##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404471
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0099117118409	33.0	31.0	34.0	30.0	34.0
2	32.19989319382601	34.0	31.0	34.0	30.0	34.0
3	32.27910777286876	34.0	31.0	34.0	30.0	34.0
4	35.8143674082938	37.0	35.0	37.0	35.0	37.0
5	35.03634871226862	37.0	35.0	37.0	33.0	37.0
6	34.76908604077919	37.0	35.0	37.0	32.0	37.0
7	35.581779657874115	37.0	35.0	37.0	33.0	37.0
8	35.84746496040507	37.0	35.0	37.0	35.0	37.0
9	37.68271396466001	39.0	37.0	39.0	35.0	39.0
10	37.17803006890482	39.0	37.0	39.0	34.0	39.0
11	37.07383718486616	39.0	37.0	39.0	33.0	39.0
12	36.69754815549199	39.0	35.0	39.0	33.0	39.0
13	36.46124196790375	39.0	35.0	39.0	32.0	39.0
14	37.39320742401804	39.0	36.0	41.0	33.0	41.0
15	37.565956520986674	39.0	36.0	41.0	33.0	41.0
16	37.6660353894346	39.0	36.0	41.0	33.0	41.0
17	37.646229272308766	39.0	35.0	41.0	33.0	41.0
18	37.64833325504177	39.0	36.0	41.0	33.0	41.0
19	37.658709771528734	39.0	36.0	41.0	33.0	41.0
20	37.55718209710956	39.0	35.0	41.0	33.0	41.0
21	37.409796994098464	39.0	35.0	41.0	33.0	41.0
22	37.48133982411594	39.0	35.0	41.0	33.0	41.0
23	37.44162869525874	39.0	35.0	41.0	33.0	41.0
24	37.42447295356157	39.0	35.0	41.0	33.0	41.0
25	37.28093237834109	39.0	35.0	41.0	33.0	41.0
26	37.225247298323	39.0	35.0	41.0	33.0	41.0
27	37.17045474211995	39.0	35.0	41.0	32.0	41.0
28	37.09471136373189	39.0	35.0	40.0	32.0	41.0
29	37.05555404466575	39.0	35.0	40.0	32.0	41.0
30	36.97136506696401	39.0	35.0	40.0	32.0	41.0
31	36.84379102580902	39.0	35.0	40.0	32.0	41.0
32	36.83878201403809	39.0	35.0	40.0	32.0	41.0
33	36.73659669049203	38.0	35.0	40.0	31.0	41.0
34	36.61571781413253	38.0	35.0	40.0	31.0	41.0
35	36.53146702730233	38.0	35.0	40.0	31.0	41.0
36	36.36619436251301	38.0	35.0	40.0	30.0	41.0
37	36.32198600146859	38.0	35.0	40.0	30.0	41.0
38	36.233050082700615	38.0	35.0	40.0	30.0	41.0
39	36.248710043488906	38.0	35.0	40.0	30.0	41.0
40	36.105028543455525	38.0	35.0	40.0	30.0	41.0
41	36.03641546612736	38.0	35.0	40.0	30.0	41.0
42	35.992041456618644	38.0	35.0	40.0	30.0	41.0
43	35.856016871419705	38.0	35.0	40.0	30.0	41.0
44	35.757453562801786	38.0	35.0	40.0	30.0	41.0
45	35.76756059148864	38.0	35.0	40.0	30.0	41.0
46	35.526035241092686	37.0	34.0	40.0	29.0	41.0
47	35.45119674834537	37.0	34.0	40.0	29.0	41.0
48	35.432330129972236	37.0	34.0	40.0	29.0	41.0
49	35.544479085027106	37.0	34.0	40.0	29.0	41.0
50	35.32432238652462	37.0	34.0	40.0	28.0	41.0
51	34.207354791814495	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	5.0
11	7.0
12	7.0
13	5.0
14	9.0
15	7.0
16	17.0
17	28.0
18	79.0
19	147.0
20	216.0
21	414.0
22	648.0
23	1019.0
24	1450.0
25	2266.0
26	3188.0
27	3984.0
28	4833.0
29	5970.0
30	7679.0
31	10007.0
32	13478.0
33	19298.0
34	33743.0
35	43812.0
36	36203.0
37	51521.0
38	82471.0
39	81918.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.607202988594977	4.538273448529067	53.4201463145689	17.434377248307047
2	19.53984340039212	4.4861065441032855	52.691787544718906	23.282262510785692
3	20.15645126597457	4.553107639361042	52.29843425115769	22.992006843506704
