##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933145.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969919
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.170168849151324	33.0	31.0	34.0	30.0	34.0
2	32.356624625355316	34.0	31.0	34.0	30.0	34.0
3	32.422784789245284	34.0	31.0	34.0	30.0	34.0
4	35.91809316035669	37.0	35.0	37.0	35.0	37.0
5	35.12891179572727	37.0	35.0	37.0	35.0	37.0
6	34.82271921675934	37.0	35.0	37.0	32.0	37.0
7	35.61723504746273	37.0	35.0	37.0	33.0	37.0
8	35.90034013149552	37.0	35.0	37.0	35.0	37.0
9	37.762265714972074	39.0	38.0	39.0	35.0	39.0
10	37.23590939037177	39.0	37.0	39.0	34.0	39.0
11	37.167522236392934	39.0	37.0	39.0	34.0	39.0
12	36.96334023768995	39.0	37.0	39.0	33.0	39.0
13	36.8683302420099	39.0	37.0	39.0	33.0	39.0
14	37.99040229132536	40.0	37.0	41.0	33.0	41.0
15	38.10710482009323	40.0	37.0	41.0	33.0	41.0
16	38.1087554733952	40.0	37.0	41.0	33.0	41.0
17	38.06699734720116	40.0	37.0	41.0	33.0	41.0
18	38.01933769727163	40.0	37.0	41.0	33.0	41.0
19	38.011698915064045	40.0	37.0	41.0	34.0	41.0
20	37.920057241893396	40.0	36.0	41.0	33.0	41.0
21	37.80811284241261	40.0	36.0	41.0	33.0	41.0
22	37.84117539712079	40.0	36.0	41.0	33.0	41.0
23	37.75942836463663	40.0	36.0	41.0	33.0	41.0
24	37.69677880317841	39.0	36.0	41.0	33.0	41.0
25	37.55983953299193	39.0	35.0	41.0	33.0	41.0
26	37.504897831674604	39.0	35.0	41.0	33.0	41.0
27	37.468156619264086	39.0	35.0	41.0	33.0	41.0
28	37.38753854703331	39.0	35.0	41.0	33.0	41.0
29	37.32205988335108	39.0	35.0	41.0	32.0	41.0
30	37.23279572830309	39.0	35.0	41.0	32.0	41.0
31	37.14190257124564	39.0	35.0	41.0	32.0	41.0
32	37.093731538406814	39.0	35.0	41.0	32.0	41.0
33	36.985967900412305	39.0	35.0	41.0	31.0	41.0
34	36.85568794919988	39.0	35.0	41.0	31.0	41.0
35	36.70460213687947	39.0	35.0	41.0	31.0	41.0
36	36.55426380965833	39.0	35.0	40.0	31.0	41.0
37	36.49055745892183	39.0	35.0	40.0	31.0	41.0
38	36.3864807267411	39.0	35.0	40.0	30.0	41.0
39	36.33185142264457	38.0	35.0	40.0	30.0	41.0
40	36.215499438612916	38.0	35.0	40.0	30.0	41.0
41	36.07694250757022	38.0	35.0	40.0	30.0	41.0
42	36.010077130152105	38.0	35.0	40.0	30.0	41.0
43	35.841049613421326	38.0	35.0	40.0	29.0	41.0
44	35.7452952256838	38.0	35.0	40.0	29.0	41.0
45	35.72396354747149	38.0	34.0	40.0	29.0	41.0
46	35.49445056752162	38.0	34.0	40.0	28.0	41.0
47	35.36879368277145	37.0	34.0	40.0	28.0	41.0
48	35.276460199253755	37.0	34.0	40.0	28.0	41.0
49	35.280880155971786	37.0	34.0	40.0	28.0	41.0
50	35.045668762030644	36.0	34.0	40.0	28.0	41.0
51	33.93848455386481	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	31.0
11	11.0
12	9.0
13	15.0
14	24.0
15	36.0
16	46.0
17	89.0
18	200.0
19	360.0
20	683.0
21	1071.0
22	1687.0
23	2531.0
24	3672.0
25	5381.0
26	7582.0
27	9435.0
28	11602.0
29	13735.0
30	17360.0
31	22109.0
32	29204.0
33	41314.0
34	69312.0
35	90153.0
36	90285.0
37	132748.0
38	198126.0
39	221013.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.91424541636982	4.639975090703451	54.01152054965414	16.434258943272585
2	21.368588510999373	4.261696079775734	53.123198947540985	21.246516461683914
3	22.06070816222798	4.578320457687704	51.56461518951583	21.796356190568492
4	18.793631220751422	4.98835469766032	50.75506305165689	25.462951029931364
5	17.233397840438222	7.9332397860027495	50.22398777629885	24.609374597260185
6	22.046377068600574	7.773432626848222	53.02143787264709	17.158752431904105
7	85.07081519178405	2.201524044791369	8.44441649251123	4.283244270913345
8	87.17666114386871	2.573307667959902	6.449816943476723	3.80021424469466
9	81.20379124442351	5.23404531718628	8.577623492271004	4.984539946119212
10	40.83774005870593	26.096612191327317	15.752655634130273	17.312992115836476
11	31.247867089932253	23.884984210021663	24.023140076645575	20.844008623400512
12	26.603458639329673	20.999485524048918	31.882146859686223	20.514908976935185
13	23.692081503713197	23.68445200063098	32.59550539787343	20.02796109778239
