##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933134.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1604937
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28064092235396	33.0	31.0	34.0	30.0	34.0
2	32.47028201106959	34.0	31.0	34.0	31.0	34.0
3	32.54119445186945	34.0	31.0	34.0	31.0	34.0
4	36.00020686170236	37.0	35.0	37.0	35.0	37.0
5	35.19571484737407	37.0	35.0	37.0	35.0	37.0
6	34.86452490035435	37.0	35.0	37.0	32.0	37.0
7	35.63913848331742	37.0	35.0	37.0	33.0	37.0
8	35.919930813483646	37.0	35.0	37.0	35.0	37.0
9	37.799912395315204	39.0	38.0	39.0	35.0	39.0
10	37.33135381638033	39.0	37.0	39.0	34.0	39.0
11	37.25534522538891	39.0	37.0	39.0	34.0	39.0
12	37.0691615932588	39.0	37.0	39.0	33.0	39.0
13	37.023435810876066	39.0	37.0	39.0	33.0	39.0
14	38.21232733745935	40.0	37.0	41.0	33.0	41.0
15	38.29642658870722	40.0	37.0	41.0	33.0	41.0
16	38.28391893264346	40.0	37.0	41.0	34.0	41.0
17	38.21008488183649	40.0	37.0	41.0	34.0	41.0
18	38.17312019101061	40.0	37.0	41.0	34.0	41.0
19	38.16488248448381	40.0	37.0	41.0	34.0	41.0
20	38.08311291969716	40.0	37.0	41.0	34.0	41.0
21	37.953064201273946	40.0	36.0	41.0	33.0	41.0
22	37.97617725804813	40.0	36.0	41.0	33.0	41.0
23	37.89444757021615	40.0	36.0	41.0	33.0	41.0
24	37.81332475978808	40.0	36.0	41.0	33.0	41.0
25	37.69587528980888	40.0	36.0	41.0	33.0	41.0
26	37.64404771028396	40.0	36.0	41.0	33.0	41.0
27	37.59279710044693	40.0	36.0	41.0	33.0	41.0
28	37.487888932712	40.0	35.0	41.0	33.0	41.0
29	37.39673893741624	39.0	35.0	41.0	33.0	41.0
30	37.30583131923559	39.0	35.0	41.0	32.0	41.0
31	37.23573448677425	39.0	35.0	41.0	32.0	41.0
32	37.196012678379276	39.0	35.0	41.0	32.0	41.0
33	37.05695176820025	39.0	35.0	41.0	32.0	41.0
34	36.89667257967135	39.0	35.0	41.0	31.0	41.0
35	36.737166630216635	39.0	35.0	41.0	31.0	41.0
36	36.60128092255334	39.0	35.0	41.0	31.0	41.0
37	36.556427448554054	39.0	35.0	41.0	31.0	41.0
38	36.44893724800413	39.0	35.0	41.0	30.0	41.0
39	36.39272320346531	39.0	35.0	41.0	30.0	41.0
40	36.26437112484789	39.0	35.0	40.0	30.0	41.0
41	36.11691549263304	38.0	35.0	40.0	30.0	41.0
42	36.03438951186246	38.0	35.0	40.0	30.0	41.0
43	35.89530928628351	38.0	35.0	40.0	29.0	41.0
44	35.81203561261283	38.0	35.0	40.0	29.0	41.0
45	35.8076217321926	38.0	35.0	40.0	29.0	41.0
46	35.57480449388356	38.0	34.0	40.0	28.0	41.0
47	35.484486930016566	37.0	34.0	40.0	28.0	41.0
48	35.38825199992274	37.0	34.0	40.0	28.0	41.0
49	35.397519030342	37.0	34.0	40.0	28.0	41.0
50	35.212288706659514	37.0	34.0	40.0	28.0	41.0
51	34.15959442644789	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	23.0
10	38.0
11	39.0
12	31.0
13	37.0
14	49.0
15	69.0
16	143.0
17	257.0
18	437.0
19	767.0
20	1360.0
21	2130.0
22	3112.0
23	4608.0
24	6706.0
25	9610.0
26	13362.0
27	16473.0
28	18903.0
29	22182.0
30	27467.0
31	34644.0
32	44354.0
33	62825.0
34	106655.0
35	132804.0
36	145099.0
37	212251.0
38	326004.0
39	412341.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.06902140084003	4.561175921547076	52.363363795588235	18.006438882024653
