##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391023
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.956301803218736	33.0	31.0	34.0	30.0	34.0
2	32.144193564061446	33.0	31.0	34.0	30.0	34.0
3	32.230628377358876	34.0	31.0	34.0	30.0	34.0
4	35.757666428829	37.0	35.0	37.0	35.0	37.0
5	34.98369916859111	37.0	35.0	37.0	33.0	37.0
6	34.71827232669178	37.0	35.0	37.0	32.0	37.0
7	35.57429102635906	37.0	35.0	37.0	33.0	37.0
8	35.823887597404756	37.0	35.0	37.0	35.0	37.0
9	37.63339496653649	39.0	37.0	39.0	35.0	39.0
10	37.08729921257829	39.0	37.0	39.0	33.0	39.0
11	36.97486592860267	39.0	37.0	39.0	33.0	39.0
12	36.68201614738775	39.0	35.0	39.0	33.0	39.0
13	36.41836157975362	39.0	35.0	39.0	32.0	39.0
14	37.36389675287643	39.0	36.0	41.0	32.0	41.0
15	37.5365822470801	39.0	36.0	41.0	33.0	41.0
16	37.64873421768029	39.0	36.0	41.0	33.0	41.0
17	37.65312526373129	39.0	35.0	41.0	33.0	41.0
18	37.64775985044358	39.0	36.0	41.0	33.0	41.0
19	37.659771931574355	39.0	36.0	41.0	33.0	41.0
20	37.543055012109264	39.0	35.0	41.0	33.0	41.0
21	37.396089232602684	39.0	35.0	41.0	33.0	41.0
22	37.44302253320137	39.0	35.0	41.0	33.0	41.0
23	37.39431184354884	39.0	35.0	41.0	33.0	41.0
24	37.346181682407426	39.0	35.0	41.0	33.0	41.0
25	37.266759244341124	39.0	35.0	41.0	32.0	41.0
26	37.241446155341244	39.0	35.0	41.0	33.0	41.0
27	37.208880296043965	39.0	35.0	41.0	32.0	41.0
28	37.12012848349074	39.0	35.0	40.0	32.0	41.0
29	37.089800344225274	39.0	35.0	40.0	32.0	41.0
30	37.04987430406907	39.0	35.0	40.0	32.0	41.0
31	36.933075547985666	39.0	35.0	40.0	32.0	41.0
32	36.916616158128804	39.0	35.0	40.0	32.0	41.0
33	36.83945445664322	39.0	35.0	40.0	31.0	41.0
34	36.784089426964655	39.0	35.0	40.0	31.0	41.0
35	36.6691498965534	39.0	35.0	40.0	31.0	41.0
36	36.545029320525906	38.0	35.0	40.0	31.0	41.0
37	36.44965385667851	38.0	35.0	40.0	31.0	41.0
38	36.326883584853064	38.0	35.0	40.0	30.0	41.0
39	36.32290939407656	38.0	35.0	40.0	30.0	41.0
40	36.237032092741345	38.0	35.0	40.0	30.0	41.0
41	36.17908665219181	38.0	35.0	40.0	30.0	41.0
42	36.11386031000734	38.0	35.0	40.0	30.0	41.0
43	35.96116852461364	38.0	35.0	40.0	30.0	41.0
44	35.85269664444291	38.0	35.0	40.0	30.0	41.0
45	35.86330727348519	38.0	35.0	40.0	30.0	41.0
46	35.56935525531746	38.0	34.0	40.0	29.0	41.0
47	35.490198786260656	38.0	34.0	40.0	29.0	41.0
48	35.43775941568654	37.0	34.0	40.0	29.0	41.0
49	35.58095303856806	38.0	34.0	40.0	29.0	41.0
50	35.338136119870185	37.0	34.0	40.0	28.0	41.0
51	34.06469951895413	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	1.0
11	5.0
12	4.0
13	7.0
14	6.0
15	4.0
16	8.0
17	34.0
18	70.0
19	109.0
20	220.0
21	384.0
22	639.0
23	979.0
24	1423.0
25	2217.0
26	2980.0
27	3961.0
28	4666.0
29	5862.0
30	7395.0
31	9734.0
32	12944.0
33	18296.0
34	32071.0
35	42182.0
36	34274.0
37	49652.0
38	80079.0
39	80787.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.40771258979651	4.093365351910245	53.69760858056944	16.801313477723816
2	20.735353163368906	4.225326898929219	52.567240290213114	22.472079647488766
3	21.311278364699774	5.011214174102291	51.47318699923022	22.20432046196771
4	18.901957173874685	5.051876743823254	50.939458804213565	25.106707278088503
5	17.204103083450335	8.307183976390135	49.61396132708306	24.87475161307647
6	20.787524007539197	7.244075156704337	54.741792682271885	17.226608153484577
7	81.18601719080463	1.7178017661365188	11.531290998227725	5.564890044831122
8	81.4719338760124	2.6402538981082952	8.938348895077782	6.949463330801513
9	74.05370016597489	6.60140196356736	12.201839789475299	7.143058080982449
10	38.77495697184053	27.569989489109336	19.038777770105593	14.616275768944536
11	31.25545044664892	23.816757582034818	28.050012403362462	16.877779567953805
12	29.78213557770259	19.558440296350852	32.64974183104319	18.009682294903367
13	24.27043933477059	27.08919935655959	31.746981635351375	16.89337967331845
14	17.701516279093557	29.798758640796063	31.185122102791908	21.31460297731847
