##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	783066
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14835786510971	33.0	31.0	34.0	30.0	34.0
2	32.330210991155276	34.0	31.0	34.0	30.0	34.0
3	32.415631632582695	34.0	31.0	34.0	30.0	34.0
4	35.91836192607009	37.0	35.0	37.0	35.0	37.0
5	35.19737033659998	37.0	35.0	37.0	35.0	37.0
6	34.80960353278012	37.0	35.0	37.0	32.0	37.0
7	35.62306880901482	37.0	35.0	37.0	33.0	37.0
8	35.82684345891662	37.0	35.0	37.0	35.0	37.0
9	37.73396367611414	39.0	38.0	39.0	35.0	39.0
10	37.24352098035159	39.0	37.0	39.0	34.0	39.0
11	37.09128860147165	39.0	37.0	39.0	33.0	39.0
12	36.96833600232931	39.0	37.0	39.0	33.0	39.0
13	36.88871436124158	39.0	37.0	39.0	33.0	39.0
14	37.99708709099872	40.0	37.0	41.0	33.0	41.0
15	38.08566583148802	40.0	37.0	41.0	33.0	41.0
16	38.126137515867114	40.0	37.0	41.0	33.0	41.0
17	38.03795465516317	40.0	37.0	41.0	33.0	41.0
18	38.012227577241255	40.0	37.0	41.0	33.0	41.0
19	38.006192326061914	40.0	37.0	41.0	34.0	41.0
20	37.86150848076663	40.0	36.0	41.0	33.0	41.0
21	37.78018200253874	40.0	36.0	41.0	33.0	41.0
22	37.80511093573211	40.0	36.0	41.0	33.0	41.0
23	37.69942380335757	39.0	36.0	41.0	33.0	41.0
24	37.656143926565576	39.0	36.0	41.0	33.0	41.0
25	37.54362595234629	39.0	36.0	41.0	33.0	41.0
26	37.49182061282191	39.0	35.0	41.0	33.0	41.0
27	37.42883869303481	39.0	35.0	41.0	33.0	41.0
28	37.36863431690305	39.0	35.0	41.0	32.0	41.0
29	37.30463715702125	39.0	35.0	41.0	32.0	41.0
30	37.20188847427931	39.0	35.0	41.0	32.0	41.0
31	37.10126860315733	39.0	35.0	41.0	32.0	41.0
32	37.00883961249754	39.0	35.0	41.0	31.0	41.0
33	36.89210743411156	39.0	35.0	41.0	31.0	41.0
34	36.711404913506655	39.0	35.0	41.0	31.0	41.0
35	36.58955694666861	39.0	35.0	41.0	30.0	41.0
36	36.43712279680129	39.0	35.0	40.0	30.0	41.0
37	36.38263569098901	39.0	35.0	40.0	30.0	41.0
38	36.37410512013036	39.0	35.0	40.0	30.0	41.0
39	36.27078943537327	39.0	35.0	40.0	30.0	41.0
40	36.14869117034835	38.0	35.0	40.0	30.0	41.0
41	35.932575287395956	38.0	35.0	40.0	29.0	41.0
42	35.94142767020915	38.0	35.0	40.0	30.0	41.0
43	35.8260146654305	38.0	35.0	40.0	29.0	41.0
44	35.816339874289014	38.0	35.0	40.0	29.0	41.0
45	35.84301706369578	38.0	35.0	40.0	29.0	41.0
46	35.61364176199707	38.0	34.0	40.0	28.0	41.0
47	35.469717750483355	38.0	34.0	40.0	28.0	41.0
48	35.40495820275686	37.0	34.0	40.0	28.0	41.0
49	35.48193511147208	37.0	34.0	40.0	28.0	41.0
50	35.24442001057382	37.0	34.0	40.0	27.0	41.0
51	34.1902355612426	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	17.0
11	16.0
12	11.0
13	15.0
14	16.0
15	24.0
16	60.0
17	91.0
18	161.0
19	349.0
20	636.0
21	1113.0
22	1804.0
23	2508.0
24	3401.0
25	4875.0
26	6291.0
27	7892.0
28	9264.0
29	11235.0
30	13750.0
31	17675.0
32	23509.0
33	32627.0
34	53677.0
35	69518.0
36	71913.0
37	105940.0
38	162684.0
39	181932.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.049357270013	4.416230560386992	51.76856101529118	18.765851154308834
2	18.441868246099308	7.6301359016992185	52.36825503852805	21.559740813673432
3	20.040456360000306	7.70228818515936	51.343820316550584	20.91343513828975
4	17.982647695085728	4.643286772762449	53.65652448197215	23.717541050179676
5	19.797054143584322	7.655293423542843	48.617102517540026	23.930549915332808
6	21.41339299624808	9.31377431787359	52.24783096188571	17.025001723992613
7	81.99257789254035	1.8753208541808737	11.863112432413105	4.268988820865674
8	83.50943598623871	4.760007457864344	7.467186673920207	4.263369881976742
9	78.42135911915472	4.754771628445112	9.12949866294795	7.69437058945223
10	41.4182201755663	27.3462262440203	15.758952629791104	15.476600950622297
11	29.8347776560341	22.37614709360386	27.090053711947654	20.699021538414385
12	25.250234335292298	20.759680537783535	31.866151767539392	22.123933359384775
13	23.40581764500055	22.760533594869397	34.24117507336547	19.59247368676459
14	20.897983056345186	25.66488648466413	32.25820045819893	21.17893000079176
15	18.33689625140154	22.120613077314044	37.466318292455554	22.076172378828858
