##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244561
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.112237969854434	33.0	31.0	34.0	30.0	34.0
2	32.295368407012596	34.0	31.0	34.0	30.0	34.0
3	32.36060908223864	34.0	31.0	34.0	30.0	34.0
4	35.87352327447188	37.0	35.0	37.0	35.0	37.0
5	35.12189519035226	37.0	35.0	37.0	35.0	37.0
6	34.805503305985	37.0	35.0	37.0	32.0	37.0
7	35.618022740548675	37.0	35.0	37.0	33.0	37.0
8	35.8947034335802	37.0	35.0	37.0	35.0	37.0
9	37.759680722760876	39.0	38.0	39.0	35.0	39.0
10	37.18556342356863	39.0	37.0	39.0	34.0	39.0
11	37.08165529853498	39.0	37.0	39.0	33.0	39.0
12	36.92740090682578	39.0	37.0	39.0	33.0	39.0
13	36.89729470873665	39.0	37.0	39.0	33.0	39.0
14	38.021775549772165	40.0	37.0	41.0	33.0	41.0
15	38.10177966367257	40.0	37.0	41.0	33.0	41.0
16	38.100371938378274	40.0	37.0	41.0	33.0	41.0
17	38.06445083848843	40.0	37.0	41.0	33.0	41.0
18	38.00975122955002	40.0	37.0	41.0	33.0	41.0
19	38.006653751804855	40.0	37.0	41.0	34.0	41.0
20	37.93386021255688	40.0	36.0	41.0	33.0	41.0
21	37.7989797205601	40.0	36.0	41.0	33.0	41.0
22	37.84850160016263	40.0	36.0	41.0	33.0	41.0
23	37.77567672456392	39.0	36.0	41.0	33.0	41.0
24	37.71758314779267	39.0	36.0	41.0	33.0	41.0
25	37.60162900814022	39.0	36.0	41.0	33.0	41.0
26	37.58050107628312	39.0	36.0	41.0	33.0	41.0
27	37.51447217131181	39.0	36.0	41.0	33.0	41.0
28	37.45110524916014	39.0	36.0	41.0	33.0	41.0
29	37.41094490346395	39.0	36.0	41.0	33.0	41.0
30	37.31147288079893	39.0	35.0	41.0	32.0	41.0
31	37.22154076819055	39.0	35.0	41.0	32.0	41.0
32	37.15492691800563	39.0	35.0	41.0	32.0	41.0
33	37.02417719983191	39.0	35.0	41.0	31.0	41.0
34	36.92286597442793	39.0	35.0	41.0	31.0	41.0
35	36.76624608998675	39.0	35.0	41.0	31.0	41.0
36	36.61212989961923	39.0	35.0	41.0	31.0	41.0
37	36.58696841697594	39.0	35.0	40.0	31.0	41.0
38	36.503158945202365	39.0	35.0	40.0	31.0	41.0
39	36.47744787117706	39.0	35.0	40.0	30.0	41.0
40	36.39195266443348	39.0	35.0	40.0	30.0	41.0
41	36.185394689372394	38.0	35.0	40.0	30.0	41.0
42	36.0558735168465	38.0	35.0	40.0	30.0	41.0
43	35.93844014074039	38.0	35.0	40.0	29.0	41.0
44	35.87643353760885	38.0	35.0	40.0	29.0	41.0
45	35.85632042141767	38.0	35.0	40.0	29.0	41.0
46	35.55899228724024	38.0	34.0	40.0	28.0	41.0
47	35.381916193742214	37.0	34.0	40.0	28.0	41.0
48	35.20092627038771	37.0	34.0	40.0	28.0	41.0
49	35.19964148000781	37.0	34.0	40.0	28.0	41.0
50	34.92789827095659	36.0	34.0	40.0	27.0	41.0
51	33.74197327411031	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	26.0
11	19.0
12	11.0
13	20.0
14	20.0
15	36.0
16	61.0
17	100.0
18	228.0
19	440.0
20	830.0
21	1342.0
22	2205.0
23	3108.0
24	4678.0
25	7197.0
26	9712.0
27	12163.0
28	14551.0
29	17930.0
30	22209.0
31	28585.0
32	37032.0
33	51664.0
34	84286.0
35	111064.0
36	118653.0
37	174861.0
38	265025.0
39	276396.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.224735468972593	4.6659826235917725	56.56235411522618	15.54692779220946
2	20.85329686532038	4.622834879126053	52.598305747970564	21.925562507582995
