##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933140.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2264573
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.272971107577455	33.0	31.0	34.0	30.0	34.0
2	32.464697759798426	34.0	31.0	34.0	31.0	34.0
3	32.5230337021593	34.0	31.0	34.0	31.0	34.0
4	35.99821909030974	37.0	35.0	37.0	35.0	37.0
5	35.23901238776582	37.0	35.0	37.0	35.0	37.0
6	34.91574923837739	37.0	35.0	37.0	32.0	37.0
7	35.68344098423853	37.0	35.0	37.0	35.0	37.0
8	35.964886978693116	37.0	35.0	37.0	35.0	37.0
9	37.84162577227583	39.0	38.0	39.0	35.0	39.0
10	37.38103607170093	39.0	37.0	39.0	35.0	39.0
11	37.27854831793897	39.0	37.0	39.0	34.0	39.0
12	37.03815112164633	39.0	37.0	39.0	33.0	39.0
13	36.93981646871176	39.0	37.0	39.0	33.0	39.0
14	37.93994055391458	40.0	37.0	41.0	33.0	41.0
15	38.07642853641724	40.0	37.0	41.0	33.0	41.0
16	38.13672997072737	40.0	37.0	41.0	33.0	41.0
17	38.09204295909207	40.0	37.0	41.0	33.0	41.0
18	38.03835689995421	40.0	37.0	41.0	33.0	41.0
19	38.00564698068907	40.0	37.0	41.0	33.0	41.0
20	37.875651612908925	40.0	36.0	41.0	33.0	41.0
21	37.76349227867682	40.0	36.0	41.0	33.0	41.0
22	37.831490086652096	40.0	36.0	41.0	33.0	41.0
23	37.7659426302442	40.0	35.0	41.0	33.0	41.0
24	37.70013817174364	40.0	35.0	41.0	33.0	41.0
25	37.5730002963031	39.0	35.0	41.0	33.0	41.0
26	37.537391375769296	39.0	35.0	41.0	33.0	41.0
27	37.49557775351026	39.0	35.0	41.0	33.0	41.0
28	37.379705578049375	39.0	35.0	41.0	33.0	41.0
29	37.293968885083416	39.0	35.0	41.0	33.0	41.0
30	37.178966189210946	39.0	35.0	41.0	32.0	41.0
31	37.03645587932029	39.0	35.0	41.0	32.0	41.0
32	36.95842483329087	39.0	35.0	41.0	32.0	41.0
33	36.789744909967574	39.0	35.0	41.0	31.0	41.0
34	36.55987994204647	39.0	35.0	41.0	31.0	41.0
35	36.39036630746723	39.0	35.0	41.0	30.0	41.0
36	36.24364681553653	39.0	35.0	41.0	30.0	41.0
37	36.20319018199016	39.0	35.0	41.0	30.0	41.0
38	36.064689899596964	38.0	35.0	40.0	30.0	41.0
39	35.99987900588764	38.0	35.0	40.0	30.0	41.0
40	35.868973974343064	38.0	35.0	40.0	29.0	41.0
41	35.72722054003117	38.0	35.0	40.0	29.0	41.0
42	35.658663686266685	38.0	35.0	40.0	29.0	41.0
43	35.54551167041204	38.0	35.0	40.0	28.0	41.0
44	35.46414312985274	38.0	34.0	40.0	28.0	41.0
45	35.45816584406862	38.0	34.0	40.0	29.0	41.0
46	35.29037527162957	37.0	34.0	40.0	28.0	41.0
47	35.18128759814764	37.0	34.0	40.0	28.0	41.0
48	35.074232537436416	37.0	34.0	40.0	27.0	41.0
49	35.108026104700535	37.0	34.0	40.0	28.0	41.0
50	34.924777872031505	37.0	34.0	40.0	27.0	41.0
51	33.816318573081986	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	27.0
10	57.0
11	50.0
12	36.0
13	48.0
14	58.0
15	79.0
16	163.0
17	324.0
18	617.0
19	1084.0
20	1862.0
21	3130.0
22	4679.0
23	7078.0
24	10791.0
25	16332.0
26	22652.0
27	27025.0
28	30662.0
29	34224.0
30	40786.0
31	51439.0
32	67539.0
33	97491.0
34	164939.0
35	184381.0
36	205509.0
37	294885.0
38	452440.0
39	543970.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.023116057640888	4.2587719627497105	53.16759495057125	18.550517029038147
