SRR2933143.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1081428
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2638	0.24393672070632533	TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2514	0.23247040024856025	TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	2463	0.227754413608673	TruSeq Adapter, Index 16 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2350	0.2173052667399032	No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2288	0.2115721065110206	TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	1187	0.10976227728521917	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAATCG	25	3.890651E-5	45.000004	34
TGTACGA	25	3.890651E-5	45.000004	21
ACGTTAT	20	7.0331024E-4	45.0	28
ATAGCGC	20	7.0331024E-4	45.0	36
CGATTAT	20	7.0331024E-4	45.0	10
TCGCTAT	20	7.0331024E-4	45.0	39
CGGTAAG	20	7.0331024E-4	45.0	20
AGCTACG	140	0.0	45.0	9
GCTACGA	150	0.0	42.0	10
TACGAAT	145	0.0	41.89655	12
TTACGCG	60	3.6379788E-12	41.250004	1
TTCCGAA	55	6.184564E-11	40.909092	44
TACGCGG	95	0.0	40.26316	2
CGTTTGG	740	0.0	39.83108	2
TCGTTCA	125	0.0	39.600002	16
TACGGGA	205	0.0	39.512196	4
TCCGATA	40	3.4579716E-7	39.375	14
TCGTACA	40	3.4579716E-7	39.375	34
ATACTAT	605	0.0	38.67769	45
TTTGGGC	2940	0.0	38.112244	4