##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1726690
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09168408921115	33.0	31.0	34.0	30.0	34.0
2	32.27206215360024	34.0	31.0	34.0	30.0	34.0
3	32.35788937215134	34.0	31.0	34.0	30.0	34.0
4	35.87235809554697	37.0	35.0	37.0	35.0	37.0
5	35.07728138808935	37.0	35.0	37.0	33.0	37.0
6	34.73567056043644	37.0	35.0	37.0	32.0	37.0
7	35.55794323242736	37.0	35.0	37.0	33.0	37.0
8	35.82349118834301	37.0	35.0	37.0	35.0	37.0
9	37.69951178265931	39.0	37.0	39.0	35.0	39.0
10	37.18467298704458	39.0	37.0	39.0	34.0	39.0
11	37.08475290874448	39.0	37.0	39.0	33.0	39.0
12	36.820426944037436	39.0	35.0	39.0	33.0	39.0
13	36.656866026906975	39.0	35.0	39.0	33.0	39.0
14	37.49425722046227	40.0	36.0	41.0	32.0	41.0
15	37.64973040904852	40.0	36.0	41.0	32.0	41.0
16	37.766592150299125	40.0	36.0	41.0	33.0	41.0
17	37.75590233336615	40.0	36.0	41.0	33.0	41.0
18	37.734621153768195	39.0	36.0	41.0	33.0	41.0
19	37.73822052597745	40.0	36.0	41.0	33.0	41.0
20	37.59660332775426	39.0	36.0	41.0	33.0	41.0
21	37.468827062182555	39.0	35.0	41.0	32.0	41.0
22	37.587326039995595	39.0	35.0	41.0	33.0	41.0
23	37.53965506257637	39.0	35.0	41.0	33.0	41.0
24	37.466299683208916	39.0	35.0	41.0	33.0	41.0
25	37.348178306470764	39.0	35.0	41.0	32.0	41.0
26	37.3290353219165	39.0	35.0	41.0	32.0	41.0
27	37.27656267193301	39.0	35.0	41.0	32.0	41.0
28	37.17749451262242	39.0	35.0	41.0	32.0	41.0
29	37.10776688345911	39.0	35.0	41.0	32.0	41.0
30	36.985300777788716	39.0	35.0	41.0	31.0	41.0
31	36.8480462619231	39.0	35.0	41.0	31.0	41.0
32	36.81501427586886	39.0	35.0	41.0	31.0	41.0
33	36.71924028053675	39.0	35.0	41.0	31.0	41.0
34	36.51742466800642	39.0	35.0	40.0	30.0	41.0
35	36.40820413623754	39.0	35.0	40.0	30.0	41.0
36	36.301370830896104	38.0	35.0	40.0	30.0	41.0
37	36.27029113506188	38.0	35.0	40.0	30.0	41.0
38	36.13199647881206	38.0	35.0	40.0	30.0	41.0
39	36.065490620783116	38.0	35.0	40.0	30.0	41.0
40	35.91720691033133	38.0	35.0	40.0	29.0	41.0
41	35.7799292287556	38.0	35.0	40.0	29.0	41.0
42	35.76653076116732	38.0	35.0	40.0	29.0	41.0
43	35.691127533025615	38.0	34.0	40.0	29.0	41.0
44	35.64602679114375	38.0	34.0	40.0	29.0	41.0
45	35.638307397390385	38.0	34.0	40.0	29.0	41.0
46	35.40249089298021	37.0	34.0	40.0	28.0	41.0
47	35.29709617823698	37.0	34.0	40.0	28.0	41.0
48	35.21977309186941	37.0	34.0	40.0	28.0	41.0
49	35.289830832401876	37.0	34.0	40.0	28.0	41.0
50	35.068481313959076	37.0	34.0	40.0	27.0	41.0
51	33.955590754565094	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	26.0
10	39.0
11	33.0
12	38.0
13	24.0
14	37.0
15	66.0
16	123.0
17	272.0
18	460.0
19	841.0
20	1564.0
21	2591.0
22	3799.0
23	5545.0
24	7946.0
25	11063.0
26	15132.0
27	18629.0
28	22060.0
29	26968.0
30	34200.0
31	43993.0
32	58166.0
33	83219.0
34	136597.0
35	145118.0
36	158937.0
37	230129.0
38	344544.0
39	374369.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.86799599233215	4.502603246674273	52.152731526794035	16.476669234199537
2	21.169636703751106	5.775443188991654	50.9213002913088	22.133619815948432
3	21.94904702060011	5.824728237263202	49.97944043227215	22.24678430986454
