SRR2933142.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618012
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2861	0.4629359947703281	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2589	0.418923904390206	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2504	0.4051701261464179	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2442	0.395137958486243	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1862	0.301288648116865	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	849	0.13737597328207218	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	664	0.10744127945735682	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.0307514E-4	45.000004	12
CTCCGTA	20	7.0307514E-4	45.000004	16
CTATACG	20	7.0307514E-4	45.000004	1
ATTCGAT	20	7.0307514E-4	45.000004	37
CGACATA	20	7.0307514E-4	45.000004	2
TGCGGTA	20	7.0307514E-4	45.000004	4
CGCGAGT	20	7.0307514E-4	45.000004	17
CGACAAG	20	7.0307514E-4	45.000004	22
ACGATAC	20	7.0307514E-4	45.000004	45
ACGGCTT	20	7.0307514E-4	45.000004	19
GTTCGAC	20	7.0307514E-4	45.000004	37
TAATTCG	20	7.0307514E-4	45.000004	24
CGTACTT	20	7.0307514E-4	45.000004	15
AACCTCG	20	7.0307514E-4	45.000004	38
TATACGG	20	7.0307514E-4	45.000004	2
AATACGG	20	7.0307514E-4	45.000004	27
TCGGTAC	20	7.0307514E-4	45.000004	36
TACGTAC	20	7.0307514E-4	45.000004	13
CGGGTCG	25	3.888705E-5	45.0	6
AACCGCG	25	3.888705E-5	45.0	26