2	21.943209602869945	4.540679412851783	51.850392987993764	21.66571799628451
3	20.863182595570997	4.645953122288396	52.1185671647591	22.37229711738151
4	18.5083015650191	4.874958767061163	52.058335059192174	24.55840460872756
5	17.85758286440755	7.565576380182931	49.8221077439323	24.75473301147722
6	22.245694883759544	7.4667498022806065	52.47099563582185	17.816559678137995
7	83.33478320195464	2.164337382809033	10.349633242116726	4.151246173119612
8	85.94600394864727	2.5444090342859336	8.147363763499786	3.362223253567008
9	80.5598229776651	4.5890770577941185	10.057348559750558	4.7937514047902186
10	41.88467318121341	26.013601681199944	16.492822267155884	15.60890287043076
11	32.74511353796058	23.346520514021847	24.51137587527538	19.39699007274219
12	28.08714932130693	20.38344535592361	30.538163265216	20.991242057553457
13	25.855823592350525	20.323301567227023	32.93495065074078	20.885924189681678
14	22.2471962705552	21.63772154838903	34.50831569571836	21.60676648533741
15	21.100843293636373	20.40159447277699	35.720155631988895	22.77740660159774
16	25.178653989074316	19.915630893771144	32.287499674331535	22.618215442822994
17	24.805382736613037	19.916072478122807	30.600117549754412	24.67842723550974
18	26.05144546013752	19.79181064156466	30.603252798651226	23.5534910996466
19	26.061646058660948	22.647183376292134	28.56662161034332	22.724548954703604
20	27.438196958102033	24.290407065702894	27.71727826835346	20.55411770784161
21	27.116149490433738	23.503415434167945	28.61016182741735	20.770273247980967
22	24.92597942305238	21.281672085642636	29.50825608183088	24.284092409474102
23	24.200191382658012	23.070353660491406	28.828083704963365	23.90137125188722
24	23.846261524799598	22.964020148610796	29.444049717098984	23.745668609490618
25	25.548966626379453	23.138843393434435	26.98115715413016	24.33103282605595
26	23.178674301954498	23.071457621370563	27.900138348377375	25.849729728297564
27	23.04995246344454	21.650085910235617	29.748654602876567	25.55130702344327
28	21.38827054813424	24.153162649205832	28.829055190537023	25.6295116121229
29	23.544791887918826	23.24217413172373	28.346535969474157	24.866498010883287
30	25.445856680265994	21.45411077496729	29.856401184682497	23.24363136008422
31	25.131625255622144	23.67788541151025	27.8625595200508	23.32792981281681
32	26.20785463749678	23.10104377293203	27.20031546786083	23.49078612171036
33	25.982823251888988	22.783058881299034	26.68087979499888	24.553238071813098
34	23.9191229428241	23.362461709116904	29.535281044152693	23.1831343039063
35	23.917135813241615	24.091429156843255	28.43538274102888	23.55605228888625
36	25.227846485849646	25.760794639872508	26.28588259243575	22.7254762818421
37	25.022642237631555	26.123291234153196	25.9808361223065	22.87323040590875
38	25.169822302041045	24.81213897719349	27.71255331579066	22.3054854049748
39	25.59520050799864	22.78018858301322	27.188216056625247	24.4363948523629
40	26.505217539907083	21.86323867678366	29.2085086239216	22.423035159387663
