##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933151.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509081
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08081425156311	33.0	31.0	34.0	30.0	34.0
2	32.27087830816707	34.0	31.0	34.0	30.0	34.0
3	32.34472706700898	34.0	31.0	34.0	30.0	34.0
4	35.855655583296176	37.0	35.0	37.0	35.0	37.0
5	35.12933305308978	37.0	35.0	37.0	35.0	37.0
6	34.83793148830932	37.0	35.0	37.0	32.0	37.0
7	35.6405463963495	37.0	35.0	37.0	35.0	37.0
8	35.90326883148261	37.0	35.0	37.0	35.0	37.0
9	37.76733957857394	39.0	38.0	39.0	35.0	39.0
10	37.14909611633512	39.0	37.0	39.0	34.0	39.0
11	37.084196817402336	39.0	37.0	39.0	33.0	39.0
12	36.898444451865224	39.0	35.0	39.0	33.0	39.0
13	36.746272204226834	39.0	35.0	39.0	33.0	39.0
14	37.80552210748388	40.0	37.0	41.0	33.0	41.0
15	37.92894254548883	40.0	37.0	41.0	33.0	41.0
16	37.9985365786584	40.0	36.0	41.0	33.0	41.0
17	37.978584940314015	40.0	36.0	41.0	33.0	41.0
18	37.9489216843685	39.0	36.0	41.0	33.0	41.0
19	37.96258355742996	40.0	36.0	41.0	34.0	41.0
20	37.84764703455835	39.0	36.0	41.0	33.0	41.0
21	37.7275148748431	39.0	36.0	41.0	33.0	41.0
22	37.78071858898682	39.0	36.0	41.0	33.0	41.0
23	37.69243401344776	39.0	36.0	41.0	33.0	41.0
24	37.65804459408228	39.0	36.0	41.0	33.0	41.0
25	37.55476240519682	39.0	35.0	41.0	33.0	41.0
26	37.53052657632086	39.0	35.0	41.0	33.0	41.0
27	37.520594954437506	39.0	35.0	41.0	33.0	41.0
28	37.46052396377001	39.0	35.0	41.0	33.0	41.0
29	37.42635454868675	39.0	35.0	41.0	33.0	41.0
30	37.38991830376698	39.0	36.0	41.0	33.0	41.0
31	37.29956333078626	39.0	35.0	41.0	32.0	41.0
32	37.251588646993305	39.0	35.0	41.0	32.0	41.0
33	37.15439586234803	39.0	35.0	41.0	32.0	41.0
34	37.014530104246674	39.0	35.0	41.0	32.0	41.0
35	36.91404707698775	39.0	35.0	41.0	31.0	41.0
36	36.78150628289015	39.0	35.0	41.0	31.0	41.0
37	36.694622270326335	39.0	35.0	40.0	31.0	41.0
38	36.615212510386364	39.0	35.0	40.0	31.0	41.0
39	36.62924956932198	39.0	35.0	40.0	31.0	41.0
40	36.57703783877222	39.0	35.0	40.0	31.0	41.0
41	36.523378401472456	39.0	35.0	40.0	31.0	41.0
42	36.423109485523916	39.0	35.0	40.0	30.0	41.0
43	36.2153861566234	38.0	35.0	40.0	30.0	41.0
44	36.109155517491324	38.0	35.0	40.0	30.0	41.0
45	36.199445667781745	38.0	35.0	40.0	30.0	41.0
46	36.070861022116326	38.0	35.0	40.0	30.0	41.0
47	36.01572834185522	38.0	35.0	40.0	30.0	41.0
48	35.939726683965816	38.0	35.0	40.0	29.0	41.0
49	36.021803995827774	38.0	35.0	40.0	30.0	41.0
50	35.81592320279091	38.0	35.0	40.0	29.0	41.0
51	34.7390945645192	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	3.0
12	8.0
13	10.0
14	5.0
15	6.0
16	19.0
17	41.0
18	67.0
19	178.0
20	249.0
21	432.0
22	751.0
23	1153.0
24	1764.0
25	2596.0
26	3843.0
27	4647.0
28	5280.0
29	6523.0
30	8517.0
31	11005.0
32	14597.0
33	20893.0
34	35165.0
35	48253.0
36	45208.0
37	65202.0
38	108013.0
39	124597.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.040500431169107	4.218582111687532	56.54404701805803	17.19687043908533
2	19.308322251272394	4.60378603797824	55.15743074284839	20.93046096790098
3	19.880922682245064	4.394781969863342	54.049159171133866	21.675136176757725
4	17.51784097226178	4.401460671288066	53.95880026950525	24.121898086944906
5	17.674594023347954	7.184318409054748	52.023548315494	23.1175392521033
6	20.37495015527981	6.97001066627904	54.33123609013104	18.32380308831011
7	82.41006833883017	1.634317525108971	10.835407332035569	5.120206804025293
8	84.6953628204549	2.235007788544456	7.781865754172715	5.287763636827931
9	79.09095016313711	4.56842820690617	10.631903370976328	5.708718258980397
10	36.16477535009164	27.100795354766728	19.402413368403064	17.332015926738574
11	28.609199714780164	25.85777116018865	26.10095446500655	19.432074660024632
12	26.384406410767635	22.045214808645383	32.0338020865049	19.53657669408208
13	22.00160681698983	28.212406277193608	31.704777825139814	18.08120908067675
