##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872970
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40399670091756	33.0	31.0	34.0	31.0	34.0
2	32.77224532343609	34.0	31.0	34.0	31.0	34.0
3	32.8002966883169	34.0	31.0	34.0	31.0	34.0
4	36.27469099739968	37.0	37.0	37.0	35.0	37.0
5	36.183371708077026	37.0	37.0	37.0	35.0	37.0
6	36.2656288303149	37.0	37.0	37.0	35.0	37.0
7	36.498310365762855	37.0	37.0	37.0	35.0	37.0
8	36.43288543707115	37.0	37.0	37.0	35.0	37.0
9	38.25204760759247	39.0	39.0	39.0	37.0	39.0
10	37.954064859044415	39.0	38.0	39.0	35.0	39.0
11	37.63392785548186	39.0	37.0	39.0	35.0	39.0
12	37.29792890935542	39.0	37.0	39.0	35.0	39.0
13	37.206417173556936	39.0	37.0	39.0	34.0	39.0
14	38.073219010962575	40.0	37.0	41.0	33.0	41.0
15	38.338985303045924	40.0	37.0	41.0	33.0	41.0
16	38.5588725843958	41.0	37.0	41.0	34.0	41.0
17	38.596689462409934	40.0	37.0	41.0	35.0	41.0
18	38.560296459213944	40.0	37.0	41.0	35.0	41.0
19	38.49728856661741	40.0	37.0	41.0	35.0	41.0
20	38.33113509055294	40.0	35.0	41.0	34.0	41.0
21	38.285639827256375	40.0	35.0	41.0	34.0	41.0
22	38.2501036690837	40.0	35.0	41.0	34.0	41.0
23	38.22705934911853	40.0	35.0	41.0	34.0	41.0
24	38.14284797873925	40.0	35.0	41.0	34.0	41.0
25	38.00239412580043	40.0	35.0	41.0	34.0	41.0
26	37.88705912001558	40.0	35.0	41.0	34.0	41.0
27	37.796975841094195	40.0	35.0	41.0	34.0	41.0
28	37.771036805388505	40.0	35.0	41.0	34.0	41.0
29	37.70988350115124	40.0	35.0	41.0	34.0	41.0
30	37.666640319827714	40.0	35.0	41.0	34.0	41.0
31	37.5452707424081	40.0	35.0	41.0	34.0	41.0
32	37.50131505091813	40.0	35.0	41.0	34.0	41.0
33	37.36881107025442	40.0	35.0	41.0	33.0	41.0
34	37.20767265770874	40.0	35.0	41.0	33.0	41.0
35	37.16862778789649	40.0	35.0	41.0	33.0	41.0
36	37.0842892653814	40.0	35.0	41.0	33.0	41.0
37	36.957752270982965	39.0	35.0	41.0	33.0	41.0
38	36.92297100702201	39.0	35.0	41.0	33.0	41.0
39	36.883313286825434	39.0	35.0	41.0	33.0	41.0
40	36.82846260467141	39.0	35.0	41.0	33.0	41.0
41	36.7300857990538	39.0	35.0	41.0	33.0	41.0
42	36.733131722739614	39.0	35.0	41.0	32.0	41.0
43	36.683477095432835	39.0	35.0	41.0	32.0	41.0
44	36.64684124311259	39.0	35.0	41.0	32.0	41.0
45	36.58772924613675	39.0	35.0	41.0	32.0	41.0
46	36.535847738181154	39.0	35.0	41.0	32.0	41.0
47	36.449852801356286	38.0	35.0	41.0	32.0	41.0
48	36.34750449614534	38.0	35.0	41.0	31.0	41.0
49	36.30261864668889	38.0	35.0	41.0	31.0	41.0
50	36.16998293183042	38.0	35.0	41.0	31.0	41.0
51	36.10864634523523	38.0	35.0	41.0	31.0	41.0
52	35.855296287386736	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	4.0
14	9.0
15	13.0
16	46.0
17	87.0
18	162.0
19	308.0
20	548.0
21	837.0
22	1340.0
23	2074.0
24	3337.0
25	5071.0
26	6646.0
27	7629.0
28	8467.0
29	9708.0
30	11609.0
31	14423.0
32	18224.0
33	25076.0
34	59791.0
35	95377.0
36	68208.0
37	67840.0
38	96758.0
39	366497.0
40	2874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.908908668110016	23.7436567121436	26.890271143338257	13.457163476408123
2	35.59331935805354	23.94228896754757	26.77033575036943	13.694055924029463
3	30.763829226663002	23.91777495217476	31.205310606320953	14.11308521484129
4	27.09944213432306	27.791905792868025	30.749281189502504	14.359370883306413
5	24.45456315795503	32.048867658682425	28.244269562527926	15.252299620834622
