##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1081428
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.186040124723974	33.0	31.0	34.0	30.0	34.0
2	32.365819083656056	34.0	31.0	34.0	30.0	34.0
3	32.45109521854437	34.0	31.0	34.0	30.0	34.0
4	35.94906457017943	37.0	35.0	37.0	35.0	37.0
5	35.149255428932854	37.0	35.0	37.0	35.0	37.0
6	34.82803478363793	37.0	35.0	37.0	32.0	37.0
7	35.611485924166935	37.0	35.0	37.0	33.0	37.0
8	35.88624115521329	37.0	35.0	37.0	35.0	37.0
9	37.76565615094116	39.0	38.0	39.0	35.0	39.0
10	37.24354187241314	39.0	37.0	39.0	34.0	39.0
11	37.16242505280056	39.0	37.0	39.0	34.0	39.0
12	36.962697470381755	39.0	37.0	39.0	33.0	39.0
13	36.87858276279142	39.0	37.0	39.0	33.0	39.0
14	37.996428795999364	40.0	37.0	41.0	33.0	41.0
15	38.08307256701325	40.0	37.0	41.0	33.0	41.0
16	38.10743479917294	40.0	37.0	41.0	33.0	41.0
17	38.06205498655481	40.0	37.0	41.0	33.0	41.0
18	38.041163165740116	40.0	37.0	41.0	33.0	41.0
19	38.02490503297492	40.0	37.0	41.0	34.0	41.0
20	37.937489134736666	40.0	36.0	41.0	33.0	41.0
21	37.83026516790762	40.0	36.0	41.0	33.0	41.0
22	37.859126081440465	40.0	36.0	41.0	33.0	41.0
23	37.770275043738465	40.0	36.0	41.0	33.0	41.0
24	37.69460565104658	40.0	36.0	41.0	33.0	41.0
25	37.571824476525485	39.0	35.0	41.0	33.0	41.0
26	37.52923449365099	39.0	35.0	41.0	33.0	41.0
27	37.467136046042825	39.0	35.0	41.0	33.0	41.0
28	37.3565554063701	39.0	35.0	41.0	32.0	41.0
29	37.30901086341393	39.0	35.0	41.0	32.0	41.0
30	37.23759048221426	39.0	35.0	41.0	32.0	41.0
31	37.137471010552716	39.0	35.0	41.0	32.0	41.0
32	37.11435250428138	39.0	35.0	41.0	32.0	41.0
33	37.01414888462293	39.0	35.0	41.0	31.0	41.0
34	36.888290297643486	39.0	35.0	41.0	31.0	41.0
35	36.77121361755013	39.0	35.0	41.0	31.0	41.0
36	36.617153430464164	39.0	35.0	41.0	31.0	41.0
37	36.5695617276416	39.0	35.0	41.0	31.0	41.0
38	36.44698306313504	39.0	35.0	41.0	30.0	41.0
39	36.40732716371316	39.0	35.0	40.0	30.0	41.0
40	36.29813357893452	39.0	35.0	40.0	30.0	41.0
41	36.189418990445965	38.0	35.0	40.0	30.0	41.0
42	36.08714588488554	38.0	35.0	40.0	30.0	41.0
43	35.90337035845197	38.0	35.0	40.0	29.0	41.0
44	35.81510188380549	38.0	35.0	40.0	29.0	41.0
45	35.805500689828634	38.0	35.0	40.0	29.0	41.0
46	35.5289413627167	38.0	34.0	40.0	28.0	41.0
47	35.45515651527425	37.0	34.0	40.0	28.0	41.0
48	35.37405541561713	37.0	34.0	40.0	28.0	41.0
49	35.41502254426555	37.0	34.0	40.0	28.0	41.0
50	35.204139341685256	37.0	34.0	40.0	27.0	41.0
51	34.10378869420803	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	26.0
11	11.0
12	18.0
13	17.0
14	14.0
15	27.0
16	42.0
17	114.0
18	237.0
19	394.0
20	735.0
21	1277.0
22	2002.0
23	2944.0
24	4369.0
25	6284.0
26	8716.0
27	10876.0
28	13072.0
29	15801.0
30	19516.0
31	24961.0
32	31897.0
33	44741.0
34	74637.0
35	97160.0
36	97392.0
37	140095.0
38	219050.0
39	264866.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.829088945357437	4.562670838927788	52.22344899521744	17.38479122049734
2	19.746945705123224	5.150134821735705	52.67877288178223	22.42414659135883
3	20.63956176462973	5.460372766379269	51.31132169686747	22.588743772123525
4	18.8660733770533	5.203582670321094	50.68261594854211	25.24772800408349
5	17.918529943741053	8.01301612312609	48.4694311595409	25.599022773591955
6	21.946352415509864	7.7295021027752195	52.21540407683174	18.108741404883173
7	84.12118051317333	2.3824054860795174	9.077442048846525	4.418971951900636
8	86.06296489456533	3.150371545770962	6.513609782620756	4.273053777042947
9	80.67388674974201	4.952340793839257	8.593359890811039	5.780412565607696
10	40.6657678550953	27.183039462636437	15.89259756544125	16.25859511682701
11	31.40995054686951	24.555125260303967	23.501333422104846	20.533590770721673
12	27.632167837340997	21.607633610374428	29.99552443620842	20.76467411607615
13	25.042813761064075	23.617846033207943	30.84689873019748	20.4924414755305
