0	31.0	41.0
43	36.28561906145505	38.0	35.0	40.0	30.0	41.0
44	36.205883394232096	38.0	35.0	40.0	30.0	41.0
45	36.21324252031756	38.0	35.0	40.0	30.0	41.0
46	36.03013725722392	38.0	35.0	40.0	30.0	41.0
47	35.98498875010835	38.0	35.0	40.0	30.0	41.0
48	35.8872776727191	38.0	35.0	40.0	30.0	41.0
49	35.945995636775606	38.0	35.0	40.0	30.0	41.0
50	35.77667591644193	38.0	35.0	40.0	29.0	41.0
51	34.756887582878676	37.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	21.0
11	15.0
12	21.0
13	17.0
14	25.0
15	23.0
16	49.0
17	65.0
18	142.0
19	276.0
20	497.0
21	824.0
22	1233.0
23	1944.0
24	2715.0
25	4099.0
26	5700.0
27	7090.0
28	8325.0
29	10033.0
30	12703.0
31	16484.0
32	22066.0
33	31614.0
34	56216.0
35	75638.0
36	70391.0
37	104325.0
38	168352.0
39	218084.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.53719300858606	4.736918567387645	52.394096584256985	17.331791839769313
2	20.37603815806983	4.546836296069314	52.18374863725539	22.893376908605465
3	21.25564173215369	4.620207808633422	51.24774300193257	22.87640745728032
4	18.837678041896233	5.064099355527097	51.514859257324034	24.583363345252643
5	17.650793108205278	8.405372113209436	49.444586195656555	24.499248582928733
6	21.5554760663591	8.491440193671492	52.812594995354765	17.14048874461464
7	83.53609182548567	2.3126065931812105	9.521449264392597	4.629852316940518
8	85.61112610011487	3.2000234398176577	6.728692656378376	4.460157803689086
9	80.04954103127872	5.589908181839269	9.132861037285181	5.227689749596823
10	40.136903185007306	27.077506442897796	16.298120297539185	16.487470074555713
11	32.055049388428294	24.28035486907275	24.05486870650052	19.609727035998432
12	28.832318625254693	21.49956233465467	30.267397044873825	19.400721995216813
13	24.54710487731331	23.173067649511243	31.94541940792486	20.334408065250592
14	19.21149430058392	26.57049830366528	31.4533053194957	22.764702076255098
15	19.008959626134907	23.35265083583704	36.778538893616194	20.85985064441186
16	20.60909342842737	21.18153772528778	34.452503238235224	23.756865608049623
17	20.895498700433027	21.4324170236559	30.50533561057673	27.16674866533434
18	22.54446545617914	21.920014063890594	31.172882084288116	24.362638395642147
19	25.366155589114648	23.82596423718654	28.384398359701095	22.42348181399772
20	27.61015188269348	23.749296500264307	28.05819423063071	20.5823573864115
21	26.060560187225544	24.644648701836974	27.313980019997093	21.98081109094039
22	23.78958368686357	23.927780945137396	26.81686055383894	25.465774814160092
23	23.123380119372154	25.6003096009249	26.642038580474875	24.634271699228073
24	24.135320997315407	22.67851191340941	29.4047629221151	23.781404167160083
25	22.155266817153553	24.681639664078116	27.760069049796183	25.403024468972152
26	20.990112547749472	25.501056623030355	28.664211182990506	24.844619646229667
27	20.627039538821588	25.796862238575834	29.249474130132498	24.32662409247008
28	19.713741226854705	24.988066446999767	31.3348854073706	23.963306918774926
29	20.81211643241092	24.904806261361294	29.66760629407937	24.61547101214842
30	21.443770685333877	26.48162232837964	28.104097206877142	23.97050977940934
31	23.41186079189959	26.824429661624254	25.08976107256699	24.673948473909164
32	24.056577859871396	27.472320871570556	25.36542309481284	23.105678173745208
33	23.589368577703606	26.95652280071638	25.969852976185386	23.484255645394626
