##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933154.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2157842
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.312364389978505	33.0	31.0	34.0	31.0	34.0
2	32.501730895959945	34.0	31.0	34.0	31.0	34.0
3	32.56894202633928	34.0	31.0	34.0	31.0	34.0
4	36.0231439558596	37.0	35.0	37.0	35.0	37.0
5	35.238640271159795	37.0	35.0	37.0	35.0	37.0
6	34.91305943623305	37.0	35.0	37.0	32.0	37.0
7	35.68872234389728	37.0	35.0	37.0	35.0	37.0
8	35.97176855395344	37.0	35.0	37.0	35.0	37.0
9	37.844492321495274	39.0	38.0	39.0	35.0	39.0
10	37.38238758908206	39.0	37.0	39.0	35.0	39.0
11	37.312711032596454	39.0	37.0	39.0	34.0	39.0
12	37.15053094712217	39.0	37.0	39.0	34.0	39.0
13	37.11705583634019	39.0	37.0	39.0	33.0	39.0
14	38.31279722982498	40.0	38.0	41.0	34.0	41.0
15	38.40248127527409	40.0	38.0	41.0	34.0	41.0
16	38.38553795875694	40.0	38.0	41.0	34.0	41.0
17	38.33629987737749	40.0	37.0	41.0	34.0	41.0
18	38.27456968582501	40.0	37.0	41.0	34.0	41.0
19	38.266400413005215	40.0	37.0	41.0	34.0	41.0
20	38.175875712864986	40.0	37.0	41.0	34.0	41.0
21	38.07478814482246	40.0	37.0	41.0	34.0	41.0
22	38.104119764097646	40.0	37.0	41.0	34.0	41.0
23	38.02303736788885	40.0	37.0	41.0	34.0	41.0
24	37.96227759029623	40.0	36.0	41.0	33.0	41.0
25	37.84012221469413	40.0	36.0	41.0	33.0	41.0
26	37.78969776285752	40.0	36.0	41.0	33.0	41.0
27	37.72628672534875	40.0	36.0	41.0	33.0	41.0
28	37.62544940732454	40.0	36.0	41.0	33.0	41.0
29	37.562047174909004	40.0	36.0	41.0	33.0	41.0
30	37.46804909719989	39.0	36.0	41.0	33.0	41.0
31	37.38430848968553	39.0	35.0	41.0	33.0	41.0
32	37.35149329747034	39.0	35.0	41.0	33.0	41.0
33	37.242032549185716	39.0	35.0	41.0	32.0	41.0
34	37.09098210156258	39.0	35.0	41.0	32.0	41.0
35	36.95659737830666	39.0	35.0	41.0	31.0	41.0
36	36.82147580777462	39.0	35.0	41.0	31.0	41.0
37	36.78758917474032	39.0	35.0	41.0	31.0	41.0
38	36.71290530075881	39.0	35.0	41.0	31.0	41.0
39	36.65823864768598	39.0	35.0	41.0	31.0	41.0
40	36.53597761096503	39.0	35.0	41.0	31.0	41.0
41	36.41367671961154	39.0	35.0	40.0	30.0	41.0
42	36.34863952040974	39.0	35.0	40.0	30.0	41.0
43	36.218021523355276	38.0	35.0	40.0	30.0	41.0
44	36.134324477881144	38.0	35.0	40.0	30.0	41.0
45	36.11171716928302	38.0	35.0	40.0	30.0	41.0
46	35.895002970560405	38.0	35.0	40.0	30.0	41.0
47	35.79988525573235	38.0	35.0	40.0	29.0	41.0
48	35.7148271282142	38.0	35.0	40.0	29.0	41.0
49	35.74886854551909	38.0	35.0	40.0	29.0	41.0
50	35.584707314066556	37.0	34.0	40.0	29.0	41.0
51	34.556438330517246	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	28.0
10	49.0
11	44.0
12	40.0
13	48.0
14	59.0
15	76.0
16	124.0
17	225.0
18	425.0
19	807.0
20	1353.0
21	2146.0
22	3355.0
23	4932.0
24	7394.0
25	10926.0
26	15213.0
27	19179.0
28	22711.0
29	26866.0
30	33803.0
31	42735.0
32	56739.0
33	80676.0
34	137789.0
35	176266.0
36	194391.0
37	290479.0
38	457326.0
39	571402.0
40	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.80109387063557	4.572855658569997	54.24020850460784	17.38584196618659