6	26.052098010240904	39.09492880625909	24.891233375717377	9.961739807782626
7	85.55712109236285	1.5828722636516717	9.581543466556697	3.2784631774287774
8	82.78188253891886	1.8883810440221316	10.402762981545758	4.926973435513248
9	77.11696850979988	3.741480234143212	13.359451069338007	5.7821001867189015
10	45.94350321316883	25.063747895116673	17.423622804907385	11.569126086807106
11	29.271338075764348	23.10560500360837	30.947798893432765	16.67525802719452
12	29.465961029588644	18.858609116006278	32.36835171884486	19.307078135560214
13	27.00906102157004	22.252769281876812	34.400838516787516	16.337331179765627
14	16.396210637249848	25.356312358958498	37.52660457976792	20.720872424023735
15	13.495881874520316	30.435181048604193	38.84005177726612	17.228885299609377
16	24.05603858093634	28.5186203420507	31.969254384457656	15.4560866925553
17	21.96936893593136	27.904968097414574	27.787323733919838	22.338339232734228
18	19.270879869869525	27.39452673058639	34.05752775009451	19.27706564944958
19	19.498837302541897	30.741491689290584	28.393186478344045	21.366484529823477
20	16.285210259229984	37.657995120107216	26.91661798229034	19.140176638372452
21	19.11096601257775	31.982771458354815	32.51944511266138	16.386817416406064
22	17.398650583639757	27.200820188551727	33.31615061227763	22.084378615530888
23	17.768766395179675	28.771664547464404	32.57064962140738	20.888919435948544
24	18.61301075638338	31.119855206937235	30.898885414160855	19.36824862251853
25	20.955817496592093	29.33663241577603	28.886903330011343	20.820646757620537
26	16.19173625668694	32.894257534623186	26.53905632495962	24.374949883730253
27	20.05521381032567	30.152926217395787	29.769636986379826	20.022222985898715
28	15.942472249905496	32.32814415157451	26.324043208815883	25.40534038970411
29	17.962931143109156	32.27109751766957	25.92242574200717	23.843545597214106
30	22.30947226136064	31.301075638338087	24.493167004593513	21.896285095707757
31	19.080724423519708	31.381719875826203	26.430690630834963	23.106865069819126
32	22.48725614855035	30.872538575208768	22.426314764539445	24.213890511701432
33	21.674857097036554	30.868071067734288	24.076085088834667	23.38098674639449
34	15.637994432798378	29.669748101309324	28.68861472902849	26.003642736863807
35	17.083290376530694	33.25016896342372	25.42882344181358	24.237717218232014
36	22.338682887155343	30.907362223215003	23.366438709233993	23.38751618039566
37	17.81538884497749	33.37503006976185	25.728375545551398	23.081205539709266
38	19.68670171941762	29.018866627719163	28.31208403496111	22.982347617902104
39	19.3262082316689	29.895987261876122	25.704090633125997	25.07371387332898
40	23.25635474300377	28.460886399303526	28.57841621132456	19.704342646368143
41	18.615874543226	27.85571096372155	27.07973928084585	26.448675212206606
42	18.926881794334285	29.247511369233763	29.865287466923263	21.96031936950869
43	18.79423118778423	29.5718065912918	30.01512079452902	21.61884142639495
44	21.427311362360676	28.055145079441445	26.68740048340722	23.83014307479066
45	18.296619586010973	28.64199227923067	25.146568610605176	27.914819524153177
46	20.904498436372386	27.990194393850874	27.067940479054265	24.037366690722475
