SRR2933139.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1331365
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3173	0.23832682998276206	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	3054	0.22938863497237796	Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2516	0.1889789802195491	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2411	0.18109233756332788	TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2348	0.1763603519695951	TruSeq Adapter, Index 22 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGC	20	7.033691E-4	45.0	25
GTAACGA	20	7.033691E-4	45.0	30
TACGCCC	25	3.8911407E-5	44.999996	17
CATACGT	25	3.8911407E-5	44.999996	34
TATCGTG	30	2.1657615E-6	44.999996	1
TCGTTAG	45	1.9286745E-8	40.0	1
CTTTGCG	455	0.0	39.560444	1
TCTTACG	40	3.458772E-7	39.375	1
TTTGGGC	4285	0.0	39.171528	4
TTGGGCA	2110	0.0	38.815166	5
TTGGGAC	2455	0.0	38.217922	5
CGTTAGG	130	0.0	38.07692	2
TTTGGGA	5935	0.0	37.98652	4
CGAATAT	95	0.0	37.894737	14
CGACCAA	180	0.0	37.5	29
ATTAACG	30	1.1400191E-4	37.499996	10
ATATCCG	30	1.1400191E-4	37.499996	40
ATACGAG	30	1.1400191E-4	37.499996	24
CGTTTTT	1985	0.0	37.40554	1
TAGGGCG	205	0.0	37.317074	5