14	18.976155071589787	29.505127867667426	30.724973039653808	20.79374402108898
15	15.991954129107155	25.21563366144091	38.25638749039937	20.536024719052566
16	17.465197090443368	23.117342819708455	34.833553010228236	24.583907079619944
17	17.576181393530696	22.98160803487068	30.506933081376047	28.935277490222578
18	19.34859089221558	22.963536254544955	34.67542493237815	23.012447920861316
19	21.78553118266052	25.074595201942323	30.842832476560705	22.297041138836452
20	24.36488495936796	25.059077042749582	30.10267521278539	20.47336278509707
21	21.967624012681675	27.107670488586294	29.496091977504562	21.428613521227465
22	20.287341307179016	25.324850072974638	28.4064814833003	25.98132713654605
23	18.611969411547474	26.687108731223518	28.077850086724904	26.623071770504104
24	20.489273809079496	24.479012180772806	32.09901764159338	22.932696368554318
25	17.954903050791522	26.713823536922416	30.062603004237047	25.268670408049015
26	17.888312468939127	27.37678286952371	30.77349184118048	23.96141282035668
27	19.71886595649808	25.68157130201284	30.892529872456446	23.70703286903263
28	17.489947572193813	25.261795274229442	33.89539189244933	23.352865261127402
29	17.85040101673408	24.34249166635565	32.671618072566055	25.13548924434422
30	21.047534675228498	25.155721781013234	30.581970256206777	23.21477328755149
31	23.07039547734054	27.2500839748488	27.117099243538846	22.562421304271815
32	22.8401767105824	27.989651941439575	26.43194305032009	22.738228297657937
33	24.117380141863475	25.649749254047983	26.461211477151963	23.77165912693658
34	20.46688051606719	24.065325557229595	29.862438393890166	25.60535553281305
35	21.2070377798425	23.980859627446318	29.006975314340938	25.80512727837024
36	25.91984379695962	23.358561800577903	28.75672044330863	21.964873959153845
37	23.662835580192542	25.893521856050413	29.772668789446083	20.670973774310962
38	21.891408243481884	28.02775982603947	27.559268564334555	22.52156336614409
39	20.878995680451638	25.960308870297656	29.220300895142426	23.940394554108284
40	22.40959690108254	23.024626729341698	30.37768056556815	24.188095804007613
41	21.62484948367745	22.789890017502128	30.066531652133943	25.51872884668648
42	25.766430096585808	22.739799756816694	27.47892771484302	24.014842431754474
43	25.819073978404223	23.45854588955392	28.209852656060626	22.51252747598123
44	21.72286924870502	24.856555243664562	28.250121297003815	25.1704542106266
45	21.137697144462276	24.737910077178288	27.204118794455106	26.92027398390433
46	22.834087306342212	24.171006185656115	29.82727699521294	23.167629512788732
47	21.698118766954572	23.15368281275475	31.954443398987586	23.19375502130309
48	22.23595066403971	22.985929547557266	30.310893551320913	24.467226237082116
49	22.79617585413716	20.72499268289329	32.5407940975994	23.938037365370146
50	22.547492442263607	21.295039492732982	30.20187357218203	25.955594492821376
51	21.212537886898154	21.159304707895206	28.034045662674504	29.59411174253213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	223.5
2	360.0
3	1311.0
4	2262.0
5	1567.5
6	873.0
7	893.5
8	914.0
9	1028.5
10	1143.0
11	1137.5
12	1132.0
13	1100.5
14	1069.0
15	1148.5
16	1228.0
17	1165.0
18	1102.0
19	1197.0
20	1292.0
21	1208.0
22	1124.0
23	1376.5
24	1629.0
25	1871.0
26	2869.0
27	3625.0
28	3963.0
29	4301.0
30	5607.5
31	6914.0
32	8355.0
33	9796.0
34	11587.0
35	13378.0
36	15002.5
37	16627.0
38	18377.5
39	20128.0
40	23644.0
41	27160.0
42	30321.5
43	33483.0
44	35635.5
45	37788.0
46	40639.0
47	43490.0
48	50220.5
49	56951.0
50	61887.0
51	66823.0
52	60721.0
53	54619.0
54	43861.5
55	33104.0
56	27131.5
57	21159.0
58	17569.0
59	13979.0
60	12155.0
61	10331.0
62	8523.5
63	6716.0
64	5682.5
65	4649.0
66	3863.5
67	3078.0
68	2391.0
69	1704.0
70	1344.5
71	985.0
72	815.0
73	645.0
74	631.5
75	467.0
76	316.0
77	242.5
78	169.0
79	158.5
80	148.0
81	95.5
82	43.0
83	26.5
84	10.0
85	5.5
