##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933144.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1286278 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.280866189113084 33.0 31.0 34.0 30.0 34.0 2 32.46882477971325 34.0 31.0 34.0 31.0 34.0 3 32.54097014797734 34.0 31.0 34.0 31.0 34.0 4 35.99796467015684 37.0 35.0 37.0 35.0 37.0 5 35.20313415917865 37.0 35.0 37.0 35.0 37.0 6 34.888042864761736 37.0 35.0 37.0 32.0 37.0 7 35.67285687852859 37.0 35.0 37.0 35.0 37.0 8 35.955181539293996 37.0 35.0 37.0 35.0 37.0 9 37.832490332571965 39.0 38.0 39.0 35.0 39.0 10 37.34420241969465 39.0 37.0 39.0 34.0 39.0 11 37.25804919309822 39.0 37.0 39.0 34.0 39.0 12 37.084064253606144 39.0 37.0 39.0 34.0 39.0 13 37.029716748634435 39.0 37.0 39.0 33.0 39.0 14 38.212914315567865 40.0 37.0 41.0 33.0 41.0 15 38.30911358197839 40.0 37.0 41.0 34.0 41.0 16 38.31195200415462 40.0 37.0 41.0 34.0 41.0 17 38.274314728231374 40.0 37.0 41.0 34.0 41.0 18 38.22395780694376 40.0 37.0 41.0 34.0 41.0 19 38.22279320644526 40.0 37.0 41.0 34.0 41.0 20 38.13411408731238 40.0 37.0 41.0 34.0 41.0 21 38.01392545001936 40.0 37.0 41.0 33.0 41.0 22 38.04389564308804 40.0 37.0 41.0 34.0 41.0 23 37.968607874814 40.0 36.0 41.0 33.0 41.0 24 37.89240195354348 40.0 36.0 41.0 33.0 41.0 25 37.76728980826851 40.0 36.0 41.0 33.0 41.0 26 37.723350628713234 40.0 36.0 41.0 33.0 41.0 27 37.671140297820536 40.0 36.0 41.0 33.0 41.0 28 37.55716338147741 40.0 36.0 41.0 33.0 41.0 29 37.48774914909529 39.0 36.0 41.0 33.0 41.0 30 37.3735382242408 39.0 35.0 41.0 32.0 41.0 31 37.31138758495442 39.0 35.0 41.0 32.0 41.0 32 37.275103049263066 39.0 35.0 41.0 32.0 41.0 33 37.15576492795492 39.0 35.0 41.0 32.0 41.0 34 37.03746390749123 39.0 35.0 41.0 32.0 41.0 35 36.89669806993511 39.0 35.0 41.0 31.0 41.0 36 36.75290489303246 39.0 35.0 41.0 31.0 41.0 37 36.687831090946126 39.0 35.0 41.0 31.0 41.0 38 36.58224505122532 39.0 35.0 41.0 31.0 41.0 39 36.49941381256618 39.0 35.0 41.0 30.0 41.0 40 36.375924178132564 39.0 35.0 41.0 30.0 41.0 41 36.29273531849258 39.0 35.0 40.0 30.0 41.0 42 36.22357142079706 38.0 35.0 40.0 30.0 41.0 43 36.080691732269386 38.0 35.0 40.0 30.0 41.0 44 35.9839723605628 38.0 35.0 40.0 30.0 41.0 45 35.97223772776958 38.0 35.0 40.0 30.0 41.0 46 35.722401378240164 38.0 35.0 40.0 29.0 41.0 47 35.64481317413499 38.0 34.0 40.0 29.0 41.0 48 35.546996061504586 37.0 34.0 40.0 29.0 41.0 49 35.569652905514985 37.0 34.0 40.0 29.0 41.0 50 35.35301077994026 37.0 34.0 40.0 28.0 41.0 51 34.252850472448415 36.0 33.0 39.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 16.0 10 27.0 11 24.0 12 17.0 13 35.0 14 29.0 15 48.0 16 75.0 17 174.0 18 298.0 19 582.0 20 923.0 21 1562.0 22 2205.0 23 3257.0 24 4811.0 25 7188.0 26 9822.0 27 12254.0 28 14300.0 29 17287.0 30 21301.0 31 26598.0 32 35168.0 33 49406.0 34 82944.0 35 107735.0 36 115500.0 37 170241.0 38 265649.0 39 336682.0 40 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.095646508764045 4.394073442910475 53.429818437382906 