2	20.493900850942747	4.685560851999358	53.21321023504038	21.607328062017515
3	21.055434086462306	4.256335728009743	52.603017273739226	22.085212911788723
4	19.316103774048333	4.85591623483091	50.93815951306907	24.889820478051682
5	17.86493172345334	7.97565345377465	49.749379240926814	24.410035581845197
6	22.261407461714064	7.902756550294229	52.26661636950249	17.569219618489214
7	85.10168955836433	2.2699066938172487	8.480509694407653	4.147894053410769
8	87.27515730994206	2.7760605271377607	6.33841588031005	3.6103662826101264
9	81.56996666113645	5.121876393174292	8.291292875011239	5.01686407067802
10	39.81459254199334	28.450600182960567	15.684049156518409	16.05075811852768
11	30.78719387239659	24.30479154636901	24.29047168421043	20.61754289702397
12	26.616082178398603	21.210867153387504	30.543061076760946	21.62998959145294
13	24.685681342748914	21.255309702934692	32.66119576873562	21.39781318558078
14	22.267246628807854	22.959512327593956	32.01485558256814	22.75838546103005
15	21.029713945692038	22.922669963787897	33.48975504230616	22.55786104821391
16	23.578695752515706	20.71875512664968	32.113009200859004	23.589539919975604
17	23.63861672912104	21.028972464156322	30.0058113615362	25.326599445186442
18	24.33463617818172	21.044080150446604	30.22853387782794	24.392749793543736
19	25.260051477355617	23.50172070058883	28.274220262651294	22.964007559404255
20	27.915157828979137	23.10414756965524	27.35334653788368	21.62734806348194
21	27.658558875024212	23.1060939586865	27.418411542643067	21.816935623646216
22	25.467341909185194	21.961061097151692	27.29527926511765	25.27631772854546
23	25.004935486472135	23.4309555565236	26.931026460695456	24.633082496308813
24	25.221911520862044	22.145411943969947	28.574195886445807	24.058480648722195
25	24.66478083196082	23.303142676803958	26.65695634805514	25.375120143180084
26	23.347214485583283	24.60861360563007	27.068200544803556	24.975971363983092
27	22.762834350244365	24.36406372663059	28.157900346735303	24.715201576389745
28	22.185590974686747	25.103876928894703	28.8797789643542	23.83075313206435
29	24.16224172112694	24.0251603222108	27.204216064012098	24.608381892650158
30	24.7158503727335	24.293993721505096	27.784332680520627	23.205823225240774
31	24.72558231788982	25.94124129570191	25.854024530062908	23.479151856345368
32	24.629745829398075	26.21920418640475	25.609428308467443	23.541621675729736
33	25.197118232011427	24.967444326322315	25.7329776693567	24.102459772309555
34	23.515391766403656	24.72053097492773	27.2264141674877	24.53766309118091
35	23.416728379556982	24.529367766500048	27.069915220854913	24.983988633088057
36	23.71244048452111	26.9980378544861	25.403528154517335	23.88599350647545
37	23.451578011735798	27.519206688904934	26.11715779005136	22.91205750930791
38	23.97914212440021	26.200852518395696	25.841882769915497	23.978122587288595
39	24.388439932117365	24.117382088215912	26.79009862631277	24.704079353353954
