##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933138.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1152524
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27407932502924	33.0	31.0	34.0	30.0	34.0
2	32.45491547247606	34.0	31.0	34.0	31.0	34.0
3	32.532404531272235	34.0	31.0	34.0	31.0	34.0
4	35.985195102227806	37.0	35.0	37.0	35.0	37.0
5	35.196216304389324	37.0	35.0	37.0	35.0	37.0
6	34.86724094248797	37.0	35.0	37.0	32.0	37.0
7	35.682219198906054	37.0	35.0	37.0	35.0	37.0
8	35.97528641485991	37.0	35.0	37.0	35.0	37.0
9	37.86543707549691	39.0	38.0	39.0	35.0	39.0
10	37.36382929986708	39.0	37.0	39.0	34.0	39.0
11	37.26194074917312	39.0	37.0	39.0	34.0	39.0
12	37.0669027282729	39.0	37.0	39.0	33.0	39.0
13	36.993751973928525	39.0	37.0	39.0	33.0	39.0
14	38.15887478265095	40.0	37.0	41.0	33.0	41.0
15	38.268684209612985	40.0	37.0	41.0	33.0	41.0
16	38.26139412281219	40.0	37.0	41.0	34.0	41.0
17	38.20658311670733	40.0	37.0	41.0	34.0	41.0
18	38.177163338897934	40.0	37.0	41.0	34.0	41.0
19	38.173520898480206	40.0	37.0	41.0	34.0	41.0
20	38.09762139443517	40.0	37.0	41.0	34.0	41.0
21	37.96618031381559	40.0	36.0	41.0	33.0	41.0
22	37.9963376033818	40.0	36.0	41.0	33.0	41.0
23	37.91763728998268	40.0	36.0	41.0	33.0	41.0
24	37.859845001058545	40.0	36.0	41.0	33.0	41.0
25	37.744612693531764	40.0	36.0	41.0	33.0	41.0
26	37.70257799403743	40.0	36.0	41.0	33.0	41.0
27	37.650738726482054	40.0	36.0	41.0	33.0	41.0
28	37.56177138176732	40.0	36.0	41.0	33.0	41.0
29	37.50943060621731	39.0	36.0	41.0	33.0	41.0
30	37.41357229871135	39.0	35.0	41.0	33.0	41.0
31	37.34396593910409	39.0	35.0	41.0	32.0	41.0
32	37.27821633215447	39.0	35.0	41.0	32.0	41.0
33	37.14840992465233	39.0	35.0	41.0	32.0	41.0
34	37.0521889348942	39.0	35.0	41.0	32.0	41.0
35	36.90597505995537	39.0	35.0	41.0	31.0	41.0
36	36.76728727557951	39.0	35.0	41.0	31.0	41.0
37	36.7213637199746	39.0	35.0	41.0	31.0	41.0
38	36.61132956884195	39.0	35.0	41.0	31.0	41.0
39	36.58974737185516	39.0	35.0	41.0	31.0	41.0
40	36.460028598102944	39.0	35.0	41.0	30.0	41.0
41	36.34624962256751	38.0	35.0	40.0	30.0	41.0
42	36.256734783830964	38.0	35.0	40.0	30.0	41.0
43	36.14166906719513	38.0	35.0	40.0	30.0	41.0
44	36.07538411347616	38.0	35.0	40.0	30.0	41.0
45	36.061124106743115	38.0	35.0	40.0	30.0	41.0
46	35.82945864901729	38.0	35.0	40.0	29.0	41.0
47	35.73899025096224	38.0	35.0	40.0	29.0	41.0
48	35.647609941311416	38.0	34.0	40.0	29.0	41.0
49	35.684429131193795	37.0	34.0	40.0	29.0	41.0
50	35.500732305791466	37.0	34.0	40.0	29.0	41.0
51	34.43170120535451	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	19.0
10	18.0
11	23.0
12	14.0
13	21.0
14	26.0
15	29.0
16	63.0
17	127.0
18	228.0
19	419.0
20	766.0
21	1199.0
22	1849.0
23	2793.0
24	4040.0
25	5923.0
26	8334.0
27	10446.0
28	12428.0
29	15158.0
30	18692.0
31	24096.0
32	31530.0
33	44608.0
34	76645.0
35	100590.0
36	103814.0
37	150939.0
38	237189.0
39	300385.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.496062554879554	4.3875875903668815	53.55662875567017	17.559721099083404
