##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1331365
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.256861191333705	33.0	31.0	34.0	30.0	34.0
2	32.43998678048469	34.0	31.0	34.0	31.0	34.0
3	32.51591637154349	34.0	31.0	34.0	31.0	34.0
4	35.98094812466904	37.0	35.0	37.0	35.0	37.0
5	35.19825216976562	37.0	35.0	37.0	35.0	37.0
6	34.8891220664506	37.0	35.0	37.0	32.0	37.0
7	35.67786444739046	37.0	35.0	37.0	35.0	37.0
8	35.97010661989762	37.0	35.0	37.0	35.0	37.0
9	37.85725552346652	39.0	38.0	39.0	35.0	39.0
10	37.31833268863159	39.0	37.0	39.0	34.0	39.0
11	37.22762127590856	39.0	37.0	39.0	34.0	39.0
12	37.04273508767318	39.0	37.0	39.0	33.0	39.0
13	36.99202697982897	39.0	37.0	39.0	33.0	39.0
14	38.140591047533924	40.0	37.0	41.0	33.0	41.0
15	38.23420925140739	40.0	37.0	41.0	33.0	41.0
16	38.24737919353446	40.0	37.0	41.0	34.0	41.0
17	38.20518189977955	40.0	37.0	41.0	34.0	41.0
18	38.17837332361899	40.0	37.0	41.0	34.0	41.0
19	38.17525171534478	40.0	37.0	41.0	34.0	41.0
20	38.09541185174614	40.0	37.0	41.0	34.0	41.0
21	37.978660247189914	40.0	36.0	41.0	33.0	41.0
22	38.01624573276299	40.0	37.0	41.0	34.0	41.0
23	37.935623964878154	40.0	36.0	41.0	33.0	41.0
24	37.86706200027791	40.0	36.0	41.0	33.0	41.0
25	37.750556759416085	40.0	36.0	41.0	33.0	41.0
26	37.71441265167704	40.0	36.0	41.0	33.0	41.0
27	37.67045550994656	40.0	36.0	41.0	33.0	41.0
28	37.579189778911115	39.0	36.0	41.0	33.0	41.0
29	37.53040375854856	39.0	36.0	41.0	33.0	41.0
30	37.46251929410793	39.0	36.0	41.0	33.0	41.0
31	37.38508147652973	39.0	35.0	41.0	33.0	41.0
32	37.355472015562974	39.0	35.0	41.0	33.0	41.0
33	37.263322980550036	39.0	35.0	41.0	32.0	41.0
34	37.11833268863159	39.0	35.0	41.0	32.0	41.0
35	37.00652112681346	39.0	35.0	41.0	31.0	41.0
36	36.88344293262929	39.0	35.0	41.0	31.0	41.0
37	36.825552722206154	39.0	35.0	41.0	31.0	41.0
38	36.74075629147529	39.0	35.0	41.0	31.0	41.0
39	36.710084762630835	39.0	35.0	41.0	31.0	41.0
40	36.573661617963516	39.0	35.0	41.0	31.0	41.0
41	36.44796355619984	39.0	35.0	40.0	30.0	41.0
42	36.390230327521	39.0	35.0	40.0	30.0	41.0
43	36.26171861210111	38.0	35.0	40.0	30.0	41.0
44	36.18419742144341	38.0	35.0	40.0	30.0	41.0
45	36.18677522692875	38.0	35.0	40.0	30.0	41.0
46	35.96219293732372	38.0	35.0	40.0	30.0	41.0
47	35.86384124563887	38.0	35.0	40.0	30.0	41.0
48	35.77504065376512	38.0	35.0	40.0	29.0	41.0
49	35.818511828086216	38.0	35.0	40.0	29.0	41.0
50	35.64387076421567	37.0	34.0	40.0	29.0	41.0
51	34.51723531863914	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	28.0
10	35.0
11	35.0
12	29.0
13	28.0
14	34.0
15	34.0
16	58.0
17	145.0
18	263.0
19	449.0
20	857.0
21	1263.0
22	1990.0
23	3077.0
24	4370.0
25	6422.0
26	9280.0
27	11707.0
28	13724.0
29	16714.0
30	20831.0
31	27408.0
32	36243.0
33	51900.0
34	88043.0
35	112831.0
36	119138.0
37	175960.0
38	279559.0
39	348795.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.064024516192028	4.010395346129724	54.87465871492792	17.050921422750335
