SRR2933130.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244561
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	5346	0.42954905384308195	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	4153	0.3336919604583463	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	4080	0.3278264383987607	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3232	0.25968996296686137	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3222	0.25888646679431543	TruSeq Adapter, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1671	0.1342642104324336	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTA	25	3.8909915E-5	45.000004	11
TATTGCG	80	0.0	45.0	1
TACGCGC	35	1.212029E-7	45.0	34
TACGAAC	20	7.033513E-4	45.0	44
CGAATAC	20	7.033513E-4	45.0	31
AATAGCG	20	7.033513E-4	45.0	36
TAAGCGT	20	7.033513E-4	45.0	14
TCGAATC	20	7.033513E-4	45.0	19
TAGTTCG	30	2.1656488E-6	44.999996	1
CGTTTAG	30	2.1656488E-6	44.999996	30
GCGTACT	30	2.1656488E-6	44.999996	9
TTTACGG	210	0.0	41.785713	2
TTACGGG	365	0.0	41.30137	3
CGTTTTT	2300	0.0	40.108696	1
AATTCGC	45	1.9284926E-8	40.0	13
ACACGAC	85	0.0	39.705883	26
ACGCGCA	40	3.4585355E-7	39.375	35
TGTTACG	70	0.0	38.571426	1
CGGGATC	365	0.0	38.219177	6
CGAATAT	125	0.0	37.800003	14