40	23.89387174779247	23.60826232875252	28.31273095991273	24.18513496354228
41	20.74373378588423	24.32356029774191	28.14863182753881	26.784074088835048
42	21.671002788897425	25.227333604591994	26.149365894259173	26.952297712251404
43	22.68595198350945	24.15807088748852	27.43124844173021	25.72472868727182
44	23.397264489244346	24.368280902864992	27.09317920403811	25.141275403852553
45	24.216925984386254	24.075905464811605	26.384600911466176	25.32256763933597
46	23.55603422308028	24.744721810030576	27.244487779920867	24.454756186968275
47	21.828567615237816	25.415669914664747	28.70205510876144	24.053707361336002
48	21.54768514098808	24.26869066409867	28.872873917552816	25.310750277360437
49	22.482786042722314	24.236389874698887	28.45681009082222	24.82401399175658
50	22.611850172533483	23.688574047590137	28.136443724795416	25.563132055080956
51	22.188603243425607	23.51529908121169	26.960546694336283	27.33555098102641
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	358.0
1	1633.5
2	2909.0
3	7416.0
4	11923.0
5	8255.0
6	4587.0
7	4442.5
8	4298.0
9	4394.0
10	4490.0
11	4657.5
12	4825.0
13	4802.0
14	4779.0
15	4634.5
16	4490.0
17	4252.5
18	4015.0
19	4284.0
20	4553.0
21	4881.5
22	5210.0
23	6326.5
24	7443.0
25	8955.0
26	13168.5
27	15870.0
28	18517.0
29	21164.0
30	23902.0
31	26640.0
32	30563.0
33	34486.0
34	40290.5
35	46095.0
36	51160.5
37	56226.0
38	61103.5
39	65981.0
40	76952.0
41	87923.0
42	97559.5
43	107196.0
44	118352.5
45	129509.0
46	144766.0
47	160023.0
48	177621.5
49	195220.0
50	191089.5
51	186959.0
52	171654.5
53	156350.0
54	145918.5
55	135487.0
56	131678.0
57	127869.0
58	123236.5
59	118604.0
60	113832.0
61	109060.0
62	99782.0
63	90504.0
64	80271.0
65	70038.0
66	59508.0
67	48978.0
68	41093.0
69	33208.0
70	28293.5
71	23379.0
72	19718.0
73	16057.0
74	13058.0
75	7624.5
76	5190.0
77	4069.5
78	2949.0
79	2131.0
80	1313.0
81	1088.5
82	864.0
83	527.0
84	190.0
85	123.5
86	57.0
87	41.5
88	26.0
89	17.0
90	8.0
91	8.0
92	8.0
93	5.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2157842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.155811666315188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34412355888293	18.450309590782098
2	10.452474373903192	5.25880953593789
3	3.8658409206562148	2.917450983958867
4	1.8551362909462588	1.866698366015623
5	1.1176174622417976	1.4057287197569892
6	0.6939938711997379	1.0474787472886562
7	0.5138075947990035	0.9047672961240285
8	0.40941734383252265	0.8239380475499161
9	0.3435968306857913	0.7779111445687097
>10	6.058865549597289	41.351211215528174
>50	1.2539917885144554	19.40387363727487
>100	0.08507144341751682	3.7533114270200834
>500	0.003978824930905275	0.6938183030685254
>1k	0.0018946785385263213	1.0476623012811195
>5k	1.8946785385263215E-4	0.2970306838444082