2	19.720587517322446	4.5354204143867385	54.76867725980479	20.97531480848603
3	21.0096404817612	4.5579536791188	52.25944801012494	22.172957828995056
4	18.398410653727566	4.841797703860324	51.8500185899434	24.909773052468704
5	17.377879094012535	7.963255756310253	50.38099995117793	24.277865198499285
6	21.92674435635607	7.676782850683321	52.59226433021749	17.804208462743123
7	85.57585635794842	1.9135999519290352	8.484525280445258	4.0260184096772855
8	88.20113192099838	2.2591851220364063	5.9004855918549755	3.6391973651102436
9	82.88140367217103	4.450169562817108	7.89535551858431	4.773071246427539
10	40.736311980561304	26.096975660318545	15.994336639464008	17.17237571965614
11	31.713467005667116	26.06490331351658	22.86878504392109	19.352844636895217
12	27.360566035610066	22.044818663552068	30.376343076466632	20.21827222437123
13	24.689097279859393	22.82454473416381	30.853146958197037	21.633211027779762
14	21.545181073559842	25.109117334465004	30.534827038415465	22.810874553559692
15	19.98910892204617	24.01204778554341	34.142102278488615	21.856741013921802
16	22.34939329184709	21.353948766867088	32.495145959222306	23.801511982063523
17	21.53323844325185	21.63043192512947	30.300631306966906	26.53569832465177
18	22.65486925073139	22.64795904954689	30.06365647286807	24.633515226853643
19	24.429514070146052	24.800787162047975	28.43735564627281	22.332343121533164
20	27.926751867444317	24.266448344368374	27.128323187104964	20.678476601082348
21	26.30923901409456	24.8327843979675	27.01220176285241	21.84577482508553
22	24.159190004243765	23.850859831826735	26.961501917205272	25.028448246724228
23	24.30325267676407	25.060595704408634	26.11703026593008	24.519121352897212
24	24.16144333071697	23.768913859084474	28.160872488010426	23.90877032218813
25	23.331242747105417	24.1060114994761	26.97802631134212	25.584719442076363
26	22.466641379336245	25.483469972546974	27.763235476371996	24.28665317174479
27	22.819887859452518	25.07441610677763	28.37486339208256	23.73083264168729
28	20.6907196749201	26.023517217292024	29.6889282803739	23.59683482741397
29	22.447337882549114	23.80391553030161	28.917839961242787	24.830906625906493
30	24.12366255684955	24.8109271311774	27.896181738291155	23.1692285736819
31	24.234075554036647	26.00226083756145	26.297747049081206	23.465916559320696
32	24.785013876735533	25.50412546521803	25.917836205698663	23.793024452347776
33	24.82910396472793	25.14779943892171	25.613486910050966	24.4096096862994
34	22.899204951309372	24.674751101313312	27.978878819857815	24.4471651275195
35	22.923165322807794	23.96517859490072	27.82016952526167	25.291486557029813
36	24.843750586803772	26.38104501770739	25.83866933560669	22.93653505988215
37	23.85709403506927	26.880231942404976	26.64971664419599	22.61295737832976
38	23.07421330739504	26.465169206040418	25.930004168653976	24.530613317910564
39	24.035782824394513	24.243915079636313	26.744356356070647	24.975945739898524
40	24.44228292016089	23.213468883439177	29.17697250566148	23.167275690738453