2	20.00140561064238	4.57994800975945	53.83801118241356	21.580635197184613
3	21.556080393987457	4.516001402139999	51.92828956273362	21.999628641138926
4	18.69679069589874	4.710444207669428	52.154401990761144	24.438363105670685
5	18.050296566492325	7.606522727509361	50.042081553182406	24.301099152815905
6	21.761368960646372	7.730858533097792	53.186658152021124	17.321114354234705
7	85.4370928501272	1.8622605689773055	8.548542156172019	4.152104424723476
8	87.62906455744088	2.4912279483984716	6.193710499737967	3.685996994422676
9	82.53225095529464	4.710444207669428	8.004518777916989	4.752786059118942
10	40.95584994325498	28.66734228527996	14.811578761049663	15.565229010415402
11	31.930961958275923	25.268974876011256	23.023381725673392	19.776681440039425
12	27.699293029906535	22.612023697554235	29.336829428280886	20.35185384425834
13	25.00546626360926	23.680895148387364	30.199457885475706	21.11418070252767
14	21.347755014212286	25.244767137170243	30.318674491811016	23.08880335680645
15	19.934248657728602	23.149713151309648	33.77951348518556	23.136524705776193
16	22.060104605196944	21.945920432025712	32.57832374857269	23.41565121420465
17	22.01559360152153	21.226542787829146	30.228264227035616	26.529599383613707
18	23.650266718957695	21.88197382440626	29.68129080175337	24.786468654882675
19	25.126071127369148	23.247325001475026	27.86276034165015	23.763843529505678
20	28.649208172671457	23.121687704551057	26.38608827234834	21.843015850429147
21	27.001867206236053	24.72260881335226	26.635627544415563	21.639896435996125
22	24.889026172123096	23.908048769483326	26.186092437120617	25.01683262127296
23	24.881043691931794	25.40675942540025	25.603024318799434	24.10917256386852
24	23.858592098732867	23.099475585757865	28.704217873120214	24.337714442389053
25	24.0056606196487	24.678184575765886	26.474849981432058	24.841304823153358
26	23.435867712950014	26.102449927290017	25.934123714560393	24.52755864519958
27	23.667186106319697	25.190538331522816	26.93306169763059	24.209213864526898
28	20.78230041196539	26.220191510111718	29.315311438199988	23.682196639722903
29	22.07086360023739	24.91557659536808	28.565478896751824	24.448080907642705
30	23.85243170641132	25.295525299256237	27.596301682220936	23.255741312111507
31	25.103859008575963	26.539577483852835	25.61074650072363	22.74581700684758
32	25.673738681363684	26.22964901381663	25.619770173983365	22.47684213083632
33	25.069239339050643	25.965706570969456	25.453613113479634	23.51144097650027
34	23.63837976475978	24.73128542225585	27.80115641843467	23.829178394549704
35	23.850609618541565	25.15071269665534	26.78278283142043	24.215894853382665
36	24.954968399790374	27.544675859244578	25.476519360985105	22.02383637997994
37	23.66770670285391	27.36984218983726	26.876403441490154	22.086047665818672
38	22.2589724812672	27.364896522762216	27.36749950543329	23.00863149053729
39	23.867529005903563	25.981758297441093	26.74391162353235	23.406801073122992
40	25.124075507321326	24.644432567130924	28.043580871200945	22.187911054346806
