##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1952577
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.249310526550296	33.0	31.0	34.0	30.0	34.0
2	32.44063614392672	34.0	31.0	34.0	31.0	34.0
3	32.49610079397637	34.0	31.0	34.0	30.0	34.0
4	35.990246223324355	37.0	35.0	37.0	35.0	37.0
5	35.26799916213292	37.0	35.0	37.0	35.0	37.0
6	34.95835401113503	37.0	35.0	37.0	33.0	37.0
7	35.76879119235759	37.0	35.0	37.0	35.0	37.0
8	36.053190219899136	37.0	35.0	37.0	35.0	37.0
9	37.933210828561435	39.0	38.0	39.0	35.0	39.0
10	37.2269590392594	39.0	37.0	39.0	34.0	39.0
11	37.14065924160737	39.0	37.0	39.0	34.0	39.0
12	37.118275489263674	39.0	37.0	39.0	34.0	39.0
13	37.166421605908496	39.0	37.0	39.0	33.0	39.0
14	38.36986505525774	40.0	38.0	41.0	34.0	41.0
15	38.433410308530725	40.0	38.0	41.0	34.0	41.0
16	38.428678612930504	40.0	38.0	41.0	34.0	41.0
17	38.37498393149156	40.0	38.0	41.0	34.0	41.0
18	38.31387187291462	40.0	38.0	41.0	34.0	41.0
19	38.31569664090072	40.0	37.0	41.0	34.0	41.0
20	38.22969439873562	40.0	37.0	41.0	34.0	41.0
21	38.13016388086103	40.0	37.0	41.0	34.0	41.0
22	38.174640487929544	40.0	37.0	41.0	34.0	41.0
23	38.087159686916316	40.0	37.0	41.0	34.0	41.0
24	38.053067305412284	40.0	37.0	41.0	34.0	41.0
25	37.96591069135814	40.0	37.0	41.0	33.0	41.0
26	37.96010144542315	40.0	37.0	41.0	34.0	41.0
27	37.93186798779254	40.0	37.0	41.0	33.0	41.0
28	37.86462812990217	40.0	37.0	41.0	33.0	41.0
29	37.80479028483896	40.0	37.0	41.0	33.0	41.0
30	37.7357031246399	40.0	36.0	41.0	33.0	41.0
31	37.66195289609578	40.0	36.0	41.0	33.0	41.0
32	37.61527560756887	40.0	36.0	41.0	33.0	41.0
33	37.501169480128056	40.0	36.0	41.0	33.0	41.0
34	37.39663634263847	40.0	36.0	41.0	32.0	41.0
35	37.26716231933491	40.0	36.0	41.0	32.0	41.0
36	37.1636729306962	40.0	36.0	41.0	32.0	41.0
37	37.12972087656466	40.0	36.0	41.0	31.0	41.0
38	37.03629408724982	39.0	36.0	41.0	31.0	41.0
39	36.986553667281754	39.0	36.0	41.0	31.0	41.0
40	36.982826797611565	39.0	36.0	41.0	31.0	41.0
41	36.92737187829212	39.0	36.0	41.0	31.0	41.0
42	36.86812351062212	39.0	35.0	41.0	31.0	41.0
43	36.73913704811641	39.0	35.0	41.0	31.0	41.0
44	36.66513996631119	39.0	35.0	41.0	31.0	41.0
45	36.67761681101437	39.0	35.0	41.0	31.0	41.0
46	36.491539642226655	39.0	35.0	41.0	30.0	41.0
47	36.4340894110706	39.0	35.0	41.0	30.0	41.0
48	36.339990177083926	39.0	35.0	40.0	30.0	41.0
49	36.391259858125956	39.0	35.0	40.0	30.0	41.0
50	36.211773978695845	39.0	35.0	40.0	30.0	41.0
51	35.166661801301565	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	7.0
10	5.0
11	9.0
12	11.0
13	10.0
14	14.0
15	17.0
16	52.0
17	132.0
18	243.0
19	505.0
20	996.0
21	1767.0
22	2725.0
23	4447.0
24	7129.0
25	11374.0
26	15667.0
27	17145.0
28	18858.0
29	21520.0
30	26936.0
31	35172.0
32	46872.0
33	65558.0
34	104100.0
35	136112.0
36	158101.0
37	244666.0
38	437021.0
39	595194.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.427335772161612	4.18134598533118	57.04717406791128	16.344144174595932
