##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933121.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963308
Sequences flagged as poor quality	0
Sequence length	52
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48234105810395	33.0	31.0	34.0	31.0	34.0
2	32.82901315051884	34.0	31.0	34.0	31.0	34.0
3	32.76205533432713	34.0	31.0	34.0	31.0	34.0
4	36.29183708637321	37.0	37.0	37.0	35.0	37.0
5	36.23068011477118	37.0	37.0	37.0	35.0	37.0
6	36.32347909495198	37.0	37.0	37.0	35.0	37.0
7	36.53135030540596	37.0	37.0	37.0	35.0	37.0
8	36.46107579299663	37.0	37.0	37.0	35.0	37.0
9	38.310260062202325	39.0	39.0	39.0	37.0	39.0
10	38.033177343072	39.0	39.0	39.0	35.0	39.0
11	37.726236053266454	39.0	38.0	39.0	35.0	39.0
12	37.48996997844926	39.0	37.0	39.0	35.0	39.0
13	37.45504968296744	39.0	37.0	39.0	35.0	39.0
14	38.5471552193068	41.0	38.0	41.0	33.0	41.0
15	38.7464933333887	41.0	38.0	41.0	34.0	41.0
16	38.91129109277614	41.0	38.0	41.0	35.0	41.0
17	38.931282621965146	41.0	38.0	41.0	35.0	41.0
18	38.768208091285445	40.0	38.0	41.0	35.0	41.0
19	38.610466226793505	40.0	37.0	41.0	35.0	41.0
20	38.3867828358116	40.0	35.0	41.0	35.0	41.0
21	38.32165724773385	40.0	35.0	41.0	35.0	41.0
22	38.260675713271354	40.0	35.0	41.0	35.0	41.0
23	38.23132061604388	40.0	35.0	41.0	35.0	41.0
24	38.14444082266523	40.0	35.0	41.0	35.0	41.0
25	37.99713591084056	40.0	35.0	41.0	34.0	41.0
26	37.871014255046155	40.0	35.0	41.0	34.0	41.0
27	37.74710269197391	40.0	35.0	41.0	34.0	41.0
28	37.68044592176126	39.0	35.0	41.0	34.0	41.0
29	37.57421821473506	39.0	35.0	41.0	34.0	41.0
30	37.49342889293975	39.0	35.0	41.0	34.0	41.0
31	37.33994838618593	39.0	35.0	41.0	34.0	41.0
32	37.16890651795687	39.0	35.0	41.0	33.0	41.0
33	36.954098792909434	39.0	35.0	41.0	33.0	41.0
34	36.72772052137011	39.0	35.0	41.0	33.0	41.0
35	36.60963056467921	39.0	35.0	41.0	33.0	41.0
36	36.50329904869471	39.0	35.0	41.0	33.0	41.0
37	36.386645807986646	39.0	35.0	41.0	32.0	41.0
38	36.357303167834175	39.0	35.0	41.0	32.0	41.0
39	36.29361118147052	39.0	35.0	41.0	32.0	41.0
40	36.21486066761617	38.0	35.0	41.0	31.0	41.0
41	36.09528935709036	38.0	35.0	41.0	31.0	41.0
42	36.06703463482085	38.0	35.0	41.0	31.0	41.0
43	35.99704974940517	38.0	35.0	41.0	31.0	41.0
44	35.94671693788487	38.0	35.0	41.0	31.0	41.0
45	35.876918908594135	38.0	35.0	41.0	31.0	41.0
46	35.81725990026035	38.0	35.0	41.0	31.0	41.0
47	35.72540246733132	37.0	35.0	41.0	31.0	41.0
48	35.62568669625914	37.0	35.0	41.0	30.0	41.0
49	35.580963720845254	37.0	35.0	41.0	30.0	41.0
50	35.481416120285516	37.0	35.0	40.0	30.0	41.0
51	35.412944769481825	37.0	35.0	40.0	30.0	41.0
52	35.21410182413101	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	0.0
14	7.0
15	22.0
16	34.0
17	82.0
18	174.0
19	319.0
20	579.0
21	967.0
22	1497.0
23	2817.0
24	4820.0
25	8326.0
26	10806.0
27	11510.0
28	11125.0
29	11275.0
30	12580.0
31	14775.0
32	18971.0
33	26064.0
34	58078.0
35	95867.0
36	109379.0
37	90968.0
38	103371.0
39	364232.0
40	4660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.2801222454293	22.677793602876754	25.10661180017191	19.935472351522048
2	40.841039418337644	22.32972216570401	24.41098797061791	12.418250445340432
3	27.621799050770885	22.81710522491249	36.92453503967578	12.636560684640841
4	24.525904487453648	26.13743475606971	35.71474543967246	13.621915316804179
5	22.915931353212056	30.55232594351962	32.799997508585	13.73174519468332