41	23.181765392416143	22.852652575121226	28.735350991114	25.230231041348635
42	23.585064380790552	23.048539393519217	28.87272788291656	24.49366834277367
43	23.71237314937518	22.100192839886372	29.036864786429938	25.150569224308512
44	24.265104282352567	22.676195468196433	28.189729366198396	24.868970883252604
45	23.763067032946168	21.886466013681165	27.56471087485367	26.785756078518997
46	23.382907064598935	22.611415043807376	28.479452859324915	25.52622503226878
47	21.794572310099962	23.973570293384228	30.364046555355028	23.867810841160782
48	22.592780184167168	24.233266050597617	29.023617255880023	24.150336509355185
49	24.375765320879477	23.035998397931973	28.804149833103192	23.784086448085358
50	23.00954749526732	22.772725807470106	28.486871476432864	25.730855220829714
51	22.31109352624093	22.703617856434747	28.00042215464019	26.98486646268414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	522.0
1	1619.5
2	2717.0
3	13969.5
4	25222.0
5	16513.5
6	7805.0
7	7566.5
8	7328.0
9	7313.5
10	7299.0
11	7187.5
12	7076.0
13	6914.5
14	6753.0
15	6455.0
16	6157.0
17	6010.5
18	5864.0
19	5748.5
20	5633.0
21	6129.0
22	6625.0
23	7210.5
24	7796.0
25	8915.0
26	12226.5
27	14419.0
28	17331.0
29	20243.0
30	23494.5
31	26746.0
32	31149.0
33	35552.0
34	41260.5
35	46969.0
36	49683.0
37	52397.0
38	57522.0
39	62647.0
40	68697.5
41	74748.0
42	82250.0
43	89752.0
44	102673.0
45	115594.0
46	135478.5
47	155363.0
48	176128.5
49	196894.0
50	198729.0
51	200564.0
52	188697.5
53	176831.0
54	167505.5
55	158180.0
56	151596.0
57	145012.0
58	137291.5
59	129571.0
60	121939.0
61	114307.0
62	104263.5
63	94220.0
64	83935.0
65	73650.0
66	64542.0
67	55434.0
68	47681.5
69	39929.0
70	34232.5
71	28536.0
72	24474.0
73	20412.0
74	17151.5
75	11202.0
76	8513.0
77	6243.0
78	3973.0
79	2962.5
80	1952.0
81	1402.0
82	852.0
83	633.0
84	414.0
85	261.5
86	109.0
87	67.5
88	26.0
89	21.0
90	16.0
91	12.0
92	8.0
93	10.5
94	13.0
95	8.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2264573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.22496039628834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9746225716066	20.139561698423673
2	9.85696303780179	5.367108566636635
3	3.5711074987240234	2.9166978067094957
4	1.827483393762981	1.9901265208028707
5	1.0784121233214665	1.4679863674152072
6	0.7897841100445219	1.2901104670547963
7	0.546764091658169	1.0419941519054352
8	0.43174397864908076	0.9403370176045746
9	0.381540943336544	0.9348693364709911
>10	6.886829559399857	47.885565494450944
>50	0.5675346781745915	9.408603585209418
>100	0.08056932682744292	3.9628094179638724
>500	0.003986812015793601	0.7999004650468233
>1k	0.0024917575098710003	1.1818284023635206
>5k	0.0	0.0
>10k+	1.6611716732473336E-4	0.672500701941893
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14870	0.6566359309238431	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	3408	0.1504919470469709	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	3274	0.144574716734678	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2517	0.11114678131373994	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2283	0.10081370748481061	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.091090461645529E-4	0.0	0.0	0.16131076366273023	0.0
