##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368303
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20884409374239	33.0	31.0	34.0	30.0	34.0
2	32.402236931439894	34.0	31.0	34.0	30.0	34.0
3	32.478491971442	34.0	31.0	34.0	30.0	34.0
4	35.94438439439218	37.0	35.0	37.0	35.0	37.0
5	35.152579509070726	37.0	35.0	37.0	35.0	37.0
6	34.831144125241266	37.0	35.0	37.0	32.0	37.0
7	35.61639636834824	37.0	35.0	37.0	33.0	37.0
8	35.899097641384984	37.0	35.0	37.0	35.0	37.0
9	37.76101784473176	39.0	38.0	39.0	35.0	39.0
10	37.274463331586645	39.0	37.0	39.0	34.0	39.0
11	37.216888364638535	39.0	37.0	39.0	34.0	39.0
12	37.066046774727525	39.0	37.0	39.0	33.0	39.0
13	37.002060216194806	39.0	37.0	39.0	33.0	39.0
14	38.18070632016447	40.0	37.0	41.0	33.0	41.0
15	38.26597398383253	40.0	37.0	41.0	33.0	41.0
16	38.26024060460293	40.0	37.0	41.0	34.0	41.0
17	38.212517987609466	40.0	37.0	41.0	34.0	41.0
18	38.17292003306285	40.0	37.0	41.0	34.0	41.0
19	38.163308857760306	40.0	37.0	41.0	34.0	41.0
20	38.08779122752782	40.0	37.0	41.0	34.0	41.0
21	37.948376200300665	40.0	36.0	41.0	33.0	41.0
22	37.97953742701726	40.0	37.0	41.0	33.0	41.0
23	37.90964135867567	40.0	36.0	41.0	33.0	41.0
24	37.82533181612552	40.0	36.0	41.0	33.0	41.0
25	37.70952632567494	40.0	36.0	41.0	33.0	41.0
26	37.65513267163779	40.0	36.0	41.0	33.0	41.0
27	37.60522413529752	40.0	36.0	41.0	33.0	41.0
28	37.52399139664241	40.0	36.0	41.0	33.0	41.0
29	37.460369523416965	39.0	36.0	41.0	33.0	41.0
30	37.37575010798047	39.0	36.0	41.0	32.0	41.0
31	37.32753344836633	39.0	35.0	41.0	32.0	41.0
32	37.32020539310372	39.0	35.0	41.0	32.0	41.0
33	37.21672831236941	39.0	35.0	41.0	32.0	41.0
34	37.11096080327238	39.0	35.0	41.0	32.0	41.0
35	36.9732639627334	39.0	35.0	41.0	31.0	41.0
36	36.82642660287963	39.0	35.0	41.0	31.0	41.0
37	36.785999153696224	39.0	35.0	41.0	31.0	41.0
38	36.680067207336386	39.0	35.0	41.0	31.0	41.0
39	36.630806188395404	39.0	35.0	41.0	31.0	41.0
40	36.52030873278799	39.0	35.0	41.0	30.0	41.0
41	36.41065465763066	39.0	35.0	41.0	30.0	41.0
42	36.314458128060814	39.0	35.0	40.0	30.0	41.0
43	36.1555956538866	38.0	35.0	40.0	30.0	41.0
44	36.0697659801959	38.0	35.0	40.0	30.0	41.0
45	36.0566972373809	38.0	35.0	40.0	30.0	41.0
46	35.85007341210244	38.0	35.0	40.0	29.0	41.0
47	35.773884147005454	38.0	35.0	40.0	29.0	41.0
48	35.67843891301853	38.0	35.0	40.0	29.0	41.0
49	35.72114728974503	38.0	35.0	40.0	29.0	41.0
50	35.52728452689207	37.0	34.0	40.0	29.0	41.0
51	34.49816086056963	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	25.0
10	22.0
11	27.0
12	34.0
13	25.0
14	35.0
15	55.0
16	100.0
17	158.0
18	317.0
19	550.0
20	953.0
21	1518.0
22	2384.0
23	3422.0
24	5070.0
25	7260.0
26	10172.0
27	12617.0
28	15242.0
29	18299.0
30	22999.0
31	29210.0
32	37733.0
33	52914.0
34	88563.0
35	113017.0
36	122021.0
37	179593.0
38	280351.0
39	363444.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.82169519470468	4.509746744690321	53.06412395500119	17.604434105603804
2	21.00039245693388	4.684707992308721	53.15767048672699	21.157229064030407
3	21.673342819536316	4.608482185597781	51.62811160978234	22.090063385083567