18.08046161094258 2 20.365504191162408 4.3185843184754775 53.750122446314094 21.56578904404802 3 21.435568360805362 4.562466278673817 51.943592287203856 22.058373073316968 4 19.32296128830626 4.812489990499721 51.05575933040913 24.808789390784884 5 17.7187202144482 8.148316304873441 49.45905939462542 24.673904086052936 6 22.40479896258818 7.796137382432103 52.33689762244242 17.46216603253729 7 85.03612749343455 2.1894955833808867 8.639656435078576 4.134720488105993 8 87.16280617409299 2.6786588902243524 6.368685463018103 3.7898494726645406 9 81.56355002573316 5.187681045621553 8.365998641040273 4.8827702876050125 10 40.0984857083772 27.95686469021471 15.71495431003251 16.22969529137558 11 31.764206493464087 24.293115485143957 24.06097282236033 19.881705199031625 12 28.122381009392996 21.294308073371386 30.178934880329134 20.404376036906484 13 24.73742068199876 22.329232094461695 31.487516695457746 21.4458305280818 14 22.085039159497402 24.788576030997962 30.332556414709728 22.793828394794904 15 20.246789574259996 23.01151073096174 34.149149717246196 22.592549977532073 16 22.247212499941693 21.093029656108556 32.99527784817901 23.664479995770744 17 22.205464137612555 20.722425478784523 29.849534859493826 27.222575524109093 18 22.838531017400594 21.97860804585012 30.520618404419576 24.662242532329714 19 25.305260604628238 23.406370940030072 28.254078822773927 23.034289632567763 20 28.625693668087305 22.699292065945308 27.22692917083243 21.448085095134957 21 27.460937682211778 22.997983328642796 26.94044366769858 22.60063532144684 22 25.39404390030771 23.121595798108963 26.309631354963702 25.17472894661963 23 24.37218081938741 24.405532863035827 26.21198527845458 25.01030103912218 24 24.36090798412163 22.636941625371808 28.69029867571396 24.311851714792603 25 24.32289131898392 23.849354494129575 26.712265933180852 25.115488253705653 26 23.845000847406236 25.123029391780005 26.28327624354922 24.74869351726454 27 23.71757893705715 24.117570229763704 28.219249648987233 23.945601184191908 28 22.967741032653905 25.37009884332936 28.277246442837395 23.384913681179338 29 24.13583999726342 23.546542815783216 28.332055745336543 23.98556144161682 30 24.645449894968273 23.786071129258215 28.08918445312755 23.47929452264596 31 24.77100595672164 25.50358476161452 26.30387832179358 23.421530959870264 32 25.931641526948297 25.705407384717766 25.440534627817623 22.922416460516313 33 25.91065073024649 24.887466006570897 25.63738165466563 23.564501608516977 34 24.016814405595056 24.78328945997677 27.263390962140377 23.936505172287795 35 24.309830378813913 23.85417460300184 26.6863772839153 25.149617734268954 36 25.477929343423426 25.662492867016308 26.07593381835031 22.78364397120996 37 24.553168133171834 27.118554464897947 26.629313414362993 21.69896398756723 38 23.84119140652332 26.535865497194226 26.169692710285027 23.45325038599743 39 24.36261834533437 24.266760373729472 27.709639751282385 23.660981529653778 40 24.06859170412617 23.790735750747505 29.44246889086185 