86	1.0
87	3.5
88	6.0
89	4.0
90	2.0
91	4.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.15809977264408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.03143088784516	22.842358414849972
2	12.387805282706989	7.967365564907575
3	5.020955911664624	4.843932034840742
4	2.4851268206691195	3.1966782498700526
5	1.41849645077342	2.2808075195556574
6	0.9599686295391353	1.8522460180396747
7	0.6654273923734396	1.4979216332776815
8	0.4673627466748086	1.202359827006838
9	0.3563252328852808	1.0312868151571948
>10	4.6723177982111235	37.40094734565657
>50	0.45043995198417697	8.866492999917327
>100	0.07884227110713546	4.151009313669399
>500	0.0024447215847173786	0.5406925483856305
>1k	0.0030559019808967234	2.325901714865704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2888	0.5672967563118639	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	2835	0.5568858393850881	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2689	0.5282067097377431	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	2033	0.3993470587195358	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1389	0.27284459643946646	No Hit
TCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	889	0.1746283990170523	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT	664	0.13043111017696593	No Hit
GCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	624	0.1225738143831728	No Hit
TGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	574	0.1127521946409314	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5873328605860364	0.0
2	0.0	0.0	0.0	2.2383471392568177	0.0
3	0.0	0.0	0.0	3.1165963766080447	0.0
4	0.0	0.0	0.0	4.105044187467221	0.0
5	0.0	0.0	0.0	5.625823788355881	0.0
6	0.0	0.0	0.0	6.6042535470779695	0.0
7	0.0	0.0	0.0	7.528075100033197	0.0
8	0.0	0.0	0.0	9.0700693995651	0.0
9	0.0	0.0	0.0	9.939675611543153	0.0
10	0.0	0.0	0.0	11.480884181495675	0.0
11	0.0	0.0	0.0	13.97537916363015	0.0
12	0.0	0.0	0.0	16.30251374535683	0.0
13	0.0	0.0	0.0	16.973330373751917	0.0
14	0.0	0.0	0.0	17.186263089763713	0.0
15	0.0	0.0	0.0	17.51685881028756	0.0
16	0.0	0.0	0.0	18.44048393084794	0.0
17	0.0	0.0	0.0	19.934941590827393	0.0
18	0.0	0.0	0.0	21.626224510441364	0.0
19	0.0	0.0	0.0	22.596207676185127	0.0
20	0.0	0.0	0.0	23.56049430247839	0.0
21	0.0	0.0	0.0	24.808429306927582	0.0
22	0.0	0.0	0.0	26.089168521315862	0.0
23	0.0	0.0	0.0	27.427462427393674	0.0
24	0.0	0.0	0.0	28.323979877465472	0.0
25	0.0	0.0	0.0	29.14369226115294	0.0
26	0.0	0.0	0.0	29.84475947835413	0.0
27	0.0	0.0	0.0	30.517343998302824	0.0
28	0.0	0.0	0.0	31.25200115502248	0.0
29	0.0	0.0	0.0	32.044998733011056	0.0
30	0.0	0.0	0.0	32.97274893386318	0.0
31	0.0	0.0	0.0	33.7409960301013	0.0
32	3.928647896896565E-4	0.0	0.0	34.47211740371375	0.0
33	3.928647896896565E-4	0.0	0.0	35.20873888438186	0.0
34	3.928647896896565E-4	0.0	0.0	35.96971798201072	0.0
35	3.928647896896565E-4	0.0	0.0	36.8214488460579	0.0
36	3.928647896896565E-4	0.0	0.0	37.524087522417844	0.0
37	3.928647896896565E-4	0.0	0.0	38.19097550291604	0.0
38	3.928647896896565E-4	0.0	0.0	38.85805991580907	0.0
39	3.928647896896565E-4	0.0	0.0	39.51767989769801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	7.029578E-4	45.000004	36
ACGTTTG	20	7.029578E-4	45.000004	1
GCGGTCC	20	7.029578E-4	45.000004	20
GCAACCC	20	7.029578E-4	45.000004	22
ATTCGCG	20	7.029578E-4	45.000004	12
TAACGCC	20	7.029578E-4	45.000004	12
CGACAAT	20	7.029578E-4	45.000004	20
ATCCGGA	20	7.029578E-4	45.000004	20
AACGGCG	20	7.029578E-4	45.000004	10
GCCGATA	35	1.2101373E-7	45.000004	9
CGGGCTT	40	6.8030204E-9	45.000004	6
AGACCCG	20	7.029578E-4	45.000004	39
GTCGTTG	20	7.029578E-4	45.000004	1
CGTGTTG	20	7.029578E-4	45.000004	1
TAAGGCG	20	7.029578E-4	45.000004	18
TAGGATA	20	7.029578E-4	45.000004	23
CGTCCGT	20	7.029578E-4	45.000004	44
CGATAGT	20	7.029578E-4	45.000004	11
TACACGA	20	7.029578E-4	45.000004	27
GCATAGG	35	1.2101373E-7	45.000004	2
>>END_MODULE