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6232	0.2888070581627385	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	3668	0.1699846420636914	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3594	0.16655528996098878	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3266	0.15135491847873941	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2694	0.12484695357676791	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.39027787947403E-4	0.0	0.0	0.18296056893878235	0.0
2	1.39027787947403E-4	0.0	0.0	0.6780385218194844	0.0
3	1.39027787947403E-4	0.0	0.0	0.9951609061275107	0.0
4	1.39027787947403E-4	0.0	0.0	1.3707676465654113	0.0
5	1.39027787947403E-4	0.0	0.0	2.017617601288695	0.0
6	1.39027787947403E-4	0.0	0.0	2.5949073194422945	0.0
7	1.39027787947403E-4	0.0	0.0	3.0623187425214637	0.0
8	1.39027787947403E-4	0.0	0.0	3.7889243049305743	0.0
9	1.39027787947403E-4	0.0	0.0	4.218010401132243	0.0
10	1.39027787947403E-4	0.0	0.0	4.910646840686204	0.0
11	1.39027787947403E-4	0.0	0.0	6.077321694544827	0.0
12	1.8537038392987067E-4	0.0	0.0	7.152608949126025	0.0
13	1.8537038392987067E-4	0.0	0.0	7.479926704550194	0.0
14	1.8537038392987067E-4	0.0	0.0	7.603058982075611	0.0
15	1.8537038392987067E-4	0.0	0.0	7.795936866554642	0.0
16	1.8537038392987067E-4	0.0	0.0	8.22428148122059	0.0
17	1.8537038392987067E-4	0.0	0.0	8.81227633904614	0.0
18	2.3171297991233836E-4	0.0	0.0	9.446799163238087	0.0
19	2.3171297991233836E-4	0.0	0.0	9.888397760355021	0.0
20	2.78055575894806E-4	0.0	0.0	10.35673603535384	0.0
21	2.78055575894806E-4	0.0	0.0	11.016654602144179	0.0
22	2.78055575894806E-4	0.0	0.0	11.72731831153532	0.0
23	2.78055575894806E-4	0.0	0.0	12.416015630430772	0.0
24	2.78055575894806E-4	0.0	0.0	12.977131782586492	0.0
25	2.78055575894806E-4	0.0	0.0	13.472858531810948	0.0
26	2.78055575894806E-4	0.0	0.0	13.896290831302755	0.0
27	2.78055575894806E-4	0.0	0.0	14.303827620372576	0.0
28	2.78055575894806E-4	0.0	0.0	14.748114088056493	0.0
29	2.78055575894806E-4	0.0	0.0	15.201112963785114	0.0
30	2.78055575894806E-4	0.0	0.0	15.73868707718174	0.0
31	2.78055575894806E-4	0.0	0.0	16.228574659312407	0.0
32	2.78055575894806E-4	0.0	0.0	16.721798908353808	0.0
33	2.78055575894806E-4	0.0	0.0	17.210527925584913	0.0
34	2.78055575894806E-4	0.0	0.0	17.67738323751229	0.0
35	2.78055575894806E-4	0.0	0.0	18.17704910739526	0.0
36	2.78055575894806E-4	0.0	0.0	18.649650901224465	0.0
37	2.78055575894806E-4	0.0	0.0	19.124245426680915	0.0
38	2.78055575894806E-4	0.0	0.0	19.616867221974548	0.0
39	2.78055575894806E-4	0.0	0.0	20.105086470649844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATA	20	7.034665E-4	45.0	28
TACGGGA	380	0.0	40.855263	4
CGTATGG	170	0.0	39.705883	2
TTTGGGC	5655	0.0	38.236076	4
CGTTTTT	3645	0.0	37.901234	1
TTTGGGA	11380	0.0	37.76362	4
TTACGGG	520	0.0	37.644234	3
GCAACGA	60	1.5643309E-10	37.500004	9
CGTTTGG	1555	0.0	37.475887	2
TATGGGA	1040	0.0	37.21154	4
TCGATTG	85	0.0	37.058823	1
ACACGCG	415	0.0	36.3253	36
TTGGGAT	5210	0.0	36.233204	5
TTGGGCA	2795	0.0	36.2254	5
TGGGCGA	4325	0.0	36.10405	6
GGGCGAT	6360	0.0	36.084908	7
TTGGGAC	5045	0.0	36.080276	5
ATCTCGA	25	0.0021075173	36.0	45
CGAAACG	25	0.0021075173	36.0	19
TCGAACG	25	0.0021075173	36.0	34
>>END_MODULE