4	19.257499301556848	4.919759389424706	51.384153622880255	24.43858768613819
5	16.575724835649527	8.403569106314174	50.79770861198949	24.22299744604681
6	20.282294651532496	7.118680943751221	54.510212104205245	18.088812300511037
7	81.55343646392448	1.7402978211046034	10.94936348959505	5.756902225375861
8	82.54856343223618	2.4778043419676563	8.690363462399036	6.283268763397129
9	76.12659498456009	5.725008715087114	11.727664035246036	6.4207322651067695
10	40.61576726143531	25.765753292572274	18.727671452341454	14.890807993650967
11	32.85278796254861	24.195059719979923	24.11816916416752	18.833983153303947
12	30.15543759626772	20.24496193793869	31.444034306538665	18.155566159254928
13	23.496369331793872	27.787159029942814	31.052164432060643	17.664307206202672
14	17.996593080838924	29.9620986424243	31.082327286752324	20.95898098998445
15	15.673064323523814	24.236595454309455	40.06344088945808	20.026899332708652
16	17.54093618578341	22.482452388428342	36.22707190379533	23.749539521992922
17	17.592608617181455	20.843768774522772	29.786807954093124	31.77681465420265
18	20.925356824098635	21.805518813462523	33.40066407727625	23.868460285162595
19	23.242210195539357	24.132756118485627	30.132444600478152	22.49258908549686
20	26.660007763226535	24.04251479092444	28.477443376657412	20.82003406919161
21	23.142573880451256	26.09630851161146	29.174155872732534	21.58696173520475
22	20.285755962726622	24.63167940346774	28.28311547675853	26.799449157047107
23	19.543799184613977	26.019417955799057	27.367598665911768	27.069184193675195
24	21.80972183419825	22.056216638522912	31.200259103866536	24.93380242341231
25	19.28444808156827	25.09178655577285	28.49227756748939	27.131487795169495
26	18.459667071310427	28.132301203300113	28.17532035671284	25.23271136867662
27	21.327363395645175	26.66495249350386	29.32225054453842	22.68543356631254
28	16.624924901908912	25.587001293046967	33.8728858187608	23.915187986283318
29	18.3147864741848	24.15698529684452	30.609116599212307	26.919111629758376
30	21.41562683109543	25.198098256735342	28.755584454756956	24.630690457412275
31	22.76084070304175	28.254436041150043	24.5725898766537	24.4121333791545
32	22.450558878139596	27.3915806077568	23.64866702433549	26.50919348976812
33	22.59247263709883	25.208976663345457	24.070205280477463	28.128345419078254
34	19.057732198352912	24.932813477356845	27.82127766885636	28.18817665543389
35	18.647566821848784	24.676429212477533	26.102983897485853	30.57302006818783
36	23.45112504975635	25.320727567613005	25.617164147738652	25.610983234891993
37	20.497884891623876	26.29088364802421	28.48931072932299	24.721920731028924
38	17.3218846344979	26.090869308306402	26.631081091104182	29.956164966091514
39	20.50727987915079	23.749539521992922	26.619460974952467	29.123719623903817
40	21.856944008346705	23.755473198325713	26.54529002079259	27.84229277253499
41	17.6237604179286	25.187467086639092	24.896964182846236	32.29180831258607
42	20.80421093230417	22.85998254510212	25.778609591293318	30.55719693130039
43	22.94527914238598	21.29868396003669	26.98364035987747	28.772396537699862