41	21.641548335730622	23.85664336977463	29.30248279021906	25.199325504275688
42	23.083602167700068	24.316246859426226	26.631539810645467	25.96861116222824
43	22.878925013050516	23.944748434876985	27.124717864747833	26.051608687324663
44	22.75762093790959	24.004611808181828	27.528138414334162	25.70962883957442
45	23.387275465405803	23.607951238015122	26.60284745355331	26.401925843025765
46	23.598111712415452	24.23415066491909	27.99164766987265	24.176089952792807
47	22.24596560672693	24.396915947167006	29.09915763145343	24.25796081465263
48	22.58726945653521	23.164121033675965	29.01323078194184	25.235378727846985
49	22.847303331543188	22.953960784608277	29.166156538590094	25.032579345258437
50	22.361561254802403	22.538071828536875	28.336857285567817	26.7635096310929
51	21.281917430606935	23.165623251324767	27.480893669279272	28.071565648789026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	216.0
1	1054.0
2	1892.0
3	4034.5
4	6177.0
5	4306.0
6	2435.0
7	2431.5
8	2428.0
9	2389.0
10	2350.0
11	2456.5
12	2563.0
13	2630.0
14	2697.0
15	2519.5
16	2342.0
17	2414.0
18	2486.0
19	2564.0
20	2642.0
21	3259.0
22	3876.0
23	4476.0
24	5076.0
25	6177.0
26	9066.0
27	10854.0
28	12622.0
29	14390.0
30	16906.5
31	19423.0
32	22774.5
33	26126.0
34	28738.5
35	31351.0
36	34277.5
37	37204.0
38	40952.5
39	44701.0
40	50693.5
41	56686.0
42	60023.0
43	63360.0
44	69529.0
45	75698.0
46	82325.5
47	88953.0
48	98742.5
49	108532.0
50	115978.0
51	123424.0
52	120662.0
53	117900.0
54	106519.0
55	95138.0
56	86559.0
57	77980.0
58	74368.5
59	70757.0
60	66484.5
61	62212.0
62	55999.5
63	49787.0
64	41625.5
65	33464.0
66	28993.5
67	24523.0
68	21155.0
69	17787.0
70	15506.0
71	13225.0
72	11478.0
73	9731.0
74	8049.5
75	4857.5
76	3347.0
77	2705.5
78	2064.0
79	1490.0
80	916.0
81	776.0
82	636.0
83	438.0
84	240.0
85	148.0
86	56.0
87	57.5
88	59.0
89	33.5
90	8.0
91	5.5
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1331365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.971936323232008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38887426840769	22.595505390528594
2	10.463477784248461	6.272213797380953
3	3.7251151147258814	3.349467390458196
4	1.7172298473062686	2.058748145432676
5	1.0206898823436352	1.5296026079685303
6	0.6559029750970995	1.1795209322297218
7	0.48219932384480646	1.0116713200567446
8	0.3770081037459481	0.9039730303052803
9	0.2843868652472399	0.7671262514718452
>10	4.955452502689772	39.46770625839019
>50	0.8550000106308134	15.70455923052716
>100	0.07147048230772518	3.783140706679321
>500	0.0019648242490143248	0.3828559292897939
>1k	0.001228015155633953	0.993909009281061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3173	0.23832682998276206	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	3054	0.22938863497237796	Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2516	0.1889789802195491	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2411	0.18109233756332788	TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2348	0.1763603519695951	TruSeq Adapter, Index 22 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.257761770814164E-4	0.0	0.0	0.2522974541166397	0.0