4	18.930967775412316	5.0777495920128795	51.34644884941421	24.644833783160603
5	17.77610660796622	8.129997522478574	49.49437368769929	24.59952218185592
6	22.02553089483835	7.765312215203796	52.74146150377511	17.46769538618274
7	84.55656386048996	2.3926717985709307	8.707501189429534	4.3432631515095705
8	86.57475719924608	2.8533884673204692	6.585018084444746	3.986836248988711
9	81.05127300020536	5.526480611385051	8.47385410979878	4.948392278610805
10	39.2267648320584	27.687580893997893	16.245597649058723	16.840056624884987
11	30.22817314586024	24.384657491798233	24.167307971991583	21.219861390349944
12	27.437490088087213	20.723260856696214	30.639339386086267	21.1999096691303
13	23.90070035657307	22.98832933933493	31.3611093449331	21.7498609591589
14	21.002877286682846	24.969688731224004	30.76643111942311	23.26100286267004
15	19.707038572596858	22.994833746618987	34.55280007425256	22.745327606531593
16	21.641405448939306	21.30120302301464	32.09442645378984	24.962965074256214
17	21.103951390883452	21.942216015020065	29.74311976221641	27.210712831880073
18	22.632706352321087	21.624377056836096	30.740998156110162	25.001918434732655
19	24.29578828665873	23.867447487873665	27.56794364990795	24.26882057555965
20	27.898133673608843	22.098175623381664	28.185862341893568	21.817828361115925
21	26.488212040754135	24.03729290953831	27.077482107398726	22.39701294230883
22	25.01149233758897	23.543031039177727	25.954485227321726	25.49099139591158
23	24.631898051820393	24.521907793814673	25.808172605044348	25.038021549320582
24	23.62817299969378	22.479012324024723	29.22751758930588	24.665297086975617
25	23.79370651091169	23.79816458781425	26.83214171130225	25.575987189971812
26	22.70250083497588	25.05570769047499	26.28394441874351	25.957847055805622
27	22.29944683304794	24.967203901475038	27.15275783214683	25.58059143333019
28	21.207583408060934	25.004695597393265	28.99657458910782	24.791146405437978
29	21.911813392209183	22.873881004426654	28.522629856106434	26.691675747257737
30	22.20114989150795	24.108475973523408	28.54046216371666	25.14991197125198
31	22.845743961681002	25.464023684812503	26.39905050270298	25.29118185080351
32	22.931105171880787	25.236515596326253	25.864885189903113	25.967494041889843
33	22.652292657401173	25.139168736749095	25.899234307021178	26.30930429882855
34	21.738313809148995	23.166286999297668	28.144789567807717	26.95060962374562
35	21.851008146587414	23.512701499594755	27.062426962449106	27.573863391368725
36	22.172793599078567	25.767026747730583	26.08545037173784	25.974729281453012
37	21.891569338077897	26.072222307485987	27.261578758505973	24.774629595930143
38	20.814395641900955	25.338028199894318	28.003373521800363	25.84420263640436
39	21.548224333353065	23.734582179531873	29.000301833731275	25.71689165338379
40	22.60106131463572	23.11827131856029	30.487545521715585	23.793121845088404
41	20.228048904372788	23.75321840264912	29.495879202194253	26.522853490783838
42	20.782604437759765	23.954781945227044	27.50377657580229	27.7588370412109
43	21.625400222026848	23.38926392765345	27.724195591181193	27.26114025913851