2	3.091090461645529E-4	0.0	0.0	0.5565287584016942	0.0
3	3.091090461645529E-4	0.0	0.0	0.8430286857610684	0.0
4	3.091090461645529E-4	0.0	0.0	1.1956337905644905	0.0
5	3.091090461645529E-4	0.0	0.0	1.861896260354601	0.0
6	3.974259164972823E-4	0.0	0.0	2.6003136132065516	0.0
7	3.974259164972823E-4	0.0	0.0	3.2093467510210534	0.0
8	3.974259164972823E-4	0.0	0.0	4.083462975139242	0.0
9	3.974259164972823E-4	0.0	0.0	4.662689169216448	0.0
10	4.857427868300117E-4	0.0	0.0	5.525015091145218	0.0
11	4.857427868300117E-4	0.0	0.0	6.755401570185637	0.0
12	4.857427868300117E-4	0.0	0.0	7.872035920237502	0.0
13	4.857427868300117E-4	0.0	0.0	8.248663213771426	0.0
14	4.857427868300117E-4	0.0	0.0	8.433068839026165	0.0
15	4.857427868300117E-4	0.0	0.0	8.632664965978133	0.0
16	4.857427868300117E-4	0.0	0.0	9.043294254590158	0.0
17	4.857427868300117E-4	0.0	0.0	9.567278246274242	0.0
18	4.857427868300117E-4	0.0	0.0	10.140101467252325	0.0
19	5.299012219963764E-4	0.0	0.0	10.552143825789674	0.0
20	5.74059657162741E-4	0.0	0.0	11.012142244917696	0.0
21	6.182180923291058E-4	0.0	0.0	11.629079742626976	0.0
22	6.623765274954705E-4	0.0	0.0	12.308943010448328	0.0
23	6.623765274954705E-4	0.0	0.0	12.947341507648462	0.0
24	6.623765274954705E-4	0.0	0.0	13.467395398602738	0.0
25	6.623765274954705E-4	0.0	0.0	13.92059341871514	0.0
26	7.065349626618351E-4	0.0	0.0	14.322567654034557	0.0
27	7.065349626618351E-4	0.0	0.0	14.755055368053933	0.0
28	7.065349626618351E-4	0.0	0.0	15.185158526574325	0.0
29	7.065349626618351E-4	0.0	0.0	15.684811220481741	0.0
30	7.065349626618351E-4	0.0	0.0	16.313980604732105	0.0
31	7.065349626618351E-4	0.0	0.0	16.783605562726393	0.0
32	7.065349626618351E-4	0.0	0.0	17.26312201019795	0.0
33	7.065349626618351E-4	0.0	0.0	17.73963568407819	0.0
34	7.065349626618351E-4	0.0	0.0	18.238714318328444	0.0
35	7.065349626618351E-4	0.0	0.0	18.841079532432826	0.0
36	7.065349626618351E-4	0.0	0.0	19.337199551526933	0.0
37	7.065349626618351E-4	0.0	0.0	19.834688482111197	0.0
38	7.065349626618351E-4	0.0	0.0	20.433830130448435	0.0
39	7.065349626618351E-4	0.0	0.0	21.41255768747574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	120	0.0	41.249996	1
AACGTAT	55	6.184564E-11	40.909092	31
CGTTTTT	7505	0.0	40.892742	1
ACGTATA	45	1.9297659E-8	40.0	32
TACGAAT	260	0.0	38.942307	12
TTTGGGA	10350	0.0	38.152172	4
AACACGT	1705	0.0	37.8739	41
TCGTTTG	785	0.0	37.834396	1
GTATGCG	30	1.1402719E-4	37.499996	1
CGTCGAT	30	1.1402719E-4	37.499996	40
TGCGTAT	30	1.1402719E-4	37.499996	20
CGAATAT	265	0.0	37.35849	14
TCAAGCG	1935	0.0	37.32558	17
TGGGAAC	2975	0.0	37.285713	6
CGAGACA	1920	0.0	37.26562	22
ACGACCA	1840	0.0	37.173912	28
TTGGGAT	4770	0.0	37.0283	5
AAACACG	1785	0.0	36.80672	40
ACACGCG	1670	0.0	36.781437	36
GTCAAGC	1990	0.0	36.74623	16
>>END_MODULE