2	20.21974037387514	3.7987746449947943	54.7395570059465	21.241927975183565
3	18.90860129971827	3.9852461644278305	55.73352548964778	21.372627046206116
4	17.1586062931193	4.226209773033278	54.80797940362915	23.80720453021827
5	15.882190561498982	7.438118957664666	54.18342016729686	22.49627031353949
6	19.46125556124035	7.183685969874684	56.83729758160626	16.51776088727871
7	85.58894220304755	1.3467330609753163	9.464210630361825	3.600114105615297
8	89.16810963152798	1.456229383015369	6.337368513508046	3.0382924719486093
9	83.63992815648244	4.189489070085328	8.334626496163787	3.835956277268451
10	36.654790054374295	24.41010008824236	21.488166663849874	17.446943193533468
11	26.920116338561805	28.551140364758982	24.36538994364883	20.16335335303038
12	20.075264637450918	25.201618169219447	33.150651677244994	21.57246551608464
13	21.205412129713707	25.66049892014502	34.69128234123418	18.4428066089071
14	21.159114339664967	27.19544478911715	33.10701703441145	18.538423836806437
15	19.442613530734	26.814614737344545	34.71192173215192	19.030849999769536
16	19.975396616881177	24.93981031221816	33.60236241643735	21.482430654463304
17	20.823455361811597	25.009461854769366	31.892929190500553	22.274153592918484
18	21.53712760111381	24.515089545764393	32.74682637355659	21.20095647956521
19	22.872132571468374	26.76590987192823	30.278344976920245	20.08361257968316
20	23.90656040709278	26.309794696956896	30.973682471933245	18.809962424017083
21	23.365685450560978	27.065155433050787	30.377700853794753	19.191458262593486
22	22.30011927826662	25.494974077846866	30.084549802645427	22.120356841241087
23	20.467822779844276	27.217313324903447	29.202894431307957	23.111969463944316
24	20.15495419642862	26.35588762952754	31.302632367379108	22.18652580666473
25	19.643681145481075	27.886941206415933	30.37693263825191	22.092445009851083
26	19.78037229773781	28.042171960439973	30.99273421739578	21.184721524426436
27	20.53486238954981	26.95002553036321	31.25546393304848	21.259648147038504
28	19.647368580086727	26.59244680235402	32.32901954698841	21.43116507057084
29	20.013244035958632	25.21508754840398	32.24886905868501	22.522799356952376
30	22.322909672704327	25.64923175884997	31.136032023320976	20.891826545124726
31	21.8567564813065	27.34104724167088	29.586182772817665	21.216013504204955
32	21.013307029633147	26.680945232889663	30.33411742533073	21.971630312146463
33	22.24547354598564	26.364286786129306	29.220870675010513	22.169368992874546
34	21.12403249654175	25.9653780619151	30.35634446170369	22.554244979839464
35	20.646868215696486	25.633560161775947	30.775124361292793	22.944447261234767
36	23.07478783167066	25.975518507080643	29.590638422966165	21.359055238282536
37	22.53821488217878	27.01537506587448	29.855621570877872	20.590788481068863
38	21.20669248895178	26.84744314820875	30.186107897409425	21.759756465430044
39	22.251311984111254	25.421737529429056	30.781065228157455	21.545885258302235
40	22.975892884121855	25.157522597060193	29.24504385742534	22.62154066139261