47	17.29188861014697	28.035327674490535	30.748135674765454	23.924648040597045
48	17.08729967811036	30.663023929802858	28.326288417700496	23.92338797438629
49	23.230809764367617	26.895998717023495	27.714010790748823	22.159180727860065
50	18.932494816545812	26.59644661328568	29.122535711421925	25.34852285874658
51	18.315406027698547	27.18214829833786	26.859800451332806	27.64264522263079
52	18.172102134093958	28.826878357790076	29.689336403312826	23.311683104803144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	872.0
1	1664.0
2	2456.0
3	10617.0
4	18778.0
5	10531.0
6	2284.0
7	2884.5
8	3485.0
9	5408.0
10	7331.0
11	9523.5
12	11716.0
13	12260.0
14	11900.0
15	10996.0
16	9619.5
17	8243.0
18	7316.5
19	6390.0
20	5723.5
21	5057.0
22	5139.5
23	5222.0
24	4774.5
25	4327.0
26	5380.0
27	6433.0
28	7554.5
29	8676.0
30	9599.5
31	10523.0
32	12008.5
33	13494.0
34	15268.5
35	17043.0
36	17217.0
37	17391.0
38	20029.0
39	25010.0
40	27353.0
41	30535.5
42	33718.0
43	41020.5
44	48323.0
45	58487.5
46	68652.0
47	79810.5
48	90969.0
49	94982.0
50	98995.0
51	88261.5
52	77528.0
53	66813.5
54	56099.0
55	49215.0
56	42331.0
57	39779.5
58	37228.0
59	37622.0
60	38016.0
61	28820.5
62	19625.0
63	16621.5
64	11312.5
65	9007.0
66	7165.5
67	5324.0
68	4492.5
69	3661.0
70	3008.0
71	2355.0
72	2041.0
73	1727.0
74	1469.5
75	1212.0
76	883.0
77	554.0
78	370.5
79	187.0
80	158.5
81	130.0
82	108.5
83	87.0
84	61.0
85	35.0
86	19.5
87	4.0
88	5.0
89	19.0
90	32.0
91	16.5
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	872970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.572042296518475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.11493073293448	17.674099320235452
2	12.56526164734848	6.426388046256334
3	5.369619863861519	4.119364388246776
4	2.74702536917308	2.809881957204131
5	1.7280799913008844	2.209526731465675
6	1.118574080432414	1.7162534217964167
7	0.7655921660195032	1.370442867593375
8	0.6231604501589123	1.2748388311184962
9	0.49342732913597986	1.135615007783108
>10	4.010043760911974	22.462932951579457
>50	1.1029268190649761	20.381559709404574
>100	0.34789103973266683	11.732764216102613
>500	0.008528941619252477	1.5181078833573727
>1k	0.004040024977540646	2.125121455167867
>5k	4.48891664171183E-4	1.1165697830939254
>10k+	4.48891664171183E-4	1.9265334295944638
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16783	1.9225173831861346	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	9727	1.1142421847257065	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	4994	0.5720700596813179	No Hit
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT	2419	0.27710001489169156	RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	2249	0.257626264361891	RNA PCR Primer, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	1927	0.22074068982897466	RNA PCR Primer, Index 16 (95% over 24bp)
GGGGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	1480	0.16953618108296964	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG	1467	0.16804701192480842	No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1458	0.16701604866146602	RNA PCR Primer, Index 16 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1402	0.16060116613400233	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1117	0.1279539961281602	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAACCCATGTCG	981	0.11237499570431973	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	972	0.11134403244097736	RNA PCR Primer, Index 14 (95% over 21bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	942	0.10790748822983609	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	914	0.10470004696610423	No Hit
