FastQCFastQC Report
Sat 14 Jan 2017
SRR2933140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264573
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148700.6566359309238431No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT34080.1504919470469709TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC32740.144574716734678TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC25170.11114678131373994TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC22830.10081370748481061TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG1200.041.2499961
AACGTAT556.184564E-1140.90909231
CGTTTTT75050.040.8927421
ACGTATA451.9297659E-840.032
TACGAAT2600.038.94230712
TTTGGGA103500.038.1521724
AACACGT17050.037.873941
TCGTTTG7850.037.8343961
GTATGCG301.1402719E-437.4999961
CGTCGAT301.1402719E-437.49999640
TGCGTAT301.1402719E-437.49999620
CGAATAT2650.037.3584914
TCAAGCG19350.037.3255817
TGGGAAC29750.037.2857136
CGAGACA19200.037.2656222
ACGACCA18400.037.17391228
TTGGGAT47700.037.02835
AAACACG17850.036.8067240
ACACGCG16700.036.78143736
GTCAAGC19900.036.7462316
Produced by FastQC (version 0.11.5)