##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933155.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1852171
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22894214411088	33.0	31.0	34.0	30.0	34.0
2	32.48149927841436	34.0	31.0	34.0	31.0	34.0
3	32.424089352441	34.0	31.0	34.0	30.0	34.0
4	36.0067493768124	37.0	35.0	37.0	35.0	37.0
5	35.23964741916378	37.0	35.0	37.0	35.0	37.0
6	35.01077924230538	37.0	35.0	37.0	33.0	37.0
7	35.765623152505896	37.0	35.0	37.0	35.0	37.0
8	36.02882563219055	37.0	35.0	37.0	35.0	37.0
9	37.895035069656096	39.0	38.0	39.0	35.0	39.0
10	37.48378200500925	39.0	37.0	39.0	35.0	39.0
11	37.35764138408387	39.0	37.0	39.0	34.0	39.0
12	37.30635832220675	39.0	37.0	39.0	34.0	39.0
13	37.34311788706334	39.0	37.0	39.0	34.0	39.0
14	38.58718984370233	40.0	38.0	41.0	34.0	41.0
15	38.6450322351446	40.0	38.0	41.0	34.0	41.0
16	38.59893713917344	40.0	38.0	41.0	34.0	41.0
17	38.50933472125414	40.0	38.0	41.0	34.0	41.0
18	38.26407874866845	39.0	38.0	41.0	34.0	41.0
19	38.06276580294152	39.0	37.0	41.0	34.0	41.0
20	37.78389090424157	39.0	35.0	41.0	34.0	41.0
21	37.66666360719394	39.0	35.0	41.0	33.0	41.0
22	37.70481127282524	39.0	35.0	41.0	33.0	41.0
23	37.628250847248985	39.0	35.0	41.0	33.0	41.0
24	37.56914129418936	39.0	35.0	41.0	33.0	41.0
25	37.48104521666736	39.0	35.0	41.0	33.0	41.0
26	37.463391339136614	39.0	35.0	41.0	33.0	41.0
27	37.43040518397059	39.0	35.0	41.0	33.0	41.0
28	37.32894370984105	39.0	35.0	41.0	33.0	41.0
29	37.22657897138007	39.0	35.0	41.0	33.0	41.0
30	37.04580192649599	39.0	35.0	41.0	33.0	41.0
31	36.80418492676972	39.0	35.0	41.0	31.0	41.0
32	36.43399286566953	39.0	35.0	41.0	30.0	41.0
33	35.900813153861066	39.0	35.0	41.0	27.0	41.0
34	35.402875868372846	39.0	35.0	41.0	23.0	41.0
35	35.06032920286518	39.0	35.0	41.0	21.0	41.0
36	34.84086998446688	39.0	35.0	41.0	20.0	41.0
37	34.7909156335997	39.0	35.0	41.0	18.0	41.0
38	34.67331418103404	39.0	34.0	41.0	18.0	41.0
39	34.60268733286505	39.0	34.0	41.0	18.0	41.0
40	34.51177294105134	39.0	34.0	41.0	18.0	41.0
41	34.366075270587864	38.0	34.0	41.0	17.0	41.0
42	34.24485644144088	38.0	34.0	40.0	16.0	41.0
43	34.12696019967919	38.0	34.0	40.0	15.0	41.0
44	34.053878934504425	38.0	34.0	40.0	15.0	41.0
45	34.06296988776954	38.0	34.0	40.0	15.0	41.0
46	33.88508836387137	38.0	33.0	40.0	15.0	41.0
47	33.826758436451065	38.0	33.0	40.0	15.0	41.0
48	33.729499598039276	38.0	33.0	40.0	15.0	41.0
49	33.76922379197169	38.0	33.0	40.0	15.0	41.0
50	33.59251764550897	38.0	33.0	40.0	15.0	41.0
51	32.68964960578694	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	27.0
11	17.0
12	11.0
13	18.0
14	23.0
15	49.0
16	111.0
17	224.0
18	601.0
19	1181.0
20	2193.0
21	3637.0
22	5734.0
23	9289.0
24	16571.0
25	30700.0
26	46197.0
27	49717.0
28	44211.0
29	39054.0
30	37885.0
31	40727.0
32	47572.0
33	61963.0
34	98255.0
35	123124.0
36	136087.0
37	196610.0
38	349035.0
39	511120.0
40	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.764659418595798	3.9561681939734505	47.599978619684684	27.679193767746067
2	30.71708821701668	3.509017255966107	45.811752802522015	19.9621417244952