14	21.089802344319473	25.20447583767304	32.0790705203218	21.626651297685683
15	19.141392219350276	21.930594204258295	36.50593503168821	22.42207854470322
16	21.83027654886645	20.901023693731126	33.45320588626473	23.815493871137694
17	22.37238367327581	19.725152306532813	29.990339399475623	27.91212462071575
18	23.49629195840065	20.82524416987398	31.158993689163733	24.519470182561637
19	24.473280758496326	23.37421990908519	28.82570606411463	23.326793268303845
20	28.03007261431109	22.1073099918653	27.663134756613694	22.19948263720991
21	26.65439072747312	23.235960941068274	27.081952204256233	23.027696127202375
22	25.03559575593426	22.457648525289226	26.86760440820316	25.639151310573354
23	24.480188551827524	23.96571260074295	25.83741528931797	25.71668355811155
24	24.097166876821674	21.741300046704932	29.457614501829532	24.70391857464386
25	23.05924515346127	23.496188857007645	28.197921682119848	25.246644307411238
26	22.692719701335882	24.71154807772608	27.747884101662095	24.847848119275938
27	23.181214101383723	23.620426035576166	28.742193935782268	24.456165927257842
28	21.456224695051855	23.38886030689161	30.36748429508031	24.78743070297623
29	23.008209963924823	23.28874885428577	28.58599532538284	25.117045856406566
30	24.40461523075638	23.683833392272962	28.109666889709345	23.801884487261308
31	26.02155437722119	25.72854021830689	24.727528793641532	23.52237661083039
32	25.54316391368764	25.30108184291678	24.73845754129984	24.417296702095744
33	25.636573775748282	24.02540830729164	25.24148923776109	25.096528679198986
34	23.781676614232737	23.817452797604748	27.114326041659147	25.28654454650337
35	24.559370421653767	24.61452966691033	25.85494252612847	24.971157385307432
36	26.97276782906614	24.229652166830427	25.692454730755866	23.105125273347568
37	23.94602023467939	26.73233538058333	25.51264590135877	23.80899848337851
38	22.586731469328882	27.222891808491223	25.396244428658477	24.794132293521418
39	23.955093157263647	25.69162991961184	26.41406138038331	23.9392155427412
40	24.962909273867197	24.67175094002695	26.46798340892384	23.89735637718201
41	21.775735911967907	23.92725578115286	27.21938636112913	27.077621945750106
42	23.603311204337682	23.627540031693368	26.65748376926321	26.11166499470574
43	24.462352010838018	22.869950995907907	27.649525372737312	25.018171620516767
44	22.78262411603443	24.50895384047534	26.855129139649808	25.853292903840426
45	23.38019978987936	24.326670577646176	26.05258789651507	26.240541735959393
46	24.72959082150159	23.722496414649058	27.199590893672564	24.348321870176786
47	22.36320764929855	24.986828797043877	28.284733055028305	24.365230498629266
48	23.293903923935915	23.17853346516565	28.232048243203817	25.295514367694622
49	23.054090083811122	22.633436400359205	29.474626231675018	24.837847284154655
50	21.87306362696266	24.432761911046182	27.60776930857113	26.086405153420028
51	21.92946008893526	23.81683418924673	26.62974949454542	27.62395622727259
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	603.5
2	1070.0
3	2704.5
4	4339.0
5	3044.0
6	1749.0
7	1716.5
8	1684.0
9	1787.5
10	1891.0
11	1910.0
12	1929.0
13	1932.0
14	1935.0
15	1906.5
16	1878.0
17	1815.5
18	1753.0
19	1985.5
20	2218.0
21	2313.5
22	2409.0
23	2883.0
24	3357.0
25	3782.5
26	5240.0
27	6272.0
28	7762.0
29	9252.0
30	9800.5
31	10349.0
32	13495.5
33	16642.0
34	18624.5
35	20607.0
36	22670.5
37	24734.0
38	28051.5
39	31369.0
40	34348.5
41	37328.0
42	42814.0
43	48300.0
44	49972.5
45	51645.0
46	56388.0
47	61131.0
48	70103.0
49	79075.0
50	85213.0
51	91351.0
52	90857.0
53	90363.0
54	81856.0
55	73349.0
56	67731.5
57	62114.0
58	57980.5
59	53847.0
60	50503.5
61	47160.0
62	42027.0
63	36894.0
64	32958.0
65	29022.0
66	24858.5
67	20695.0
68	17240.0
69	13785.0
70	11649.0
71	9513.0
72	8003.0
73	6493.0
74	4929.0
75	2749.0
76	2133.0
77	1751.5
78	1370.0
79	973.0
80	576.0
81	478.0
82	380.0
83	274.5
84	169.0
85	110.5
86	52.0
