##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933133.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1372997
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21683222905804	33.0	31.0	34.0	30.0	34.0
2	32.41185596181201	34.0	31.0	34.0	30.0	34.0
3	32.47930112010442	34.0	31.0	34.0	30.0	34.0
4	35.94931452872803	37.0	35.0	37.0	35.0	37.0
5	35.16120574189164	37.0	35.0	37.0	35.0	37.0
6	34.82656844843798	37.0	35.0	37.0	32.0	37.0
7	35.60474786179431	37.0	35.0	37.0	33.0	37.0
8	35.8947128070928	37.0	35.0	37.0	35.0	37.0
9	37.76230392346087	39.0	38.0	39.0	35.0	39.0
10	37.29252503829215	39.0	37.0	39.0	34.0	39.0
11	37.18056121025756	39.0	37.0	39.0	34.0	39.0
12	36.914037685442864	39.0	35.0	39.0	33.0	39.0
13	36.78510368194541	39.0	35.0	39.0	33.0	39.0
14	37.68476988660572	40.0	37.0	41.0	33.0	41.0
15	37.87026992775658	40.0	37.0	41.0	33.0	41.0
16	37.95527229848281	40.0	37.0	41.0	33.0	41.0
17	37.9256699031389	40.0	36.0	41.0	33.0	41.0
18	37.908583922616	40.0	36.0	41.0	33.0	41.0
19	37.909722308206064	40.0	36.0	41.0	33.0	41.0
20	37.786696547771044	40.0	36.0	41.0	33.0	41.0
21	37.650190058681844	40.0	36.0	41.0	33.0	41.0
22	37.733014711612626	40.0	36.0	41.0	33.0	41.0
23	37.69069488134351	39.0	35.0	41.0	33.0	41.0
24	37.607163744713205	39.0	35.0	41.0	33.0	41.0
25	37.480542929081416	39.0	35.0	41.0	33.0	41.0
26	37.441311962080036	39.0	35.0	41.0	33.0	41.0
27	37.38575539494988	39.0	35.0	41.0	33.0	41.0
28	37.27810767248581	39.0	35.0	41.0	32.0	41.0
29	37.18759618557069	39.0	35.0	41.0	32.0	41.0
30	37.083801348437035	39.0	35.0	41.0	32.0	41.0
31	36.95585350878407	39.0	35.0	41.0	31.0	41.0
32	36.91884614460192	39.0	35.0	41.0	31.0	41.0
33	36.80708916334122	39.0	35.0	41.0	31.0	41.0
34	36.6268870216031	39.0	35.0	41.0	31.0	41.0
35	36.49547231348648	39.0	35.0	41.0	30.0	41.0
36	36.358879152685695	39.0	35.0	41.0	30.0	41.0
37	36.28374060540555	39.0	35.0	40.0	30.0	41.0
38	36.13744385457507	38.0	35.0	40.0	30.0	41.0
39	36.12256326852863	38.0	35.0	40.0	30.0	41.0
40	35.99902985949714	38.0	35.0	40.0	30.0	41.0
41	35.82409429882221	38.0	35.0	40.0	29.0	41.0
42	35.75969503210859	38.0	35.0	40.0	29.0	41.0
43	35.62336479977742	38.0	35.0	40.0	29.0	41.0
44	35.543862805235555	38.0	34.0	40.0	29.0	41.0
45	35.50800766498397	37.0	34.0	40.0	29.0	41.0
46	35.32820537845312	37.0	34.0	40.0	28.0	41.0
47	35.204924701219305	37.0	34.0	40.0	28.0	41.0
48	35.11841686471274	37.0	34.0	40.0	28.0	41.0
49	35.16660269468906	37.0	34.0	40.0	28.0	41.0
50	34.971083695011714	36.0	34.0	40.0	27.0	41.0
51	33.906452818178046	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	41.0
10	41.0
11	33.0
12	28.0
13	26.0
14	44.0
15	60.0
16	123.0
17	224.0
18	412.0
19	765.0
20	1244.0
21	2058.0
22	2998.0
23	4389.0
24	6234.0
25	9161.0
26	12546.0
27	15098.0
28	17815.0
29	20983.0
30	26040.0
31	33215.0
32	43154.0
33	62118.0
34	102549.0
35	113007.0
36	124577.0
37	180929.0
38	277884.0
39	315081.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.323289125904864	4.803797823301871	52.8219653793854	17.050947671407876