3	16.96706189655275	3.720930734797165	58.2750728739409	21.03693449470918
4	14.933718322984216	3.968694035269962	58.45075859626352	22.646829045482303
5	15.129596565327933	6.9405578642576735	56.84734292891963	21.082502641494766
6	18.504716897089956	6.203800836963758	58.90714194315751	16.38434032278877
7	73.87168895312581	1.2748282960914517	20.69727903093181	4.156203719850922
8	76.41486666187949	1.5624907203492548	18.628949486845436	3.3936931309258163
9	70.60849133260373	4.454340338985979	20.490602649539376	4.446565678870903
10	32.07824763480262	28.147994974546087	27.029037815622853	12.74471957502844
11	22.363161932672522	21.783841772708893	38.54444325065018	17.308553043968402
12	19.56736176087413	20.835873145622084	40.778254275658135	18.818510817845652
13	19.521469669917085	20.532121494181695	42.29587872825997	17.65053010764125
14	17.45416594904034	22.17559825739632	42.01971632208905	18.350519471474286
15	16.886507779249325	22.16064283481385	42.81537719789372	18.137472188043112
16	19.49452831299054	22.467255993102146	40.69716025140227	17.341055442505038
17	19.018276390246903	22.36807508593969	40.23030270963102	18.383345814182384
18	19.600242094277473	21.3619044893803	40.15482371768049	18.883029698661733
19	19.19547385203634	24.125310244032544	37.98105034578341	18.698165558147707
20	20.751971605213555	24.68854117681359	36.74007421560968	17.81941300236317
21	20.764443455814824	25.25576742104266	36.80248745931126	17.177301663831255
22	19.823709581890657	22.694070903820435	37.99012078258433	19.492098731704576
23	17.793929394208202	24.227784583604862	37.996491684623074	19.981794337563862
24	18.469029047533947	22.828777688453172	39.144603818977835	19.557589445035042
25	19.10925071173234	24.523869556320662	36.676689139393716	19.69019059255328
26	18.52377561251094	24.305801138231836	36.91511204958937	20.25531119966785
27	18.367634521866503	23.300440402101103	37.68491138237236	20.647013693660035
28	17.793011552389064	24.34974956416011	37.13857953720256	20.718659346248266
29	18.877630629137375	23.6772954549013	36.226730685233704	21.218343230727616
30	19.763240003217845	23.14332747894228	36.784886492661855	20.30854602517802
31	20.03508315376928	24.380470269753708	34.76520256498995	20.819244011487058
32	20.246672688428877	25.37638263421682	33.9984267111406	20.378517966213703
33	20.686480891883093	25.258682918585812	32.40165189931167	21.653184290219425
34	19.945998506617368	25.880709718487115	32.918774778354695	21.254516996540815
35	20.191332225804203	27.126868955404227	31.845601728998023	20.836197089793547
36	19.89308762527866	29.491607416377864	29.837471810108244	20.777833148235235
37	20.72864762486833	29.129113888512453	29.897077537657164	20.245160948962056
38	20.487417198520006	29.012224033310098	30.972680168299792	19.5276785998701
39	21.13136422069021	27.60690022681491	30.287700217744472	20.97403533475041
40	21.952832648821303	26.57821551033895	30.90643358523592	20.56251825560383
41	19.787805769553675	27.44579199220806	31.188966893445585	21.57743534479268
42	19.833319925644012	28.26391299723406	30.861027410536067	21.04173966658586
