SRR2933156.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368303
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3143	0.22970058532357235	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2909	0.21259910999245052	TruSeq Adapter, Index 27 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2901	0.2120144441691643	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2891	0.2112836118900565	TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2807	0.20514462074555126	TruSeq Adapter, Index 27 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2181	0.1593945200734048	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	25	3.891196E-5	45.000004	36
ACCGAAA	20	7.033757E-4	45.000004	20
CTACGAT	20	7.033757E-4	45.000004	22
GCGTAAA	25	3.891196E-5	45.000004	39
ACGCGTC	25	3.891196E-5	45.000004	35
GTCGATA	30	2.165807E-6	44.999996	25
ACGATTA	30	2.165807E-6	44.999996	12
CGACTAA	30	2.165807E-6	44.999996	13
TACGAAT	400	0.0	41.625004	12
CGGTAGT	200	0.0	41.625004	12
TTATGCG	95	0.0	40.263157	1
CGAATAT	410	0.0	40.060974	14
CACGACC	290	0.0	39.568966	27
TACCGAT	40	3.458863E-7	39.375004	27
TATGGGA	955	0.0	39.34555	4
GCTACGA	425	0.0	39.17647	10
TTTGGGA	6925	0.0	38.95668	4
TTTGGGC	3760	0.0	38.776596	4
TTGGGAC	2810	0.0	38.674377	5
GACCTAT	355	0.0	38.661972	33