2	21.225173835048437	4.348225087163337	51.153134347707976	23.273466730080255
3	21.711992087382566	4.815232662562263	51.043447290853514	22.429327959201657
4	18.984600840351433	5.008168262567216	49.999453749716864	26.007777147364486
5	18.024657009447218	8.263091616369154	48.865073995063355	24.847177379120275
6	22.729547114815254	7.834248727418924	51.363258623289056	18.07294553447677
7	83.52960712951302	2.3499687180671187	9.407449542861347	4.712974609558506
8	86.15932882591878	2.6261528612225664	7.294116447450359	3.9204018654083
9	80.8150345557929	4.8171991635815665	9.394558036179248	4.9732082444462735
10	42.71779180872209	25.44186185403173	16.12691069244871	15.713435644797476
11	33.082810814590275	23.168805175830684	23.35394760512951	20.394436404449536
12	27.72191053585696	21.060861749880008	30.30669404230308	20.910533671959953
13	26.254245275117132	22.103835623821467	31.81915182626036	19.822767274801038
14	21.974483556773976	23.365600944503157	33.97902544579486	20.68089005292801
15	20.766323597211063	20.50324946085097	35.97932114928146	22.751105792656503
16	25.910690263707785	20.154377613352395	31.2017433395703	22.73318878336952
17	25.200710562368307	20.89312649627057	28.94267066861763	24.963492272743494
18	26.999039327835384	19.68780703817998	29.905673501107433	23.4074801328772
19	26.76881304183476	22.56698303055287	26.90464727890884	23.75955664870353
20	27.95978432582154	24.674198122792692	26.475076056247755	20.89094149513801
21	27.263570131617186	23.639745753268215	28.18083360706542	20.91585050804918
22	25.469465701673055	20.98059937494401	28.94915283864422	24.600782084738714
23	24.224889056567495	23.354603105469277	28.329413684079423	24.0910941538838
24	24.222558388692764	24.090365820172952	28.577993979593547	23.109081811540737
25	25.829626721689852	24.046592964150687	25.563930583970684	24.559849730188777
26	24.111851664643112	23.397064960812006	25.84004189375505	26.651041480789832
27	23.399177128573477	21.663339395497587	29.146968274511885	25.790515201417048
28	21.716434923018767	23.563052213515398	28.154395093361455	26.566117770104373
29	22.99458775219465	23.50245484877243	27.189789926707782	26.313167472325137
30	26.198090746010372	21.288538867892644	29.039247718676737	23.47412266742025
31	26.033487327357598	23.186285184891155	27.39292219866467	23.387305289086573
32	25.710762660078647	22.74739129073115	26.68374366440713	24.85810238478307
33	25.577550424363636	22.306530895551848	25.717754663702834	26.39816401638168
34	21.809880138121205	23.2682955607332	29.82286195818345	25.09896234296215
35	22.34724474998853	25.291315275998414	27.695472022153	24.665967951860054
36	24.992407121064357	25.551913077741613	25.406319168942105	24.04936063225193
37	23.332461760659346	25.242444084000184	27.5181227635603	23.90697139178017
38	23.147246497989435	25.44259018774258	27.789427070853034	23.620736243414953
39	23.05751578481235	22.6520524079805	26.617392463348427	27.673039343858726
40	25.27966193662477	22.523137341159522	27.79925957594955	24.39794114626616
