##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2317604
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.373136653198735	33.0	31.0	34.0	31.0	34.0
2	32.5776176603078	34.0	31.0	34.0	31.0	34.0
3	32.621480632584344	34.0	31.0	34.0	31.0	34.0
4	36.06592584410451	37.0	35.0	37.0	35.0	37.0
5	35.27258021646494	37.0	35.0	37.0	35.0	37.0
6	34.97276195588202	37.0	35.0	37.0	33.0	37.0
7	35.7239282465857	37.0	35.0	37.0	35.0	37.0
8	36.003846213589554	37.0	35.0	37.0	35.0	37.0
9	37.87648148691493	39.0	38.0	39.0	35.0	39.0
10	37.44969546134715	39.0	37.0	39.0	35.0	39.0
11	37.37707477204906	39.0	37.0	39.0	35.0	39.0
12	37.24059287091324	39.0	37.0	39.0	34.0	39.0
13	37.2190162771552	39.0	37.0	39.0	34.0	39.0
14	38.45097782019707	40.0	38.0	41.0	34.0	41.0
15	38.52602860540455	40.0	38.0	41.0	34.0	41.0
16	38.517063311937676	40.0	38.0	41.0	34.0	41.0
17	38.45338634210158	40.0	38.0	41.0	34.0	41.0
18	38.37842918807527	40.0	38.0	41.0	34.0	41.0
19	38.33872395801871	40.0	37.0	41.0	34.0	41.0
20	38.238181760128136	40.0	37.0	41.0	34.0	41.0
21	38.128937903110284	40.0	37.0	41.0	34.0	41.0
22	38.144393951684584	40.0	37.0	41.0	34.0	41.0
23	38.06762069792769	40.0	37.0	41.0	34.0	41.0
24	38.00170046306444	40.0	36.0	41.0	34.0	41.0
25	37.88892407848795	40.0	36.0	41.0	33.0	41.0
26	37.83234193589586	40.0	36.0	41.0	33.0	41.0
27	37.79223801822917	40.0	36.0	41.0	33.0	41.0
28	37.69332854102772	40.0	36.0	41.0	33.0	41.0
29	37.616709325665646	40.0	36.0	41.0	33.0	41.0
30	37.520958282778246	40.0	36.0	41.0	33.0	41.0
31	37.43414880195236	40.0	35.0	41.0	33.0	41.0
32	37.35389177788785	40.0	35.0	41.0	33.0	41.0
33	37.194906032264356	39.0	35.0	41.0	32.0	41.0
34	37.03298579049743	39.0	35.0	41.0	32.0	41.0
35	36.86659196307911	39.0	35.0	41.0	31.0	41.0
36	36.7362866995397	39.0	35.0	41.0	31.0	41.0
37	36.7161465030264	39.0	35.0	41.0	31.0	41.0
38	36.59991784618943	39.0	35.0	41.0	31.0	41.0
39	36.53583873690242	39.0	35.0	41.0	31.0	41.0
40	36.386829242614354	39.0	35.0	41.0	30.0	41.0
41	36.23641959540974	39.0	35.0	41.0	30.0	41.0
42	36.13387274098595	39.0	35.0	40.0	30.0	41.0
43	36.00354417752127	38.0	35.0	40.0	30.0	41.0
44	35.90035010295115	38.0	35.0	40.0	29.0	41.0
45	35.859736175809154	38.0	35.0	40.0	29.0	41.0
46	35.638366174721824	38.0	35.0	40.0	29.0	41.0
47	35.54236573633805	38.0	35.0	40.0	28.0	41.0
48	35.43817666866298	38.0	34.0	40.0	28.0	41.0
49	35.45945813003429	37.0	34.0	40.0	28.0	41.0
50	35.277152179578565	37.0	34.0	40.0	28.0	41.0
51	34.15318492719205	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	34.0
10	58.0
11	48.0
12	43.0
13	50.0
14	59.0
15	79.0
16	163.0
17	290.0
18	494.0
19	866.0
20	1617.0
21	2488.0
22	3954.0
23	5901.0
24	8976.0
25	13976.0
26	19976.0
27	24778.0
28	27564.0
29	30770.0
30	36616.0
31	45537.0
32	58949.0
33	82551.0
34	141353.0
35	181206.0
36	206944.0
37	301271.0
38	476232.0
39	644451.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.364951044268132	4.21538796101491	54.6193396283403	18.800321366376654
