##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933157.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1287843
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21827039476085	33.0	31.0	34.0	30.0	34.0
2	32.40422862103533	34.0	31.0	34.0	30.0	34.0
3	32.47477526375498	34.0	31.0	34.0	30.0	34.0
4	35.94997526872453	37.0	35.0	37.0	35.0	37.0
5	35.17993187057739	37.0	35.0	37.0	35.0	37.0
6	34.86341502807407	37.0	35.0	37.0	32.0	37.0
7	35.65139850121482	37.0	35.0	37.0	35.0	37.0
8	35.939062447829436	37.0	35.0	37.0	35.0	37.0
9	37.79617934794847	39.0	38.0	39.0	35.0	39.0
10	37.30916423818742	39.0	37.0	39.0	34.0	39.0
11	37.21896457875688	39.0	37.0	39.0	34.0	39.0
12	37.06390608171959	39.0	37.0	39.0	33.0	39.0
13	36.998436144778516	39.0	37.0	39.0	33.0	39.0
14	38.14973331376573	40.0	37.0	41.0	33.0	41.0
15	38.25219300799865	40.0	37.0	41.0	33.0	41.0
16	38.23958432821392	40.0	37.0	41.0	34.0	41.0
17	38.204500859188585	40.0	37.0	41.0	34.0	41.0
18	38.15009904157572	40.0	37.0	41.0	34.0	41.0
19	38.13936093141788	40.0	37.0	41.0	34.0	41.0
20	38.051368062721934	40.0	37.0	41.0	34.0	41.0
21	37.94091748761301	40.0	36.0	41.0	33.0	41.0
22	37.965965571890365	40.0	36.0	41.0	33.0	41.0
23	37.89147434896956	40.0	36.0	41.0	33.0	41.0
24	37.82884714984668	40.0	36.0	41.0	33.0	41.0
25	37.7305875017374	40.0	36.0	41.0	33.0	41.0
26	37.658734799195244	40.0	36.0	41.0	33.0	41.0
27	37.600291339860526	40.0	36.0	41.0	33.0	41.0
28	37.50016267510869	39.0	36.0	41.0	33.0	41.0
29	37.455003443742754	39.0	36.0	41.0	33.0	41.0
30	37.379454638492426	39.0	35.0	41.0	32.0	41.0
31	37.30529575421849	39.0	35.0	41.0	32.0	41.0
32	37.288020356518615	39.0	35.0	41.0	32.0	41.0
33	37.19139134195706	39.0	35.0	41.0	32.0	41.0
34	37.08414767949199	39.0	35.0	41.0	32.0	41.0
35	36.9489246748245	39.0	35.0	41.0	31.0	41.0
36	36.80532176670604	39.0	35.0	41.0	31.0	41.0
37	36.74928388010029	39.0	35.0	41.0	31.0	41.0
38	36.67796229819939	39.0	35.0	41.0	31.0	41.0
39	36.63455560965118	39.0	35.0	41.0	31.0	41.0
40	36.50438291002863	39.0	35.0	41.0	30.0	41.0
41	36.36687002996483	39.0	35.0	40.0	30.0	41.0
42	36.30704596755971	38.0	35.0	40.0	30.0	41.0
43	36.20210693384209	38.0	35.0	40.0	30.0	41.0
44	36.1351461319431	38.0	35.0	40.0	30.0	41.0
45	36.123396252493514	38.0	35.0	40.0	30.0	41.0
46	35.8944739382052	38.0	35.0	40.0	29.0	41.0
47	35.810424096726074	38.0	35.0	40.0	29.0	41.0
48	35.72703815604852	38.0	35.0	40.0	29.0	41.0
49	35.77380317321288	38.0	35.0	40.0	29.0	41.0
50	35.574796772587966	37.0	34.0	40.0	29.0	41.0
51	34.45705804201288	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	21.0
10	36.0
11	23.0
12	23.0
13	20.0
14	31.0
15	53.0
16	75.0
17	147.0
18	275.0
19	503.0
20	865.0
21	1424.0
22	2004.0
23	3147.0
24	4665.0
25	6614.0
26	9246.0
27	11812.0
28	13943.0
29	17102.0
30	21412.0
31	27133.0
32	35533.0
33	50252.0
34	85368.0
35	109598.0
36	113288.0
37	168319.0
38	266551.0
39	338237.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.01100134100197	4.644354940780826	54.12857001979279	17.21607369842442
