FastQCFastQC Report
Sat 14 Jan 2017
SRR2933154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157842
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62320.2888070581627385No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT36680.1699846420636914TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC35940.16655528996098878TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC32660.15135491847873941TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC26940.12484695357676791TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA207.034665E-445.028
TACGGGA3800.040.8552634
CGTATGG1700.039.7058832
TTTGGGC56550.038.2360764
CGTTTTT36450.037.9012341
TTTGGGA113800.037.763624
TTACGGG5200.037.6442343
GCAACGA601.5643309E-1037.5000049
CGTTTGG15550.037.4758872
TATGGGA10400.037.211544
TCGATTG850.037.0588231
ACACGCG4150.036.325336
TTGGGAT52100.036.2332045
TTGGGCA27950.036.22545
TGGGCGA43250.036.104056
GGGCGAT63600.036.0849087
TTGGGAC50450.036.0802765
ATCTCGA250.002107517336.045
CGAAACG250.002107517336.019
TCGAACG250.002107517336.034
Produced by FastQC (version 0.11.5)