44	21.493119579508342	23.539011461642634	27.669675503159752	27.298193455689272
45	21.6745852344108	23.297471393397515	27.188422447367287	27.839520924824402
46	21.62481555620356	24.414767781697474	28.429594906976014	25.53082175512295
47	19.941124151595076	24.538716936234152	29.735372940057868	25.7847859721129
48	19.87527616324747	22.723402638158362	30.080910441620023	27.32041075697415
49	20.468419640971337	23.678381177268484	29.694373249199923	26.15882593256026
50	20.34666298327198	22.88403957310625	29.714544220103296	27.054753223518475
51	19.491223800576336	23.48975336603077	28.37258998920561	28.646432844187288
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	264.0
1	975.5
2	1687.0
3	3869.5
4	6052.0
5	4270.5
6	2489.0
7	2368.5
8	2248.0
9	2352.5
10	2457.0
11	2507.0
12	2557.0
13	2644.0
14	2731.0
15	2566.5
16	2402.0
17	2495.0
18	2588.0
19	2784.5
20	2981.0
21	2993.0
22	3005.0
23	3578.0
24	4151.0
25	5106.5
26	8109.5
27	10157.0
28	12357.5
29	14558.0
30	16576.5
31	18595.0
32	22465.0
33	26335.0
34	30987.5
35	35640.0
36	37595.5
37	39551.0
38	42549.5
39	45548.0
40	51212.0
41	56876.0
42	63376.5
43	69877.0
44	76392.5
45	82908.0
46	92522.5
47	102137.0
48	113270.0
49	124403.0
50	122611.0
51	120819.0
52	108505.0
53	96191.0
54	89293.0
55	82395.0
56	79337.5
57	76280.0
58	73920.0
59	71560.0
60	68873.0
61	66186.0
62	59812.0
63	53438.0
64	48265.5
65	43093.0
66	36219.5
67	29346.0
68	25281.0
69	21216.0
70	18214.5
71	15213.0
72	12616.0
73	10019.0
74	8479.5
75	5326.0
76	3712.0
77	2754.5
78	1797.0
79	1399.5
80	1002.0
81	774.0
82	546.0
83	375.5
84	205.0
85	132.0
86	59.0
87	35.5
88	12.0
89	10.0
90	8.0
91	4.5
92	1.0
93	1.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1368303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.232134472090053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40676655478111	22.335328745069543
2	10.199160875762825	5.962864844455574
3	3.601142912004454	3.158072815707845
4	1.5890891935811553	1.8580987597963794
5	0.8467502988499116	1.2376159300131528
6	0.5829807243739781	1.0225062557721958
7	0.3979032605606147	0.8142093133713684
8	0.31564428144300527	0.7381564864390536
9	0.26106792539380314	0.6868415431315106
>10	4.629809282979744	37.06592825391522
>50	1.0855989371340238	19.4012257919087
>100	0.07968591722833016	3.933095479347231
>500	0.002688788609913358	0.5076068278419354
>1k	0.0017110472972175917	1.2784489532302798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3143	0.22970058532357235	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2909	0.21259910999245052	TruSeq Adapter, Index 27 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2901	0.2120144441691643	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2891	0.2112836118900565	TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2807	0.20514462074555126	TruSeq Adapter, Index 27 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2181	0.1593945200734048	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1924968373233122E-4	0.0	0.0	0.23211233184462798	0.0
2	2.1924968373233122E-4	0.0	0.0	0.9462816349887415	0.0
3	2.1924968373233122E-4	0.0	0.0	1.3581056242659704	0.0