43	19.982064291040082	26.557267120584438	31.91233422831909	21.548334360056387
44	20.839760475679622	26.177766523717306	31.30861027410536	21.673862726497713
45	21.1301224346996	24.98160267059575	30.907405417750304	22.98086947695434
46	20.894345068570882	25.49753775434342	30.748402820257958	22.859714356827745
47	19.826841042214784	26.201846373795938	31.878158118229905	22.093154465759373
48	19.458732482044045	25.676246955599673	32.32935835838052	22.535662203975768
49	20.742577224241174	24.590656046336974	32.192168001766575	22.474598727655277
50	19.608772624125958	24.409031347537567	33.125073224880424	22.857122803456054
51	18.844750295734034	23.53152057774363	33.04543694939614	24.578292177126194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	1021.0
2	1782.0
3	34650.0
4	67518.0
5	45620.5
6	23723.0
7	22931.5
8	22140.0
9	22138.5
10	22137.0
11	21796.5
12	21456.0
13	20648.0
14	19840.0
15	19050.0
16	18260.0
17	17250.5
18	16241.0
19	15218.0
20	14195.0
21	13919.5
22	13644.0
23	13823.5
24	14003.0
25	15344.0
26	18200.0
27	19715.0
28	23066.5
29	26418.0
30	29780.5
31	33143.0
32	37645.5
33	42148.0
34	47487.5
35	52827.0
36	58429.0
37	64031.0
38	68067.5
39	72104.0
40	77615.5
41	83127.0
42	91062.5
43	98998.0
44	110717.5
45	122437.0
46	139760.5
47	157084.0
48	165161.5
49	173239.0
50	165578.0
51	157917.0
52	140293.5
53	122670.0
54	108604.0
55	94538.0
56	84338.0
57	74138.0
58	66691.0
59	59244.0
60	52032.0
61	44820.0
62	38759.5
63	32699.0
64	26342.0
65	19985.0
66	15903.0
67	11821.0
68	9393.5
69	6966.0
70	5309.5
71	3653.0
72	3064.0
73	2475.0
74	2163.5
75	1526.5
76	1201.0
77	932.0
78	663.0
79	454.0
80	245.0
81	160.5
82	76.0
83	53.5
84	31.0
85	22.5
86	14.0
87	9.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1852171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24291890277375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44876002214528	26.619974529919016
2	7.941735232246011	5.756633319391865
3	3.458628453429026	3.7605237165736196
4	2.186216251391443	3.1693943321242446
5	1.8265097964408432	3.309902321376364
6	1.4858673858020433	3.2311302698339937
7	1.2825586354956842	3.2538568030025568
8	1.114368214226378	3.2310365452828336
9	0.989534119288551	3.227724435321234
>10	6.1281121882331195	34.49060563129451
>50	0.08546950076642963	2.1709832416511627
>100	0.04724358226856105	3.1268037329937535
>500	0.0027254281454681026	0.6522056921247351
>1k	0.002119777446475191	1.9126062252943536
>5k	0.0	0.0
>10k+	1.514126747482279E-4	2.086619203815812
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38024	2.0529421959419514	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT	4054	0.2188782785174803	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT	3926	0.21196746952630183	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	3786	0.20440877219220038	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	3240	0.1749298525892048	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	2805	0.1514439001582467	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	2629	0.14194153779537633	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2341	0.1263922175652248	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGT	2132	0.11510816225931624	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1902	0.10269030235329243	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	1893	0.10220438609610019	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.399069524358172E-5	0.0	0.0	0.16386176006427053	0.0