2	20.488717002598854	4.471764312244033	53.07466897454541	21.964849710611695
3	21.789266494572683	4.832526136539939	51.17054438896979	22.207662979917593
4	18.53356237659173	5.079950178729757	50.732458657255705	25.65402878742281
5	17.729543278022753	7.893269330821086	49.46648996191128	24.910697429244884
6	22.446862400206363	7.860495458700248	52.272020646293285	17.420621494800105
7	85.43942846354716	2.187250963744994	8.450674387842016	3.922646184865823
8	87.67284946387304	2.8229768520508904	6.348785030191216	3.155388653884857
9	82.64866471394204	4.8877307956636304	7.801988489267803	4.661616001126524
10	41.09525794470437	27.978917552526983	14.908871812413821	16.016952690354824
11	31.797821347504605	24.736298060297692	22.751048795061738	20.714831797135965
12	27.355092442880935	21.95718585838572	29.23759624209548	21.45012545663786
13	25.081919103366673	22.52262861408267	30.471974912410893	21.923477370139764
14	22.15762986335289	23.90168586056649	31.00532918114543	22.935355094935193
15	21.38189847950418	22.137878309242044	33.325669481107354	23.154553730146418
16	23.628653336548414	21.298343797918548	31.59488503287045	23.47811783266259
17	22.875788893894278	20.854837292678777	30.227853180529827	26.041520632897118
18	24.215093801189706	21.26108376839714	29.736432021942292	24.78739040847086
19	25.805498907433748	23.723859565827194	27.09346223558931	23.377179291149748
20	29.07515995954981	22.447111631173062	26.875198216503204	21.602530192773923
21	27.62606881142375	23.720744178743463	25.707426522037935	22.945760487794846
22	26.444464798306726	22.534654008225868	25.987125974415193	25.03375521905221
23	26.485774831036977	23.811962712555072	25.744250397367622	23.958012059040325
24	24.872191244889986	23.215802240212543	27.773052774034117	24.138953740863347
25	24.83374736827676	23.74130573349608	26.21878615796134	25.206160740265815
26	23.854581207860495	24.597601027330047	26.490136372954204	25.057681391855258
27	23.868911988445653	24.358401607041273	26.78242198914973	24.990264415363345
28	22.90837584279009	25.069146016323383	27.88639055614021	24.136087584746317
29	23.997328244036993	23.70734801428343	26.804042775510815	25.491280966168766
30	24.943782840074096	23.57139252194946	27.309109329525082	24.17571530845136
31	25.65471417258123	25.017929052666865	24.96851901351891	24.358837761232994
32	25.867495110399975	24.524638661829094	25.22796844985192	24.37989777791901
33	25.931049006908058	24.412360111331473	24.68483186567448	24.97175901608599
34	24.41697088421539	24.398465484938036	26.549702573995116	24.634861056851452
35	23.971283608017014	24.576914857094078	26.026753698120235	25.425047836768673
36	25.0862806452839	27.225803878906152	24.146430669864298	23.541484805945654
37	25.22884075823537	26.380973209540315	24.923968978221573	23.46621705400274
38	24.976805943161633	25.723564227131657	25.532341767932326	23.767288061774387
39	25.014128280424714	24.165621454300076	26.071864503092645	24.748385762182565
