FastQCFastQC Report
Sat 14 Jan 2017
SRR2933161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317604
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142690.6156789511926973No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27270.11766462260161788No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC24550.10592836394828452TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT23210.10014653064112766Illumina PCR Primer Index 3 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70600.041.3349881
TTAAACG356.251037E-638.5714345
TTGGGAC50900.038.546175
TATGGGC4550.038.0769234
CGAATAT3550.038.0281714
TTTGGGA117050.037.6377644
TCGACGT301.1402799E-437.50000426
ACGGTAC301.1402799E-437.50000413
TCGTTTG9150.037.377051
TTTGGGC61800.037.2087364
TGGGCGA40000.036.33756
ACACGAC11050.036.24434726
TACGAAT3750.036.012
TCGGTAT250.0021075536.025
TCAAGCG11350.035.6828217
CGTTTGG16150.035.6656342
TTTGTCG5700.035.5263181
GGGCGAT64150.035.4247867
TCGTTCA3950.035.31645616
CACGACC11350.035.28634327
Produced by FastQC (version 0.11.5)