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	904	0.10355453222905713	RNA PCR Primer, Index 14 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28500406657731653	0.0
2	0.0	0.0	0.0	0.9591394893295302	0.0
3	0.0	0.0	0.0	1.5282312106945255	0.0
4	0.0	0.0	0.0	2.2281407150302988	0.0
5	0.0	0.0	0.0	4.22477290170338	0.0
6	0.0	0.0	0.0	6.155308888048845	0.0
7	0.0	0.0	0.0	7.7621224097048005	0.0
8	0.0	0.0	0.0	10.639197223272278	0.0
9	0.0	0.0	0.0	12.195378993550753	0.0
10	0.0	0.0	0.0	13.789477301625485	0.0
11	0.0	0.0	0.0	16.096200327617215	0.0
12	1.1455147370470922E-4	0.0	0.0	17.38478985532149	0.0
13	1.1455147370470922E-4	0.0	0.0	17.93601154678855	0.0
14	1.1455147370470922E-4	0.0	0.0	18.274740254533373	0.0
15	1.1455147370470922E-4	0.0	0.0	18.627673345017584	0.0
16	1.1455147370470922E-4	0.0	0.0	19.24544944270708	0.0
17	1.1455147370470922E-4	0.0	0.0	20.021306574109076	0.0
18	1.1455147370470922E-4	0.0	0.0	20.82121951498906	0.0
19	2.2910294740941843E-4	0.0	0.0	21.32398593307903	0.0
20	2.2910294740941843E-4	0.0	0.0	21.88082064675762	0.0
21	2.2910294740941843E-4	0.0	0.0	22.435020676541004	0.0
22	2.2910294740941843E-4	0.0	0.0	23.059440759705375	0.0
23	2.2910294740941843E-4	0.0	0.0	23.635634672440062	0.0
24	2.2910294740941843E-4	0.0	0.0	24.054549411778183	0.0
25	2.2910294740941843E-4	0.0	0.0	24.468194783325888	0.0
26	2.2910294740941843E-4	0.0	0.0	24.84380906560363	0.0
27	2.2910294740941843E-4	0.0	0.0	25.364101859170418	0.0
28	2.2910294740941843E-4	0.0	0.0	25.753691421240134	0.0
29	2.2910294740941843E-4	0.0	0.0	26.2490119935393	0.0
30	2.2910294740941843E-4	0.0	0.0	27.246526226559904	0.0
31	2.2910294740941843E-4	0.0	0.0	27.725809592540408	0.0
32	2.2910294740941843E-4	0.0	0.0	28.08779224944729	0.0
33	2.2910294740941843E-4	0.0	0.0	28.489867922150818	0.0
34	2.2910294740941843E-4	0.0	0.0	28.944637272758513	0.0
35	2.2910294740941843E-4	0.0	0.0	29.375694468309334	0.0
36	2.2910294740941843E-4	0.0	0.0	29.738364434058443	0.0
37	2.2910294740941843E-4	0.0	0.0	30.1483441584476	0.0
38	2.2910294740941843E-4	0.0	0.0	30.64045728948303	0.0
39	2.2910294740941843E-4	0.0	0.0	32.1528803968063	0.0
40	2.2910294740941843E-4	0.0	0.0	32.58668682772604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	70	0.0	46.000004	2
TTCGTCA	35	1.01919795E-7	46.000004	28
GAGCGAC	70	0.0	46.000004	18
TCTCGCG	35	1.01919795E-7	46.000004	32
TCGTCAC	35	1.01919795E-7	46.000004	29
AGCGATA	35	1.01919795E-7	46.000004	34
CGATACG	35	1.01919795E-7	46.000004	36
ACAACGC	30	1.8608862E-6	46.0	18
CTATGCG	30	1.8608862E-6	46.0	1
AACCGAT	20	6.310617E-4	46.0	33
GCGACTA	30	1.8608862E-6	46.0	40
CGTATTG	25	3.416476E-5	46.0	19
CGTATCG	20	6.310617E-4	46.0	25
CAACGTT	40	5.6079443E-9	46.0	14
CGCGGGT	20	6.310617E-4	46.0	4
ACGTTAG	25	3.416476E-5	46.0	1
TAGGTTA	20	6.310617E-4	46.0	39
TTTCGCG	20	6.310617E-4	46.0	1
TTTCGCA	40	5.6079443E-9	46.0	14
TAGCGTA	20	6.310617E-4	46.0	16
>>END_MODULE