2	22.06485663642279	4.443770376647606	51.53020101794784	21.96117196898176
3	20.55877535592793	4.5305841722744695	52.14311849651623	22.76752197528137
4	18.573319687056113	4.764575829175303	51.170217172562694	25.491887311205886
5	17.71286207652386	7.747872371638986	49.666854216682395	24.872411335154755
6	22.198615466662986	7.341418119747808	52.7845568095326	17.675409604056604
7	83.59706835162521	2.1432479405454945	10.082524883457225	4.177158824372067
8	86.30011857073082	2.7295430970951036	7.609237816296485	3.3611005158776046
9	80.37632831148031	5.240196340703589	9.421540522021882	4.961934825794225
10	39.150907575237184	28.386644137652507	16.535611778371113	15.926836508739198
11	30.773980369381484	24.54491794111505	24.887038510461668	19.794063179041803
12	26.975963106725736	21.08759736348401	30.86049212894006	21.07594740085019
13	24.646488356078088	21.093336048781413	32.884435822513254	21.37573977262725
14	21.55057550815411	22.871034050683377	32.37766244794192	23.200727993220585
15	20.472436188408373	22.241461440349603	34.3512955621409	22.934806809101126
16	22.943004930954555	20.995648954696318	33.00270451725144	23.058641597097694
17	22.750176475359897	21.28184970340058	31.170553727038786	24.797420094200735
18	23.51299013981681	21.338761928267296	30.6042360990057	24.544011832910194
19	24.759924473723725	23.512774399768034	29.103203135652166	22.62409799085607
20	27.800348981102896	22.919144081560095	27.86084249077927	21.419664446557736
21	27.345568958286233	23.51104847937784	27.039304385045938	22.104078177289992
22	25.599757335593136	22.9724318736074	26.767773959658335	24.660036831141127
23	24.86990875058897	24.477952229975443	26.70115343259677	23.950985586838822
24	24.16055547021838	22.749960735311124	28.870505919043975	24.218977875426518
25	24.89402848804196	23.802858469350245	26.78317779914084	24.519935243466957
26	23.72536464383044	24.64096541082946	26.92392660696133	24.70974333837877
27	23.19119228306475	23.884278763757745	28.50694078884917	24.417588164328333
28	22.457848709270436	24.941189262704068	29.22073831422452	23.380223713800977
29	23.450080341594166	23.465613625105927	28.257415848436573	24.82689018486333
30	24.316794413540883	23.244480075112055	28.694246299195203	23.744479212151862
31	24.235115231074854	24.98554541673211	26.99632896733005	23.783010384862987
32	24.87405095952544	24.333708433364805	27.483426849453142	23.308813757656615
33	25.16193448061015	24.154428452833184	26.415945088116864	24.267691978439803
34	24.07939406386941	24.201804967544067	27.836852197355544	23.88194877123098
35	24.31843403791157	24.047766572719066	27.424831852205983	24.20896753716338
36	23.239388609960976	26.043275727863772	27.030415895036423	23.68691976713882
37	24.492579405282353	25.26372063562196	27.639363756707358	22.60433620238833
38	23.982095301872107	24.343848215657204	27.995680021263343	23.67837646120735
39	24.6985248558425	23.453489034364804	26.85437201523643	24.993614094556275
40	24.97790821900549	23.269807956838182	28.794090793768046	22.95819303038828