87	34.5
88	17.0
89	12.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	969919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.16037820016855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66021406911402	22.06280456961078
2	10.615287568453308	6.190916003992921
3	3.820591639481864	3.3422969146707957
4	1.759343749921816	2.0521251652729164
5	0.9551660054219557	1.3926500981024237
6	0.5860198777528972	1.0253136760854613
7	0.4247149583308273	0.8669394168537025
8	0.32223831001118447	0.7517272792407439
9	0.24896708018401992	0.6533976795801939
>10	4.315814054473333	34.58261464285701
>50	1.215315183572383	22.182995583242082
>100	0.07353928398442097	3.6592569751023056
>500	0.0010455822367453074	0.18598639812351514
>1k	0.0017426370612421789	1.0509755972651749
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2290	0.23610218997668878	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	2243	0.2312564245055515	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	2157	0.2223897047073003	No Hit
TCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	2055	0.21187336262100237	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1596	0.1645498232326617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24744334320701006	0.0
2	0.0	0.0	0.0	0.953584783884015	0.0
3	0.0	0.0	0.0	1.3539274929143568	0.0
4	0.0	0.0	0.0	1.7946859480018436	0.0
5	0.0	0.0	0.0	2.5540277074683555	0.0
6	0.0	0.0	0.0	3.0568532011436007	0.0
7	0.0	0.0	0.0	3.52029396269173	0.0
8	0.0	0.0	0.0	4.3126281679191765	0.0
9	0.0	0.0	0.0	4.769573541708122	0.0
10	0.0	0.0	0.0	5.556134068927405	0.0
11	1.0310139300292086E-4	0.0	0.0	6.812733846847005	0.0
12	1.0310139300292086E-4	0.0	0.0	7.956953106393421	0.0
13	1.0310139300292086E-4	0.0	0.0	8.310487783000436	0.0
14	1.0310139300292086E-4	0.0	0.0	8.430910210027848	0.0
15	1.0310139300292086E-4	0.0	0.0	8.622885003799286	0.0
16	1.0310139300292086E-4	0.0	0.0	9.133649304735757	0.0
17	1.0310139300292086E-4	0.0	0.0	9.910724503798772	0.0
18	1.0310139300292086E-4	0.0	0.0	10.7624451113959	0.0
19	1.0310139300292086E-4	0.0	0.0	11.26124965074403	0.0
20	1.0310139300292086E-4	0.0	0.0	11.820265403605868	0.0
21	1.0310139300292086E-4	0.0	0.0	12.555378335716695	0.0
22	1.0310139300292086E-4	0.0	0.0	13.324927133090496	0.0
23	1.0310139300292086E-4	0.0	0.0	14.086434021810069	0.0
24	1.0310139300292086E-4	0.0	0.0	14.66359561984042	0.0
25	1.0310139300292086E-4	0.0	0.0	15.165080795406627	0.0
26	1.0310139300292086E-4	0.0	0.0	15.622644777553589	0.0
27	1.0310139300292086E-4	0.0	0.0	16.071857546867317	0.0
28	1.0310139300292086E-4	0.0	0.0	16.51148188663177	0.0
29	1.0310139300292086E-4	0.0	0.0	17.01935934856416	0.0
30	1.0310139300292086E-4	0.0	0.0	17.614666791763025	0.0
31	1.0310139300292086E-4	0.0	0.0	18.156877017565385	0.0
32	1.0310139300292086E-4	0.0	0.0	18.639700840998064	0.0
33	1.0310139300292086E-4	0.0	0.0	19.14541317367739	0.0
34	1.0310139300292086E-4	0.0	0.0	19.646589045064587	0.0
35	2.0620278600584171E-4	0.0	0.0	20.201068336634297	0.0
36	2.0620278600584171E-4	0.0	0.0	20.698017050908376	0.0
37	2.0620278600584171E-4	0.0	0.0	21.17506719633289	0.0
38	2.0620278600584171E-4	0.0	0.0	21.670984896676938	0.0
39	3.093041790087626E-4	0.0	0.0	22.177006533535273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGCG	35	1.2116652E-7	45.000004	1
GGATACG	50	2.1827873E-11	45.000004	9
ACTAGCG	30	2.1651504E-6	45.000004	36
ACGTCCA	35	1.2116652E-7	45.000004	33
CGCGAAA	20	7.032742E-4	45.0	22
ACGGCAT	20	7.032742E-4	45.0	42
GTCACGA	20	7.032742E-4	45.0	38
TATTACG	20	7.032742E-4	45.0	1
ACTAACG	20	7.032742E-4	45.0	25
GTATCGC	20	7.032742E-4	45.0	32
CGTCCGA	20	7.032742E-4	45.0	35
TACCGGA	20	7.032742E-4	45.0	3
AAACACG	140	0.0	41.785717	40
TAAGCGA	60	3.6379788E-12	41.250004	18
CTAAGCG	55	6.002665E-11	40.909092	17
ATACTAT	660	0.0	40.56818	45
TTCTACG	90	0.0	40.0	1
TGCGTTG	180	0.0	40.0	1
CGATTAA	40	3.4574805E-7	39.375	24
TATCCGG	75	0.0	39.0	2
>>END_MODULE