15	13.9715566603499	24.542801830071376	42.48548039373643	19.00016111584229
16	16.516164010812666	22.197415497298113	37.89521332504738	23.39120716684185
17	16.412333801336494	21.14351329717178	31.430887697143135	31.013265204348595
18	17.84958941034159	21.75805515276595	35.4723379443153	24.920017492577163
19	22.656979256974655	24.0085621561903	31.93571733632037	21.398741250514675
20	25.185219283776146	24.753275382777996	30.071121136096853	19.990384197349005
21	21.69207437925646	26.941381964743762	30.122013283106106	21.244530372893667
22	19.503200578994072	25.631740332410114	28.61391785137959	26.251141237216224
23	18.99632502435918	27.211442805154682	27.281003930715077	26.51122823977106
24	20.002915429527164	22.987138863954296	30.96493045166141	26.04501525485713
25	17.888461804037103	25.04865442697744	30.11613127616534	26.946752492820114
26	16.385481160954726	26.763898798791885	29.96013022251888	26.890489817734508
27	17.478255754776573	27.730593852535527	31.984563567871966	22.80658682481593
28	15.065354211900578	27.040097385575784	35.08668288054667	22.807865521976968
29	16.48317362405792	25.649130613800214	32.0873708196193	25.780324942522565
30	20.225152996115316	25.60923526237587	31.58586579306077	22.579745948448046
31	22.58000168788025	29.328965303831232	25.252478754446667	22.838554253841846
32	20.990069637847387	30.26957493548973	24.619012180869156	24.121343245793724
33	20.744048304063956	26.83755175526759	27.255941466358756	25.162458474309695
34	18.99274467230828	25.41768642765259	29.018497633131556	26.571071266907577
35	16.441999575472543	25.907938919194013	28.23235461852628	29.41770688680717
36	20.672185523613702	24.432578134790024	30.916851438406432	23.978384903189838
37	18.951826363155106	29.351726113297683	31.199699250427727	20.496748273119483
38	17.272896990714102	32.67506003483171	27.356702802648435	22.69534017180575
39	19.89473764970347	26.449083557744686	29.809755436380982	23.846423356170867
40	23.29300322487424	23.430591039401776	29.514887871045946	23.761517864678037
41	18.12092894791355	24.277344299440188	27.162340834170877	30.43938591847538
42	19.626722724750206	24.65737309570025	26.646002920544316	29.069901259005228
43	20.51925334315373	23.757937512627134	28.739997391457788	26.98281175276135
44	18.346746866552607	25.7046260705892	29.45683501993489	26.491792042923308
45	19.5356794868844	25.22306871974283	27.293279423461026	27.947972369911746
46	21.921472649946423	24.956843970815015	30.8352705595323	22.286412819706257
47	18.801707316449416	24.97653590709498	31.574868997475853	24.646887778979753
48	17.42148160082655	24.583720139224546	31.51835058295804	26.476447676990865
49	19.73208737081962	20.741235170309675	33.1924209061871	26.3342565526836
50	20.683182319198615	21.558322656212038	29.803873429440213	27.95462159514913
51	18.13448313782054	22.1130214846697	27.53341875030369	32.21907662720607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	141.5
2	255.0
3	922.5
4	1590.0
5	1099.0
6	608.0
7	657.5
8	707.0
9	738.0
10	769.0
11	867.5
12	966.0
13	934.0
14	902.0
15	832.5
16	763.0
17	796.0
18	829.0
19	741.5
20	654.0
21	785.0
22	916.0
23	1160.5
24	1405.0
25	1861.0
26	2505.5
27	2694.0
28	3151.0
29	3608.0
30	3904.0
31	4200.0
32	4630.0
33	5060.0
34	6454.0
35	7848.0
36	8417.5
37	8987.0
38	10799.0
39	12611.0
40	17536.0
41	22461.0
42	29162.5
43	35864.0
44	42986.0
45	50108.0
46	54100.5
47	58093.0
48	53975.0
49	49857.0
50	40888.5
51	31920.0
52	27650.0
53	23380.0
54	19000.0
55	14620.0
56	13194.0
57	11768.0
58	10975.0
59	10182.0
60	9119.5
61	8057.0
62	7152.0
63	6247.0
64	5113.0
65	3979.0
66	3300.0
67	2621.0
68	2127.5
69	1634.0
70	1268.0
71	902.0
72	751.0
73	600.0
74	512.5
75	348.5
76	272.0
77	217.0
78	162.0
79	117.0
80	72.0
81	60.5
82	49.0
83	38.0
84	27.0
85	14.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.07493425674086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34054154225036	23.595837213502215