3	19.946953182688514	4.82443206881784	52.62353552778851	22.605079220705132
4	18.90321165455128	4.733235253233871	51.60164909554453	24.761903996670313
5	16.9603579093351	7.863495642238508	50.87424401053866	24.301902437887737
6	21.400799157293214	7.566362757631004	53.7949525977433	17.23788548733248
7	83.29796611013843	1.9120798418076734	10.065235854249007	4.724718193804884
8	85.66578898101419	2.28875884749723	7.292611611644588	4.752840559843993
9	79.85297627034753	4.971070120307481	9.533803485727095	5.642150123617886
10	39.94942795089996	24.679465289367094	18.411471996953143	16.959634762779807
11	29.54889314384751	25.66173936030456	24.31242823774809	20.476939258099843
12	24.109304405328466	22.75477055765045	31.89622686232334	21.239698174697743
13	23.047805611777967	24.553798487980902	32.53926484921189	19.859131051029237
14	21.155572125432183	25.668569077771195	31.96275634541015	21.213102451386472
15	19.108424577019527	24.548575762859354	35.1308614041417	21.21213825597942
16	20.591116064218628	22.933146707955657	32.443889853530685	24.031847374295033
17	21.132270736428346	22.573100073037804	29.936499697483693	26.358129493050157
18	22.231051752384978	22.357602399560967	32.17777192118345	23.233573926870598
19	23.86528261772625	24.82128236382146	29.290488774756724	22.022946243695568
20	25.608387214447504	24.680027736687876	28.31175008697846	21.399834961886157
21	24.98149950062713	25.424466940551728	28.71156978243734	20.8824637763838
22	23.47855990987987	23.85893499796314	27.276204219801198	25.386300872355793
23	21.91270656882226	24.731531841348072	26.932629256420537	26.42313233340913
24	22.676751079296235	24.309937399613197	28.711328733585578	24.30198278750499
25	21.28244417107719	25.108210847037633	28.155389731801012	25.453955250084164
26	21.72942909186452	25.94738224964465	27.779594571901256	24.543594086589568
27	21.342143936697358	26.12616014803614	28.492295676949542	24.039400238316965
28	21.15886645973962	23.72041225781621	30.5953665589714	24.525354723472777
29	22.683420097528366	23.1496889264568	29.06229586175366	25.104595114261173
30	23.84768605154749	24.59148245847331	27.926955769946186	23.633875720033007
31	25.602119944301645	26.22306178644518	24.907497503135644	23.267320766117532
32	24.92662071204224	26.2278024138632	25.85240900204972	22.993167872044843
33	24.81798802951402	25.62276979593608	25.135208318435176	24.424033856114725
34	23.661114240282316	24.50157123676541	26.790731832348918	25.04658269060335
35	23.46666816652619	24.6594582346707	26.818372100684503	25.055501498118616
36	25.388711360873433	26.15460391254426	25.799217555427173	22.65746717115513
37	24.558699814633435	27.08698087116662	26.73063031864248	21.62368899555747
38	23.43452831962435	26.48114475706695	26.42578387077853	23.65854305253017
39	23.920884552866433	24.201224367467724	27.471534139347124	24.406356940318716
40	24.499321447482288	23.24249273438586	27.697959360770586	24.56022645736127