22.698203654264475 41 22.209662296952914 24.691629647712237 28.334854518230117 24.763853537104733 42 23.745411178609913 24.79464003893404 26.339484932495154 25.120463849960895 43 23.652585210973058 24.60478994431997 26.740253662116586 25.002371182590387 44 23.756761757567183 24.476435109673027 27.380472961521534 24.386330171238253 45 24.326856247249818 23.179514848267637 27.054104944654267 25.439523959828282 46 23.905018977235095 25.126761088971435 27.887983779556208 23.080236154237262 47 22.80206922609265 25.164389035651702 28.498038526663755 23.535503211591895 48 22.71942768204074 23.715713088461438 28.423249095452153 25.14161013404567 49 22.74803736050838 23.5519071304959 29.30735035505544 24.392705153940284 50 22.483864296831634 23.653129416813474 28.899895667966025 24.96311061838887 51 21.689090538748232 24.580533912575664 27.504707380519605 26.225668168156496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 240.0 1 1035.0 2 1830.0 3 4151.5 4 6473.0 5 4547.5 6 2622.0 7 2526.5 8 2431.0 9 2555.0 10 2679.0 11 2629.0 12 2579.0 13 2650.5 14 2722.0 15 2675.5 16 2629.0 17 2613.0 18 2597.0 19 2705.5 20 2814.0 21 3009.0 22 3204.0 23 4071.5 24 4939.0 25 5600.5 26 7980.5 27 9699.0 28 10524.0 29 11349.0 30 14539.0 31 17729.0 32 20447.5 33 23166.0 34 26207.5 35 29249.0 36 32342.5 37 35436.0 38 38029.0 39 40622.0 40 46482.0 41 52342.0 42 59548.5 43 66755.0 44 72535.5 45 78316.0 46 86672.0 47 95028.0 48 104721.0 49 114414.0 50 111315.0 51 108216.0 52 99351.0 53 90486.0 54 84501.5 55 78517.0 56 77032.0 57 75547.0 58 73574.5 59 71602.0 60 68145.5 61 64689.0 62 57068.5 63 49448.0 64 44129.0 65 38810.0 66 33571.0 67 28332.0 68 24639.0 69 20946.0 70 18577.5 71 16209.0 72 13535.5 73 10862.0 74 8664.0 75 4924.5 76 3383.0 77 2851.0 78 2319.0 79 1750.0 80 1181.0 81 934.0 82 687.0 83 476.0 84 265.0 85 189.5 86 114.0 87 79.5 88 45.0 89 32.5 90 20.0 91 12.0 92 4.0 93 3.5 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1286278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.30770606874177 #Duplication Level Percentage of deduplicated Percentage of total 1 76.87323936070705 23.298515430963395 2 10.297923657014568 6.242128866302797 3 3.341470473857329 3.038169148771416 4 1.421591917500627 1.7234075994123201 5 0.797632246992456 1.2087201846398699 6 0.5041914895531545 0.9168532480642857 7 0.38586627629014514 0.8186305178549135 8 0.29776113107107194 0.7219565471358513 9 0.23761021513863698 0.6481278503417075 >10 4.90351395420906 40.756905042236376 >50 0.8746350768650084 16.033992818376834 >100 0.06158431461443535 3.210946494156265 >500 0.0014899430932387486 0.30480811197096536 >1k 0.0014899430932387486 1.0768381397729807 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3316 0.2577980809747193 No Hit CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC 2569 0.1997235434330681 No Hit CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT 2444 0.19000558199704887 TruSeq Adapter, Index 27 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC 2420 0.18813973340133314 No Hit GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC 2368 0.18409706144394913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5548738297630838E-4 0.0 0.0 0.20508785814575076 0.0 2 1.5548738297630838E-4 0.0 0.0 0.8492720858165964 0.0 3 1.5548738297630838E-4 0.0 0.0 1.2323929974702204 0.0 4 1.5548738297630838E-4 0.0 0.0 1.6452897429638071 0.0 5 1.5548738297630838E-4 0.0 0.0 2.329822946517005 0.0 6 1.5548738297630838E-4 0.0 0.0 2.823106669009343 0.0 7 1.5548738297630838E-4 0.0 0.0 3.2562945179813383 0.0 8 1.5548738297630838E-4 0.0 0.0 3.9779114623743856 0.0 9 1.5548738297630838E-4 0.0 0.0 4.37743629293201 0.0 10 1.5548738297630838E-4 0.0 0.0 5.06904417241063 0.0 11 1.5548738297630838E-4 0.0 0.0 6.20542371089298 0.0 12 1.5548738297630838E-4 0.0 0.0 7.200776193015818 0.0 13 1.5548738297630838E-4 0.0 0.0 7.522324101010823 0.0 14 1.5548738297630838E-4 0.0 0.0 7.638239945019661 0.0 15 1.5548738297630838E-4 0.0 0.0 7.825835472580578 0.0 16 1.5548738297630838E-4 0.0 0.0 8.267264152850316 0.0 17 2.332310744644626E-4 0.0 0.0 8.904218217212764 0.0 18 2.332310744644626E-4 0.0 0.0 9.59053952567019 0.0 19 2.332310744644626E-4 0.0 0.0 10.018907265769919 0.0 20 2.332310744644626E-4 0.0 0.0 10.480627049518066 0.0 21 2.332310744644626E-4 0.0 0.0 11.125122251954865 0.0 22 2.332310744644626E-4 0.0 0.0 11.817041106199438 0.0 23 2.332310744644626E-4 0.0 0.0 12.484626185008217 0.0 24 3.1097476595261676E-4 0.0 0.0 13.016626265861657 0.0 25 3.8871845744077095E-4 0.0 0.0 13.483321645865047 0.0 26 3.8871845744077095E-4 0.0 0.0 13.901504962379828 0.0 27 4.664621489289252E-4 0.0 0.0 14.29527675976733 0.0 28 4.664621489289252E-4 0.0 0.0 14.72022377744158 0.0 29 4.664621489289252E-4 0.0 0.0 15.174868885264305 0.0 30 4.664621489289252E-4 0.0 0.0 15.692175408426483 0.0 31 4.664621489289252E-4 0.0 0.0 16.178539942376375 0.0 32 5.442058404170793E-4 0.0 0.0 16.650832868166912 0.0 33 5.442058404170793E-4 0.0 0.0 17.115351424808633 0.0 34 5.442058404170793E-4 0.0 0.0 17.57085171323773 0.0 35 5.442058404170793E-4 0.0 0.0 18.074708577772455 0.0 36 5.442058404170793E-4 0.0 0.0 18.546457297722576 0.0 37 5.442058404170793E-4 0.0 0.0 19.010198417449416 0.0 38 6.219495319052335E-4 0.0 0.0 19.456991412431837 0.0 39 6.219495319052335E-4 0.0 0.0 19.921509969073558 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGTA 20 7.0336007E-4 45.0 45 TACGATC 20 7.0336007E-4 45.0 12 ACTACGG 20 7.0336007E-4 45.0 32 GTATACG 20 7.0336007E-4 45.0 1 ATAACGC 20 7.0336007E-4 45.0 11 ATACGAT 20 7.0336007E-4 45.0 11 TATACGG 25 3.8910664E-5 45.0 2 TTCGAAT 25 3.8910664E-5 45.0 20 TCGAATC 25 3.8910664E-5 45.0 21 TACGGGT 100 0.0 42.75 4 GCGATCA 60 3.6379788E-12 41.249996 9 GCGATAT 50 1.0822987E-9 40.5 9 TTTGGGC 3640 0.0 39.00412 4 CCGTTTG 185 0.0 38.918922 1 TTTGGGA 6435 0.0 38.251747 4 ATAGCGC 30 1.1399976E-4 37.499996 36 GTTCACG 30 1.1399976E-4 37.499996 37 ACGTACC 30 1.1399976E-4 37.499996 11 TTGGGAC 2370 0.0 37.40506 5 TACGAAT 290 0.0 37.24138 12 >>END_MODULE