SRR2933159.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404471
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2810	0.6947346039641903	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2803	0.6930039483671264	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2319	0.5733414756558567	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2245	0.5550459736297534	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1035	0.25588979185157895	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	857	0.2118816923833847	RNA PCR Primer, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	573	0.1416665224453669	Illumina PCR Primer Index 7 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	518	0.1280685141827226	Illumina PCR Primer Index 7 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	486	0.12015694573900229	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	465	0.11496497894781085	RNA PCR Primer, Index 16 (95% over 24bp)
TTCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	439	0.10853682958728808	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	20	7.027858E-4	45.000004	14
TTGGCGA	20	7.027858E-4	45.000004	5
TCGCACT	20	7.027858E-4	45.000004	33
CCCTCGC	20	7.027858E-4	45.000004	44
CCCTCAT	20	7.027858E-4	45.000004	27
CTCAAAT	20	7.027858E-4	45.000004	11
TTTCGAG	20	7.027858E-4	45.000004	1
CGTGAGA	20	7.027858E-4	45.000004	42
GACCGTG	20	7.027858E-4	45.000004	39
CGTGACA	20	7.027858E-4	45.000004	33
GACCGGT	20	7.027858E-4	45.000004	10
CGACCGT	20	7.027858E-4	45.000004	38
GGATCGA	20	7.027858E-4	45.000004	8
ATAGATT	20	7.027858E-4	45.000004	11
ATTCGGA	20	7.027858E-4	45.000004	26
TGAGTAG	20	7.027858E-4	45.000004	28
TAACGGG	20	7.027858E-4	45.000004	3
TAACGCA	20	7.027858E-4	45.000004	10
GCGTTAG	20	7.027858E-4	45.000004	1
ACGGGAT	20	7.027858E-4	45.000004	5