4	19.23906433696842	5.016939925522243	50.73145729690911	25.012538440600224
5	19.193775373691864	7.867306812456203	48.40596748692585	24.532950326926084
6	22.23879213987456	8.327899043835316	51.45220045288963	17.981108363400494
7	82.47531404015776	2.399330510977651	10.59576414990531	4.52959129895928
8	84.28803085672587	3.6018046088180276	7.670745762122906	4.439418772333193
9	79.04510942902317	4.888312320103783	9.668962002443983	6.397616248429075
10	42.23172659828921	25.425930537618218	16.901470443449607	15.440872420642965
11	33.350746225437106	22.56861393764949	24.12755039989807	19.953089437015333
12	28.65331935668823	20.03689139335955	30.509124394071897	20.800664855880328
13	26.76085458304617	21.474207877499722	32.3697942305799	19.395143308874204
14	22.31216952666663	23.02387805570195	33.88830652867625	20.775645888955168
15	19.91260735858782	20.631091857832036	36.27721247010175	23.17908831347839
16	23.9117038958933	21.186142272208677	30.831069850407427	24.0710839814906
17	24.901400946319256	20.771881461061337	28.98603686822765	25.340680724391756
18	25.810770896918385	19.538017826013935	31.172184931863857	23.47902634520383
19	26.132600524703335	22.4300250768812	27.801863681378826	23.63551071703664
20	26.52873416768499	25.17186061192223	27.574781807967845	20.72462341242493
21	26.003567519357844	24.610671284364884	28.81130949967858	20.574451696598693
22	23.898846926778983	20.976029281457585	29.84536888497646	25.279754906786973
23	23.244821015932217	23.571862928493246	28.64660130075462	24.536714754819915
24	22.8788607103765	23.744910783058916	28.306412847702827	25.069815658861756
25	24.34021161876191	24.363377328877796	25.569731683162583	25.726679369197715
26	22.34865552009915	22.412477051468414	26.779271322588304	28.45959610584413
27	22.334640265479038	21.70181097938831	29.429196902744557	26.534351852388095
28	20.09804886806549	23.006967087317353	29.238079794288495	27.65690425032866
29	22.73569662186032	22.547764798545195	28.20720569413155	26.50933288546294
30	24.20156484371833	21.957965818994722	29.367170713909275	24.473298623377676
31	25.16282598497704	23.923402579501822	27.144652485391124	23.769118950130018
32	25.85160046099763	24.12170105809381	25.448690847807075	24.578007633101485
33	24.873660008455484	22.31772929709444	26.177542002328153	26.63106869212192
34	21.673606727322216	23.450069207558972	29.251979220358027	25.62434484476078
35	21.316275648784668	25.59839924943099	26.704330250363412	26.380994851420926
36	23.45823512037482	25.00443044205966	25.628340929755776	25.90899350780974
37	22.353462404948196	26.350416114067958	26.65956251556446	24.636558965419386
38	23.24881710092721	25.239446571185333	28.148770190364225	23.362966137523237
39	22.900346906508986	23.51111085371433	27.46607671324905	26.122465526527634
40	23.882167615495543	23.6520741997695	28.749457053669158	23.716301131065794
41	20.282332092037368	24.632678708974975	26.022621315928163	29.062367883059498
42	20.819429081074194	23.442134951844277	27.81524187897075	27.92319408811078