41	22.569248015659543	24.47810197444912	28.14605162235233	24.806598387539
42	23.46337256317439	24.771891951924648	27.32940919234654	24.43532629255443
43	24.102665107190827	23.591959907125577	27.300776382964692	25.004598602718904
44	23.430488215429786	25.16902034144191	27.467193741735528	23.93329770139277
45	23.9761601493765	24.539705897664604	25.68631976427389	25.79781418868501
46	24.013816632018077	25.2930090826742	27.215658849620482	23.47751543568724
47	22.3155439713186	25.333181781897817	29.299953840440633	23.05132040634295
48	22.578445221097347	24.30630511815806	29.056401428516892	24.058848232227703
49	22.788072092208058	24.109866692580805	29.432532424487473	23.669528790723664
50	22.629810745806594	24.815622060798734	28.04054405808469	24.51402313530998
51	21.86106319694861	24.297281444898328	27.062516702472138	26.779138655680924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	209.0
1	774.0
2	1339.0
3	3271.5
4	5204.0
5	3689.5
6	2175.0
7	2078.0
8	1981.0
9	2053.0
10	2125.0
11	2151.0
12	2177.0
13	2188.5
14	2200.0
15	2339.5
16	2479.0
17	2270.5
18	2062.0
19	2168.5
20	2275.0
21	2650.0
22	3025.0
23	3358.5
24	3692.0
25	4121.5
26	6238.0
27	7925.0
28	9659.0
29	11393.0
30	13071.5
31	14750.0
32	17266.5
33	19783.0
34	23284.5
35	26786.0
36	28738.0
37	30690.0
38	33971.0
39	37252.0
40	45181.0
41	53110.0
42	59505.5
43	65901.0
44	77191.5
45	88482.0
46	97904.0
47	107326.0
48	104947.0
49	102568.0
50	94523.5
51	86479.0
52	79151.5
53	71824.0
54	67871.5
55	63919.0
56	64384.0
57	64849.0
58	61633.5
59	58418.0
60	56870.0
61	55322.0
62	49808.5
63	44295.0
64	38035.0
65	31775.0
66	28033.5
67	24292.0
68	20108.0
69	15924.0
70	14025.5
71	12127.0
72	10350.0
73	8573.0
74	7239.0
75	4787.5
76	3670.0
77	2793.5
78	1917.0
79	1439.0
80	961.0
81	760.0
82	559.0
83	360.0
84	161.0
85	101.5
86	42.0
87	38.5
88	35.0
89	23.5
90	12.0
91	8.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1152524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.663256331262218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83939706331975	22.496434750976285
2	10.62607561256712	6.304080093819053
3	3.5367999736229483	3.147390126299369
4	1.6293222697628549	1.933240165368381
5	0.9183895654400362	1.3621212545802155
6	0.5824118558090288	1.0365739301537609
7	0.4266814305195705	0.885973245170417
8	0.3269982164616011	0.7759865531812833
9	0.24959852380186434	0.6663514492295419
>10	4.88899460344042	39.69605367379607
>50	0.8995387196104491	16.25842887469371
>100	0.07123323838781524	3.7068108487334968
>500	0.0028493295354024978	0.542029276338587
>1k	0.0017095977212414986	1.1885257576599424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2689	0.233314013417508	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2648	0.22975660376703652	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2643	0.22932277332185705	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2461	0.21353134511732508	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2399	0.20815184759709993	TruSeq Adapter, Index 15 (95% over 22bp)
