##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618012
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12592959360013	33.0	31.0	34.0	30.0	34.0
2	32.30893413072885	34.0	31.0	34.0	30.0	34.0
3	32.39067849815214	34.0	31.0	34.0	30.0	34.0
4	35.88446664466062	37.0	35.0	37.0	35.0	37.0
5	35.14607159731526	37.0	35.0	37.0	35.0	37.0
6	34.82827841530585	37.0	35.0	37.0	32.0	37.0
7	35.634007106658125	37.0	35.0	37.0	33.0	37.0
8	35.89612823051979	37.0	35.0	37.0	35.0	37.0
9	37.77127790398892	39.0	38.0	39.0	35.0	39.0
10	37.26719060471318	39.0	37.0	39.0	34.0	39.0
11	37.15850339475609	39.0	37.0	39.0	34.0	39.0
12	36.88100069254319	39.0	35.0	39.0	33.0	39.0
13	36.73246150560183	39.0	35.0	39.0	33.0	39.0
14	37.82563283560837	40.0	36.0	41.0	33.0	41.0
15	37.94418231361203	40.0	36.0	41.0	33.0	41.0
16	37.98061040885938	40.0	36.0	41.0	33.0	41.0
17	37.93991055189867	40.0	36.0	41.0	33.0	41.0
18	37.91252273418639	40.0	36.0	41.0	33.0	41.0
19	37.924376872940975	40.0	36.0	41.0	34.0	41.0
20	37.83755007993372	40.0	36.0	41.0	33.0	41.0
21	37.69016459227329	39.0	36.0	41.0	33.0	41.0
22	37.74861329553472	39.0	36.0	41.0	33.0	41.0
23	37.68421486961418	39.0	35.0	41.0	33.0	41.0
24	37.65196468676984	39.0	35.0	41.0	33.0	41.0
25	37.53717565354718	39.0	35.0	41.0	33.0	41.0
26	37.48052950428147	39.0	35.0	41.0	33.0	41.0
27	37.38913322071416	39.0	35.0	41.0	33.0	41.0
28	37.308736723558766	39.0	35.0	41.0	33.0	41.0
29	37.2393934098367	39.0	35.0	41.0	32.0	41.0
30	37.13130974803078	39.0	35.0	41.0	32.0	41.0
31	37.07951787343935	39.0	35.0	41.0	32.0	41.0
32	37.04322731597445	39.0	35.0	41.0	32.0	41.0
33	36.91931062827259	39.0	35.0	41.0	31.0	41.0
34	36.83650155660408	39.0	35.0	41.0	31.0	41.0
35	36.71288259774891	39.0	35.0	41.0	31.0	41.0
36	36.54657838359126	39.0	35.0	40.0	31.0	41.0
37	36.483019747189374	39.0	35.0	40.0	31.0	41.0
38	36.404628065474455	38.0	35.0	40.0	30.0	41.0
39	36.36874526708219	38.0	35.0	40.0	30.0	41.0
40	36.33014569296389	38.0	35.0	40.0	30.0	41.0
41	36.27728425985256	38.0	35.0	40.0	30.0	41.0
42	36.198331100366985	38.0	35.0	40.0	30.0	41.0
43	35.99600331385151	38.0	35.0	40.0	30.0	41.0
44	35.898877368076995	38.0	35.0	40.0	30.0	41.0
45	35.87119182151803	38.0	35.0	40.0	30.0	41.0
46	35.643794942493024	38.0	34.0	40.0	29.0	41.0
47	35.58956460392355	38.0	34.0	40.0	29.0	41.0
48	35.50779272894378	38.0	34.0	40.0	28.0	41.0
49	35.58983644330531	37.0	34.0	40.0	29.0	41.0
50	35.365476722134844	37.0	34.0	40.0	28.0	41.0
51	34.32489984013255	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	16.0
11	16.0
12	9.0
13	10.0
14	16.0
15	16.0
16	36.0
17	65.0
18	101.0
19	233.0
20	399.0
21	692.0
22	1067.0
23	1586.0
24	2334.0
25	3392.0
26	4630.0
27	5858.0
28	7348.0
29	8698.0
30	11175.0
31	13959.0
32	18736.0
33	26094.0
34	45415.0
35	61546.0
36	54406.0
37	78822.0
38	125016.0
39	146245.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.113408801123605	4.794405286628738	51.6020724516676	17.490113460580055
2	20.610117602894444	4.761396218843647	52.60771635502223	22.02076982323968