44	19.973743482227402	23.24814387187215	27.93550093826257	28.84261170763788
45	19.687196362656408	24.31595837526053	24.92514914542699	31.071696116656078
46	21.956827559948675	25.27103302832589	27.361912226092848	25.41022718563259
47	18.027744881586074	24.190362226216465	30.048384185763627	27.733508706433835
48	18.711848315454013	22.196399741885077	28.878708238662355	30.213043703998554
49	20.107745672742915	19.12770013177706	31.621055650466907	29.143498545013113
50	20.022696311972922	21.179021487325418	29.094298478753732	29.703983721947928
51	17.86654667454527	21.42428010908075	25.75339146687896	34.95578174949502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	157.0
2	264.0
3	993.5
4	1723.0
5	1149.5
6	576.0
7	614.5
8	653.0
9	740.0
10	827.0
11	796.0
12	765.0
13	804.5
14	844.0
15	790.5
16	737.0
17	769.5
18	802.0
19	755.5
20	709.0
21	1046.0
22	1383.0
23	1359.5
24	1336.0
25	1837.5
26	2092.5
27	1846.0
28	2038.0
29	2230.0
30	3207.5
31	4185.0
32	4754.5
33	5324.0
34	6668.0
35	8012.0
36	8678.5
37	9345.0
38	10389.5
39	11434.0
40	14676.5
41	17919.0
42	20123.5
43	22328.0
44	24369.5
45	26411.0
46	30032.5
47	33654.0
48	38368.5
49	43083.0
50	48459.5
51	53836.0
52	50183.5
53	46531.0
54	38274.0
55	30017.0
56	25487.0
57	20957.0
58	18233.5
59	15510.0
60	13607.5
61	11705.0
62	10873.5
63	10042.0
64	8132.0
65	6222.0
66	5040.5
67	3859.0
68	3401.0
69	2943.0
70	2270.5
71	1598.0
72	1360.5
73	1123.0
74	874.0
75	472.5
76	320.0
77	256.5
78	193.0
79	168.5
80	144.0
81	100.0
82	56.0
83	30.5
84	5.0
85	3.5
86	2.0
87	2.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	404471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.17184516251223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25414896020808	23.968034416571165
2	11.887564614233822	7.886649054854473
3	4.894519315062959	4.870807105925816
4	2.5042634935791326	3.322841634205557
5	1.4356714244272308	2.381193509767174
6	0.9009334950623206	1.793137583995696
7	0.6457622487627833	1.4994787729428325
8	0.49657879074407635	1.3177947806040047
9	0.34534389580993174	1.0310124815663229
>10	4.0142220728737685	33.55892222535698
>50	0.5439235082328787	11.201867401037708
>100	0.07113985938740648	3.93331315084495
>500	0.0022231206058564526	0.4788509479311269
>1k	0.0037052010097607547	2.756096934396199
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2810	0.6947346039641903	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2803	0.6930039483671264	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2319	0.5733414756558567	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2245	0.5550459736297534	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1035	0.25588979185157895	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	857	0.2118816923833847	RNA PCR Primer, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	573	0.1416665224453669	Illumina PCR Primer Index 7 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	518	0.1280685141827226	Illumina PCR Primer Index 7 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	486	0.12015694573900229	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	465	0.11496497894781085	RNA PCR Primer, Index 16 (95% over 24bp)
TTCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	439	0.10853682958728808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.472365138662599E-4	0.0	0.0	0.7461597988483724	0.0
2	2.472365138662599E-4	0.0	0.0	2.783883146134086	0.0
3	2.472365138662599E-4	0.0	0.0	3.8628232926464445	0.0
4	2.472365138662599E-4	0.0	0.0	4.872042742248517	0.0
5	2.472365138662599E-4	0.0	0.0	6.63483908611495	0.0
6	2.472365138662599E-4	0.0	0.0	7.7194656724462325	0.0
7	2.472365138662599E-4	0.0	0.0	8.800878184097252	0.0
8	2.472365138662599E-4	0.0	0.0	10.638339955151297	0.0
9	2.472365138662599E-4	0.0	0.0	11.690331321652232	0.0
10	2.472365138662599E-4	0.0	0.0	13.410108512105936	0.0
11	2.472365138662599E-4	0.0	0.0	16.066417617085033	0.0
12	2.472365138662599E-4	0.0	0.0	18.44779971864485	0.0
13	2.472365138662599E-4	0.0	0.0	19.236484197878216	0.0
14	2.472365138662599E-4	0.0	0.0	19.501027267715113	0.0
15	2.472365138662599E-4	0.0	0.0	19.953222851576502	0.0
16	2.472365138662599E-4	0.0	0.0	20.919423147765848	0.0
17	4.944730277325198E-4	0.0	0.0	22.48591369962247	0.0
18	4.944730277325198E-4	0.0	0.0	24.317936267371454	0.0
19	4.944730277325198E-4	0.0	0.0	25.254220945382983	0.0
20	4.944730277325198E-4	0.0	0.0	26.287916809857816	0.0
21	4.944730277325198E-4	0.0	0.0	27.53621396836856	0.0
22	4.944730277325198E-4	0.0	0.0	28.711823591802627	0.0
23	4.944730277325198E-4	0.0	0.0	29.850100501642885	0.0
24	4.944730277325198E-4	0.0	0.0	30.73891576899209	0.0
25	4.944730277325198E-4	0.0	0.0	31.481614256646335	0.0
26	4.944730277325198E-4	0.0	0.0	32.153108628307095	0.0
27	4.944730277325198E-4	0.0	0.0	32.784797921235395	0.0
28	4.944730277325198E-4	0.0	0.0	33.475823977491586	0.0
29	4.944730277325198E-4	0.0	0.0	34.20195761871679	0.0
30	4.944730277325198E-4	0.0	0.0	35.11228246277236	0.0
31	7.417095415987796E-4	0.0	0.0	35.87747947318844	0.0
32	7.417095415987796E-4	0.0	0.0	36.57196684063876	0.0
33	7.417095415987796E-4	0.0	0.0	37.19871140328973	0.0
34	7.417095415987796E-4	0.0	0.0	37.90061586615604	0.0
35	7.417095415987796E-4	0.0	0.0	38.69127823750034	0.0
36	7.417095415987796E-4	0.0	0.0	39.35065801998166	0.0
37	7.417095415987796E-4	0.0	0.0	40.004598599157916	0.0
38	7.417095415987796E-4	0.0	0.0	40.673620605680014	0.0
39	7.417095415987796E-4	0.0	0.0	41.44425681940115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	20	7.027858E-4	45.000004	14
TTGGCGA	20	7.027858E-4	45.000004	5
TCGCACT	20	7.027858E-4	45.000004	33
CCCTCGC	20	7.027858E-4	45.000004	44
CCCTCAT	20	7.027858E-4	45.000004	27
CTCAAAT	20	7.027858E-4	45.000004	11
TTTCGAG	20	7.027858E-4	45.000004	1
CGTGAGA	20	7.027858E-4	45.000004	42
GACCGTG	20	7.027858E-4	45.000004	39
CGTGACA	20	7.027858E-4	45.000004	33
GACCGGT	20	7.027858E-4	45.000004	10
CGACCGT	20	7.027858E-4	45.000004	38
GGATCGA	20	7.027858E-4	45.000004	8
ATAGATT	20	7.027858E-4	45.000004	11
ATTCGGA	20	7.027858E-4	45.000004	26
TGAGTAG	20	7.027858E-4	45.000004	28
TAACGGG	20	7.027858E-4	45.000004	3
TAACGCA	20	7.027858E-4	45.000004	10
GCGTTAG	20	7.027858E-4	45.000004	1
ACGGGAT	20	7.027858E-4	45.000004	5
>>END_MODULE