2	21.23038289605177	4.473604313569278	52.71900379161124	21.577008998767706
3	21.23659483337643	4.3176070375037945	52.08259081269999	22.363207316419782
4	19.022893318517863	4.973665268204276	51.08107121753195	24.92237019574591
5	17.843091122132122	7.940564183677669	49.23208807284739	24.98425662134282
6	22.464927790111062	7.6110209086045435	52.64609117726307	17.27796012402133
7	84.14255464369492	2.4101540327508864	8.942860271011295	4.504431052542896
8	85.9756973482016	2.6550596617755424	6.983382291164373	4.38586069885848
9	80.25450307219126	5.2021092633185875	8.981529580857293	5.561858083632865
10	40.582198295910295	26.20272812757456	16.789546551870068	16.425527024645085
11	29.943401486050707	24.641280031805117	24.184547340009612	21.23077114213456
12	26.89279671512754	20.912719951112052	30.869834288806942	21.32464904495346
13	24.17297760674244	22.38681267825348	32.75375958094271	20.68645013406137
14	21.425360078829485	24.630797387569757	31.56549361995212	22.378348913648637
15	19.80885868852026	23.421177892025657	34.544661111641716	22.225302307812363
16	21.68525200664988	21.38793315644842	33.27245634755168	23.65435848935002
17	21.656987691822682	21.55542251656452	29.72272241259222	27.06486737902058
18	23.153055147250093	21.34095538043069	30.968448793835897	24.537540678483325
19	24.584440805284494	23.506359082590038	29.339445879660797	22.56975423246467
20	27.006552817385348	23.14575612089362	28.403384574051337	21.444306487669692
21	26.12942726714359	23.54386365418766	28.081994466716825	22.244714611951924
22	24.396374402780463	22.72054901102075	27.41537594256443	25.467700643634355
23	23.231403206757346	24.0947848456683	27.13816823945155	25.53564370812281
24	23.65614442133086	22.98812821128041	28.852818239490375	24.502909127898352
25	24.266079017395754	23.217892243076214	27.19951112053255	25.316517618995483
26	23.182717147975335	24.387910638175615	27.830876900367514	24.598495313481536
27	22.388520961017765	23.70490812932943	29.247120961173064	24.659449948479743
28	21.421632916434692	23.368997618498526	30.831475575827177	24.377893889239605
29	23.01763491357254	22.242074538588945	29.788413649800482	24.951876898038037
30	23.932730930711273	23.033708301400093	29.02442300808406	24.00913775980457
31	23.829146875822595	25.838009757400553	26.918188008942085	23.414655357834768
32	24.28727725351615	25.988338640657282	26.731053397036753	22.993330708789813
33	23.647603007509453	23.996558586722138	28.215007574681078	24.140830831087328
34	22.30582454538325	23.34430516763301	29.045155348905105	25.304714938078632
35	23.027962259374785	23.113454046805394	28.863766779025084	24.994816914794736
36	24.574346407131927	23.737365501850768	28.43025120298049	23.258036888036816
37	21.932331813738166	25.482842240863214	29.382929440933403	23.201896504465218
38	22.01549412467203	25.24989459118852	28.36215284005892	24.37245844408053
39	23.400756148070844	23.169050885861086	29.986419152023963	23.443773814044103