3	21.441654854598294	4.694730846650227	51.78362232448561	22.079991974265873
4	19.374866507446455	4.426289457162643	51.04496352821628	25.15388050717462
5	17.342705319637805	7.939813466405184	50.52005462677101	24.197426587186012
6	21.442302091221528	7.856643560319217	53.21077907872339	17.490275269735864
7	82.8684556286933	2.0009320207374612	10.258538669152054	4.872073681417189
8	84.46146676763559	2.6324731558610512	8.001624563924326	4.9044355125790435
9	78.93341876856759	4.941489809259368	10.296078393299807	5.829013028873225
10	41.14758289483052	25.363261554791816	17.434289301825853	16.054866248551807
11	31.936758509543505	24.249690944512402	24.627515323327053	19.186035222617036
12	27.465486107065885	21.3039552630046	31.531588383397086	19.69897024653243
13	23.317832016206804	25.398535950758237	32.42655482417817	18.857077208856786
14	19.85867588331618	27.797356685630696	31.70682769913853	20.63713973191459
15	17.85709662595548	23.59954175647075	38.446664466062145	20.096697151511624
16	19.291696601360492	22.113486469518392	35.08637372737099	23.50844320175013
17	19.48700025242228	21.6092891400167	29.78259321825466	29.121117389306356
18	20.81108457440956	21.73113143434108	33.582195814967996	23.87558817628137
19	23.54824825407921	23.160068089292764	30.31607800495783	22.975605651670193
20	26.239134515187406	23.24760684258558	28.89183381552462	21.62142482670239
21	24.576545439247134	24.887542636712556	29.536966919736184	20.998945004304122
22	23.912966091273308	22.52043649637871	28.620479861232468	24.946117551115513
23	21.307029636964977	25.30080322064944	27.540403746205577	25.85176339618001
24	22.627877775836065	22.48726561943781	30.886778897497138	23.998077707228983
25	20.914157006660066	23.907626389131604	29.437454288913482	25.740762315294845
26	21.11124055843576	25.49643049002285	28.78989404736478	24.60243490417662
27	20.318699313281943	25.64691300492547	30.26980058639638	23.764587095396205
28	19.25059707578494	23.938531937891174	32.30778690381417	24.503084082509723
29	20.011909153867563	23.675915678012725	30.819304479524668	25.492870688595044
30	21.216416509711784	25.9982006821874	30.431124314738224	22.35425849336259
31	22.87382769266616	25.842054846831452	27.25788496016259	24.026232500339802
32	22.981592590435138	26.500132683507765	25.917457913438575	24.600816812618525
33	22.21413176443176	25.610344135712577	26.79009469071798	25.38542940913769
34	20.909626350297405	23.70439408943516	28.920797654414475	26.46518190585296
35	20.655747784832656	24.356484987346523	28.197672537102843	26.79009469071798
36	23.153919341372013	24.305029675799176	28.702355294071957	23.838695688756854
37	19.824534151440425	26.148197769622595	30.082263774813434	23.945004304123543
38	18.828275179122738	28.8025151615179	27.016303890539344	25.352905768820023
39	19.94168398024634	27.21727086205446	28.5617431376737	24.2793020200255
40	21.63841478806237	23.627211122114133	29.755894707546133	24.978479382277367
41	19.2342543510482	22.88563976104024	28.952026821485667	28.928079066425894
