FastQCFastQC Report
Sat 14 Jan 2017
SRR2933136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC30000.2698329464228696Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28070.25247369353633164No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT26840.24141054273299398Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC22060.19841715993628345Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC20220.18186740588901412Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.033186E-445.00000416
ACGATTA207.033186E-445.00000411
GCGATAT207.033186E-445.0000049
CCGATCA351.2118835E-745.036
CGTTGAA253.8907237E-545.018
CATCCGC253.8907237E-545.041
TACGAAA2050.042.8048820
ATACGAA2300.041.0869619
ACATACG2150.040.8139517
CATACGA2300.040.108718
TTGGGAC25000.038.885
CGTTTGG7000.038.5714262
ACACGCG2600.038.0769236
CGTTTTT18150.037.8099171
TTTCGCG4250.037.5882341
TTTGGGC24200.037.561984
GCGAATT301.1398977E-437.5000049
CGATTAC301.1398977E-437.50000412
CCCGTCG301.1398977E-437.5000041
TTACGCG601.5643309E-1037.5000041
Produced by FastQC (version 0.11.5)