FastQCFastQC Report
Sat 14 Jan 2017
SRR2933122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011676
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC115281.1394952534210558No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.7491528908464765No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG60320.596238321359803No Hit
ACTCTGGACGAGGCGGAGCGCCAGTGGAAGGCCGAGTTCCACCGCTGGAGCT20080.19848251811844903No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT16760.16566568743352617Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT16290.16101993128234732No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA14300.14134960204650499No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT13430.1327500108730463No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10830.10705008322822722No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGCTTAACATCG10250.10131702244592143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA351.0194162E-746.00000410
AACCCGT351.0194162E-746.00000442
GTCTATA351.0194162E-746.00000435
TTCGTAC351.0194162E-746.00000433
ACGGAAC351.0194162E-746.00000442
TGCGTAA351.0194162E-746.00000421
AACCGAT253.4168632E-546.017
GGTACGA206.311094E-446.028
ACCGGTA1150.046.041
CGTATGG850.046.02
CTCCGTA206.311094E-446.044
GTACACC206.311094E-446.043
CGTGATA253.4168632E-546.015
CGTTGAT501.6370905E-1146.025
ACATTCG253.4168632E-546.031
TACGGTA253.4168632E-546.041
TACGGGT600.046.04
TCGATAT206.311094E-446.012
TGTGCGA206.311094E-446.031
AGTGCGT206.311094E-446.09
Produced by FastQC (version 0.11.5)