2	5.257761770814164E-4	0.0	0.0	0.8864586345592681	0.0
3	5.257761770814164E-4	0.0	0.0	1.2637405970564046	0.0
4	5.257761770814164E-4	0.0	0.0	1.7236445302377634	0.0
5	5.257761770814164E-4	0.0	0.0	2.4607827305059096	0.0
6	5.257761770814164E-4	0.0	0.0	2.9856575769980433	0.0
7	5.257761770814164E-4	0.0	0.0	3.4856707214024705	0.0
8	5.257761770814164E-4	0.0	0.0	4.280268746737371	0.0
9	5.257761770814164E-4	0.0	0.0	4.715235866948583	0.0
10	5.257761770814164E-4	0.0	0.0	5.5307898284843	0.0
11	5.257761770814164E-4	0.0	0.0	6.928378018049145	0.0
12	5.257761770814164E-4	0.0	0.0	8.244470900166371	0.0
13	5.257761770814164E-4	0.0	0.0	8.616645322657574	0.0
14	5.257761770814164E-4	0.0	0.0	8.743132048686874	0.0
15	5.257761770814164E-4	0.0	0.0	8.941349667446568	0.0
16	5.257761770814164E-4	0.0	0.0	9.451502780980423	0.0
17	5.257761770814164E-4	0.0	0.0	10.186087211245601	0.0
18	5.257761770814164E-4	0.0	0.0	11.029957975461274	0.0
19	5.257761770814164E-4	0.0	0.0	11.581947850514322	0.0
20	5.257761770814164E-4	0.0	0.0	12.154292774708663	0.0
21	5.257761770814164E-4	0.0	0.0	12.979010263902085	0.0
22	5.257761770814164E-4	0.0	0.0	13.838504091665321	0.0
23	5.257761770814164E-4	0.0	0.0	14.670357114690562	0.0
24	5.257761770814164E-4	0.0	0.0	15.302565412189745	0.0
25	5.257761770814164E-4	0.0	0.0	15.877388995504614	0.0
26	5.257761770814164E-4	0.0	0.0	16.346231123696356	0.0
27	5.257761770814164E-4	0.0	0.0	16.815749249830063	0.0
28	5.257761770814164E-4	0.0	0.0	17.32770502454248	0.0
29	5.257761770814164E-4	0.0	0.0	17.88074645194969	0.0
30	5.257761770814164E-4	0.0	0.0	18.479530406762983	0.0
31	5.257761770814164E-4	0.0	0.0	19.03399894093656	0.0
32	5.257761770814164E-4	0.0	0.0	19.57727595362654	0.0
33	5.257761770814164E-4	0.0	0.0	20.125360062792698	0.0
34	5.257761770814164E-4	0.0	0.0	20.659473547824977	0.0
35	5.257761770814164E-4	0.0	0.0	21.21747229347324	0.0
36	5.257761770814164E-4	0.0	0.0	21.775621260886382	0.0
37	5.257761770814164E-4	0.0	0.0	22.311912961509428	0.0
38	5.257761770814164E-4	0.0	0.0	22.864503723621997	0.0
39	5.257761770814164E-4	0.0	0.0	23.425431793685426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGC	20	7.033691E-4	45.0	25
GTAACGA	20	7.033691E-4	45.0	30
TACGCCC	25	3.8911407E-5	44.999996	17
CATACGT	25	3.8911407E-5	44.999996	34
TATCGTG	30	2.1657615E-6	44.999996	1
TCGTTAG	45	1.9286745E-8	40.0	1
CTTTGCG	455	0.0	39.560444	1
TCTTACG	40	3.458772E-7	39.375	1
TTTGGGC	4285	0.0	39.171528	4
TTGGGCA	2110	0.0	38.815166	5
TTGGGAC	2455	0.0	38.217922	5
CGTTAGG	130	0.0	38.07692	2
TTTGGGA	5935	0.0	37.98652	4
CGAATAT	95	0.0	37.894737	14
CGACCAA	180	0.0	37.5	29
ATTAACG	30	1.1400191E-4	37.499996	10
ATATCCG	30	1.1400191E-4	37.499996	40
ATACGAG	30	1.1400191E-4	37.499996	24
CGTTTTT	1985	0.0	37.40554	1
TAGGGCG	205	0.0	37.317074	5
>>END_MODULE