##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933136.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1111799 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.258244520817165 33.0 31.0 34.0 30.0 34.0 2 32.44486098656322 34.0 31.0 34.0 31.0 34.0 3 32.5079038567223 34.0 31.0 34.0 30.0 34.0 4 35.984140118852416 37.0 35.0 37.0 35.0 37.0 5 35.2488453398501 37.0 35.0 37.0 35.0 37.0 6 34.935536009656424 37.0 35.0 37.0 32.0 37.0 7 35.70690295637971 37.0 35.0 37.0 35.0 37.0 8 35.973505102990735 37.0 35.0 37.0 35.0 37.0 9 37.832118035724086 39.0 38.0 39.0 35.0 39.0 10 37.3245694590479 39.0 37.0 39.0 34.0 39.0 11 37.224690793929476 39.0 37.0 39.0 34.0 39.0 12 37.04687448000943 39.0 37.0 39.0 33.0 39.0 13 36.983860392031296 39.0 37.0 39.0 33.0 39.0 14 38.12570347697741 40.0 37.0 41.0 33.0 41.0 15 38.23525025656616 40.0 37.0 41.0 33.0 41.0 16 38.24339921154813 40.0 37.0 41.0 34.0 41.0 17 38.20312934262398 40.0 37.0 41.0 34.0 41.0 18 38.160576686973094 40.0 37.0 41.0 34.0 41.0 19 38.16238186938467 40.0 37.0 41.0 34.0 41.0 20 38.063515077815325 40.0 37.0 41.0 34.0 41.0 21 37.95580586059171 40.0 36.0 41.0 33.0 41.0 22 37.981081112683135 40.0 36.0 41.0 33.0 41.0 23 37.89819472764412 40.0 36.0 41.0 33.0 41.0 24 37.843750534044375 40.0 36.0 41.0 33.0 41.0 25 37.738440131714455 40.0 36.0 41.0 33.0 41.0 26 37.69334654915142 40.0 36.0 41.0 33.0 41.0 27 37.66194159196042 40.0 36.0 41.0 33.0 41.0 28 37.57789312636547 40.0 36.0 41.0 33.0 41.0 29 37.530554533688196 39.0 36.0 41.0 33.0 41.0 30 37.45395525630082 39.0 36.0 41.0 33.0 41.0 31 37.38216350257556 39.0 35.0 41.0 33.0 41.0 32 37.34080530743417 39.0 35.0 41.0 33.0 41.0 33 37.23696549466226 39.0 35.0 41.0 32.0 41.0 34 37.10609471676085 39.0 35.0 41.0 32.0 41.0 35 36.986527240985104 39.0 35.0 41.0 31.0 41.0 36 36.8311637265369 39.0 35.0 41.0 31.0 41.0 37 36.81096403216768 39.0 35.0 41.0 31.0 41.0 38 36.72208825516123 39.0 35.0 41.0 31.0 41.0 39 36.68784555481701 39.0 35.0 41.0 31.0 41.0 40 36.55448421882013 39.0 35.0 41.0 31.0 41.0 41 36.42750083423353 39.0 35.0 41.0 30.0 41.0 42 36.364337438691706 39.0 35.0 40.0 30.0 41.0 43 36.23521607772628 38.0 35.0 40.0 30.0 41.0 44 36.18684402486421 38.0 35.0 40.0 30.0 41.0 45 36.18565945822941 38.0 35.0 40.0 30.0 41.0 46 35.98490734386341 38.0 35.0 40.0 30.0 41.0 47 35.89399882532724 38.0 35.0 40.0 29.0 41.0 48 35.81243821949831 38.0 35.0 40.0 29.0 41.0 49 35.84522022415922 38.0 35.0 40.0 29.0 41.0 50 35.65358396616654 38.0 34.0 40.0 29.0 41.0 51 34.53918109298533 36.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 9.0 9 15.0 10 20.0 11 18.0 12 17.0 13 15.0 14 23.0 15 34.0 16 55.0 17 109.0 18 245.0 19 445.0 20 770.0 21 1222.0 22 1806.0 23 2630.0 24 3864.0 25 5875.0 26 8320.0 27 10222.0 28 11927.0 29 14389.0 30 17835.0 31 23134.0 32 30155.0 33 42654.0 34 72742.0 35 93920.0 36 94486.0 37 140766.0 38 230482.0 39 303470.0 40 124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.567388529761224 4.566472896629697 54.711238272385565 18.154900301223513 2 19.888307149044028 4.263180664850391 53.90488748415856 21.943624701947027 3 20.552455974506184 