40	23.933119176794065	22.75921832086675	30.458060493398655	22.849602008940533
41	20.957368250633035	23.808647482651224	30.122460579433984	25.111523687281757
42	21.523586337775644	24.080342091388466	28.95399516866575	25.442076402170137
43	22.229961260805858	21.99266525500391	30.18139633480168	25.59597714938855
44	22.12117470840778	21.994218239335076	29.342940094405918	26.541666957851227
45	23.188540839217204	22.09190095376533	28.466280439463503	26.25327776755396
46	23.360456204677124	22.98292571377101	29.216294222199444	24.440323859352418
47	20.97988652343492	23.802979089842474	31.19860107171449	24.018533315008117
48	21.049460221471094	22.642045653080384	31.396373626288298	24.912120499160224
49	22.21016071058351	21.731686238151703	31.64951007226813	24.408642978996664
50	21.884499896338298	21.84831536142216	30.674158263080205	25.593026479159338
51	20.63481340505015	23.320544507366193	28.810654714899254	27.233987372684403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	965.5
2	1649.0
3	3813.5
4	5978.0
5	4162.0
6	2346.0
7	2271.5
8	2197.0
9	2257.0
10	2317.0
11	2391.5
12	2466.0
13	2546.5
14	2627.0
15	2501.5
16	2376.0
17	2465.5
18	2555.0
19	2601.0
20	2647.0
21	2685.0
22	2723.0
23	3311.5
24	3900.0
25	4798.0
26	7208.0
27	8720.0
28	10646.5
29	12573.0
30	14257.0
31	15941.0
32	18778.0
33	21615.0
34	24241.0
35	26867.0
36	30624.0
37	34381.0
38	38502.5
39	42624.0
40	48635.0
41	54646.0
42	63592.5
43	72539.0
44	85550.5
45	98562.0
46	113750.0
47	128938.0
48	128029.5
49	127121.0
50	114822.5
51	102524.0
52	93602.0
53	84680.0
54	78670.0
55	72660.0
56	71255.5
57	69851.0
58	66266.5
59	62682.0
60	59576.5
61	56471.0
62	51543.5
63	46616.0
64	40666.0
65	34716.0
66	29251.0
67	23786.0
68	21324.5
69	18863.0
70	15530.5
71	12198.0
72	10008.5
73	7819.0
74	6331.0
75	3999.0
76	3155.0
77	2359.5
78	1564.0
79	1097.0
80	630.0
81	461.0
82	292.0
83	213.5
84	135.0
85	83.0
86	31.0
87	22.0
88	13.0
89	19.0
90	25.0
91	13.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1287843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.714553829145064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42334717732376	23.16594456852207
2	10.54947405736586	6.480447776082661
3	3.829829736242232	3.528945347708174
4	1.819325038703079	2.235190273358286
5	1.0157358399994634	1.55989365669277
6	0.6505151689790032	1.198816990456859
7	0.506618764506792	1.089239851930918
8	0.36165286168601496	0.8886405030175568
9	0.2797393630040683	0.7732862750807287
>10	4.7071244441618765	38.67393246469326
>50	0.7804028593983325	14.553315948897508
>100	0.07056026198326171	3.67291926124501
>500	0.0041941414342554495	0.8385501231837763
>1k	0.0014802852120901586	1.3408769591303924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	4047	0.31424637941115496	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3614	0.2806242686414415	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3187	0.2474680531710775	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3138	0.2436632415597243	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2529	0.1963748686757625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6589529934937723E-4	0.0	0.0	0.33847293497732256	0.0