16	20.969496824022496	21.226307871877978	32.46444105605402	25.339754248045505
17	20.621888831848146	21.47430740193036	31.343845857181897	26.559957909039593
18	21.656156696881233	20.784454950157457	31.542168859329866	26.017219493631444
19	23.119251761665044	22.926036885779745	29.48257235022335	24.47213900233186
20	25.76423954047296	22.868059652698495	30.175361974597287	21.192338832231254
21	25.034671407007835	25.41944101774308	28.634112577994703	20.91177499725438
22	23.349883662424368	21.698937254331053	29.509645419415477	25.4415336638291
23	22.448299377064	24.945917713194035	28.384708313220088	24.221074596521877
24	23.213368988054647	22.62120945105521	28.894371611077485	25.271049949812657
25	21.146621102180404	26.09831099805125	27.26411311434796	25.490954785420385
26	20.444892256846806	24.50649115144828	28.569750187085123	26.47886640461979
27	21.340857603318238	25.192129399054487	28.16276022710729	25.30425277051998
28	19.19033644673629	24.164246691849726	30.755262008566326	25.89015485284765
29	21.635596488674008	22.17297137150636	29.269180375600524	26.92225176421911
30	22.75542546860673	24.30369853882048	29.52586372029944	23.415012272273348
31	25.05229444261403	23.714220768108945	25.72005424830091	25.513430540976113
32	25.88849471181229	24.882321541223856	25.661949312063097	23.56723443490076
33	24.437531446902305	23.090901660907253	26.425103375705238	26.0464635164852
34	22.987972916714554	23.03701092883614	28.29838097938105	25.676635175068256
35	23.07174618742226	23.720605925937278	27.28633346359055	25.921314423049907
36	23.475543568485925	24.19719410624392	27.663440884931795	24.663821440338364
37	22.635512204590675	25.658501326835797	28.172210260693227	23.533776207880305
38	23.80450689980155	25.23439914387804	26.095246122293652	24.86584783402676
39	25.060722850947432	22.88338403148649	28.372448810189688	23.68344430737639
40	25.381002367616524	22.223797227819876	28.43068144958407	23.964518954979532
41	21.53866979283994	23.326513984772674	29.018626782416806	26.116189439970576
42	23.564169559143163	22.959239706487065	28.22520707066837	25.2513836637014
43	22.692595515575952	22.425568215195142	28.74687446524303	26.134961803985874
44	22.3522666033259	22.772026878960393	28.46937550602376	26.40633101168995
45	23.021048034265313	22.357885542214834	26.54603826497383	28.075028158546022
46	24.92714534917874	22.991293198785286	27.86227469970603	24.219286752329943
47	21.235119389681074	23.43263530787954	31.330309322585837	24.00193597985355
48	22.277177147264727	23.45906986128883	29.349505661080933	24.914247330365512
49	23.637598874168972	21.355415763166835	30.79255133028378	24.214434032380414
50	23.116953104846846	21.752189470619335	29.339161705399036	25.791695719134783
51	21.590261868092856	21.760107066326466	27.855761838721126	28.79386922685955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	487.0
2	879.0
3	2417.0
4	3955.0
5	2759.0
6	1563.0
7	1585.5
8	1608.0
9	1631.5
10	1655.0
11	1629.5
12	1604.0
13	1623.0
14	1642.0
15	1787.0
16	1932.0
17	1849.5
18	1767.0
19	1741.0
20	1715.0
21	1847.0
22	1979.0
23	2455.5
24	2932.0
25	3551.0
26	4708.0
27	5246.0
28	6592.5
29	7939.0
30	9326.5
31	10714.0
32	12048.5
33	13383.0
34	15371.0
35	17359.0
36	18631.5
37	19904.0
38	22372.5
39	24841.0
40	28743.0
41	32645.0
42	36274.0
43	39903.0
44	42118.0
45	44333.0
46	48081.5
47	51830.0
48	66646.0
49	81462.0
50	80881.0
51	80300.0
52	76704.5
53	73109.0
54	63792.5
55	54476.0
56	48951.5
57	43427.0
58	40851.0
59	38275.0
60	35082.0
61	31889.0
62	27866.0
63	23843.0
64	21599.5
65	19356.0
66	16548.5
67	13741.0
68	11245.0
69	8749.0
70	7717.5
71	6686.0
72	5839.5
73	4993.0
74	3975.0
75	2428.5
76	1900.0
77	1506.5
78	1113.0
79	942.5
80	772.0
81	521.0
82	270.0
83	175.0
84	80.0
85	60.0
86	40.0
87	30.0
88	20.0
89	14.0
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	783066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.953968981382424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66911918987519	24.17928687441801
2	10.289926348883597	6.576079747458724