40	25.13593991539855	24.37067623215117	27.627314966257245	22.86606888619304
41	22.318072298164974	25.374391642787224	27.02959680037285	25.27793925867495
42	23.984181310543654	25.527606379565054	25.330464684906634	25.157747624984655
43	24.397904715262968	23.49762015580674	26.493812529713008	25.61066259921729
44	24.24431613203509	24.247244595893797	26.19679152515021	25.311647746920908
45	24.171229151050788	23.435437029615493	25.690914970494166	26.70241884883955
46	23.92218510757743	24.706764190743936	26.222088468270094	25.14896223340854
47	22.71653030617401	25.736275006433274	27.441201741875226	24.10599294551749
48	23.727847261294368	24.196152247720626	27.279886998679697	24.796113492305306
49	23.973775917683994	24.43603705316782	27.11931994838427	24.470867080763917
50	23.266769972902363	24.215903801831473	27.180319227483697	25.337006997782467
51	22.452843943407125	24.54439021593994	26.04563294384764	26.957132896805298
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	259.0
1	1270.0
2	2281.0
3	5826.0
4	9371.0
5	6557.0
6	3743.0
7	3604.5
8	3466.0
9	3510.0
10	3554.0
11	3628.0
12	3702.0
13	3656.5
14	3611.0
15	3449.5
16	3288.0
17	3253.5
18	3219.0
19	3580.0
20	3941.0
21	4106.5
22	4272.0
23	4872.0
24	5472.0
25	6235.0
26	8894.5
27	10791.0
28	13229.5
29	15668.0
30	17463.0
31	19258.0
32	22732.0
33	26206.0
34	31172.0
35	36138.0
36	38592.0
37	41046.0
38	45008.0
39	48970.0
40	54585.5
41	60201.0
42	65878.5
43	71556.0
44	77728.5
45	83901.0
46	89408.0
47	94915.0
48	103236.5
49	111558.0
50	120716.0
51	129874.0
52	130163.5
53	130453.0
54	122511.5
55	114570.0
56	109069.0
57	103568.0
58	100891.0
59	98214.0
60	93227.0
61	88240.0
62	80008.5
63	71777.0
64	62872.5
65	53968.0
66	47709.0
67	41450.0
68	36122.0
69	30794.0
70	26908.5
71	23023.0
72	20108.5
73	17194.0
74	14036.5
75	8801.0
76	6723.0
77	5304.5
78	3886.0
79	2755.0
80	1624.0
81	1247.5
82	871.0
83	566.5
84	262.0
85	173.5
86	85.0
87	68.0
88	51.0
89	32.0
90	13.0
91	17.0
92	21.0
93	14.5
94	8.0
95	4.5
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1604937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.438604236183334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77052553546385	22.116851969430463
2	8.677158391252515	4.935325467670151
3	2.7950326102112975	2.3846047868707676
4	1.3366909760099281	1.5205450261129592
5	0.764744157957067	1.087412822503715
6	0.5186217934918914	0.8849327960025302
7	0.4359554041900542	0.8678574243070405
8	0.3369341193358463	0.766554885876728
9	0.30032879707443205	0.768683862065591
>10	6.253258469604145	47.544054444400565
>50	0.7444641365770469	12.720359704317538
>100	0.06148862451372327	2.9542273376363988
>500	0.0032706711260947993	0.5948511969788933
>1k	0.0015263131921775732	0.8537382758266866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4808	0.2995756219714543	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	1852	0.11539393758135054	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	1789	0.11146854985585104	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	1663	0.10361777440485204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2461548334919065E-4	0.0	0.0	0.12492702205756363	0.0
