FastQCFastQC Report
Sat 14 Jan 2017
SRR2933121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963308
Sequences flagged as poor quality0
Sequence length52
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494435.132626325121352No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC70370.730503639542078No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC54350.5642016883488978No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT45220.4694241094229468No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT41520.43101479485273664No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC37790.3922940534076329No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG35870.3723627334144427No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT25970.2695918646995561No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCTT25660.2663737869923223Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG23330.24218629970891967No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT21630.2245387767982826No Hit
CGTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG18800.19516084159998673No Hit
GGGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT15650.16246101973615915No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC15330.15913913307062746No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT14220.14761633869956442No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGT11320.11751174079318348No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC10160.10546990163063112No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10140.10526228371403538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9710.10079849850722718No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG206.3109415E-446.00000441
GTTTGCG405.6097633E-946.0000041
CGGGTAT206.3109415E-446.0000046
CGAACGT206.3109415E-446.00000438
ACACGAT206.3109415E-446.00000441
CCCTCCG206.3109415E-446.00000444
ATAGGCG206.3109415E-446.00000413
CGCGGGT206.3109415E-446.0000044
TAGCGTA206.3109415E-446.00000430
GTTAGCG405.6097633E-946.0000041
TAACGGA206.3109415E-446.00000438
CCATCGG206.3109415E-446.00000427
CATAGCG206.3109415E-446.0000041
CCATAGT206.3109415E-446.00000426
TGTTACG206.3109415E-446.0000041
GTTCCGT206.3109415E-446.00000422
CGTACGA206.3109415E-446.00000433
ACGTCGA206.3109415E-446.00000429
TTACGGC206.3109415E-446.00000427
CAGTCGA206.3109415E-446.00000444
Produced by FastQC (version 0.11.5)