4.400255801633208 52.44823929505243 22.599048928808173 4 18.295303377678877 4.822904140046897 52.23003438571181 24.651758096562418 5 17.90197688610981 7.589771172667002 50.148183259743895 24.360068681479298 6 22.080160172836997 6.994069971280779 53.13217586991893 17.79359398596329 7 84.69723394246623 1.9707698963571652 9.039313760850657 4.292682400325958 8 87.23348375021025 2.527255376196597 6.517724876528941 3.7215359970642172 9 81.52714654357487 5.340083954024063 8.514848457320074 4.61792104508099 10 39.78021207070703 30.25573867218805 15.393699760478288 14.570349496626639 11 30.385438375101977 24.660662583794373 25.576745436900016 19.377153604203638 12 27.18629896231243 21.37823473487564 31.11839460190196 20.317071700909967 13 24.74404096423904 22.91367414433724 32.18162635512354 20.160658536300176 14 21.1044442385719 26.043736322842527 30.97034625863128 21.88147317995429 15 19.219661107808157 23.90935771663763 35.293070060325654 21.577911115228563 16 21.76130757448064 22.031680186796354 33.58898505934976 22.61802717937325 17 21.565408855377637 21.540854057253156 30.502995595426874 26.390741491942336 18 21.938407931649515 22.320581328099774 31.060830239998417 24.680180500252295 19 24.137096723418534 23.39991311379125 29.370686607921037 23.092303554869183 20 27.402525096712626 23.737294241135313 28.336596812913122 20.523583849238936 21 26.319955315664075 24.73693536331657 27.589519328583673 21.353589992435683 22 23.852063187680507 23.52169771694344 27.648612743850283 24.97762635152577 23 22.977624552639462 24.78532540504174 27.35773282760643 24.879317214712373 24 22.620815453152954 23.64600076092891 29.84334398573843 23.889839800179708 25 22.897394223236393 24.029163544849382 28.012257611312837 25.061184620601384 26 21.212287472825576 25.683599283683474 28.340374474163042 24.763738769327908 27 21.698796275226005 25.329758346607615 28.861421893705607 24.110023484460772 28 20.59203147331487 24.122885521573593 31.146097451068044 24.13898555404349 29 21.414212461065354 22.888669624635387 30.420336769506 25.276781144793258 30 22.896135002819754 23.729828862950946 29.71652250091968 23.65751363330962 31 23.983471832588442 25.59194602621517 26.563974243545818 23.860607897650567 32 23.842798923186656 25.656256211779287 26.856473157468212 23.644471707565845 33 23.929685131934818 24.765087934060023 26.978977315144192 24.326249618860963 34 22.580610344135945 24.123964853359286 28.830750882128875 24.464673920375894 35 22.340908743396962 23.093202998023923 28.879590645431414 25.6862976131477 36 23.098689601267854 24.765087934060023 28.435625504250318 23.7005969604218 37 23.43094390262988 25.478436300086614 27.7818202750677 23.308799522215796 38 22.599408706070072 25.31248903803655 27.09959264219522 24.98850961369816 39 22.372029476551067 24.229829312672525 29.036903253195945 24.36123795758046 40 22.748896158388344 24.167497902048844 30.58097731694308 22.502628622619735 41 20.886868939439594 24.97906546057336 29.420155981431893 24.713909618555153 42 22.678919480949343 23.89847445446524 28.731722190791682 24.690883873793734 43 22.27983655318992 23.428065684534705 