2	11.872496076835816	7.853640544095168
3	5.065683598009909	5.026414559088847
4	2.7688183813699805	3.66313943730671
5	1.6696162234438316	2.7611223412196346
6	1.0458253396094912	2.0754362610970563
7	0.6857933289310499	1.5877798487674157
8	0.5571597517726908	1.4742417728307047
9	0.4032271454277781	1.2003040193001342
>10	3.947536785218909	31.712912478158184
>50	0.5578873500829884	11.501376976701593
>100	0.07848897890822733	4.241944015726609
>500	0.0038474989660895746	0.8666118301763426
>1k	0.0030779991728716602	2.4392387020293884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2652	0.678220974213793	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2481	0.6344895313063426	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2424	0.6199123836705258	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2027	0.5183838290842226	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	868	0.22198182715594733	No Hit
TCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	843	0.21558834135076452	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	625	0.1598371451295704	No Hit
TGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	558	0.14270260317168043	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	511	0.13068284985793674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7434345294266578	0.0
2	0.0	0.0	0.0	2.7497103750930254	0.0
3	0.0	0.0	0.0	3.809750321592336	0.0
4	0.0	0.0	0.0	5.063129278840375	0.0
5	0.0	0.0	0.0	6.94690593647944	0.0
6	0.0	0.0	0.0	8.229950667863527	0.0
7	0.0	0.0	0.0	9.415558675576628	0.0
8	0.0	0.0	0.0	11.590622546499823	0.0
9	0.0	0.0	0.0	12.983635233733054	0.0
10	0.0	0.0	0.0	15.130823506545651	0.0
11	0.0	0.0	0.0	18.302248205348533	0.0
12	0.0	0.0	0.0	21.357822941361505	0.0
13	0.0	0.0	0.0	22.277206200146793	0.0
14	0.0	0.0	0.0	22.577955772422595	0.0
15	0.0	0.0	0.0	23.068975482260633	0.0
16	0.0	0.0	0.0	24.210084828769663	0.0
17	2.5573943220731264E-4	0.0	0.0	26.013303565263424	0.0
18	2.5573943220731264E-4	0.0	0.0	28.285036941560982	0.0
19	2.5573943220731264E-4	0.0	0.0	29.417962626239376	0.0
20	5.114788644146253E-4	0.0	0.0	30.673387498945075	0.0
21	5.114788644146253E-4	0.0	0.0	32.08430194643282	0.0
22	5.114788644146253E-4	0.0	0.0	33.38320252261376	0.0
23	5.114788644146253E-4	0.0	0.0	34.6821030987947	0.0
24	7.672182966219378E-4	0.0	0.0	35.60634540679193	0.0
25	7.672182966219378E-4	0.0	0.0	36.3991376466346	0.0
26	7.672182966219378E-4	0.0	0.0	37.11137196533196	0.0
27	7.672182966219378E-4	0.0	0.0	37.76836656667255	0.0
28	7.672182966219378E-4	0.0	0.0	38.45758433647126	0.0
29	7.672182966219378E-4	0.0	0.0	39.25114379461055	0.0
30	7.672182966219378E-4	0.0	0.0	40.175386102607774	0.0
31	7.672182966219378E-4	0.0	0.0	40.93109612478038	0.0
32	7.672182966219378E-4	0.0	0.0	41.65074688701176	0.0
33	7.672182966219378E-4	0.0	0.0	42.34917127636993	0.0
34	7.672182966219378E-4	0.0	0.0	43.06779908087248	0.0
35	7.672182966219378E-4	0.0	0.0	43.84422399705388	0.0
36	7.672182966219378E-4	0.0	0.0	44.504031732148746	0.0
37	7.672182966219378E-4	0.0	0.0	45.176370699421774	0.0
38	7.672182966219378E-4	0.0	0.0	45.82262424460965	0.0
39	7.672182966219378E-4	0.0	0.0	46.532301168984944	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	25	3.8860686E-5	45.0	3
TCCGGAC	20	7.027571E-4	45.0	17
TATTTCG	20	7.027571E-4	45.0	27
TTGATAA	20	7.027571E-4	45.0	20
CTATGAC	20	7.027571E-4	45.0	28
TGCCCGC	20	7.027571E-4	45.0	44
CAACGCC	25	3.8860686E-5	45.0	11
GGCACGC	20	7.027571E-4	45.0	8
GCAACGC	25	3.8860686E-5	45.0	10
TTTCGCA	20	7.027571E-4	45.0	13
GACGTGA	20	7.027571E-4	45.0	39
CAGTTAG	20	7.027571E-4	45.0	21
CGGTTCT	20	7.027571E-4	45.0	13
TGGGTCG	50	2.1827873E-11	45.0	6
ATCAGGC	20	7.027571E-4	45.0	16
TTATGCG	25	3.8860686E-5	45.0	1
TTCACGG	20	7.027571E-4	45.0	2
GTGATCG	25	3.8860686E-5	45.0	13
ATTCGGT	20	7.027571E-4	45.0	11
CCCACGT	20	7.027571E-4	45.0	35
>>END_MODULE