43	21.39133254956014	21.707197006990253	28.38280177680997	28.51866866663964
44	23.0235884843255	21.47525033445494	28.520058609246597	26.981102571972965
45	22.562185453092333	21.563511689996467	26.487093803751687	29.387209053159513
46	22.40489028140546	22.62797606982145	28.292745078734455	26.674388570038627
47	19.433945873318315	24.089326978206856	30.790240286328174	25.68648686214665
48	20.4257278376547	24.918022343327408	28.999878380021894	25.656371438996
49	22.878281567623603	22.023524778622683	29.965656834753197	25.132536819000517
50	21.415424888080665	23.155285546334316	28.33096850042567	27.098321065159354
51	20.603177177142392	23.189976197232856	26.535741794995047	29.6711048306297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	1088.5
2	1903.0
3	5203.5
4	8504.0
5	5698.0
6	2892.0
7	2876.0
8	2860.0
9	2946.0
10	3032.0
11	3030.5
12	3029.0
13	2986.5
14	2944.0
15	3006.5
16	3069.0
17	3055.0
18	3041.0
19	2940.5
20	2840.0
21	3288.5
22	3737.0
23	4340.0
24	4943.0
25	5650.5
26	8305.0
27	10252.0
28	11371.0
29	12490.0
30	14998.5
31	17507.0
32	21721.5
33	25936.0
34	29858.0
35	33780.0
36	36765.5
37	39751.0
38	44426.5
39	49102.0
40	55212.5
41	61323.0
42	68072.0
43	74821.0
44	84973.5
45	95126.0
46	120801.5
47	146477.0
48	161141.5
49	175806.0
50	174750.0
51	173694.0
52	156520.0
53	139346.0
54	132072.0
55	124798.0
56	115610.0
57	106422.0
58	99772.0
59	93122.0
60	83955.5
61	74789.0
62	69296.5
63	63804.0
64	57373.5
65	50943.0
66	44158.5
67	37374.0
68	31788.5
69	26203.0
70	22034.0
71	17865.0
72	14482.5
73	11100.0
74	9333.0
75	5834.5
76	4103.0
77	3141.0
78	2179.0
79	1527.5
80	876.0
81	611.0
82	346.0
83	271.5
84	197.0
85	147.0
86	97.0
87	72.5
88	48.0
89	31.5
90	15.0
91	10.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1726690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.257325077967092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2535452773769	23.834818530824524
2	10.11753783870545	6.324943384260978
3	3.4957050171584805	3.277991642940095
4	1.6760724329672239	2.095581635659029
5	0.9825197355182551	1.5355469384306175
6	0.679772801771825	1.2748727666485449
7	0.49511315568177205	1.0833139000266294
8	0.3881714737048591	0.9706561551669068
9	0.32332566501259785	0.9095665875613796
>10	5.004725570715192	41.157981014156334
>50	0.49937232998079634	9.455157881471653
>100	0.07568810017088404	4.273323618336431
>500	0.00569497039809002	1.190711562276538
>1k	0.002571922115266461	2.319579164648956
>5k	1.837087225190329E-4	0.2959552175913039
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	5154	0.29849017484319706	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	4913	0.2845328344983755	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4741	0.27457157914854435	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	4263	0.24688855556005998	Illumina PCR Primer Index 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	4155	0.24063381382877064	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	4149	0.24028632817703235	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	3812	0.220769217404398	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	3632	0.2103446478522491	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2323	0.13453486149801064	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2171	0.12573189165397378	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.791427528971616E-5	0.0	0.0	0.30775645888955167	0.0