41	21.905424740378425	24.34963004896436	29.085037823545353	24.65990738711186
42	22.398951675954994	24.786762535791272	27.906924565197507	24.907361223056228
43	22.57469351968671	23.676132764700096	28.648509408855006	25.100664306758187
44	23.286376792584065	24.157233073467253	27.943470929459906	24.612919204488772
45	23.650977475012986	23.210910923522743	27.189286866953978	25.948824734510296
46	23.041770725283524	24.388765293812057	27.78347810928873	24.785985871615686
47	22.48956249644029	24.59604833267461	29.619425924359817	23.294963246525292
48	22.70504365715627	22.81796199868485	29.474146575515057	25.00284776864382
49	22.526756080849015	23.829739679427547	29.013498423371725	24.630005816351716
50	22.165434647161465	23.1264702684324	29.220134242087948	25.487960842318184
51	21.682090641887054	22.95094416474946	28.435013056587756	26.931952136775738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	332.0
1	1696.5
2	3061.0
3	14056.0
4	25051.0
5	16596.0
6	8141.0
7	7800.0
8	7459.0
9	7426.0
10	7393.0
11	7294.0
12	7195.0
13	6804.0
14	6413.0
15	6271.5
16	6130.0
17	5854.0
18	5578.0
19	5722.5
20	5867.0
21	6022.5
22	6178.0
23	6951.0
24	7724.0
25	9168.5
26	13377.0
27	16141.0
28	18361.5
29	20582.0
30	24918.0
31	29254.0
32	34152.5
33	39051.0
34	46922.5
35	54794.0
36	57835.5
37	60877.0
38	68233.5
39	75590.0
40	83411.5
41	91233.0
42	99608.0
43	107983.0
44	120221.5
45	132460.0
46	146665.5
47	160871.0
48	176161.5
49	191452.0
50	191151.0
51	190850.0
52	177983.0
53	165116.0
54	154197.0
55	143278.0
56	138457.0
57	133636.0
58	133914.5
59	134193.0
60	128804.0
61	123415.0
62	111792.0
63	100169.0
64	87109.0
65	74049.0
66	63584.5
67	53120.0
68	45673.5
69	38227.0
70	32368.5
71	26510.0
72	23277.5
73	20045.0
74	16236.5
75	10105.0
76	7782.0
77	5711.0
78	3640.0
79	2905.0
80	2170.0
81	1556.5
82	943.0
83	646.5
84	350.0
85	251.5
86	153.0
87	113.5
88	74.0
89	46.0
90	18.0
91	15.0
92	12.0
93	7.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2317604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.721183065567526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09195898754022	19.057328408050434
2	9.58690598617397	4.931731278055317
3	3.4678515782564956	2.6759173586565774
4	1.713697322298394	1.7631329018323945
5	1.008911632583593	1.29752003993316
6	0.7025295918540726	1.0841935344634221
7	0.5326186774395686	0.9589707754594522
8	0.4542111641201851	0.9346278802207854
9	0.3775363855146538	0.8739614237161577
>10	7.101931472975405	46.90693850070341
>50	0.873957988040932	13.693402230730722
>100	0.08082380353913984	3.556182545815128
>500	0.005342139013849098	0.9004977579252026
>1k	0.001550943584665867	0.7331282199292602
>5k	0.0	0.0
>10k+	1.7232706496287414E-4	0.6324671445085015
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14269	0.6156789511926973	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2727	0.11766462260161788	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2455	0.10592836394828452	TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	2321	0.10014653064112766	Illumina PCR Primer Index 3 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7259203901960819E-4	0.0	0.0	0.10825835647504924	0.0