41	20.51135499393878	25.4075511490712	29.68927729866735	24.391816558322667
42	21.21212121212121	25.437818841459258	30.107903555147892	23.24215639127164
43	21.50470890520579	24.82637048372484	31.438145589136816	22.23077502193255
44	21.406735816308398	25.216316693272532	29.764255135648938	23.612692354770132
45	22.37340704105395	24.860376825088075	29.07531943682631	23.690896697031665
46	23.23488395079938	25.07901096858152	29.25631101872039	22.42979406189871
47	21.483813442440425	25.21216832934117	31.102486611283446	22.201531616934954
48	21.74398243961698	24.222245780832203	31.430207361860763	22.60356441769006
49	22.890006386431878	23.447065083732934	31.443420669197682	22.219507860637506
50	22.72750319193558	23.071458897651667	30.240139057256126	23.960898853156625
51	21.664088023161188	23.11437653931189	29.172677953289423	26.048857484237498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	142.0
1	609.0
2	1076.0
3	5043.5
4	9011.0
5	6525.5
6	4040.0
7	4135.0
8	4230.0
9	4398.5
10	4567.0
11	4627.5
12	4688.0
13	4774.0
14	4860.0
15	4919.5
16	4979.0
17	5252.0
18	5525.0
19	5645.0
20	5765.0
21	6671.0
22	7577.0
23	8657.0
24	9737.0
25	11764.5
26	17386.5
27	20981.0
28	24932.5
29	28884.0
30	34794.5
31	40705.0
32	45509.5
33	50314.0
34	59116.5
35	67919.0
36	75140.5
37	82362.0
38	90873.0
39	99384.0
40	108479.0
41	117574.0
42	124576.0
43	131578.0
44	140786.0
45	149994.0
46	156779.0
47	163564.0
48	174507.5
49	185451.0
50	194950.5
51	204450.0
52	191103.0
53	177756.0
54	149259.5
55	120763.0
56	100311.0
57	79859.0
58	66443.5
59	53028.0
60	45006.0
61	36984.0
62	30243.0
63	23502.0
64	19290.0
65	15078.0
66	11644.5
67	8211.0
68	6804.5
69	5398.0
70	4309.0
71	3220.0
72	2563.5
73	1907.0
74	1754.0
75	1328.0
76	1055.0
77	792.5
78	530.0
79	402.5
80	275.0
81	226.0
82	177.0
83	111.5
84	46.0
85	38.0
86	30.0
87	16.5
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1952577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.858398041044985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9764410443978	21.192428282403924
2	12.197544623480452	7.283982849825751
3	4.5657423577874345	4.089772580150293
4	2.3137848492967303	2.7634363604656422
5	1.3285271969864836	1.9833846927988108
6	0.8311352023735751	1.4889819419036812
7	0.6206171004770276	1.297144269198561
8	0.4657648003271769	1.112559264134135
9	0.36334731583106444	0.9764071904906289
>10	5.747591049596298	43.149598178660774
>50	0.5210539607715061	9.47677786605187
>100	0.0645991055953155	3.3376907461595953
>500	0.002275823183158877	0.45575175090540426
>1k	0.0014005065742516169	1.1296308164716928
>5k	1.750633217814521E-4	0.2624532103791759
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	5021	0.25714734937469813	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	4802	0.24593140244917355	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4622	0.23671281593504379	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	3407	0.17448735696466772	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	3367	0.17243878218375	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5607184761471634E-4	0.0	0.0	0.26401007489077255	0.0