2	5.791427528971616E-5	0.0	0.0	1.1569534774626598	0.0
3	5.791427528971616E-5	0.0	0.0	1.6501514458298827	0.0
4	5.791427528971616E-5	0.0	0.0	2.305046070805993	0.0
5	5.791427528971616E-5	0.0	0.0	3.5215933375417707	0.0
6	5.791427528971616E-5	0.0	0.0	4.267471288998025	0.0
7	5.791427528971616E-5	0.0	0.0	4.969334391234096	0.0
8	1.1582855057943232E-4	0.0	0.0	6.036752399098854	0.0
9	1.1582855057943232E-4	0.0	0.0	6.618327551558184	0.0
10	1.1582855057943232E-4	0.0	0.0	7.7901649977703	0.0
11	1.1582855057943232E-4	0.0	0.0	9.388598995766467	0.0
12	1.1582855057943232E-4	0.0	0.0	11.077784663141617	0.0
13	1.1582855057943232E-4	0.0	0.0	11.580017258454037	0.0
14	1.1582855057943232E-4	0.0	0.0	11.77935819400124	0.0
15	1.1582855057943232E-4	0.0	0.0	12.086825081514343	0.0
16	1.1582855057943232E-4	0.0	0.0	12.685484945184138	0.0
17	1.1582855057943232E-4	0.0	0.0	13.458177206099531	0.0
18	1.1582855057943232E-4	0.0	0.0	14.304478510908154	0.0
19	1.737428258691485E-4	0.0	0.0	14.943330881629013	0.0
20	1.737428258691485E-4	0.0	0.0	15.59677764972288	0.0
21	1.737428258691485E-4	0.0	0.0	16.459179123062043	0.0
22	2.3165710115886465E-4	0.0	0.0	17.39049858399597	0.0
23	2.3165710115886465E-4	0.0	0.0	18.22712820483121	0.0
24	2.3165710115886465E-4	0.0	0.0	18.885266029223544	0.0
25	2.3165710115886465E-4	0.0	0.0	19.48323092158986	0.0
26	2.3165710115886465E-4	0.0	0.0	20.022065338885383	0.0
27	2.3165710115886465E-4	0.0	0.0	20.600571034754356	0.0
28	2.3165710115886465E-4	0.0	0.0	21.154289420799333	0.0
29	2.3165710115886465E-4	0.0	0.0	21.811153131135292	0.0
30	2.3165710115886465E-4	0.0	0.0	22.635504925609112	0.0
31	2.3165710115886465E-4	0.0	0.0	23.22796796182291	0.0
32	2.3165710115886465E-4	0.0	0.0	23.81759319854751	0.0
33	2.3165710115886465E-4	0.0	0.0	24.412141148671736	0.0
34	2.3165710115886465E-4	0.0	0.0	25.079603171385713	0.0
35	2.3165710115886465E-4	0.0	0.0	25.88710191175023	0.0
36	2.3165710115886465E-4	0.0	0.0	26.488773317735088	0.0
37	2.8957137644858083E-4	0.0	0.0	27.08442163908982	0.0
38	2.8957137644858083E-4	0.0	0.0	27.804180252390413	0.0
39	2.8957137644858083E-4	0.0	0.0	29.036190630628543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCGG	20	7.034271E-4	45.000004	13
TATACCG	20	7.034271E-4	45.000004	39
TACGGGA	260	0.0	42.403843	4
TATGGGA	840	0.0	39.642857	4
CGTTTTT	2675	0.0	39.364483	1
TGGGCGA	2620	0.0	38.387405	6
CGTTGAT	200	0.0	38.250004	25
ACATACG	265	0.0	38.207546	17
TACGGGT	125	0.0	37.8	4
TTACGGG	520	0.0	37.211536	3
TTTGGGC	4210	0.0	37.143707	4
TTTGGGA	7280	0.0	37.057003	4
TTGGGAC	3105	0.0	36.884056	5
TCGTTGA	215	0.0	36.627907	24
TTAATCG	215	0.0	36.627907	20
ATACGAA	265	0.0	36.509434	19
TTGGGAT	3340	0.0	36.377247	5
TTTACGG	310	0.0	36.29032	2
GTCGAAC	25	0.002107401	36.000004	21
TAAACCG	25	0.002107401	36.000004	11
>>END_MODULE