14	21.259667772611767	25.749841875742074	30.343027922339722	22.647462429306437
15	19.39148977093251	22.84072541121554	36.26334809159741	21.504436726254543
16	21.28611428592565	21.330037690904987	33.58383544720499	23.800012575964374
17	21.153419367724897	21.63371024238322	30.20866853826607	27.004201851625815
18	23.15891580391852	21.366193588477458	30.709765236335663	24.76512537126836
19	24.97540289321157	24.515547960659426	27.183131932962713	23.325917213166296
20	27.69060908354509	22.64320879429791	28.185232858775617	21.48094926338138
21	27.030740835265966	24.0165780800941	26.673805375854887	22.27887570878505
22	25.18040960655726	22.80577162788461	25.95031754310042	26.063501222457713
23	24.260422330474153	25.231730637638382	25.570726853752628	24.937120178134837
24	24.212430231138825	22.40112148011703	28.815788013626424	24.570660275117714
25	23.67416046190777	23.999840951038813	26.456962460746347	25.869036126307066
26	22.18908702197465	25.315693693893632	26.33887785409662	26.156341430035102
27	22.12167615412214	25.15803178759936	28.419737606202172	24.300554452076327
28	20.285215474354278	25.26936606043121	30.14708330096872	24.298335164245792
29	21.561675858217097	23.49911413427431	28.3468709890996	26.592339018408996
30	23.59213928250424	23.634768102915775	27.899961902225577	24.873130712354406
31	23.236036056029622	25.96400315138872	25.353144176033908	25.44681661654775
32	23.369470736840547	25.480290874658323	25.98138757272791	25.16885081577322
33	23.188783719304475	24.417899296115877	25.597543248371597	26.79577373620805
34	20.567804791442427	25.073328968733932	27.291599625680117	27.06726661414352
35	21.435176451876593	23.379272591425412	27.503264202517414	27.682286754180584
36	22.35322185110798	25.70083260281776	25.562034643083038	26.383910902991232
37	21.625110502039895	26.294307156833373	27.010674774464878	25.069907566661858
38	21.002045443617142	26.10206134851326	26.643197697858756	26.252695510010838
39	21.616510761696574	24.259405156885155	26.22550923408678	27.898574847331492
40	22.723473037502266	22.576630159381857	27.880635604034666	26.81926119908121
41	19.972665771553906	23.19664369703762	27.93676509208195	28.893925439326516
42	21.492692994817965	23.882958458630625	27.585285381920944	27.039063164630473
43	22.104476673435496	23.405256753107928	27.21725348335719	27.273013090099386
44	21.611055012446506	23.86871802838469	27.325721176074598	27.194505783094204
45	21.800526710978446	23.170289654050016	25.770277817848253	29.25890581712329
46	21.44109455275802	24.28779354705075	27.43400392813946	26.837107972051772
47	20.126906275776104	24.406525445984386	29.152472471583867	26.314095806655647
48	20.452309353928324	22.69739640549348	29.274625772589573	27.575668467988624
49	20.400803382194653	22.60991947683988	30.011799213632344	26.977477927333123
50	20.532573597132682	23.309827376394914	27.96487607126873	28.192722955203674
51	19.225690475926275	23.220038689584513	27.368072585507306	30.1861982489819
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	743.0
2	1304.0
3	2986.5
4	4669.0
5	3273.0
6	1877.0
7	1991.5
8	2106.0
9	2135.0
10	2164.0
11	2120.5
12	2077.0
13	1937.0
14	1797.0
15	1763.5
16	1730.0
17	1847.0
18	1964.0
19	2184.0
20	2404.0
21	2384.0
22	2364.0
23	2583.5
24	2803.0
25	3829.0
26	6070.0
27	7285.0
28	8764.5
29	10244.0
30	12299.0
31	14354.0
32	16693.5
33	19033.0
34	21590.0
35	24147.0
36	25368.5
37	26590.0
38	30325.5
39	34061.0
40	38805.5
41	43550.0
42	47630.0
43	51710.0
44	56578.0
45	61446.0
46	66429.5
47	71413.0
48	82066.0
49	92719.0
50	99657.5
51	106596.0
52	103212.0
53	99828.0
54	88693.5
55	77559.0
56	72248.5
57	66938.0
58	60831.0
59	54724.0
60	52029.5
61	49335.0
62	44952.5
63	40570.0
64	35226.0
65	29882.0
66	25396.5
67	20911.0
68	18386.5
69	15862.0
70	13561.5
71	11261.0
72	9543.5
73	7826.0
74	6384.0
75	3976.5
76	3011.0
77	2400.0
78	1789.0
79	1312.5
80	836.0
81	609.5
82	383.0
83	303.0
84	223.0
85	150.5
86	78.0