2	1.2461548334919065E-4	0.0	0.0	0.46219882774214816	0.0
3	1.2461548334919065E-4	0.0	0.0	0.6795282307031366	0.0
4	1.2461548334919065E-4	0.0	0.0	0.9628415320975215	0.0
5	1.2461548334919065E-4	0.0	0.0	1.4528919203682138	0.0
6	1.2461548334919065E-4	0.0	0.0	1.8704784050713517	0.0
7	1.2461548334919065E-4	0.0	0.0	2.233047153875822	0.0
8	1.2461548334919065E-4	0.0	0.0	2.817369155300177	0.0
9	1.2461548334919065E-4	0.0	0.0	3.1714017434952275	0.0
10	1.8692322502378598E-4	0.0	0.0	3.76531913713747	0.0
11	1.8692322502378598E-4	0.0	0.0	4.672021393986181	0.0
12	1.8692322502378598E-4	0.0	0.0	5.515169754326806	0.0
13	1.8692322502378598E-4	0.0	0.0	5.7749930371098674	0.0
14	1.8692322502378598E-4	0.0	0.0	5.869950035421951	0.0
15	1.8692322502378598E-4	0.0	0.0	6.015874766423853	0.0
16	1.8692322502378598E-4	0.0	0.0	6.347788106324423	0.0
17	1.8692322502378598E-4	0.0	0.0	6.7973384625066275	0.0
18	1.8692322502378598E-4	0.0	0.0	7.297545012670279	0.0
19	1.8692322502378598E-4	0.0	0.0	7.624536040978556	0.0
20	1.8692322502378598E-4	0.0	0.0	7.986606327849629	0.0
21	2.492309666983813E-4	0.0	0.0	8.486750570271605	0.0
22	2.492309666983813E-4	0.0	0.0	9.033314080241157	0.0
23	2.492309666983813E-4	0.0	0.0	9.554518339349146	0.0
24	2.492309666983813E-4	0.0	0.0	9.978896367894814	0.0
25	2.492309666983813E-4	0.0	0.0	10.348443583766839	0.0
26	2.492309666983813E-4	0.0	0.0	10.675870766266838	0.0
27	2.492309666983813E-4	0.0	0.0	11.002737179091765	0.0
28	2.492309666983813E-4	0.0	0.0	11.339572830584627	0.0
29	2.492309666983813E-4	0.0	0.0	11.699275423272066	0.0
30	2.492309666983813E-4	0.0	0.0	12.12072498795903	0.0
31	3.1153870837297663E-4	0.0	0.0	12.509525296008503	0.0
32	3.1153870837297663E-4	0.0	0.0	12.899820989858169	0.0
33	3.1153870837297663E-4	0.0	0.0	13.28313821664028	0.0
34	3.1153870837297663E-4	0.0	0.0	13.651439277678813	0.0
35	3.1153870837297663E-4	0.0	0.0	14.064103450789657	0.0
36	3.7384645004757196E-4	0.0	0.0	14.445551445321529	0.0
37	3.7384645004757196E-4	0.0	0.0	14.827124055336752	0.0
38	3.7384645004757196E-4	0.0	0.0	15.218229749828186	0.0
39	3.7384645004757196E-4	0.0	0.0	15.617933912670715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1660417E-6	45.000004	9
ATCGGAA	20	7.034123E-4	45.0	37
ACGACCG	40	3.4593722E-7	39.375	38
TTGGGAC	3000	0.0	39.075	5
TTTGGGA	7685	0.0	38.70527	4
GTCGACA	35	6.2498366E-6	38.57143	13
TACGGGA	335	0.0	38.283585	4
TCGTTTG	710	0.0	38.028168	1
TCAAGCG	1055	0.0	37.962086	17
CGTTTTT	2825	0.0	37.911507	1
CGTTTGG	1190	0.0	37.815125	2
TTTGGGC	4225	0.0	37.54438	4
AATGCGT	30	1.1401235E-4	37.500004	36
AACACGT	985	0.0	37.461926	41
ACATACG	410	0.0	37.317074	17
CGACCAA	1065	0.0	37.1831	29
ACACGAC	1060	0.0	37.146225	26
TGGGCGA	2770	0.0	37.12094	6
GACACGA	1075	0.0	36.83721	25
CACGACC	1070	0.0	36.799065	27
>>END_MODULE