29.241796403846376 25.050301358428996 44 22.346665179587315 23.07494430198264 28.893262181383506 25.685128337046535 45 22.112989847985112 23.648159424500292 27.634851263582718 26.60399946393188 46 22.38552112387221 24.476996291595874 28.64438626046615 24.49309632406577 47 20.995431728217063 24.50361980897626 29.995709656151874 24.5052388066548 48 21.339019013328848 23.240082065193437 30.182703888022928 25.238195033454787 49 22.135655815484633 22.92959428817619 30.67577862545298 24.258971270886196 50 21.84459601060983 22.64635963874765 29.153111308788727 26.355933041853792 51 20.427163543050497 23.35521078900053 27.983925151938433 28.23370051601054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 185.0 1 859.0 2 1533.0 3 3528.0 4 5523.0 5 3945.5 6 2368.0 7 2302.5 8 2237.0 9 2253.0 10 2269.0 11 2278.5 12 2288.0 13 2247.5 14 2207.0 15 2159.5 16 2112.0 17 2166.0 18 2220.0 19 2556.0 20 2892.0 21 2952.0 22 3012.0 23 3685.0 24 4358.0 25 4811.0 26 7504.5 27 9745.0 28 10924.5 29 12104.0 30 14406.0 31 16708.0 32 19391.5 33 22075.0 34 24918.5 35 27762.0 36 31210.5 37 34659.0 38 36240.0 39 37821.0 40 43178.5 41 48536.0 42 55278.5 43 62021.0 44 67707.0 45 73393.0 46 81836.5 47 90280.0 48 98436.5 49 106593.0 50 105471.0 51 104349.0 52 93606.0 53 82863.0 54 75480.0 55 68097.0 56 62882.5 57 57668.0 58 55342.0 59 53016.0 60 49364.5 61 45713.0 62 40104.0 63 34495.0 64 30206.5 65 25918.0 66 22152.0 67 18386.0 68 16245.0 69 14104.0 70 12192.0 71 10280.0 72 8456.5 73 6633.0 74 5564.0 75 3446.0 76 2397.0 77 1987.0 78 1577.0 79 1227.5 80 878.0 81 646.5 82 415.0 83 298.5 84 182.0 85 130.0 86 78.0 87 58.0 88 38.0 89 37.5 90 37.0 91 25.5 92 14.0 93 7.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1111799.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.086939023240618 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49002138131652 23.46753691544117 2 10.805477390016163 6.718184334808752 3 3.793025023385674 3.5374061284684877 4 1.7222014817743472 2.141518897986151 5 1.0089958251844844 1.5683295846107206 6 0.6465726211299344 1.2059978188297877 7 0.4255831871546793 0.9261055011875738 8 0.3275173991108475 0.814521073216742 9 0.27881805825463496 0.7800839977986167 >10 4.679148985400148 39.16341075503533 >50 0.7424846081267337 14.165605015763354 >100 0.07621664774942802 3.908365608568385 >500 0.002531180183447349 0.49091722907822916 >1k 0.0014062112130263048 1.1120171392067668 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC 3000 0.2698329464228696 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2807 0.25247369353633164 No Hit CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT 2684 0.24141054273299398 Illumina Single End Adapter 1 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC 2206 0.19841715993628345 Illumina Single End Adapter 1 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC 2022 0.18186740588901412 Illumina Single End Adapter 1 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.994431547428987E-5 0.0 0.0 0.2582301297266862 0.0 2 8.994431547428987E-5 0.0 0.0 0.9767952660507879 0.0 3 8.994431547428987E-5 0.0 0.0 1.4012424907739618 