TGTTTTGGGACAACCATCCGTAACAAGATCTGACTCCCTCTTCTGGAGTGT	1222	0.1060281608018575	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.470643561435597E-4	0.0	0.0	0.2488451433549323	0.0
2	3.470643561435597E-4	0.0	0.0	0.9751640746743668	0.0
3	3.470643561435597E-4	0.0	0.0	1.4072591980730986	0.0
4	3.470643561435597E-4	0.0	0.0	1.8548854514092548	0.0
5	3.470643561435597E-4	0.0	0.0	2.6040238641451285	0.0
6	3.470643561435597E-4	0.0	0.0	3.1116054850050845	0.0
7	3.470643561435597E-4	0.0	0.0	3.579968833620818	0.0
8	3.470643561435597E-4	0.0	0.0	4.340213305753286	0.0
9	3.470643561435597E-4	0.0	0.0	4.748360988578112	0.0
10	3.470643561435597E-4	0.0	0.0	5.522488034956322	0.0
11	3.470643561435597E-4	0.0	0.0	6.73174701784952	0.0
12	3.470643561435597E-4	0.0	0.0	7.8642180119459555	0.0
13	3.470643561435597E-4	0.0	0.0	8.22004574308214	0.0
14	3.470643561435597E-4	0.0	0.0	8.354619947176804	0.0
15	3.470643561435597E-4	0.0	0.0	8.556958466808501	0.0
16	3.470643561435597E-4	0.0	0.0	9.043629460210807	0.0
17	3.470643561435597E-4	0.0	0.0	9.711468047520052	0.0
18	4.3383044517944963E-4	0.0	0.0	10.467808045645905	0.0
19	4.3383044517944963E-4	0.0	0.0	10.950140734596417	0.0
20	4.3383044517944963E-4	0.0	0.0	11.46639896435996	0.0
21	4.3383044517944963E-4	0.0	0.0	12.183260391974484	0.0
22	4.3383044517944963E-4	0.0	0.0	12.953916794791258	0.0
23	4.3383044517944963E-4	0.0	0.0	13.679194533042262	0.0
24	4.3383044517944963E-4	0.0	0.0	14.257664048644541	0.0
25	4.3383044517944963E-4	0.0	0.0	14.761948558121132	0.0
26	5.205965342153395E-4	0.0	0.0	15.226754497086395	0.0
27	5.205965342153395E-4	0.0	0.0	15.672211598196654	0.0
28	5.205965342153395E-4	0.0	0.0	16.142049970326	0.0
29	5.205965342153395E-4	0.0	0.0	16.649978655542096	0.0
30	5.205965342153395E-4	0.0	0.0	17.234608563465923	0.0
31	5.205965342153395E-4	0.0	0.0	17.750346196695254	0.0
32	5.205965342153395E-4	0.0	0.0	18.240314301480925	0.0
33	5.205965342153395E-4	0.0	0.0	18.74997830847774	0.0
34	5.205965342153395E-4	0.0	0.0	19.261030572899134	0.0
35	5.205965342153395E-4	0.0	0.0	19.82561751425567	0.0
36	5.205965342153395E-4	0.0	0.0	20.350292054655696	0.0
37	5.205965342153395E-4	0.0	0.0	20.87496659505572	0.0
38	5.205965342153395E-4	0.0	0.0	21.360856693656704	0.0
39	5.205965342153395E-4	0.0	0.0	21.869045677139912	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	30	2.1655069E-6	45.000004	12
CGTGCAA	20	7.0332934E-4	45.000004	16
ATTACGA	20	7.0332934E-4	45.000004	13
GCGATAG	35	1.211938E-7	45.000004	9
GACGCAT	35	1.211938E-7	45.000004	11
TCGAATC	25	3.8908132E-5	45.0	34
CGTAACA	300	0.0	42.75	19
CGCAATC	95	0.0	42.63158	18
TCCGTAA	315	0.0	42.142857	17
CTTTACG	60	3.6379788E-12	41.250004	1
CGATAGG	55	6.184564E-11	40.909092	2
AGACACG	665	0.0	40.601505	24
AACACGT	625	0.0	40.32	41
TTTACGG	230	0.0	40.1087	2
TACGGGA	215	0.0	39.76744	4
CCGTAAC	335	0.0	39.626865	18
GTCAAGC	695	0.0	39.496403	16
ACACGAC	685	0.0	39.416058	26
CGAACGT	40	3.4582263E-7	39.375004	18
CAACGAC	115	0.0	39.130436	10
>>END_MODULE