42	21.34845278085215	22.072225134787026	27.24316032698394	29.33616175737688
43	21.34456936111273	22.870753318705788	28.176151919380203	27.60852540080128
44	20.62888746496832	24.144676802392187	28.469997346329844	26.75643838630965
45	20.94635702866611	24.885600926842844	27.631178682614575	26.536863361876467
46	22.22028051235251	24.34677643799797	29.459783952415165	23.97315909723436
47	19.397196170948135	24.037882759558066	30.55248118159518	26.01243988789862
48	19.523892739946795	23.200196759933466	30.71283405500217	26.563076445117574
49	21.099266680905874	21.764949547905218	31.471233568280226	25.664550202908682
50	20.238118353688925	23.286926467447234	29.80039222539368	26.67456295347016
51	18.95885516786082	23.051170527433122	27.499627838941638	30.490346465764418
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	377.5
2	661.0
3	1907.5
4	3154.0
5	2067.5
6	981.0
7	1029.5
8	1078.0
9	1160.0
10	1242.0
11	1285.0
12	1328.0
13	1356.5
14	1385.0
15	1421.0
16	1457.0
17	1516.5
18	1576.0
19	1574.0
20	1572.0
21	1764.5
22	1957.0
23	1856.0
24	1755.0
25	2551.0
26	4057.5
27	4768.0
28	5246.0
29	5724.0
30	7127.5
31	8531.0
32	9389.0
33	10247.0
34	11759.5
35	13272.0
36	14648.0
37	16024.0
38	18332.0
39	20640.0
40	23956.0
41	27272.0
42	32385.0
43	37498.0
44	42005.0
45	46512.0
46	52588.0
47	58664.0
48	64345.5
49	70027.0
50	68144.5
51	66262.0
52	56572.0
53	46882.0
54	41317.5
55	35753.0
56	33182.0
57	30611.0
58	27649.0
59	24687.0
60	22150.5
61	19614.0
62	18197.0
63	16780.0
64	14686.0
65	12592.0
66	10586.0
67	8580.0
68	6937.5
69	5295.0
70	4834.5
71	4374.0
72	3499.5
73	2625.0
74	2086.0
75	1209.5
76	872.0
77	656.5
78	441.0
79	313.5
80	186.0
81	153.5
82	121.0
83	68.0
84	15.0
85	9.5
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	618012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.967713870811398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2422516483104	22.681450922988404
2	11.569781397494731	7.165793597309064
3	4.600800804471216	4.274288486683905
4	2.146133763025695	2.6584342520746986
5	1.1920182762313216	1.8457040453554694
6	0.7917478818763731	1.47111731182611
7	0.5374979168690308	1.1651557187030122
8	0.39025924229507186	0.9668349240666754
9	0.2993303925308113	0.8342620153858641
>10	4.419381997901645	36.59260324771884
>50	0.7248188820567604	13.789030936074756
>100	0.08233025403662772	4.333156014211056
>500	0.0010421551143876926	0.24414646592564432
>1k	0.0026053877859692313	1.9780220616765025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2861	0.4629359947703281	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2589	0.418923904390206	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2504	0.4051701261464179	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2442	0.395137958486243	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1862	0.301288648116865	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	849	0.13737597328207218	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	664	0.10744127945735682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.618091558092723E-4	0.0	0.0	0.506300848527213	0.0