41	21.759720897569505	24.343041441922093	27.919402905924258	25.977834754584144
42	22.09566264731098	25.780496094606853	27.229681791410787	24.894159466671383
43	22.860430304340245	24.826987186646537	27.74391934184022	24.568663167173003
44	23.513431643768364	23.744276094140826	27.011532580564552	25.730759681526255
45	24.376225833848242	23.615877405767975	25.648079925371274	26.359816835012506
46	24.422587563004143	23.735357286625565	27.456026663216992	24.386028487153304
47	22.490339967265566	24.53491632792607	29.27699003905795	23.69775366575041
48	22.255879784116647	24.017223743954695	29.11307681985857	24.613819652070088
49	23.918313365114287	22.219642106734824	29.765837110434923	24.096207417715966
50	23.607842444042518	22.331087025866953	27.7926112098965	26.268459320194026
51	22.580652937059735	22.448638515910428	26.298188678578228	28.67251986845161
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	611.0
2	1045.0
3	3419.5
4	5794.0
5	3995.5
6	2197.0
7	2181.0
8	2165.0
9	2227.5
10	2290.0
11	2357.5
12	2425.0
13	2441.5
14	2458.0
15	2469.0
16	2480.0
17	2569.5
18	2659.0
19	2871.0
20	3083.0
21	3113.0
22	3143.0
23	3505.5
24	3868.0
25	4667.0
26	7022.0
27	8578.0
28	9179.5
29	9781.0
30	12870.0
31	15959.0
32	18553.5
33	21148.0
34	25222.0
35	29296.0
36	31480.5
37	33665.0
38	38506.5
39	43348.0
40	47442.5
41	51537.0
42	58455.5
43	65374.0
44	70891.0
45	76408.0
46	82880.5
47	89353.0
48	102186.5
49	115020.0
50	128939.0
51	142858.0
52	136394.0
53	129930.0
54	110401.5
55	90873.0
56	80564.0
57	70255.0
58	62315.5
59	54376.0
60	49414.5
61	44453.0
62	38631.5
63	32810.0
64	29171.5
65	25533.0
66	22214.0
67	18895.0
68	15612.0
69	12329.0
70	10764.5
71	9200.0
72	7698.5
73	6197.0
74	4964.0
75	2919.5
76	2108.0
77	1648.0
78	1188.0
79	836.0
80	484.0
81	430.5
82	377.0
83	292.5
84	208.0
85	115.5
86	23.0
87	15.0
88	7.0
89	7.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1244561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.823959495069495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33505503019444	25.481298499001287
2	11.198604884381577	7.575623160212198
3	4.134300033870116	4.195151906582618
4	2.0312707664297895	2.7482248050895994
5	1.122144747871949	1.8977689249812888
6	0.7243099956303792	1.469941917244555
7	0.503878361303847	1.193021289822832
8	0.38603363757367093	1.0445748896820948
9	0.29877304547900174	0.9095118649650279
>10	3.325174180142168	28.032998887923693
>50	0.8688365286996761	18.868967214095786
>100	0.06763996686055886	4.203551091374163
>500	0.0018723866178018366	0.402471867555309
>1k	0.0018723866178018366	1.553680738127556
>5k	2.3404832722522957E-4	0.42321294334199094
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	5346	0.42954905384308195	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	4153	0.3336919604583463	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	4080	0.3278264383987607	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3232	0.25968996296686137	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3222	0.25888646679431543	TruSeq Adapter, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1671	0.1342642104324336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6069923450919642E-4	0.0	0.0	0.3557077555861063	0.0