6	24.064058431986446	35.89205114044521	29.696732509228617	10.34715791833972
7	79.09785862880823	1.486336664908835	15.475009031379372	3.940795674903561
8	76.32813181246289	1.9889796409870986	16.343786203374204	5.339102343175807
9	70.98944470512028	3.8271248655673986	19.277115937996985	5.906314491315342
10	40.63965003923979	26.095703554833964	22.377889522354224	10.886756883572025
11	24.80411249569193	22.342075431741456	36.83536314449792	16.018448928068697
12	24.451162037479186	19.367741158591024	36.316629779883485	19.864467024046306
13	23.02482695046652	21.99815635290063	39.059573884987984	15.917442811644875
14	14.787482300572611	25.52859521565273	39.759350072873886	19.92457241090077
15	11.570338873963468	31.159193113728946	40.358431571210865	16.91203644109672
16	19.230920951554438	30.62810648307706	36.187802862635834	13.953169702732668
17	18.54007233408214	31.75827461206592	31.482869445701688	18.21878360815025
18	15.829412815008284	31.538511047349342	35.302935302104835	17.329140835537544
19	15.917754238519768	34.17048337603342	30.650321600152807	19.261440785294006
20	13.878323443799905	39.33352572593605	28.709509315815918	18.078641514448133
21	16.59386198391376	35.29172393460866	33.27326254946497	14.841151532012606
22	15.296976667898532	31.33836737575106	33.60171409351941	19.762941862830996
23	14.609449937091771	32.31302968520971	33.036785742462435	20.04073463523608
24	15.745638985661905	33.013532535803705	33.775594098668336	17.465234379866047
25	18.241310152100887	31.654984698559545	31.801043902884647	18.302661246454925
26	15.284000548111301	35.0806803223891	29.290631864367366	20.344687265132233
27	18.333284889152797	32.52043998388885	31.506330270277005	17.639944856681353
28	16.37856220440399	33.966602581936414	28.916296760745265	20.73853845291433
29	16.60320479016057	32.32206106458163	29.469287081597994	21.605447063659806
30	19.8162996673961	31.70284062833486	29.408455032035448	19.072404672233596
31	17.592088926906037	32.399087311638645	31.38238237406935	18.626441387385967
32	19.71799258388802	31.790455389138263	30.687173780348548	17.80437824662517
33	19.874328875084604	32.56310546574927	29.448732907855018	18.113832751311108
34	15.400058963488314	31.021957670859162	34.051310691907474	19.526672673745054
35	16.900928882558848	32.65186212509394	30.798768410518758	19.64844058182845
36	18.74675597005319	32.96339280894584	27.670381643254288	20.61946957774668
37	15.659373741316381	34.59693057672105	30.8401881848796	18.90350749708297
38	16.818296951753748	32.74238353672968	30.361109842334955	20.07820966918161
39	16.07367529388316	34.62246758046233	27.789450518421937	21.514406607232576
40	20.019972843576507	31.549618605887215	30.232075307170707	18.198333243365568
41	15.921179934143598	31.917932789928038	29.32385073102268	22.83703654490568
42	15.93706270476317	34.31280545785979	30.446856041888992	19.303275795488048
43	16.85764054694864	33.838917563230034	31.970979167618246	17.332462722203076
44	19.36649545109145	32.74331781735437	28.536355973375077	19.353830758179107
45	17.40762040801073	31.96070208074676	27.641730370764073	22.989947140478435
46	19.015205936211473	31.167809257267663	27.918485053586185	21.89849975293468
47	17.141973283726493	30.774373305318758	30.406681975027716	21.676971435927033