3	3.552183043867834	3.4051904039983607
4	1.6483179636960226	2.1068120433359256
5	0.9262912440393909	1.4799340839880417
6	0.6533892207279832	1.2527027335146963
7	0.4400587265175615	0.9843136027990166
8	0.3569248512007231	0.9124132499161954
9	0.29567766106526566	0.8503267329150483
>10	5.874945645528559	47.15459814841073
>50	0.24101256600932786	4.719639326600263
>100	0.04622700011260783	2.4468696829885435
>500	0.0023706153903901455	0.6256997135798577
>1k	0.0031608205205201937	2.638266124834198
>5k	3.951025650650242E-4	0.6678675312424227
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	5290	0.675549698237441	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	4195	0.5357147417969877	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	4120	0.5261370050544909	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2626	0.33534848914395465	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2517	0.32142884507819264	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2442	0.31185110833569585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2012	0.2569387510120475	No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1866	0.2382940901533204	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGGTGTT	1119	0.14289983219805227	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	957	0.12221192083425919	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTC	938	0.11978556085949332	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	916	0.1169760914150276	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTA	876	0.11186796515236264	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3633154804320453	0.0
2	0.0	0.0	0.0	1.33756286187882	0.0
3	0.0	0.0	0.0	1.813384823246061	0.0
4	0.0	0.0	0.0	2.4931487256502005	0.0
5	0.0	0.0	0.0	3.886390163792069	0.0
6	0.0	0.0	0.0	4.503707222635129	0.0
7	0.0	0.0	0.0	5.1866637039534345	0.0
8	0.0	0.0	0.0	6.191049030349932	0.0
9	0.0	0.0	0.0	6.667509507500006	0.0
10	0.0	0.0	0.0	8.07645843389957	0.0
11	0.0	0.0	0.0	9.485024250829431	0.0
12	0.0	0.0	0.0	11.682157059558198	0.0
13	0.0	0.0	0.0	12.144825595799077	0.0
14	0.0	0.0	0.0	12.331144501229781	0.0
15	0.0	0.0	0.0	12.669302459818201	0.0
16	0.0	0.0	0.0	13.256736980024673	0.0
17	0.0	0.0	0.0	14.077357464121798	0.0
18	0.0	0.0	0.0	14.977153905290232	0.0
19	0.0	0.0	0.0	15.775937149614464	0.0
20	0.0	0.0	0.0	16.47396260340763	0.0
21	0.0	0.0	0.0	17.273384363514698	0.0
22	0.0	0.0	0.0	18.178672040415496	0.0
23	0.0	0.0	0.0	19.02202368638148	0.0
24	0.0	0.0	0.0	19.655431342951935	0.0
25	1.27703156566624E-4	0.0	0.0	20.225115124395646	0.0
26	1.27703156566624E-4	0.0	0.0	20.725583794980246	0.0
27	1.27703156566624E-4	0.0	0.0	21.238439671751806	0.0
28	1.27703156566624E-4	0.0	0.0	21.772366569356862	0.0
29	1.27703156566624E-4	0.0	0.0	22.34511522655817	0.0
30	1.27703156566624E-4	0.0	0.0	23.000743232371217	0.0
31	1.27703156566624E-4	0.0	0.0	23.557018182375433	0.0
32	1.27703156566624E-4	0.0	0.0	24.08660317265722	0.0
33	2.55406313133248E-4	0.0	0.0	24.649390983646335	0.0
34	2.55406313133248E-4	0.0	0.0	25.18625505385242	0.0
35	2.55406313133248E-4	0.0	0.0	25.78122406029632	0.0
36	2.55406313133248E-4	0.0	0.0	26.30020968858308	0.0
37	2.55406313133248E-4	0.0	0.0	26.830177788334574	0.0
38	2.55406313133248E-4	0.0	0.0	27.35529316813653	0.0
39	2.55406313133248E-4	0.0	0.0	27.90377822559018	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGT	25	3.8896633E-5	45.0	15
CGCGATC	20	7.0319074E-4	45.0	21
TAACGAA	20	7.0319074E-4	45.0	19
CCGTCGA	20	7.0319074E-4	45.0	35
GTTACGG	35	1.211265E-7	45.0	2
AATTCGC	25	3.8896633E-5	45.0	13
CATACGT	20	7.0319074E-4	45.0	1
GCGATAT	20	7.0319074E-4	45.0	9
ACCGTTA	25	3.8896633E-5	45.0	23
CAATCGG	25	3.8896633E-5	45.0	20
TACGTCG	40	6.8102963E-9	45.0	38
ACGCGCA	25	3.8896633E-5	45.0	15
CGATGAA	215	0.0	42.906975	19
TACGAAT	75	0.0	42.0	12
AGCTACG	75	0.0	42.0	9
TTATGCG	55	6.002665E-11	40.909092	1
GGCGATA	50	1.0804797E-9	40.5	8
CCGATGA	230	0.0	40.108692	18
TCGATTG	45	1.9270374E-8	40.000004	1
TATGGGA	480	0.0	39.843746	4
>>END_MODULE