87	47.0
88	16.0
89	10.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1081428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.71900478475586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73481842035417	22.50763431007122
2	10.73408242802288	6.380124940767515
3	3.763983393705633	3.355855214618379
4	1.729781055299515	2.0562948583610527
5	0.9466690484795206	1.4067030990671574
6	0.6333103471434021	1.1292811942194094
7	0.44871770933909083	0.9334810625597191
8	0.3267061021723118	0.7767504170934296
9	0.2563715218509047	0.6857195836105962
>10	4.2061284405546635	33.8174800169958
>50	1.1350703685580297	21.096934393658426
>100	0.08009742335281177	4.085173497129657
>500	0.0021318705835273997	0.4595197927303211
>1k	0.0021318705835273997	1.3090476191173201
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2638	0.24393672070632533	TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2514	0.23247040024856025	TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	2463	0.227754413608673	TruSeq Adapter, Index 16 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2350	0.2173052667399032	No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2288	0.2115721065110206	TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	1187	0.10976227728521917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26566724738031566	0.0
2	0.0	0.0	0.0	1.095218544369112	0.0
3	0.0	0.0	0.0	1.5416652796117727	0.0
4	0.0	0.0	0.0	2.059499106736648	0.0
5	0.0	0.0	0.0	2.971996286391697	0.0
6	0.0	0.0	0.0	3.535510454695088	0.0
7	0.0	0.0	0.0	4.095510750600132	0.0
8	0.0	0.0	0.0	4.997466313060139	0.0
9	0.0	0.0	0.0	5.493569613511024	0.0
10	0.0	0.0	0.0	6.431496132890955	0.0
11	0.0	0.0	0.0	7.7675074068731345	0.0
12	0.0	0.0	0.0	9.151510780190637	0.0
13	0.0	0.0	0.0	9.543584963585186	0.0
14	0.0	0.0	0.0	9.6757250598283	0.0
15	0.0	0.0	0.0	9.921696127712616	0.0
16	9.247032627229922E-5	0.0	0.0	10.443783589846019	0.0
17	9.247032627229922E-5	0.0	0.0	11.201023091689876	0.0
18	9.247032627229922E-5	0.0	0.0	11.998579655788458	0.0
19	9.247032627229922E-5	0.0	0.0	12.514194195082798	0.0
20	9.247032627229922E-5	0.0	0.0	13.066242042928424	0.0
21	9.247032627229922E-5	0.0	0.0	13.80609712343309	0.0
22	9.247032627229922E-5	0.0	0.0	14.586639147497568	0.0
23	9.247032627229922E-5	0.0	0.0	15.35774919828227	0.0
24	9.247032627229922E-5	0.0	0.0	15.96176536949293	0.0
25	9.247032627229922E-5	0.0	0.0	16.465913588329506	0.0
26	9.247032627229922E-5	0.0	0.0	16.890722267224447	0.0
27	9.247032627229922E-5	0.0	0.0	17.349097674556234	0.0
28	1.8494065254459844E-4	0.0	0.0	17.81144930591773	0.0
29	1.8494065254459844E-4	0.0	0.0	18.299322747330383	0.0
30	1.8494065254459844E-4	0.0	0.0	18.89806810994352	0.0
31	1.8494065254459844E-4	0.0	0.0	19.415347115110762	0.0
32	1.8494065254459844E-4	0.0	0.0	19.922731795366868	0.0
33	1.8494065254459844E-4	0.0	0.0	20.42484566702545	0.0
34	1.8494065254459844E-4	0.0	0.0	20.91503086659491	0.0
35	1.8494065254459844E-4	0.0	0.0	21.484463135779727	0.0
36	1.8494065254459844E-4	0.0	0.0	21.996656273001992	0.0
37	1.8494065254459844E-4	0.0	0.0	22.516709387957405	0.0
38	1.8494065254459844E-4	0.0	0.0	23.016234090480364	0.0
39	2.7741097881689767E-4	0.0	0.0	23.546089060020638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAATCG	25	3.890651E-5	45.000004	34
TGTACGA	25	3.890651E-5	45.000004	21
ACGTTAT	20	7.0331024E-4	45.0	28
ATAGCGC	20	7.0331024E-4	45.0	36
CGATTAT	20	7.0331024E-4	45.0	10
TCGCTAT	20	7.0331024E-4	45.0	39
CGGTAAG	20	7.0331024E-4	45.0	20
AGCTACG	140	0.0	45.0	9
GCTACGA	150	0.0	42.0	10
TACGAAT	145	0.0	41.89655	12
TTACGCG	60	3.6379788E-12	41.250004	1
TTCCGAA	55	6.184564E-11	40.909092	44
TACGCGG	95	0.0	40.26316	2
CGTTTGG	740	0.0	39.83108	2
TCGTTCA	125	0.0	39.600002	16
TACGGGA	205	0.0	39.512196	4
TCCGATA	40	3.4579716E-7	39.375	14
TCGTACA	40	3.4579716E-7	39.375	34
ATACTAT	605	0.0	38.67769	45
TTTGGGC	2940	0.0	38.112244	4
>>END_MODULE