4	2.1924968373233122E-4	0.0	0.0	1.8149488819362378	0.0
5	2.1924968373233122E-4	0.0	0.0	2.5579129768771973	0.0
6	2.1924968373233122E-4	0.0	0.0	3.098582696961126	0.0
7	2.1924968373233122E-4	0.0	0.0	3.589555822065727	0.0
8	2.1924968373233122E-4	0.0	0.0	4.369865446469094	0.0
9	2.1924968373233122E-4	0.0	0.0	4.804564486082396	0.0
10	2.923329116431083E-4	0.0	0.0	5.554763820586522	0.0
11	2.923329116431083E-4	0.0	0.0	6.738858279196933	0.0
12	2.923329116431083E-4	0.0	0.0	7.833645033300373	0.0
13	2.923329116431083E-4	0.0	0.0	8.167635384852623	0.0
14	2.923329116431083E-4	0.0	0.0	8.282887635267919	7.308322791077707E-5
15	3.6541613955388535E-4	0.0	0.0	8.473269443975493	7.308322791077707E-5
16	4.3849936746466245E-4	0.0	0.0	8.950941421600332	7.308322791077707E-5
17	4.3849936746466245E-4	0.0	0.0	9.601382150006248	7.308322791077707E-5
18	4.3849936746466245E-4	0.0	0.0	10.306123716749871	7.308322791077707E-5
19	5.115825953754395E-4	0.0	0.0	10.764136306066712	7.308322791077707E-5
20	5.115825953754395E-4	0.0	0.0	11.239469620398406	7.308322791077707E-5
21	5.115825953754395E-4	0.0	0.0	11.926086546620157	7.308322791077707E-5
22	5.846658232862166E-4	0.0	0.0	12.638574935522323	7.308322791077707E-5
23	5.846658232862166E-4	0.0	0.0	13.349236243726718	7.308322791077707E-5
24	6.577490511969937E-4	0.0	0.0	13.909638435346556	7.308322791077707E-5
25	6.577490511969937E-4	0.0	0.0	14.397249731967262	7.308322791077707E-5
26	6.577490511969937E-4	0.0	0.0	14.813239465235405	7.308322791077707E-5
27	6.577490511969937E-4	0.0	0.0	15.232006361164157	7.308322791077707E-5
28	7.308322791077707E-4	0.0	0.0	15.685122374210975	7.308322791077707E-5
29	7.308322791077707E-4	0.0	0.0	16.159286356896096	7.308322791077707E-5
30	7.308322791077707E-4	0.0	0.0	16.696228832356574	7.308322791077707E-5
31	7.308322791077707E-4	0.0	0.0	17.191733117591646	7.308322791077707E-5
32	8.039155070185478E-4	0.0	0.0	17.66721259837916	7.308322791077707E-5
33	8.039155070185478E-4	0.0	0.0	18.148904153539092	7.308322791077707E-5
34	8.039155070185478E-4	0.0	0.0	18.617367644447175	7.308322791077707E-5
35	8.039155070185478E-4	0.0	0.0	19.143055302809394	7.308322791077707E-5
36	8.039155070185478E-4	0.0	0.0	19.63651325766296	7.308322791077707E-5
37	8.039155070185478E-4	0.0	0.0	20.097960758691606	7.308322791077707E-5
38	8.039155070185478E-4	0.0	0.0	20.570955409730153	7.308322791077707E-5
39	8.039155070185478E-4	0.0	0.0	21.02926033195864	7.308322791077707E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	25	3.891196E-5	45.000004	36
ACCGAAA	20	7.033757E-4	45.000004	20
CTACGAT	20	7.033757E-4	45.000004	22
GCGTAAA	25	3.891196E-5	45.000004	39
ACGCGTC	25	3.891196E-5	45.000004	35
GTCGATA	30	2.165807E-6	44.999996	25
ACGATTA	30	2.165807E-6	44.999996	12
CGACTAA	30	2.165807E-6	44.999996	13
TACGAAT	400	0.0	41.625004	12
CGGTAGT	200	0.0	41.625004	12
TTATGCG	95	0.0	40.263157	1
CGAATAT	410	0.0	40.060974	14
CACGACC	290	0.0	39.568966	27
TACCGAT	40	3.458863E-7	39.375004	27
TATGGGA	955	0.0	39.34555	4
GCTACGA	425	0.0	39.17647	10
TTTGGGA	6925	0.0	38.95668	4
TTTGGGC	3760	0.0	38.776596	4
TTGGGAC	2810	0.0	38.674377	5
GACCTAT	355	0.0	38.661972	33
>>END_MODULE