41	21.76814661648933	23.3827167867082	26.77493104500592	28.074205551796545
42	22.725031445807968	23.411631635029064	27.33276183414822	26.530575085014753
43	23.23420954306528	21.81366747341764	28.727957890658175	26.224165092858907
44	24.14870535041227	21.86792833487619	28.343397691327805	25.639968623383737
45	23.22554237190613	21.952924878932727	26.71061917833761	28.110913570823538
46	23.289417238347934	21.89589634937294	28.283747160408947	26.53093925187018
47	20.80587211771038	24.437926666991988	30.52818032377347	24.228020891524164
48	21.756784610600022	24.607919755105073	28.514847446862596	25.12044818743231
49	23.56669388206966	22.722409444448896	28.97114851671198	24.739748156769462
50	22.1601358196704	23.24957738436428	27.956215490638364	26.634071305326962
51	21.636682381680366	22.899613036299424	26.87755326486511	28.5861513171551
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	191.0
1	826.5
2	1462.0
3	4726.0
4	7990.0
5	5380.0
6	2770.0
7	2708.5
8	2647.0
9	2724.5
10	2802.0
11	2844.5
12	2887.0
13	2906.5
14	2926.0
15	2711.5
16	2497.0
17	2537.0
18	2577.0
19	2854.5
20	3132.0
21	3097.0
22	3062.0
23	3871.0
24	4680.0
25	5291.5
26	7457.0
27	9011.0
28	9664.5
29	10318.0
30	12709.0
31	15100.0
32	18362.0
33	21624.0
34	25678.0
35	29732.0
36	31896.0
37	34060.0
38	37135.0
39	40210.0
40	44275.0
41	48340.0
42	52949.0
43	57558.0
44	64038.0
45	70518.0
46	82123.5
47	93729.0
48	107323.5
49	120918.0
50	122869.0
51	124820.0
52	116418.5
53	108017.0
54	104054.0
55	100091.0
56	94664.5
57	89238.0
58	83669.5
59	78101.0
60	73392.5
61	68684.0
62	62504.0
63	56324.0
64	51179.5
65	46035.0
66	39971.5
67	33908.0
68	28607.0
69	23306.0
70	19909.5
71	16513.0
72	14460.0
73	12407.0
74	10280.0
75	6797.0
76	5441.0
77	4205.0
78	2969.0
79	2101.0
80	1233.0
81	940.5
82	648.0
83	458.0
84	268.0
85	180.5
86	93.0
87	66.0
88	39.0
89	44.0
90	49.0
91	32.0
92	15.0
93	8.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1372997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.601997786470108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23470367694182	23.635362309604485
2	9.073908389184272	5.553594488808993
3	2.9961923048388286	2.750684108415498
4	1.4765908468004816	1.8074651930124142
5	0.8778187139769608	1.3431503171022496
6	0.6357300312102578	1.1672765404733327
7	0.4700426090462245	1.0068970017105439
8	0.3739332835532004	0.9154484412466031
9	0.30749399278398504	0.8468937437875529
>10	6.066846879972725	47.5882489958799
>50	0.4160646475967598	7.713853629409955
>100	0.06500174461798132	3.572211424320204
>500	0.0035455496728684964	0.7626166819128312
>1k	0.0021273298037210978	1.336297124315436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4615	0.33612600755864724	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2202	0.16037908312982477	TruSeq Adapter, Index 23 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2103	0.15316857939238032	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	2082	0.15163907859958908	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2066	0.15047374466222432	TruSeq Adapter, Index 23 (95% over 23bp)