2	4.6589529934937723E-4	0.0	0.0	1.1499848972273794	0.0
3	4.6589529934937723E-4	0.0	0.0	1.646085741817908	0.0
4	4.6589529934937723E-4	0.0	0.0	2.189086713209607	0.0
5	4.6589529934937723E-4	0.0	0.0	3.054331933317959	0.0
6	4.6589529934937723E-4	0.0	0.0	3.682281147624361	0.0
7	4.6589529934937723E-4	0.0	0.0	4.266203256142247	0.0
8	4.6589529934937723E-4	0.0	0.0	5.188831247287131	0.0
9	4.6589529934937723E-4	0.0	0.0	5.702558464036377	0.0
10	4.6589529934937723E-4	0.0	0.0	6.5635329772340265	0.0
11	4.6589529934937723E-4	0.0	0.0	8.16815403740984	0.0
12	4.6589529934937723E-4	0.0	0.0	9.533071966070398	0.0
13	4.6589529934937723E-4	0.0	0.0	9.963403924236106	0.0
14	4.6589529934937723E-4	0.0	0.0	10.109151503715903	0.0
15	6.211937324658363E-4	0.0	0.0	10.340313221409753	0.0
16	6.211937324658363E-4	0.0	0.0	10.945045319965244	0.0
17	6.211937324658363E-4	0.0	0.0	11.760439743043213	0.0
18	6.211937324658363E-4	0.0	0.0	12.642767790794375	0.0
19	6.211937324658363E-4	0.0	0.0	13.247033994050517	0.0
20	6.211937324658363E-4	0.0	0.0	13.878166826235807	0.0
21	6.211937324658363E-4	0.0	0.0	14.74597447049058	0.0
22	6.211937324658363E-4	0.0	0.0	15.657032728368288	0.0
23	6.211937324658363E-4	0.0	0.0	16.520802613362033	0.0
24	6.211937324658363E-4	0.0	0.0	17.21949026395298	0.0
25	6.211937324658363E-4	0.0	0.0	17.81808807440037	0.0
26	6.211937324658363E-4	0.0	0.0	18.349208715658662	0.0
27	6.211937324658363E-4	0.0	0.0	18.861848843376094	0.0
28	7.764921655822953E-4	0.0	0.0	19.407800484997008	0.0
29	7.764921655822953E-4	0.0	0.0	19.996847441807734	0.0
30	7.764921655822953E-4	0.0	0.0	20.62875676615861	0.0
31	8.54141382140525E-4	0.0	0.0	21.230693492918004	0.0
32	8.54141382140525E-4	0.0	0.0	21.782468825780782	0.0
33	8.54141382140525E-4	0.0	0.0	22.377572421483052	0.0
34	9.317905986987545E-4	0.0	0.0	22.95093423654902	0.0
35	9.317905986987545E-4	0.0	0.0	23.565683084040522	0.0
36	9.317905986987545E-4	0.0	0.0	24.1561277267493	0.0
37	9.317905986987545E-4	0.0	0.0	24.730110735547733	0.0
38	9.317905986987545E-4	0.0	0.0	25.264104397818677	0.0
39	9.317905986987545E-4	0.0	0.0	25.839718040164833	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	35	1.2120836E-7	45.000004	1
ACACGAA	25	3.8910686E-5	45.0	28
ACGTTAT	20	7.033604E-4	45.0	20
CACTACG	25	3.8910686E-5	45.0	30
CGTACCG	20	7.033604E-4	45.0	45
CACGTTA	20	7.033604E-4	45.0	19
AATCGGC	20	7.033604E-4	45.0	33
TGCGTAT	30	2.165707E-6	44.999996	33
CGAATAT	155	0.0	43.548386	14
TTGTGCG	255	0.0	40.588234	1
CTACGAA	175	0.0	39.857143	11
TTTGGGC	3315	0.0	39.84163	4
TCGTTTG	460	0.0	39.619568	1
CGTACAT	40	3.4586628E-7	39.375	35
ATCGTTC	35	6.2488743E-6	38.57143	11
GCGATAG	35	6.2488743E-6	38.57143	9
GCGTATG	65	9.094947E-12	38.07692	1
TTGGGCA	1335	0.0	37.58427	5
TACGAAT	180	0.0	37.5	12
CGAACGA	30	1.1399985E-4	37.499996	22
>>END_MODULE