2	5.399069524358172E-5	0.0	0.0	0.5239796973389605	0.0
3	5.399069524358172E-5	0.0	0.0	0.9568770918019989	0.0
4	5.399069524358172E-5	0.0	0.0	1.405269815799945	0.0
5	5.399069524358172E-5	0.0	0.0	2.3317501461798074	0.0
6	5.399069524358172E-5	0.0	0.0	3.8531539474486967	0.0
7	5.399069524358172E-5	0.0	0.0	5.151036270409158	0.0
8	5.399069524358172E-5	0.0	0.0	6.7189260602827705	0.0
9	5.399069524358172E-5	0.0	0.0	7.929613410424848	0.0
10	5.399069524358172E-5	0.0	0.0	9.129286658737234	0.0
11	5.399069524358172E-5	0.0	0.0	10.370964667949126	0.0
12	5.399069524358172E-5	0.0	0.0	11.383992082804449	0.0
13	1.6197208573074518E-4	0.0	0.0	11.819966946896372	0.0
14	1.6197208573074518E-4	0.0	0.0	12.054394545644003	0.0
15	1.6197208573074518E-4	0.0	0.0	12.24908499269236	0.0
16	1.6197208573074518E-4	0.0	0.0	12.544630058455725	0.0
17	1.6197208573074518E-4	0.0	0.0	12.961762169907638	0.0
18	1.6197208573074518E-4	0.0	0.0	13.437798129870298	0.0
19	1.6197208573074518E-4	0.0	0.0	13.767465315027609	0.0
20	1.6197208573074518E-4	0.0	0.0	14.106310918376327	0.0
21	1.6197208573074518E-4	0.0	0.0	14.486891329148335	0.0
22	1.6197208573074518E-4	0.0	0.0	14.926915495383525	0.0
23	1.6197208573074518E-4	0.0	0.0	15.363592238513615	0.0
24	1.6197208573074518E-4	0.0	0.0	15.705893246357922	0.0
25	2.159627809743269E-4	0.0	0.0	16.01094067448416	0.0
26	2.159627809743269E-4	0.0	0.0	16.30653973094277	0.0
27	2.159627809743269E-4	0.0	0.0	16.61045335446889	0.0
28	2.699534762179086E-4	0.0	0.0	16.91404303382355	0.0
29	3.2394417146149035E-4	0.0	0.0	17.256829957925053	0.0
30	3.2394417146149035E-4	0.0	0.0	17.669642813757477	0.0
31	3.2394417146149035E-4	0.0	0.0	18.008326445020465	0.0
32	3.7793486670507204E-4	0.0	0.0	18.34965562035039	0.0
33	3.7793486670507204E-4	0.0	0.0	18.674733596411993	0.0
34	3.7793486670507204E-4	0.0	0.0	19.021947757523467	0.0
35	3.7793486670507204E-4	0.0	0.0	19.38676288528435	0.0
36	3.7793486670507204E-4	0.0	0.0	19.731763427890836	0.0
37	3.7793486670507204E-4	0.0	0.0	20.093501086022833	0.0
38	3.7793486670507204E-4	0.0	0.0	20.47964253840493	0.0
39	3.7793486670507204E-4	0.0	0.0	20.914861532763442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	24515	0.0	44.311646	1
TTCCGCG	130	0.0	41.538464	1
GCGCGAC	50	1.0822987E-9	40.5	9
ACGGGCG	85	0.0	39.705883	5
CGTTATT	675	0.0	39.666668	1
GTTTTTT	28055	0.0	39.185528	2
TGGGCGA	2640	0.0	39.119316	6
CGAATAT	145	0.0	38.793106	14
ACTCGCG	35	6.2503586E-6	38.571426	1
AGGGCGA	305	0.0	38.360657	6
TTGGGCG	1860	0.0	38.225807	5
GCGATGT	515	0.0	38.00971	9
CACGACC	630	0.0	37.857143	27
TATTGCG	125	0.0	37.8	1
TAGGGCG	210	0.0	37.500004	5
TCTACGG	180	0.0	37.5	2
CTATGCG	60	1.5643309E-10	37.499996	1
CTACGCG	30	1.1401916E-4	37.499996	1
GCGAGAC	655	0.0	37.44275	21
ACGACCA	640	0.0	37.26563	28
>>END_MODULE