2	1.6069923450919642E-4	0.0	0.0	1.5011718991676584	0.0
3	1.6069923450919642E-4	0.0	0.0	2.191937558705439	0.0
4	1.6069923450919642E-4	0.0	0.0	2.935010819075963	0.0
5	1.6069923450919642E-4	0.0	0.0	4.117757185063649	0.0
6	1.6069923450919642E-4	0.0	0.0	5.009637936589689	0.0
7	1.6069923450919642E-4	0.0	0.0	5.739855258199477	0.0
8	1.6069923450919642E-4	0.0	0.0	6.984872577559477	0.0
9	1.6069923450919642E-4	0.0	0.0	7.6541848892902795	0.0
10	1.6069923450919642E-4	0.0	0.0	8.877748860843301	0.0
11	1.6069923450919642E-4	0.0	0.0	11.028386716279877	0.0
12	1.6069923450919642E-4	0.0	0.0	12.7927839615736	0.0
13	1.6069923450919642E-4	0.0	0.0	13.392834903230938	0.0
14	1.6069923450919642E-4	0.0	0.0	13.599895866896038	0.0
15	1.6069923450919642E-4	0.0	0.0	13.899037491934907	0.0
16	2.4104885176379461E-4	0.0	0.0	14.678348429687254	0.0
17	2.4104885176379461E-4	0.0	0.0	15.801957477375556	0.0
18	3.2139846901839284E-4	0.0	0.0	17.0049519469114	0.0
19	3.2139846901839284E-4	0.0	0.0	17.81640273156559	0.0
20	3.2139846901839284E-4	0.0	0.0	18.60696261573358	0.0
21	4.0174808627299103E-4	0.0	0.0	19.755238995919044	0.0
22	4.0174808627299103E-4	0.0	0.0	20.880455035952437	0.0
23	4.0174808627299103E-4	0.0	0.0	22.111812920379155	0.0
24	4.0174808627299103E-4	0.0	0.0	23.03511037225174	0.0
25	4.0174808627299103E-4	0.0	0.0	23.84270437527771	0.0
26	4.0174808627299103E-4	0.0	0.0	24.54608492472446	0.0
27	4.8209770352758923E-4	0.0	0.0	25.224316043970525	0.0
28	4.8209770352758923E-4	0.0	0.0	25.921509672888675	0.0
29	5.624473207821875E-4	0.0	0.0	26.71914032337507	0.0
30	5.624473207821875E-4	0.0	0.0	27.522556146303796	0.0
31	5.624473207821875E-4	0.0	0.0	28.273503669165272	0.0
32	5.624473207821875E-4	0.0	0.0	29.00267644575075	0.0
33	5.624473207821875E-4	0.0	0.0	29.714654404243745	0.0
34	5.624473207821875E-4	0.0	0.0	30.42590921618145	0.0
35	5.624473207821875E-4	0.0	0.0	31.189873377038168	0.0
36	5.624473207821875E-4	0.0	0.0	31.947650617366286	0.0
37	5.624473207821875E-4	0.0	0.0	32.68606359993604	0.0
38	5.624473207821875E-4	0.0	0.0	33.41170099336232	0.0
39	5.624473207821875E-4	0.0	0.0	34.137820484492124	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTA	25	3.8909915E-5	45.000004	11
TATTGCG	80	0.0	45.0	1
TACGCGC	35	1.212029E-7	45.0	34
TACGAAC	20	7.033513E-4	45.0	44
CGAATAC	20	7.033513E-4	45.0	31
AATAGCG	20	7.033513E-4	45.0	36
TAAGCGT	20	7.033513E-4	45.0	14
TCGAATC	20	7.033513E-4	45.0	19
TAGTTCG	30	2.1656488E-6	44.999996	1
CGTTTAG	30	2.1656488E-6	44.999996	30
GCGTACT	30	2.1656488E-6	44.999996	9
TTTACGG	210	0.0	41.785713	2
TTACGGG	365	0.0	41.30137	3
CGTTTTT	2300	0.0	40.108696	1
AATTCGC	45	1.9284926E-8	40.0	13
ACACGAC	85	0.0	39.705883	26
ACGCGCA	40	3.4585355E-7	39.375	35
TGTTACG	70	0.0	38.571426	1
CGGGATC	365	0.0	38.219177	6
CGAATAT	125	0.0	37.800003	14
>>END_MODULE