FastQCFastQC Report
Sat 14 Jan 2017
SRR2933149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726690
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC51540.29849017484319706Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT49130.2845328344983755Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.27457157914854435No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC42630.24688855556005998Illumina PCR Primer Index 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC41550.24063381382877064Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC41490.24028632817703235No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC38120.220769217404398Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG36320.2103446478522491No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG23230.13453486149801064No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC21710.12573189165397378No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGG207.034271E-445.00000413
TATACCG207.034271E-445.00000439
TACGGGA2600.042.4038434
TATGGGA8400.039.6428574
CGTTTTT26750.039.3644831
TGGGCGA26200.038.3874056
CGTTGAT2000.038.25000425
ACATACG2650.038.20754617
TACGGGT1250.037.84
TTACGGG5200.037.2115363
TTTGGGC42100.037.1437074
TTTGGGA72800.037.0570034
TTGGGAC31050.036.8840565
TCGTTGA2150.036.62790724
TTAATCG2150.036.62790720
ATACGAA2650.036.50943419
TTGGGAT33400.036.3772475
TTTACGG3100.036.290322
GTCGAAC250.00210740136.00000421
TAAACCG250.00210740136.00000411
Produced by FastQC (version 0.11.5)