FastQCFastQC Report
Sat 14 Jan 2017
SRR2933120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872970
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167831.9225173831861346No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC97271.1142421847257065No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG49940.5720700596813179No Hit
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT24190.27710001489169156RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT22490.257626264361891RNA PCR Primer, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT19270.22074068982897466RNA PCR Primer, Index 16 (95% over 24bp)
GGGGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT14800.16953618108296964RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG14670.16804701192480842No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC14580.16701604866146602RNA PCR Primer, Index 16 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14020.16060116613400233No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA11170.1279539961281602No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAACCCATGTCG9810.11237499570431973No Hit
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT9720.11134403244097736RNA PCR Primer, Index 14 (95% over 21bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG9420.10790748822983609No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC9140.10470004696610423No Hit
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT9040.10355453222905713RNA PCR Primer, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG700.046.0000042
TTCGTCA351.01919795E-746.00000428
GAGCGAC700.046.00000418
TCTCGCG351.01919795E-746.00000432
TCGTCAC351.01919795E-746.00000429
AGCGATA351.01919795E-746.00000434
CGATACG351.01919795E-746.00000436
ACAACGC301.8608862E-646.018
CTATGCG301.8608862E-646.01
AACCGAT206.310617E-446.033
GCGACTA301.8608862E-646.040
CGTATTG253.416476E-546.019
CGTATCG206.310617E-446.025
CAACGTT405.6079443E-946.014
CGCGGGT206.310617E-446.04
ACGTTAG253.416476E-546.01
TAGGTTA206.310617E-446.039
TTTCGCG206.310617E-446.01
TTTCGCA405.6079443E-946.014
TAGCGTA206.310617E-446.016
Produced by FastQC (version 0.11.5)