48	15.941734107886575	32.328497219996095	29.86334588729669	21.86642278482064
49	19.590722801014838	30.02300406515881	29.835109850639668	20.551163283186686
50	16.525763307270363	29.946289244976686	31.737720438322945	21.790227009430005
51	15.138979433369181	30.91129732131364	30.21120970655284	23.73851353876434
52	15.513625963866179	31.31438750638425	31.826165670792726	21.34582085895684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1247.0
1	1673.5
2	2100.0
3	27534.5
4	52969.0
5	28860.5
6	4752.0
7	5780.0
8	6808.0
9	10553.0
10	14298.0
11	18352.0
12	22406.0
13	23550.5
14	23195.5
15	21696.0
16	19286.5
17	16877.0
18	14886.5
19	12896.0
20	12002.5
21	11109.0
22	10442.0
23	9775.0
24	9229.0
25	8683.0
26	9077.5
27	9472.0
28	10210.0
29	10948.0
30	11040.0
31	11132.0
32	12156.5
33	13181.0
34	15064.0
35	16947.0
36	18565.5
37	20184.0
38	21634.0
39	26481.0
40	29878.0
41	35988.0
42	42098.0
43	55658.0
44	69218.0
45	83011.5
46	96805.0
47	94860.5
48	92916.0
49	87350.0
50	81784.0
51	66628.0
52	51472.0
53	45159.0
54	38846.0
55	35260.5
56	31675.0
57	30484.5
58	29294.0
59	29525.5
60	29757.0
61	23383.0
62	17009.0
63	14646.5
64	10406.0
65	8528.0
66	6988.5
67	5449.0
68	4792.0
69	4135.0
70	3359.0
71	2583.0
72	2191.0
73	1799.0
74	1388.5
75	978.0
76	845.0
77	712.0
78	543.0
79	374.0
80	293.5
81	213.0
82	181.0
83	149.0
84	87.0
85	25.0
86	41.5
87	58.0
88	32.0
89	4.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	963308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.258392370586417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38000158310349	20.006889166197624
2	11.940262281572934	6.9870575768394865
3	5.792346011016141	5.0842419700953405
4	3.3276435260344837	3.8944599981663455
5	2.0897566565632024	3.0571460108385495
6	1.4062606656722882	2.4686955798937116
7	0.9910496804684581	2.0297564286923304
8	0.7077217029579896	1.6565439419459576
9	0.5567662116008548	1.466107584993248
>10	4.081827884750385	24.25270860751651
>50	0.528038026504928	10.678311585561872
>100	0.18978031468464968	7.877494580892547
>500	0.0021363637675565587	0.4170229952299479
>1k	0.005340909418891398	3.6734069035231562
>5k	7.121212558521864E-4	1.2993032217106948
>10k+	3.560606279260932E-4	5.150853847902652
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49443	5.132626325121352	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	7037	0.730503639542078	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	5435	0.5642016883488978	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	4522	0.4694241094229468	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	4152	0.43101479485273664	No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	3779	0.3922940534076329	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3587	0.3723627334144427	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	2597	0.2695918646995561	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCTT	2566	0.2663737869923223	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	2333	0.24218629970891967	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	2163	0.2245387767982826	No Hit
CGTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	1880	0.19516084159998673	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1565	0.16246101973615915	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC	1533	0.15913913307062746	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	1422	0.14761633869956442	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGT	1132	0.11751174079318348	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	1016	0.10546990163063112	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1014	0.10526228371403538	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	971	0.10079849850722718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27218708865700275	0.0
2	0.0	0.0	0.0	0.8046232357667538	0.0
3	0.0	0.0	0.0	1.4910080680322388	0.0
4	0.0	0.0	0.0	2.1601606132202784	0.0
5	0.0	0.0	0.0	4.119139465259294	0.0
6	0.0	0.0	0.0	6.891150078687191	0.0
7	0.0	0.0	0.0	9.265676190792561	0.0
8	0.0	0.0	0.0	12.7163897735719	0.0
9	0.0	0.0	0.0	14.81032027139814	0.0
10	0.0	0.0	0.0	16.43108953730271	0.0
11	1.0380895829786528E-4	0.0	0.0	18.441661441615764	0.0
12	1.0380895829786528E-4	0.0	0.0	19.584494263516966	0.0
13	1.0380895829786528E-4	0.0	0.0	20.140287426243734	0.0
14	1.0380895829786528E-4	0.0	0.0	20.598915404003705	0.0
15	1.0380895829786528E-4	0.0	0.0	20.974288597208783	0.0
16	1.0380895829786528E-4	0.0	0.0	21.503402857653004	0.0
17	1.0380895829786528E-4	0.0	0.0	22.12646422535679	0.0
18	1.0380895829786528E-4	0.0	0.0	22.781913988049514	0.0
19	1.0380895829786528E-4	0.0	0.0	23.207634526029057	0.0
20	1.0380895829786528E-4	0.0	0.0	23.695432821070728	0.0
21	1.0380895829786528E-4	0.0	0.0	24.164234076743888	0.0
22	1.0380895829786528E-4	0.0	0.0	24.669057040946406	0.0
23	1.0380895829786528E-4	0.0	0.0	25.19983224472339	0.0
24	1.0380895829786528E-4	0.0	0.0	25.581745402301237	0.0
25	1.0380895829786528E-4	0.0	0.0	25.94144344280334	0.0
26	1.0380895829786528E-4	0.0	0.0	26.272386401856934	0.0
27	1.0380895829786528E-4	0.0	0.0	26.661047141724143	0.0
28	1.0380895829786528E-4	0.0	0.0	26.98835678723731	0.0
29	1.0380895829786528E-4	0.0	0.0	27.391135545433027	0.0
30	1.0380895829786528E-4	0.0	0.0	28.15060188434021	0.0
31	1.0380895829786528E-4	0.0	0.0	28.58307000460912	0.0
32	1.0380895829786528E-4	0.0	0.0	28.948373728859305	0.0
33	1.0380895829786528E-4	0.0	0.0	29.303400366238005	0.0
34	2.0761791659573055E-4	0.0	0.0	29.6879087477733	0.0
35	2.0761791659573055E-4	0.0	0.0	30.051447719732423	6.228537497871917E-4
36	2.0761791659573055E-4	0.0	0.0	30.391733485032823	6.228537497871917E-4
37	2.0761791659573055E-4	0.0	0.0	30.72703642033493	6.228537497871917E-4
38	2.0761791659573055E-4	0.0	0.0	31.109987667495755	6.228537497871917E-4
39	2.0761791659573055E-4	0.0	0.0	32.07593002445739	6.228537497871917E-4
40	2.0761791659573055E-4	0.0	0.0	32.50704862826843	6.228537497871917E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	6.3109415E-4	46.000004	41
GTTTGCG	40	5.6097633E-9	46.000004	1
CGGGTAT	20	6.3109415E-4	46.000004	6
CGAACGT	20	6.3109415E-4	46.000004	38
ACACGAT	20	6.3109415E-4	46.000004	41
CCCTCCG	20	6.3109415E-4	46.000004	44
ATAGGCG	20	6.3109415E-4	46.000004	13
CGCGGGT	20	6.3109415E-4	46.000004	4
TAGCGTA	20	6.3109415E-4	46.000004	30
GTTAGCG	40	5.6097633E-9	46.000004	1
TAACGGA	20	6.3109415E-4	46.000004	38
CCATCGG	20	6.3109415E-4	46.000004	27
CATAGCG	20	6.3109415E-4	46.000004	1
CCATAGT	20	6.3109415E-4	46.000004	26
TGTTACG	20	6.3109415E-4	46.000004	1
GTTCCGT	20	6.3109415E-4	46.000004	22
CGTACGA	20	6.3109415E-4	46.000004	33
ACGTCGA	20	6.3109415E-4	46.000004	29
TTACGGC	20	6.3109415E-4	46.000004	27
CAGTCGA	20	6.3109415E-4	46.000004	44
>>END_MODULE