0.0 4 8.994431547428987E-5 0.0 0.0 1.9093379288882253 0.0 5 8.994431547428987E-5 0.0 0.0 2.726661923603097 0.0 6 8.994431547428987E-5 0.0 0.0 3.309051366299124 0.0 7 8.994431547428987E-5 0.0 0.0 3.850426201138875 0.0 8 8.994431547428987E-5 0.0 0.0 4.749689467250825 0.0 9 8.994431547428987E-5 0.0 0.0 5.277032988876586 0.0 10 8.994431547428987E-5 0.0 0.0 6.163793995137611 0.0 11 8.994431547428987E-5 0.0 0.0 7.680884764242458 0.0 12 8.994431547428987E-5 0.0 0.0 9.104613333884991 0.0 13 8.994431547428987E-5 0.0 0.0 9.534906939113995 0.0 14 8.994431547428987E-5 0.0 0.0 9.6734211849444 0.0 15 8.994431547428987E-5 0.0 0.0 9.89270542607072 8.994431547428987E-5 16 8.994431547428987E-5 0.0 0.0 10.41834000570247 8.994431547428987E-5 17 8.994431547428987E-5 0.0 0.0 11.225860070030643 8.994431547428987E-5 18 1.7988863094857974E-4 0.0 0.0 12.130519995071051 8.994431547428987E-5 19 1.7988863094857974E-4 0.0 0.0 12.717226764909844 8.994431547428987E-5 20 1.7988863094857974E-4 0.0 0.0 13.329387776027861 8.994431547428987E-5 21 1.7988863094857974E-4 0.0 0.0 14.122696638511098 8.994431547428987E-5 22 1.7988863094857974E-4 0.0 0.0 14.966014540398039 8.994431547428987E-5 23 1.7988863094857974E-4 0.0 0.0 15.779920651124888 8.994431547428987E-5 24 1.7988863094857974E-4 0.0 0.0 16.428778942956416 8.994431547428987E-5 25 1.7988863094857974E-4 0.0 0.0 16.983735369432782 8.994431547428987E-5 26 1.7988863094857974E-4 0.0 0.0 17.486344204303116 8.994431547428987E-5 27 1.7988863094857974E-4 0.0 0.0 17.969974788608372 8.994431547428987E-5 28 1.7988863094857974E-4 0.0 0.0 18.497318310234135 8.994431547428987E-5 29 1.7988863094857974E-4 0.0 0.0 19.052364681025978 8.994431547428987E-5 30 1.7988863094857974E-4 0.0 0.0 19.695826313929047 8.994431547428987E-5 31 1.7988863094857974E-4 0.0 0.0 20.26814199329195 8.994431547428987E-5 32 1.7988863094857974E-4 0.0 0.0 20.838658786345373 8.994431547428987E-5 33 1.7988863094857974E-4 0.0 0.0 21.367081639756826 8.994431547428987E-5 34 1.7988863094857974E-4 0.0 0.0 21.913043634685767 8.994431547428987E-5 35 1.7988863094857974E-4 0.0 0.0 22.52664375485137 8.994431547428987E-5 36 1.7988863094857974E-4 0.0 0.0 23.099049378529752 8.994431547428987E-5 37 1.7988863094857974E-4 0.0 0.0 23.648609146077664 8.994431547428987E-5 38 1.7988863094857974E-4 0.0 0.0 24.19115325701858 8.994431547428987E-5 39 1.7988863094857974E-4 0.0 0.0 24.77174381340512 8.994431547428987E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATA 20 7.033186E-4 45.000004 16 ACGATTA 20 7.033186E-4 45.000004 11 GCGATAT 20 7.033186E-4 45.000004 9 CCGATCA 35 1.2118835E-7 45.0 36 CGTTGAA 25 3.8907237E-5 45.0 18 CATCCGC 25 3.8907237E-5 45.0 41 TACGAAA 205 0.0 42.80488 20 ATACGAA 230 0.0 41.08696 19 ACATACG 215 0.0 40.81395 17 CATACGA 230 0.0 40.1087 18 TTGGGAC 2500 0.0 38.88 5 CGTTTGG 700 0.0 38.571426 2 ACACGCG 260 0.0 38.07692 36 CGTTTTT 1815 0.0 37.809917 1 TTTCGCG 425 0.0 37.588234 1 TTTGGGC 2420 0.0 37.56198 4 GCGAATT 30 1.1398977E-4 37.500004 9 CGATTAC 30 1.1398977E-4 37.500004 12 CCCGTCG 30 1.1398977E-4 37.500004 1 TTACGCG 60 1.5643309E-10 37.500004 1 >>END_MODULE