34	21.310212557638145	25.13896027317154	27.586345817884826	25.96448135130549
35	20.88219172061691	25.45417698771485	28.65700832235609	25.006622969312154
36	22.348401148062734	27.226569033315062	27.24341640225657	23.181613416365632
37	21.428876634530514	29.190752503604482	26.46514120658903	22.915229655275972
38	21.1383205614813	28.599141272513517	25.903928489022967	24.35860967698222
39	21.31656084158712	27.03465552624222	27.420680023293322	24.228103608877344
40	23.061362268486018	24.985991046477984	28.37426552185946	23.578381163176534
41	20.56978290089718	24.574451331247353	27.822819394984123	27.03294637287134
42	22.31092185628706	24.598379478439643	27.816593193418782	25.27410547185452
43	21.915497015696133	24.42819663565367	28.206524326746173	25.44978202190402
44	21.3701550080025	25.336245405124284	27.168946148239755	26.12465343863346
45	21.010134058665468	25.955447254916564	26.47698319780154	26.557435488616427
46	21.19582136417297	26.811488928959037	27.801943307382686	24.1907463994853
47	20.234544675437878	25.29901027811588	29.214924815564036	25.251520230882203
48	20.11380519802373	24.66235064746392	29.83986453738712	25.38397961712523
49	20.951778679288356	23.772492153154793	30.311835032516644	24.963894135040206
50	20.713107619283644	24.373259563018316	28.66994905502131	26.243683762676728
51	19.708369601974805	24.684691723668966	27.29762098059012	28.309317693766108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	615.0
2	1121.0
3	2494.0
4	3867.0
5	2660.0
6	1453.0
7	1478.0
8	1503.0
9	1522.5
10	1542.0
11	1617.0
12	1692.0
13	1758.5
14	1825.0
15	1878.5
16	1932.0
17	1813.0
18	1694.0
19	1583.0
20	1472.0
21	1961.5
22	2451.0
23	2746.5
24	3042.0
25	3674.0
26	4944.0
27	5582.0
28	7268.5
29	8955.0
30	9730.0
31	10505.0
32	12322.0
33	14139.0
34	16956.0
35	19773.0
36	21105.5
37	22438.0
38	26071.5
39	29705.0
40	35293.0
41	40881.0
42	47670.0
43	54459.0
44	61721.0
45	68983.0
46	74700.5
47	80418.0
48	78335.0
49	76252.0
50	68566.0
51	60880.0
52	56122.0
53	51364.0
54	48035.5
55	44707.0
56	42022.0
57	39337.0
58	39579.0
59	39821.0
60	37018.5
61	34216.0
62	31060.5
63	27905.0
64	23650.5
65	19396.0
66	16852.0
67	14308.0
68	11901.5
69	9495.0
70	8031.0
71	6567.0
72	5731.0
73	4895.0
74	3979.5
75	2225.5
76	1387.0
77	1206.0
78	1025.0
79	717.5
80	410.0
81	278.5
82	147.0
83	103.0
84	59.0
85	39.5
86	20.0
87	13.5
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	1.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	819119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.171554718032766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95766948663203	20.835025327608363
2	11.189110345282044	6.304292686764391
3	4.30154552056627	3.6354367501422424
4	1.9402721475036813	2.1864193188509957
5	1.0814430813099856	1.5232966469781108
6	0.7109751847705611	1.201756579255639
7	0.4774006997119805	0.9414383954054232
8	0.38307476615570935	0.8633449388682537
9	0.31115992572636514	0.7889272986293376
>10	4.181454804662001	31.20119962632823
>50	1.370918200026813	24.453686629552102
>100	0.09024853351602027	4.176141766991518
>500	0.002148774607524292	0.38790393174003657