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	1506	0.1096870568544578	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	1448	0.10546272133151056	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.641668554264867E-4	0.0	0.0	0.1613259169539336	0.0
2	3.641668554264867E-4	0.0	0.0	0.67603935041373	0.0
3	3.641668554264867E-4	0.0	0.0	1.0217793629556364	0.0
4	3.641668554264867E-4	0.0	0.0	1.4306659082284958	0.0
5	3.641668554264867E-4	0.0	0.0	2.1509879482620864	0.0
6	3.641668554264867E-4	0.0	0.0	2.769853102373858	0.0
7	3.641668554264867E-4	0.0	0.0	3.320182054294365	0.0
8	3.641668554264867E-4	0.0	0.0	4.181655167491262	0.0
9	3.641668554264867E-4	0.0	0.0	4.718728445874245	0.0
10	3.641668554264867E-4	0.0	0.0	5.519531360957089	0.0
11	3.641668554264867E-4	0.0	0.0	6.73249832301163	0.0
12	3.641668554264867E-4	0.0	0.0	7.700162491250891	0.0
13	3.641668554264867E-4	0.0	0.0	8.070738683332884	0.0
14	3.641668554264867E-4	0.0	0.0	8.223178929014411	0.0
15	3.641668554264867E-4	0.0	0.0	8.441606208899218	0.0
16	4.370002265117841E-4	0.0	0.0	8.846851085617812	0.0
17	4.370002265117841E-4	0.0	0.0	9.422307550562747	0.0
18	4.370002265117841E-4	0.0	0.0	10.042046705127541	0.0
19	4.370002265117841E-4	0.0	0.0	10.441901912385825	0.0
20	4.370002265117841E-4	0.0	0.0	10.896163647844824	0.0
21	4.370002265117841E-4	0.0	0.0	11.484438786100771	0.0
22	4.370002265117841E-4	0.0	0.0	12.130616454369529	0.0
23	4.370002265117841E-4	0.0	0.0	12.73090909885455	0.0
24	5.098335975970814E-4	0.0	0.0	13.2285066901093	0.0
25	5.826669686823788E-4	0.0	0.0	13.678179923189926	0.0
26	5.826669686823788E-4	0.0	0.0	14.069586459402315	0.0
27	5.826669686823788E-4	0.0	0.0	14.515618023928676	0.0
28	5.826669686823788E-4	0.0	0.0	14.943805412539138	0.0
29	5.826669686823788E-4	0.0	0.0	15.454950010815756	0.0
30	5.826669686823788E-4	0.0	0.0	16.092460507925363	0.0
31	5.826669686823788E-4	0.0	0.0	16.522104563957534	0.0
32	5.826669686823788E-4	0.0	0.0	16.98459647034917	0.0
33	5.826669686823788E-4	0.0	0.0	17.439586539519023	0.0
34	5.826669686823788E-4	0.0	0.0	17.947453636096803	0.0
35	6.555003397676761E-4	0.0	0.0	18.5290281042129	0.0
36	6.555003397676761E-4	0.0	0.0	18.984746507093607	0.0
37	6.555003397676761E-4	0.0	0.0	19.45131708226602	0.0
38	6.555003397676761E-4	0.0	0.0	20.01293520670475	0.0
39	6.555003397676761E-4	0.0	0.0	21.029106400086818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAA	20	7.033766E-4	45.0	9
TTACGGG	400	0.0	40.5	3
AGACACG	1245	0.0	40.301205	24
CACGACC	1240	0.0	40.100807	27
CGATTAC	45	1.9288564E-8	40.0	10
AACACGT	1210	0.0	39.97934	41
ACGACCA	1240	0.0	39.919353	28
TCAAGCG	1325	0.0	39.73585	17
ACACGAC	1255	0.0	39.621513	26
ACACGTG	1230	0.0	39.512196	42
AAACACG	1230	0.0	39.512196	40
GCGAGAC	1330	0.0	39.248123	21
CGGGATA	155	0.0	39.193546	6
CGACCAA	1270	0.0	39.15354	29
TCTTGCG	500	0.0	39.15	1
CGAGACA	1320	0.0	39.034092	22
CGTTTTT	2610	0.0	38.96552	1
TACGGGA	295	0.0	38.898304	4
GTCAAGC	1360	0.0	38.878677	16
TATGGGA	870	0.0	38.793106	4
>>END_MODULE