2	1.7259203901960819E-4	0.0	0.0	0.40869794839843215	0.0
3	1.7259203901960819E-4	0.0	0.0	0.6649971263425504	0.0
4	1.7259203901960819E-4	0.0	0.0	0.9775613090070607	0.0
5	1.7259203901960819E-4	0.0	0.0	1.548711514132699	0.0
6	1.7259203901960819E-4	0.0	0.0	2.2732960419467694	0.0
7	1.7259203901960819E-4	0.0	0.0	2.8368953453652996	0.0
8	1.7259203901960819E-4	0.0	0.0	3.6589080792059385	0.0
9	1.7259203901960819E-4	0.0	0.0	4.231525316663244	0.0
10	1.7259203901960819E-4	0.0	0.0	5.0241974038705495	0.0
11	1.7259203901960819E-4	0.0	0.0	6.180607213311679	0.0
12	1.7259203901960819E-4	0.0	0.0	7.168394600630651	0.0
13	1.7259203901960819E-4	0.0	0.0	7.496405770787416	0.0
14	1.7259203901960819E-4	0.0	0.0	7.624167027671682	0.0
15	1.7259203901960819E-4	0.0	0.0	7.7841166998331035	0.0
16	1.7259203901960819E-4	0.0	0.0	8.130854106223497	0.0
17	1.7259203901960819E-4	0.0	0.0	8.595256135215507	0.0
18	1.7259203901960819E-4	0.0	0.0	9.099828961289331	0.0
19	1.7259203901960819E-4	0.0	0.0	9.451657832830803	0.0
20	1.7259203901960819E-4	0.0	0.0	9.83796196416644	0.0
21	1.7259203901960819E-4	0.0	0.0	10.370322108522423	0.0
22	1.7259203901960819E-4	0.0	0.0	10.946089150691835	0.0
23	2.1574004877451022E-4	0.0	0.0	11.483885944276935	0.0
24	2.1574004877451022E-4	0.0	0.0	11.930036365142621	0.0
25	2.1574004877451022E-4	0.0	0.0	12.33174433596076	0.0
26	2.1574004877451022E-4	0.0	0.0	12.678999518468212	0.0
27	2.1574004877451022E-4	0.0	0.0	13.033330974575467	0.0
28	2.1574004877451022E-4	0.0	0.0	13.387446690633947	0.0
29	2.1574004877451022E-4	0.0	0.0	13.778195066974341	0.0
30	2.1574004877451022E-4	0.0	0.0	14.234528418142185	0.0
31	2.1574004877451022E-4	0.0	0.0	14.639860821779735	0.0
32	2.1574004877451022E-4	0.0	0.0	15.058223924363265	0.0
33	2.1574004877451022E-4	0.0	0.0	15.454063765854736	0.0
34	2.5888805852941225E-4	0.0	0.0	15.838641976800178	0.0
35	2.5888805852941225E-4	0.0	0.0	16.273703359158855	0.0
36	2.5888805852941225E-4	0.0	0.0	16.68779480877665	0.0
37	2.5888805852941225E-4	0.0	0.0	17.09981515392621	0.0
38	2.5888805852941225E-4	0.0	0.0	17.523312869670573	0.0
39	2.5888805852941225E-4	0.0	0.0	17.94314300458577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7060	0.0	41.334988	1
TTAAACG	35	6.251037E-6	38.57143	45
TTGGGAC	5090	0.0	38.54617	5
TATGGGC	455	0.0	38.076923	4
CGAATAT	355	0.0	38.02817	14
TTTGGGA	11705	0.0	37.637764	4
TCGACGT	30	1.1402799E-4	37.500004	26
ACGGTAC	30	1.1402799E-4	37.500004	13
TCGTTTG	915	0.0	37.37705	1
TTTGGGC	6180	0.0	37.208736	4
TGGGCGA	4000	0.0	36.3375	6
ACACGAC	1105	0.0	36.244347	26
TACGAAT	375	0.0	36.0	12
TCGGTAT	25	0.00210755	36.0	25
TCAAGCG	1135	0.0	35.68282	17
CGTTTGG	1615	0.0	35.665634	2
TTTGTCG	570	0.0	35.526318	1
GGGCGAT	6415	0.0	35.424786	7
TCGTTCA	395	0.0	35.316456	16
CACGACC	1135	0.0	35.286343	27
>>END_MODULE