2	2.5607184761471634E-4	0.0	0.0	0.9342525288375311	0.0
3	2.5607184761471634E-4	0.0	0.0	1.361380370658878	0.0
4	2.5607184761471634E-4	0.0	0.0	1.835318146224195	0.0
5	2.5607184761471634E-4	0.0	0.0	2.612035274409153	0.0
6	2.5607184761471634E-4	0.0	0.0	3.2638917696971745	0.0
7	2.5607184761471634E-4	0.0	0.0	3.78878784293782	0.0
8	2.5607184761471634E-4	0.0	0.0	4.6471918905118725	0.0
9	2.5607184761471634E-4	0.0	0.0	5.098953844073755	0.0
10	2.5607184761471634E-4	0.0	0.0	5.983016290778801	0.0
11	2.5607184761471634E-4	0.0	0.0	7.5813655492203385	0.0
12	2.5607184761471634E-4	0.0	0.0	9.014702109058952	0.0
13	2.5607184761471634E-4	0.0	0.0	9.512249708974345	0.0
14	3.072862171376596E-4	0.0	0.0	9.66911932282312	0.0
15	3.072862171376596E-4	0.0	0.0	9.882171100038564	0.0
16	3.072862171376596E-4	0.0	0.0	10.440561370947215	0.0
17	3.072862171376596E-4	0.0	0.0	11.322114313545637	0.0
18	3.072862171376596E-4	0.0	0.0	12.327964530976244	0.0
19	3.072862171376596E-4	0.0	0.0	13.014083439475113	0.0
20	4.097149561835462E-4	0.0	0.0	13.727089891973531	0.0
21	4.097149561835462E-4	0.0	0.0	14.727562600604227	0.0
22	4.097149561835462E-4	0.0	0.0	15.79932571161086	0.0
23	4.097149561835462E-4	0.0	0.0	16.90755345371783	0.0
24	4.097149561835462E-4	0.0	0.0	17.73456309277432	0.0
25	4.097149561835462E-4	0.0	0.0	18.440092247322386	0.0
26	4.097149561835462E-4	0.0	0.0	19.056201112683393	0.0
27	4.097149561835462E-4	0.0	0.0	19.67353912291295	0.0
28	4.097149561835462E-4	0.0	0.0	20.338660139907415	0.0
29	4.097149561835462E-4	0.0	0.0	21.025598478318653	0.0
30	4.097149561835462E-4	0.0	0.0	21.803954466328346	0.0
31	4.097149561835462E-4	0.0	0.0	22.54564096575961	0.0
32	4.097149561835462E-4	0.0	0.0	23.242258820010683	0.0
33	4.609293257064894E-4	0.0	0.0	23.93073358950761	0.0
34	4.609293257064894E-4	0.0	0.0	24.603024618235285	0.0
35	4.609293257064894E-4	0.0	0.0	25.31198513554139	0.0
36	4.609293257064894E-4	0.0	0.0	25.99846254462692	0.0
37	4.609293257064894E-4	0.0	0.0	26.69236603729328	0.0
38	4.609293257064894E-4	0.0	0.0	27.370751575994188	0.0
39	4.609293257064894E-4	0.0	0.0	28.04212074607045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATC	20	7.034498E-4	45.000004	13
GACGTCG	95	0.0	42.63158	35
GCGCGAC	65	0.0	41.53846	9
CGTTTTT	4505	0.0	41.25416	1
AAACTCG	50	1.0822987E-9	40.5	10
ACGTCGG	100	0.0	40.5	36
TAGGGCG	270	0.0	40.0	5
GCTACGA	85	0.0	39.705883	10
ACAATCG	40	3.4598816E-7	39.375004	23
CGTAGAC	35	6.2505333E-6	38.571426	35
TACCTCG	35	6.2505333E-6	38.571426	1
TCGTGCG	170	0.0	38.38235	1
CCGAATC	135	0.0	38.333332	17
GCGGAAC	65	9.094947E-12	38.07692	5
TTCCGCG	190	0.0	37.894737	1
CGTATGG	125	0.0	37.8	2
TCAAGCG	365	0.0	37.602737	17
GTATGCG	30	1.1402143E-4	37.499996	1
GTCCGCG	30	1.1402143E-4	37.499996	1
TCGCGCA	30	1.1402143E-4	37.499996	35
>>END_MODULE