>1k	0.0025785295290291508	1.5011301028853663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2650	0.3235183166304285	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2370	0.28933524921287385	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2301	0.28091156474211926	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1998	0.2439206025009797	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	1986	0.24245561389737025	No Hit
TTTCCTGGGGTTTTATCCATTCACTCATAGCAGGTGATTAGACGATCTTGA	1094	0.13355812769573164	No Hit
TCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	879	0.10731041521439498	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4416476726824796E-4	0.0	0.0	0.3039851352489687	0.0
2	2.4416476726824796E-4	0.0	0.0	1.2299800151137992	0.0
3	2.4416476726824796E-4	0.0	0.0	1.749806804627899	0.0
4	2.4416476726824796E-4	0.0	0.0	2.324936913928257	0.0
5	2.4416476726824796E-4	0.0	0.0	3.231642777178896	0.0
6	2.4416476726824796E-4	0.0	0.0	3.8446184254058324	0.0
7	2.4416476726824796E-4	0.0	0.0	4.407418213959144	0.0
8	2.4416476726824796E-4	0.0	0.0	5.329872704698585	0.0
9	3.662471509023719E-4	0.0	0.0	5.870941828965022	0.0
10	3.662471509023719E-4	0.0	0.0	6.856268747276037	0.0
11	3.662471509023719E-4	0.0	0.0	8.272180232664606	0.0
12	4.883295345364959E-4	0.0	0.0	9.635596293090503	0.0
13	4.883295345364959E-4	0.0	0.0	10.050798479830158	0.0
14	4.883295345364959E-4	0.0	0.0	10.193879033449353	0.0
15	4.883295345364959E-4	0.0	0.0	10.425103068052383	0.0
16	4.883295345364959E-4	0.0	0.0	10.999134435900034	0.0
17	4.883295345364959E-4	0.0	0.0	11.851635720817121	0.0
18	4.883295345364959E-4	0.0	0.0	12.793501310554388	0.0
19	4.883295345364959E-4	0.0	0.0	13.35569068718953	0.0
20	4.883295345364959E-4	0.0	0.0	13.94986564833681	0.0
21	4.883295345364959E-4	0.0	0.0	14.753411897416616	0.0
22	4.883295345364959E-4	0.0	0.0	15.581618787990513	0.0
23	4.883295345364959E-4	0.0	0.0	16.36636434999066	0.0
24	4.883295345364959E-4	0.0	0.0	16.977020432928548	0.0
25	4.883295345364959E-4	0.0	0.0	17.524559923527594	0.0
26	4.883295345364959E-4	0.0	0.0	18.002390373071556	0.0
27	4.883295345364959E-4	0.0	0.0	18.455071851586887	0.0
28	6.104119181706199E-4	0.0	0.0	18.927774841018216	0.0
29	6.104119181706199E-4	0.0	0.0	19.450775772506802	0.0
30	6.104119181706199E-4	0.0	0.0	20.051176935219424	0.0
31	6.104119181706199E-4	0.0	0.0	20.57527660816072	0.0
32	6.104119181706199E-4	0.0	0.0	21.092539667618503	0.0
33	6.104119181706199E-4	0.0	0.0	21.633364627117672	0.0
34	6.104119181706199E-4	0.0	0.0	22.148796450820942	0.0
35	6.104119181706199E-4	0.0	0.0	22.746023471559077	0.0
36	6.104119181706199E-4	0.0	0.0	23.24985746881711	0.0
37	6.104119181706199E-4	0.0	0.0	23.766632198740353	0.0
38	6.104119181706199E-4	0.0	0.0	24.287679812090794	0.0
39	6.104119181706199E-4	0.0	0.0	24.77417811087278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTT	20	7.032097E-4	45.000004	42
CGAGTTA	20	7.032097E-4	45.000004	36
ACGCATT	20	7.032097E-4	45.000004	12
CGTGAAT	20	7.032097E-4	45.000004	40
CACGCGG	40	6.8102963E-9	45.000004	37
CGCGAAT	20	7.032097E-4	45.000004	4
CCGTAAA	20	7.032097E-4	45.000004	30
GTCACGA	20	7.032097E-4	45.000004	40
CGTACAT	20	7.032097E-4	45.000004	26
CGGTCAG	20	7.032097E-4	45.000004	44
AGAATCG	20	7.032097E-4	45.000004	17
ATCGCGA	20	7.032097E-4	45.000004	20
TACACGA	20	7.032097E-4	45.000004	20
ACGAATT	20	7.032097E-4	45.000004	20
TACGTAT	25	3.8898215E-5	45.0	20
CAACGAC	30	2.1647356E-6	44.999996	12
CTATTAG	30	2.1647356E-6	44.999996	23
GCGATTC	295	0.0	42.711864	9
TAATGCG	80	0.0	42.187504	24
CGACAAT	70	0.0	41.785713	20
>>END_MODULE