2	1.618091558092723E-4	0.0	0.0	1.8625851925205337	0.0
3	1.618091558092723E-4	0.0	0.0	2.560953508993353	0.0
4	1.618091558092723E-4	0.0	0.0	3.3047901982485777	0.0
5	1.618091558092723E-4	0.0	0.0	4.545542804994078	0.0
6	1.618091558092723E-4	0.0	0.0	5.303133272493091	0.0
7	1.618091558092723E-4	0.0	0.0	6.023184015844352	0.0
8	1.618091558092723E-4	0.0	0.0	7.213128547665741	0.0
9	1.618091558092723E-4	0.0	0.0	7.8924033837530665	0.0
10	1.618091558092723E-4	0.0	0.0	9.137039410237989	0.0
11	1.618091558092723E-4	0.0	0.0	11.084412600402581	0.0
12	1.618091558092723E-4	0.0	0.0	12.851044963528217	0.0
13	1.618091558092723E-4	0.0	0.0	13.42368756593723	0.0
14	1.618091558092723E-4	0.0	0.0	13.626758056477868	0.0
15	1.618091558092723E-4	0.0	0.0	13.952156268810315	0.0
16	1.618091558092723E-4	0.0	0.0	14.689520591833169	0.0
17	1.618091558092723E-4	0.0	0.0	15.775421836469194	0.0
18	1.618091558092723E-4	0.0	0.0	17.04174028983256	0.0
19	1.618091558092723E-4	0.0	0.0	17.814055390510216	0.0
20	1.618091558092723E-4	0.0	0.0	18.601580551833944	0.0
21	1.618091558092723E-4	0.0	0.0	19.611916920707042	0.0
22	1.618091558092723E-4	0.0	0.0	20.58600803867886	0.0
23	1.618091558092723E-4	0.0	0.0	21.618835880209446	0.0
24	1.618091558092723E-4	0.0	0.0	22.404257522507653	0.0
25	1.618091558092723E-4	0.0	0.0	23.085312259308882	0.0
26	1.618091558092723E-4	0.0	0.0	23.66620712866417	0.0
27	1.618091558092723E-4	0.0	0.0	24.249043707889168	0.0
28	1.618091558092723E-4	0.0	0.0	24.868287347171254	0.0
29	1.618091558092723E-4	0.0	0.0	25.52911593949632	0.0
30	1.618091558092723E-4	0.0	0.0	26.301431040173977	0.0
31	1.618091558092723E-4	0.0	0.0	26.97471893749636	0.0
32	1.618091558092723E-4	0.0	0.0	27.560953508993354	0.0
33	1.618091558092723E-4	0.0	0.0	28.167899652433935	0.0
34	1.618091558092723E-4	0.0	0.0	28.76918247542119	0.0
35	3.236183116185446E-4	0.0	0.0	29.44311760936681	0.0
36	3.236183116185446E-4	0.0	0.0	30.04488585982149	0.0
37	3.236183116185446E-4	0.0	0.0	30.697947612667715	0.0
38	3.236183116185446E-4	0.0	0.0	31.30181938214792	0.0
39	3.236183116185446E-4	0.0	0.0	31.89533536565633	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.0307514E-4	45.000004	12
CTCCGTA	20	7.0307514E-4	45.000004	16
CTATACG	20	7.0307514E-4	45.000004	1
ATTCGAT	20	7.0307514E-4	45.000004	37
CGACATA	20	7.0307514E-4	45.000004	2
TGCGGTA	20	7.0307514E-4	45.000004	4
CGCGAGT	20	7.0307514E-4	45.000004	17
CGACAAG	20	7.0307514E-4	45.000004	22
ACGATAC	20	7.0307514E-4	45.000004	45
ACGGCTT	20	7.0307514E-4	45.000004	19
GTTCGAC	20	7.0307514E-4	45.000004	37
TAATTCG	20	7.0307514E-4	45.000004	24
CGTACTT	20	7.0307514E-4	45.000004	15
AACCTCG	20	7.0307514E-4	45.000004	38
TATACGG	20	7.0307514E-4	45.000004	2
AATACGG	20	7.0307514E-4	45.000004	27
TCGGTAC	20	7.0307514E-4	45.000004	36
TACGTAC	20	7.0307514E-4	45.000004	13
CGGGTCG	25	3.888705E-5	45.0	6
AACCGCG	25	3.888705E-5	45.0	26
>>END_MODULE